The Abnormal Metabolites in Tongue Coating of Chronic Gastritis Patients Reflect the Changes in Indexes of Gastroscopic Observation and Gastric Mucosal Pathology

ObjectiveIn this study, we analyzed the correlation between different metabolites in the tongue coating of patients with chronic gastritis, gastroscopy and pathological indexes, and discussed the metabolic mechanism at different pathological stages in the development of chronic gastritis.MethodsWe used GC-TOF-MS and UHPLC-QE-MS metabonomics to detect the distribution of metabolites in the tongue coating of patients with chronic gastritis, and analyzed the correlation between different metabolites in the tongue coating of patients with chronic gastritis and gastroscopy and pathological indexes.ResultsCompared with 50 healthy people, 54 metabolites were upregulated and 47 metabolites were downregulated in 350 patients with chronic gastritis. The main differential metabolites were Lipids and lipid-like molecules, which contain 47 metabolites. The best diagnostic model was composed of 5 metabolites, with an accuracy of 95.4%, a specificity of 87.4% and a sensitivity of 88.0%. These 5 metabolites were 1-methyladenosine, Sphinganine 1-phosphate, 3-Hydroxycapric acid, 4-Ipomeanol, and Nervonic acid. Compared with healthy people, Sphinganine 1-phosphate, 4-Ipomeanol, and Nervonic acid were significantly upregulated in chronic gastritis patients, and 1-methyladenosine and 3-Hydroxycapric acid were significantly downregulated in chronic gastritis patients. After correlation analysis between differential metabolites in tongue coatings and gastroscopic indexes, we found that Trimethylaminoacetone, Sphinganine1-phosphate, alpha-Carboxy-delta-decalactone, and 5,6-Dihydroxyindole were positively correlated with intestinal metaplasia. Conduritol-beta-expoxide, Tetracosanoic acid, Lactosylceramide(d18:1/26:0), Chondrillasterol 3-[glucosyl-(1->4)-glucoside], Azelaic acid, and 1-Methyladenosine were negatively correlated with intestinal metaplasia. Sphinganine1-phosphate, alpha-Carboxy-delta-decalactone, and 5,6-Dihydroxyindole were positively correlated with atrophic. Octadecanol, conduritol-beta-expoxide, Tetracosanoic acid, Smilanippin A, Lactosylceramide(d18:1/26:0), Chondrillasterol 3-[glucosyl-(1->4)-glucoside], and Azelaic acid were negatively correlated with atrophic. 6-deoxyglucitol was negatively correlated with bile reflux, methylmaleic acid, 4-methylcatechol, and 2,4-dichloro-1-(2-chloroethenyl)-benzene were negatively correlated with Hp, 3-benzoyloxy-11-oxo-12-ursen-28-oic acid was negatively correlated with gastric mucosal erosion. From the change trend of different metabolites in different pathological stages, we found that the content of conduritol-beta-expoxide decreased significantly in mild atrophic compared with moderate atrophic and the content of conduritol-beta-expoxide decreased significantly in mild intestinal metaplasia compared with moderate intestinal metaplasia.ConclusionsWe found that Lipids and lipid-like molecules were the main abnormal metabolites in patients with chronic gastritis. Among them, Sphinganine 1-phosphate, which was significantly positively correlated with the aggravation of atrophic and intestinal metaplasia, could be used as one of the diagnostic model markers for chronic gastritis. Additionally, the amount of conduritol-beta-expoxide significantly decreased with the aggravation of atrophic and intestinal metaplasia. We believe that these differential markers in tongue coating may help us to establish a noninvasive and convenient auxiliary detection method for gastritis and gastric precancerous lesion in the future.

Isolasi, karakterisasi, identifikasi, dan uji aktivitas antibakteri trigliserida biji mangga golek (Mangifera indica Linn)

There has been research on the isolation, characterization, and identification of triglyceride seeds of mango golek (Mangiifera indica Linn) and its activity as antibacterial. Isolation process by maceration and soxhlet produces smooth component as triglycerides and was creamy white in colour. These golek mango seed kernel triglicerides has melting point 34-36 degree celcius, soluble in hexane, chloroform, acetone, and ethyl acetate, slightly soluble in methanol and insoluble in water, has a carbon-carbon double bond, acid number of 2.8, saponification number of 336.6, and iodine number of 25.4. The GC-MS anlysis of methyl ester shown fatty acids contain in golek mango seed kernel triglicerides. The fatty acids are hexadecanoic (26.31 percent), heptadecanoic (0.6 percent), 9-octadecenoic (28.70 percent), octadecanoic (25.86 percent), 11-eicosenoic (1.74 percent), eicosenoic (11.20 percent), docosanoic (2.47 percent), and tetracosanoic acid (2.39 percent). Triglycerides of golek mango seed kernel has no potential as an antibacterial against Escherichia coli and Staphylococcus aureus. Telah dilakukan penelitian mengenai isolasi, karakterisasi, dan identifikasi trigliserida biji mangga golek (Mangiifera indica Linn) serta aktivitasnya sebagai antibakteri. Isolasi dilakukan dengan maserasi dan soxhletasi menggunakan aseton diperoleh komponen padatan lunak berwarna putih kekuningan. Trigliserida biji mangga golek ini mempunyai titik lebur 34-36 derajat celcius, larut dalam heksana, kloroform, aseton, dan etil asetat, sedikit larut dalam metanol, tidak larut dalam air, memiliki ikatan rangkap C=C, mempunyai bilangan asam 2,8, bilangan penyabunan 336,6, dan bilangan iod 25,4. Analisis secara GC-MS terhadap metil ester hasil trans-esterifikasi trigliserida biji mangga golek diperoleh informasi asam lemak penyusunnya. Asam-asam lemak tersebut adalah asam heksadekanoat (26,31 persen), heptadekanoat (0,60 persen), 9-oktadekenoat (28,70 persen), oktadekanoat (25,86 persen), 11-eikosenoat (1,74 persen), eikosenoat (11,20 persen), dokosanoat (2,47 persen), dan tetrakosanoat (2,39 persen). Trigliserida biji mangga golek tidak memiliki aktivitas antibakteri terhadap Escherichia coli dan Staphylococcus aureus.

ANTIMICROBIAL AND OTHER BIOACTIVE CONSTITUENTS OF Cannabis sativus ROOTS FROM PAKISTAN

Hemp (Cannabis sativa L.), a medicinal plant of family Cannabaceae, growing wildly in most of the rainfed areas of Punjab, Pakistan. In this study, phytochemical profile of methanolic extract of root was assessed through GC-MS analysis and various biologically active compounds were identified through literature survey. There were 14 compounds in the root extract. The most abundant compounds were γ-sitosterol (27.08%) and 9,12-octadecadienoic acid (Z,Z)-, methyl ester (24.09%) and hexadecanoic acid, methyl ester (21.81%). Other identified compounds were methyl stearate (5.51%), stigmasterol (5.12%), campesterol (4.19%), phenol, 2,2'-methylenebis[6-(1,1-dimethylethyl)-4-methyl-(2.52%), 11-octadecenoic acid, methyl ester (2.10%), squalene (1.89%), β-amyrin (1.88%), eicosanoic acid, methyl ester (0.85%), tetracosanoic acid, methyl ester (0.88%), dronabinol (1.02%) and 2-methoxy-4-vinylphenol (0.96%). Most of the identified compounds possess one or more biological activities viz. antitumor, antifungal, antibacterial, antioxidant, anticancer, anti-inflammatory, antidiabetic, and/or analgesic.

Production of Renewable Lipids by the Diatom Amphora copulata

The asymmetric biraphid pennate diatom Amphora copulata, isolated from tropical coastal waters (South China Sea, Malaysia), was cultured for renewable production of lipids (oils) in a medium comprised of inorganic nutrients dissolved in dilute palm oil mill effluent (POME). Optimal levels of nitrate, phosphate, and silicate were identified for maximizing the biomass concentration in batch cultures conducted at 25 ± 2 °C under an irradiance of 130 µmol m−2 s−1 with a 16 h/8 h light-dark cycle. The maximum lipid content in the biomass harvested after 15-days was 39.5 ± 4.5% by dry weight in a POME-based medium with optimal levels of nitrate, phosphate, and silicate. Under the optimized conditions the maximum dry mass concentration of the diatom was 660 mg L−1 on day 12, declining to ~650 mg L−1 on day 15. For the 15-day batch operation, the final average productivities of the biomass and the lipids were 43.3 ± 4.5 mg L−1 d−1 and 17.1 ± 0.3 mg L−1 d−1, respectively. The fatty acids in the diatom lipids were found to be (%, w/w of total lipids): palmitoleic acid (39.8%), palmitic acid (31.9%), myristic acid (6.8%), oleic acid (4.7%), stearic acid (4.5%), arachidonic acid (3.9%), eicosapentaenoic acid (3.6%), linoleic acid (2.5%), tetracosanoic acid (1.7%), and linolenic acid (0.6%).

Novel hydrocarbon-utilizing soil mycobacteria synthesize unique mycocerosic acids at a Sicilian everlasting fire

Soil bacteria rank among the most diverse groups of organisms on Earth and actively impact global processes of carbon cycling, especially in the emission of greenhouse gases like methane, CO2 and higher gaseous hydrocarbons. An abundant group of soil bacteria are the mycobacteria, which colonize various terrestrial, marine and anthropogenic environments due to their impermeable cell envelope that contains remarkable lipids. These bacteria have been found to be highly abundant at petroleum and gas seep areas, where they might utilize the released hydrocarbons. However, the function and the lipid biomarker inventory of these soil mycobacteria are poorly studied. Here, soils from the Fuoco di Censo seep, an everlasting fire (gas seep) in Sicily, Italy, were investigated for the presence of mycobacteria via 16S rRNA gene sequencing and fatty acid profiling. The soils contained high relative abundances (up to 34 % of reads assigned) of mycobacteria, phylogenetically close to the Mycobacterium simiae complex and more distant from the well-studied M. tuberculosis and hydrocarbon-utilizing M. paraffinicum. The soils showed decreasing abundances of mycocerosic acids (MAs), fatty acids unique for mycobacteria, with increasing distance from the seep. The major MAs at this seep were tentatively identified as 2,4,6,8-tetramethyl tetracosanoic acid and 2,4,6,8,10-pentamethyl hexacosanoic acid. Unusual MAs with mid-chain methyl branches at positions C-12 and C-16 (i.e., 2,12-dimethyl eicosanoic acid and 2,4,6,8,16-pentamethyl tetracosanoic acid) were also present. The molecular structures of the Fuoco di Censo MAs are different from those of the well-studied mycobacteria like M. tuberculosis or M. bovis and have relatively δ13C-depleted values (−38 ‰ to −48 ‰), suggesting a direct or indirect utilization of the released seep gases like methane or ethane. The structurally unique MAs in combination with their depleted δ13C values identified at the Fuoco di Censo seep offer a new tool to study the role of soil mycobacteria as hydrocarbon gas consumers in the carbon cycle.

HEXANE SOLUBLE BIOACTIVE COMPONENTS OF Chenopodium murale STEM

Chenopodium murale L. is a common winter weed mostly growing along the road-sides. In the present study, n-hexane soluble fraction of methanolic stem extract of this weed was subjected to GC-MS analysis that showed the presence of 28 compounds in this fraction. Major compounds included oleic acid (16.55%), palmitic acid (11.22%), β-sitosterol (9.63%), hexadecanoic acid (7.71%) and methyl oleate (5.90%). Other prominent compounds were piperine (4.75%), nonacosane (4.69%), monoplalmitin (4.21%), γ-sitosterol (3.91%), methyl linoleate (3.88%), neocurdione (3.86%) and ethanonone (3.25%). The compounds such as stigmasterol (2.92%), 1,4-benzenedicarboxylic acid (2.78%), tetracosanoic acid (1.19%), stearic acid (1.36%), tridecanoic acid (1.35%), tridecanal (1.30%), phytol (1.29%), docosanoic acid, methyl ester (1.23%), octadecanoic acid (1.20%), 4-pyrimidinecarboxylic acid (1.05%), 2-hydroxy-2-phenylbutanamide (0.91%), hexacosanoic acid (0.76%), cholestrol (0.61%), methyl octacosanoate (0.55%) and tetracosanoic acid (0.30%) were present in low concentrations. A thorough literature survey showed that most of the identified compounds possessed antifungal and/or antibacterial properties while very few of them also possessed antioxidant potential. This study concludes that n-hexane soluble fraction of methanolic stem extract of C. murale is a big storehouse of antimicrobial compounds.

Self-Control Study on the Impact of Buzhong Yiqi Prescription on the Gut Microbiota of Obese Patients with PCOS and Phlegm-Dampness Syndrome Caused by Spleen Deficiency

Background: Gut microbiota disorders have been closely related to polycystic ovarian syndrome (PCOS). Buzhong Yiqi prescription (BZYQ) has a great clinical effect on the treatment of obese patients with PCOS and phlegm-dampness syndrome caused by spleen deficiency (SPSD). This study was performed to explore the alterations in the gut microbiota and fecal metabolites in obese patients with PCOS and SPSD who received BZYQ treatment. Methods: A total of 50 obese patients with PCOS and SPSD were recruited from the Changhai Hospital in Shanghai and accepted three months of BZYQ treatment. Sex hormone were detected and oral glucose tolerance was tested in the outpatient laboratory before and after the BZYQ treatment. Fecal samples were detected by 16S rRNA high-throughput sequencing and nontargeted metabolomic methods to determine the structure of the gut microbiota and metabolites, respectively.Results: BZYQ could significantly alleviate the serum DHEAS (p<0.001) and T level (p<0.001) in obese patients with PCOS and SPSD. The structure of the gut microbiota changed significantly after the BZYQ treatment. In particular, at the phylum level, the abundance of Spirochaetae was significantly higher after treatment than that before treatment. At the genus level, the abundances of [Eubacterium]_rectale_group, Escherichia-Shigella, and Fusicatenibacter were significantly higher after treatment than those before treatment, but the abundance of Megamonas was significantly lower. A total of 106 differential metabolites and 14 KEGG enrichment pathways were quantified. The disorder in the gut microbiota and fecal metabolites of obese patients with PCOS and SPSD were closely related to hyperandrogenemia and insulin resistance. The level of tetracosanoic acid was negatively correlated with serum DHEA level (p<0.05), while the palmitoleic acid level was negatively correlated with serum T level (p<0.05). Conclusions: BZYQ could ameliorate the serum androgen level and had an impact on the gut microbiota and metabolites in obese patients with PCOS and SPSD.Trial registration: Chinese Clinical Trial Registry, ChiCTR-IPR-16009166. Registered 26 September 2016, http://www.chictr.org.cn/showproj.aspx?proj=14956

Novel hydrocarbon-utilizing soil mycobacteria synthesize unique mycocerosic acids at a Sicilian everlasting fire

Soil bacteria rank among the most diverse groups of organisms on Earth and actively impact global processes of carbon cycling, especially in the emission of greenhouse gases like methane, CO2 and higher gaseous hydrocarbons. An abundant group of soil bacteria are the mycobacteria, which colonize various habitats due to their impermeable cell envelope that contains remarkable lipids. These bacteria have been found to be highly abundant at petroleum and gas seep areas, where they might utilize the released hydrocarbons. However, the function and the lipid biomarker inventory of these soil mycobacteria are poorly studied. Here, soils from the Fuoco di Censo seep, an everlasting fire (gas seep) in Sicily, Italy, were investigated for the presence of mycobacteria via 16S rRNA gene sequencing and fatty acid profiling. The soils contained high relative abundances (up to 34 % of reads assigned) of mycobacteria, phylogenetically close to the Mycobacterium simiae complex and more distant to the well-studied M. tuberculosis and hydrocarbon-utilizing M. paraffinicum. The soils showed decreasing abundances of mycocerosic acids (MAs), fatty acids unique for mycobacteria, with increasing distance from the seep. The major MAs at this seep were tentatively identified as 2,4,6,8-tetramethyl tetracosanoic acid and 2,4,6,8,10-pentamethyl hexacosanoic acid. Unusual MAs with mid-chain methyl branches at positions C-12 and C-16 (i.e. 2,12-dimethyl eicosanoic acid and 2,4,6,8,16-pentamethyl tetracosanoic acid) were also present. The molecular structures of the Censo MAs are different from those of the well-studied mycobacteria like M. tuberculosis or M. bovis and have relatively 13C-depleted values (−38 to −48 ‰), suggesting a direct or indirect utilization of the released seep gases like methane or ethane. The structurally unique MAs in combination with their depleted δ13C values identified at the Censo seep offer a new tool to study the role of soil mycobacteria as hydrocarbon gas consumers in the carbon cycle.