scholarly journals Callus Formation on Endosperm from Immature and Mature Fruits of Barringtonia Racemosa

2019 ◽  
Vol 7 (4.14) ◽  
pp. 107
Author(s):  
D S M Soder ◽  
D N A A Khalid ◽  
A Saleh ◽  
F Pardi ◽  
N J Sidik
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Chemical Compounds ◽  
Dichlorophenoxyacetic Acid ◽  
Maturity Stage ◽  
Ms Medium ◽  
Fresh Weight ◽  
Pharmacological Activities ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Barringtonia racemosa is mangroves type of plant which had been extensively utilized in conventional practices for relieving ailments of pain and inflammation. Many studies have been done on ethnobotanical profiles, pharmacological activities and chemical compounds in Barringtonia racemosa. However, there is a limited study on callogenesis of this plant particularly from different maturity stage of fruits. The present study is to identify the callogenesis of Barringtonia racemosa from endosperm explants of immature and mature fruits in MS medium supplemented with different concentrations of hormones 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 0.5, 1.0, 1.5 and 2.0 mg/L) and Kinetin (KIN) (0, 0.5, 1.0, 1.5 and 2.0 mg/L). The optimum hormone combination was found in callus grown on endosperm of immature fruits in MS medium supplemented with 1.5 mg/L 2,4-D and 1.0 mg/L KIN. It was also found that the callus in this treatment grew profusely with highest fresh weight (0.513 ± 0.022 g), 100% callus induction and friable callus texture. The callus fresh weight on endosperm explants was higher in immature fruits compared to mature fruits for all the hormone combinations. Therefore, callogenesis were found more efficient from endosperm explant of immature fruits in Barringtonia racemosa species.   

scholarly journals PERTUMBUHAN KALUS DAUN MELON (Cucumis melo) VARIETAS MAI 119 DENGAN PEMBERIAN BAP (BENZYL AMINO PURINE) DAN 2,4-D (2,4 DICHLOROPHENOXYACETIC ACID)

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nining Intan Toharah ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Growth Regulators ◽  
Callus Induction ◽  
Cucumis Melo ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Benzyl Amino Purine

This study aims to determine the concentration of growth regulators BAP and   2,4-D which have the highest effect in stimulating the formation of callus melon plants (Cucumis melo) Mai 119 variety. Completely randomized design (CRD) was used in this research. Media used on callus induction was MS medium with addition of several concentration of BAP  (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) and 2,4-D (0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L) either alone or in a combination of both. Parameters measured were the time appearing of callus, callus diameter, callus texture, and callus color. Anova followed by Tukey's test was used to the analyse of time appearing of callus. Data of callus diameter was analyzed using Kruskal Wallis test followed by Mann-Whitney test. In the analysis of parameter related to the callus texture and callus color, descriptive test were used. The results showed that there were differences in the effect of growth regulators on the callus formation. The fastest callus induction and the largest diameter of callus were obtained on media with concentration of 2 mg/L BAP and 3 mg/L BAP.Keywords: BAP (benzyl amino purine), 2,4-D (2,4-dichlorophenoxyacetic acid), callus induction, melon (Cucumis melo) varieties Mai 119

scholarly journals High frequency callus induction and proliferation of MD2 pineapple (Ananas comosus)

Food Research ◽  
2020 ◽  
Vol 4 (S5) ◽  
pp. 115-123
Author(s):  
A.N. Salihan ◽  
N.A. Yusuf
Keyword(s):  
Callus Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Ananas Comosus ◽  
Dichlorophenoxyacetic Acid ◽  
Fresh Weight ◽  
Metabolites Production ◽  
2,4 Dichlorophenoxyacetic Acid

Ananas comosus var. MD2 is currently the most preferred pineapple variety in the international market due to its pleasant aroma and high Brix acidity ratio. In vitro approaches such as callus culture is promising in producing disease-free plantlet. However, there are limited studies reported on callus culture of MD2 variety despite the potential of in vitro regeneration through biotechnological advances. The purpose of the study was to determine the effect of plant growth regulators (PGRs) i.e., 2,4- dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP) and Thidiazuron (TDZ) on callus induction from leaf explant of MD2 pineapple. Leaf base explants were cultured on Murashige and Skoog (MS) media supplemented with varying concentration of 2,4-D (0.5 to 6.0 mg/L) alone and in combination with BAP (1.0 to 3.0 mg/L). The frequency of callus induction was seen significantly highest (91.67±8.33%) with maximum callus fresh weight (0.25±0.07 g) at a combination of 2.0 mg/L 2,4-D and 2.0 mg/L BAP. The shortest duration of callus formation was seen on day 12 with the lowest concentration of 2,4-D, 0.5 mg/L. There is a moderate correlation between the earliness of callus formation and the frequency of callus induction (P<0.01). The most favourable media for callus proliferation was 6.0 mg/L 2,4-D and 2.0 mg/L TDZ as the highest fresh weight of 1.52±0.03 g was recorded. Callus culture has the potential to be a source of plant material and secondary metabolites production. In this study, 2,4-D and BAP have successfully induced callus in MD2 pineapple.

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

scholarly journals Callus induction and plant regeneration in the moss Aloina Aloides (Schultz) Kindb. (Pottiaceae, Bryopsida)

2003 ◽  
Vol 55 (3-4) ◽  
pp. 77-80 ◽  
Author(s):  
Aneta Bijelovic ◽  
Marko Sabovljevic
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Basal Medium ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Air Humidity ◽  
Moss Species ◽  
Bud Formation ◽  
Long Period ◽  
2,4 Dichlorophenoxyacetic Acid

Callus induction of moss species Aloina aloides (Schultz) Kindb. was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) or with 1.0 mg/L 2,4-D and 1.0 mg/L kinetin (KIN) or with 0.2 mg/L indole-3-butyric acid (IBA) and 2.0 mg/L 6-benzylaminopurine (BAP) or with 7.5 g/L of sucrose or with 15 g/L of sucrose or hormone - free and sugar free MS basal medium. The callus can be maintained for a long period of time without bud formation subcultured on the above media, at 16 h day/8 h night, 25 ? 2?C, 60-70% air humidity and irradiance of 50 ?mol m-2s-1. To obtain plant regeneration pieces, calli were transferred onto MS media supplemented with different concentrations of auxins and cytokinins (1.0 mg/L 2,4-D and 2 mg/L KIN; 0.2 mg/L IBA and 2 mg/L KIN; or 0.2 mg/L IAA and 2 mg/L BAP). In these media after subculturing, callus enlarges and turns to gametophytes with buds. Except for a smaller size, the plants obtained on the callus did not differ morphoanatomically from the shoots in the nature.

scholarly journals Embryogenic Callus Induction of Aquilaira Malaccensis Lam. and Aquilaria Subintegra Ding Hou

2019 ◽  
Vol 9 (1) ◽  
pp. 5746-5751
Keyword(s):  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Aseptic Culture ◽  
Aquilaria Malaccensis ◽  
The Family ◽  
Commercially Important ◽  
Embryogenic Callus Induction ◽  
2,4 Dichlorophenoxyacetic Acid

Aquilaria malaccensis Lam. and Aquilaria subintegra Ding Hou belong to the family of Thymelaeaceae which is commonly known as gaharu or agarwood. It is a commercially important tree and identified as a potential aromatic plant. The overwhelming responses in the lodging sector reduce gaharu species in the forest. Mass propagation through plant tissue culture technology will substitute this problem. The present study was conducted to investigate the embryogenic callus induction between these two species. The most optimum sterilization method for both species was sodium hypochlorite 5.0% which gave the highest percentage of aseptic culture (95%) with the absence of tissue browning. The leaves of both species were cultured on Murashige and Skoog, (1962) (MS) media supplemented with combination of various concentrations of 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 2.5 mg/L) and 2,4-dichlorophenoxyacetic acid (2, 4-D) (0.5, 1.0, 1.5 and 2.0 mg/L) and kept under dark condition. The explants produced embryogenic, white and compact callus at the end cut of the explants after two weeks of culture in all treatments. The highest frequency of embryogenic callus formation was observed in explants cultured on 2.0 mg/L BAP and 0.5 mg/L 2,4-D for both species. From the present study, the optimum sterilization technique and embryogenic callus induction for A. malaccensis Lam. and A. subintegra were established.

scholarly journals In Vitro Regeneration Of Brinjal (Solanum Melongena L.)

1970 ◽  
Vol 36 (3) ◽  
pp. 397-406 ◽  
Author(s):  
BP Ray ◽  
L Hassan ◽  
KM Nasiruddin
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Solanum Melongena ◽  
Ms Medium ◽  
Fresh Weight ◽  
Normal Environment ◽  
Regenerated Plantlets

The effect of different explants and concentrations of BAP and NAA on induction of callus and plant regeneration of brinjal cv. Jhumki were investigated. The treatment combinations were BAP (0. 2.0. 3.0, and 4.0 mg/l) and NAA (0. 0.1, 0.5, and 1.0 mg/l). The rate of callus formation varied in different treatments. The highest amount of callus (48.66%) was produced on MS medium containing 2.0 mg/l BAP and 0.5 mg/l NAA from stem, and 8.2 days required for callus induction. The highest fresh weight of callus was 1.12g from stem and 0.48g from root. The number of shoot regenerated through callus from stem containing 2.0 mg/l BAP and 0.5 mg/l NAA was 3.4 (23.287%) and days required for 38.8 days. All regenerated plantlets survived in normal environment. Keywords: NAA; BAP; regeneration; brinjal. DOI: http://dx.doi.org/10.3329/bjar.v36i3.9268 BJAR 2011; 36(3): 397-406

scholarly journals Improvement of friable callus production of Boerhaavia paniculata Rich and the investigation of its lipid profile by GC/MS

2014 ◽  
Vol 86 (3) ◽  
pp. 1015-1027 ◽  
Author(s):  
JOANNE M.M. SOUZA ◽  
STRAHILL BERKOV ◽  
ALBERDAN S. SANTOS
Keyword(s):  
Lipid Profile ◽  
Callus Formation ◽  
Wild Plant ◽  
Dichlorophenoxyacetic Acid ◽  
Tetradecanoic Acid ◽  
Friable Callus ◽  
Murashige And Skoog Medium ◽  
Callus Production ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Tetracosanoic Acid

In this study, a protocol to induce high amount of friable callus of Boerhaavia paniculata RICH and a lipidomics technique were applied to investigate the profile of lipids to relate to those present in the roots of this plant that presented anti-inflammatory activity in the crude hexane extract. The callus culture was induced from seeds in solidified Murashige and Skoog medium containing different amounts of glucose and different concentrations of 2,4-Dichlorophenoxyacetic acid. The explants were kept in a germination chamber at 30±2°C with a photoperiod of 16 h under light intensity of 27 µmol m–2 s–1 for 4 weeks. The best results for friable callus formation and development of the biomass were obtained in the treatment containing 2.26 µM 2.4-D and glucose (1.5 %; w/v). Lipidomics techniques were applied in hexane fraction showing higher concentrations of the steroids β-sitosterol (3.53 mg/100 g dc–dry cells), and fatty acids, especially 2-hydroxy-tetracosanoic acid (0.34 mg/100 g dc), eicosanoic acid (86.25 mg/100 g dc), stearic acid (420.83 mg/100 g dc), tetradecanoic acid (10.74 mg/100 g dc) and linoleic acid (100.61 mg/100 g dc). The lipid profile of callus versus that found in the roots of wild plant is described in this work.

scholarly journals Comparing the effects of different exogenous hormone combinations on seed-derived callus induction in Nicotiana tabacum

2020 ◽  
Author(s):  
Jakub Lang ◽  
Jędrzej Pankowski ◽  
Piotr Grabarz ◽  
Bartosz Pluciński ◽  
Paweł Jedynak
Keyword(s):  
Nicotiana Tabacum ◽  
Photosystem Ii ◽  
Callus Induction ◽  
Indoleacetic Acid ◽  
Callus Formation ◽  
Dichlorophenoxyacetic Acid ◽  
Exogenous Hormone ◽  
Stress Symptoms ◽  
Fluorescence Of Chlorophyll A ◽  
2,4 Dichlorophenoxyacetic Acid

Callus from Nicotiana tabacum is used as a model in plant developmental research. We tested several phytohormone (Indoleacetic acid – IAA; 2,4-Dichlorophenoxyacetic acid – 2,4-D; kinetin – KIN; 6-Benzylaminopurine – BAP) combinations to compare different approaches to callus induction directly from the seeds of Nicotiana tabacum. Callus formation was observed up to 4 weeks after sowing and the most effective were 0.5 mg/L of 2,4-D with 0.25 mg/L of BAP and 2 mg/L 2,4-D with 1 mg/L of BAP. The calli were green, photosynthetically active and after 6 weeks of growth, no stress symptoms (estimated on the basis of fluorescence of chlorophyll a in photosystem II) were noticed.

scholarly journals The Effects of 2,4-Dichlorophenoxyacetic Acid and α-Naphthaleneacetic Acid on Biomass Increment, Rhizogenesis and Somatic Embryogenesis of Suspension-cultured Dinh Lang Cells [Polyscias fruticosa (L.) Harms]

2021 ◽  
Author(s):  
T.T.B. Phuong ◽  
V.P. Trung ◽  
N.H. An ◽  
N.D. Tuan ◽  
P.T.T. Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Bioactive Compound ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Fresh Weight ◽  
Triterpenoid Saponins ◽  
Cell Biomass ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Dinh Lang [Polyscias fruticosa (L.) Harms] is a medicinal plant widely grown in Vietnam, with proven note-worthy health benefits. However, Dinh Lang’s amounts of triterpenoid saponins could not meet the need of the pharmaceutical industry. Thus, this study’s purpose is to figure out the optimal condition for raising Dinh Lang’s cell biomass, rhizogenesis and somatic embryogenesis to provide materials for bioactive compound productions. Methods: Different 2,4-dichlorophenoxyacetic acid and α-naphthaleneacetic acid concentrations (0.5, 1.0, 1.5 and 2.0 mg/L) were examined to determine the best amount of each plant growth regulator for raising cells’ biomass, rhizogenesis and somatic embryogenesis. In each treatment, two grams of eight-week-old calli were cultured in 50 mL of liquid MS medium. Result: It is demonstrated by the results that liquid MS medium containing 1.5 mg/L α-naphthaleneacetic acid has the capacity of producing the highest numbers of somatic embryos (489 embryos per flask) and rooted cells (259.5 cells per flask), while the fresh weight of cells cultured in the medium given 1.5 mg/L 2,4-dichlorophenoxyacetic acid reached its peak of 5.7 g.

scholarly journals Callus induction of mountain papaya endosperm (Vasconcellea pubescens A. DC) with different combination of 2,4-Dichlorophenoxyacetic acid and Kinetin

2022 ◽  
Vol 951 (1) ◽  
pp. 012072
Author(s):  
R K Zuhro ◽  
H A Dewanto ◽  
A Suyadi ◽  
T Pribadi ◽  
O D Hadjoeningtijas ◽  
...  
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Block Design ◽  
Dichlorophenoxyacetic Acid ◽  
Double Fertilization ◽  
Randomized Block Design ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
High Level ◽  
Experimental Group ◽  
Dead Tissue

Abstract Endosperm as a result of double fertilization in Angiospermae shows high level chromosomes and polyploidy. It is also considered as dead tissue that unable to be generated to form plantlet. The aim of this research is to determine the effect of kinetin and 2,4-Dichlorophenoxyacetic acid (2,4-D) in induction of callus formation of mountain papaya. This research used A factorial randomized block design with 18 groups, 1 fruit was used for 1 experimental group. Culture using Murashige and Skog (MS) media with combination of three level of kinetin (0, 2, 4 mgL-1) and six level of 2,4-D (0, 1, 2, 3, 4, 5 mgL-1). Maximum endosperm callus induction (0.88%) was achieved from endosperm explant cultured on MS medium fortified with 2.0 mgL-1 Kinetin and 4.0 mgL-1 2,4-D. The fastest day induction (24,66 day) was observed with 5.0 mg L-1 2,4-D. The maximum number of browning (0,10) was induced by 2.0 mgL-1 Kinetin and 5.0 mgL-1 2,4-D. Combination Kinetin and 2,4-D proved could induces callus formation from mountain papaya endosperm.

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