Influence of Sulfuric Acid Pretreatment and Inhibitor of Sugarcane Bagasse on the Production of Fermentable Sugar and Ethanol

2021 ◽  
Author(s):  
Elizabeth Jayex Panakkal ◽  
Malinee Sriariyanun ◽  
Jakaphan Ratanapoompinyo ◽  
Patchanee Yasurin ◽  
Kraipat Cheenkachorn ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Acid Concentration ◽  
Sugarcane Bagasse ◽  
Agricultural Waste ◽  
Reducing Sugar ◽  
Alternative Methods ◽  
Acid Pretreatment ◽  
Box Behnken Design ◽  
Sulfuric Acid Pretreatment ◽  
Pretreated Biomass

Improper disposal of agricultural waste after harvesting season has posed serious health and environmental issues. Alternative methods to utilize agricultural waste to produce a value-added product, especially biofuel, have become the focus of research and industrial stakeholders. To make the process feasible, the maximum conversion should be achieved with the optimum operational condition. This research applied Response Surface Methodology (RSM) with the Box-Behnken design (BBD) to optimize sulfuric acid pretreatment of sugarcane bagasse by varying three pretreatment factors namely, acid concentration (0.5–3.5%), temperature (60–140℃), and time (20–100 min). Pretreated biomass was enzymatically hydrolyzed, and the effectiveness of pretreatment was examined according to the reducing sugar concentration. However, inhibitors namely, acetic acid, 5-hydroxymethylfurfural (5-HMF), and furfural were produced during pretreatment, which was analyzed through GC-MS analysis. The Box-Behnken design could optimize and correlate the effect of pretreatment parameters on the hydrolysis of sugarcane bagasse. The optimum pretreatment condition was predicted at an acid concentration of 3.50%, the temperature of 136.08℃, and the time of 75.36 min to obtain the maximum sugar production. Sugarcane bagasse pretreatment at optimum condition could produce a reducing sugar of 180.15 mg/g-sugarcane bagasse, which is 3.06 folds higher than untreated sugarcane bagasse. However, ethanol yield from pretreated biomass was less than unpretreated biomass because of the inhibitor formation. This study provides a new insight into utilizing agricultural waste in a more efficient and eco-friendly manner.

Freeze-Thaw and Sulfuric Acid Pretreatment of Wheat Straw for Fermentable Sugar Release

2013 ◽  
Vol 724-725 ◽  
pp. 257-260 ◽  
Author(s):  
Xin Ming Wang ◽  
Lian Jie Wang ◽  
Meng Yu ◽  
Hui Chen
Keyword(s):  
Sulfuric Acid ◽  
Wheat Straw ◽  
Reducing Sugar ◽  
Acid Pretreatment ◽  
Sugar Release ◽  
Freeze Thaw ◽  
Treatment Conditions ◽  
Sulfuric Acid Pretreatment ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Hydrolysis of cellulose and hemicelluloses of lignocellulosic materials to obtain reducing sugar can be used to produce ethanol by microbial fermentation. Effective pretreatment is necessary for optimal hydrolysis. This study investigated the positive effect of freeze-thaw treatment on low temperature sulfuric acid pretreatment for convert wheat straw to reducing sugar. Freeze-thaw treatment conditions were optimized: at -20°C for 12h, and at room temperature (25°C) for 1h, followed. After twice repeating of freeze-thaw treatment and pretreatment with 2wt% sulfuric acid for 16h at 80°C and enzymatic digestibility with 20U/g of cellulase loading, 67% cellulose and hemicelluloses were converted to glucose and xylose. The yield of furfural was decreased by 65% during sulfuric acid pretreatment. The time of acid hydrolysis was shortened by 20%.

Dilute sulfuric acid pretreatment of cardoon for ethanol production

2008 ◽  
Vol 42 (1) ◽  
pp. 84-91 ◽  
Author(s):  
Ignacio Ballesteros ◽  
Mercedes Ballesteros ◽  
Paloma Manzanares ◽  
M. José Negro ◽  
J. Miguel Oliva ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Ethanol Production ◽  
Acid Pretreatment ◽  
Dilute Sulfuric Acid ◽  
Dilute Sulfuric Acid Pretreatment ◽  
Sulfuric Acid Pretreatment

scholarly journals Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Preliminary hydrolysis and fermentation studies

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
WHINY HARDIYATI ERLIANA ◽  
Tri Widjaja ◽  
ALI ALTWAY ◽  
LILY PUDJIASTUTI
Keyword(s):  
Lactic Acid ◽  
Lignin Content ◽  
Agricultural Waste ◽  
Lactic Acid Production ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Brevis ◽  
Reducing Sugar ◽  
Cellulose Content ◽  
Acid Pretreatment ◽  
Acid Fermentation

Abstract. Erliana WH, Widjaja T, Altway A, Pudjiastuti L. 2020. Synthesis of lactic acid from sugar palm trunk waste (Arenga pinnata): Hydrolysis and fermentation studies. Biodiversitas 21: 2281-2288. The increasing problems of global energy and the environment are the main reasons for developing products with new techniques through green methods. Sugar palm trunk waste (SPTW) has potential as agricultural waste because of its abundant availability, but it is not used optimally. This study was aimed to determine the effect of various microorganisms on increasing lactic acid production by controlling pH and temperature conditions in the fermentation process. SPTW contains 43.88% cellulose, 7.24% hemicellulose, and 33.24% lignin. The lignin content in SPTW can inhibit reducing sugar formation; the pretreatment process should remove this content. In the study, the pretreatment process was conducted using acid-organosolv. In the acid pretreatment, 0.2 M H2SO4 was added at 120oC for 40 minutes; organosolv pretreatment using 30% ethanol (v/v) at 107oC for 33 minutes was able to increase cellulose content by 56.33% and decrease lignin content by 27.09%. The pretreatment was followed by an enzymatic hydrolysis process with a combination of commercial cellulase enzymes from Aspergillus niger (AN) and Trichoderma reesei (TR), with variations of 0:1, 1:0, 1:1, 1:2 and 2:1. The best reducing sugar concentration was obtained with an AN: TR ratio of 1:2 to form reducing sugar from cellulose. Subsequently, lactic acid fermentation was carried out using lactic acid bacteria at 37oC and pH 6 incubated for 48 hours. The highest lactic acid concentration (33.292 g/L) was obtained using a mixed culture of Lactobacillus rhamnosus and Lactobacillus brevis to convert reducing sugar become lactic acid.

scholarly journals Effect of Subsequent Dilute Acid and Enzymatic Hydrolysis on Reducing Sugar Production from Sugarcane Bagasse and Spent Citronella Biomass

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Robinson Timung ◽  
Narendra Naik Deshavath ◽  
Vaibhav V. Goud ◽  
Venkata V. Dasu
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sugarcane Bagasse ◽  
Crystallinity Index ◽  
Sem Analysis ◽  
Reducing Sugar ◽  
Dilute Acid Pretreatment ◽  
Crystalline Cellulose ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Hydrolysis Of

This work was aimed at investigating the effect of process parameters on dilute acid pretreatment and enzymatic hydrolysis of spent citronella biomass (after citronella oil extraction) and sugarcane bagasse on total reducing sugar (TRS) yield. In acid pretreatment, the parameters studied were acid concentration, temperature, and time. At the optimized condition (0.1 M H2SO4, 120°C, and 120 min), maximum TRS obtained was 452.27 mg·g−1and 487.50 mg·g−1for bagasse and citronella, respectively. Enzymatic hydrolysis of the pretreated biomass usingTrichoderma reesei26291 showed maximum TRS yield of 226.99 mg·g−1for citronella and 282.85 mg·g−1for bagasse at 10 FPU, 50°C, and 48 hr. The maximum crystallinity index (CI) of bagasse and citronella after acid pretreatment obtained from X-ray diffraction analysis was 64.41% and 56.18%, respectively. Decreased CI after enzymatic hydrolysis process to 37.28% and 34.16% for bagasse and citronella, respectively, revealed effective conversion of crystalline cellulose to glucose. SEM analysis of the untreated and treated biomass revealed significant hydrolysis of holocellulose and disruption of lignin.

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