scholarly journals Simulation of Surface Plasmon Resonance Biosensor Based on Nanoparticle Coreshell in 633 nm

2017 ◽  
Vol 1 ◽  
pp. 81-83
Author(s):  
Widayanti Widayanti ◽  
Abraha K ◽  
Utomo A.B
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Effective Medium Theory ◽  
Dna Detection ◽  
Incident Light ◽  
Active Material ◽  
Attenuated Total Reflection ◽  
Spr Biosensor

In this research, we developed the modeling and simulated the biosensor based on Surface Plasmon Resonance (SPR) phenomenon which detected the analyte i.e DNA. This SPR biosensor consists four layers where one of thus layers is the composite material. This nanocomposite spherical nanoparticle consisting of a spherical Fe3O4@Au core covered by Au shell, were applied as active material for DNA detection in 633 nm. Here, we present the simulation of detection amplification technique through Attenuated Total Reflection (ATR) spectrum. Here, SPR system using the Kretschmann configuration, whereas the dielectric function determination of composite coreshell nanoparticle using Effective Medium Theory approximation. Finally, the reflectivity calculation was investigated by varied the size of the core and the shell of the coreshell. The refractive index of the prism is 1,723; the refractive index of 40 nm Ag thin film is 0.13455+3,98651i and the refractive index of the composites were variated dependent the size of nanoparticle coreshell. Our results show that by varying the radius of core and shell thickness, the peak of the reflectivity (ATR spectrum) was shifted to the different angle of incident light. From its result, we can conclude that the addition of coreshell in SPR biosensor leads to enhancement the biosensor sensitivity in DNA detection.

scholarly journals Surface Plasmon Resonance (SPR) Computational Study of Hemoglobin (Hb) in Human Blood Detection

2018 ◽  
Author(s):  
Wida Yanti ◽  
Kamsul Abraha ◽  
Agung Bambang
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Effective Medium Theory ◽  
Computational Study ◽  
Active Material ◽  
Attenuated Total Reflection ◽  
Core Shell ◽  
The Core

A theoretical analysis of haemoglobin (Hb) concentration detection is presented in this work with the objective of achieving more sensitive detection and monitoring low concentrations. Surface-enhanced SPR spectroscopy on silver nanoparticles was employed for recording Hb concentrations less than 10 g/L. In this paper, Fe3O4@Au core-shell, nanocomposite spherical nanoparticle consisting of a spherical Fe3O4 core covered by Au shell, was used as an active material for biomolecules detection in the Surface Plasmon Resonance (SPR)-based biosensor in the wavelength 632.8 nm. We present the simulation of detection amplification technique through Attenuated Total Reflection (ATR) spectrum in the Kretschmann configuration. The system consists of a four-layer material i.e., prism/Ag/Fe3O4@Au+Hb/air. Dielectric function determination of the core-shell nanoparticle (Fe3O4@Au) and the composite (Fe3O4@Au+Hb) was done by applying the Effective Medium Theory approximation and the calculation of the reflectivity is carried out by varying the size of core-shell (r0). In this simulation, the refractive index of the BK7 prism is 1.51; the refractive index of Ag thin film is 0.13455 + 3.98651i with the thickness of 40 nm, and the refractive index of the composite is varied depending on the size of nanoparticle core-shell. Our results show that by varying the radius of the core and the shell thickness, the dip of the reflectivity (ATR) spectrum is shifted to the larger angle of incident light and the addition of core-shell in the conventional SPR-based biosensor leads to enhancement of the SPR biosensor sensitivity, for the core-shell radius 10 nm, the sensitivity increased by 1.35% for F = 0.1, and by 4.89% for F = 0.8 compared to the sensitivity of the conventional SPR-based biosensor without core-shell addition.

scholarly journals Surface Plasmon Resonance (SPR) Computational Study of Hemoglobin (Hb) in Human Blood Detection

2018 ◽  
Author(s):  
Wida Yanti ◽  
Kamsul Abraha ◽  
Agung Bambang S.U.
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Effective Medium Theory ◽  
Computational Study ◽  
Active Material ◽  
Attenuated Total Reflection ◽  
Core Shell ◽  
The Core

A theoretical analysis of haemoglobin (Hb) concentration detection is presented in this work with the objective of achieving more sensitive detection and monitoring low concentrations. Surface-enhanced SPR spectroscopy on silver nanoparticles was employed for recording Hb concentrations less than 10 g/L. In this paper, Fe3O4@Au core-shell, nanocomposite spherical nanoparticle consisting of a spherical Fe3O4 core covered by Au shell, was used as an active material for biomolecules detection in the Surface Plasmon Resonance (SPR)-based biosensor in the wavelength 632.8 nm. We present the simulation of detection amplification technique through Attenuated Total Reflection (ATR) spectrum in the Kretschmann configuration. The system consists of a four-layer material i.e prism/Ag/Fe3O4@Au+Hb/air. Dielectric function determination of the core-shell nanoparticle (Fe3O4@Au) and the composite (Fe3O4@Au+Hb) was done by applying the Effective Medium Theory approximation and the calculation of the reflectivity is carried out by varying the size of core-shell (r0). In this simulation, the refractive index of the BK7 prism is 1.51; the refractive index of Ag thin film is 0.13455+3.98651i with the thickness of 40 nm, and the refractive index of the composite is varied depending on the size of nanoparticle core-shell. Our results show that by varying the radius of the core and the shell thickness, the dip of the reflectivity (ATR) spectrum is shifted to the larger angle of incident light and the addition of core-shell in the conventional SPR-based biosensor leads to enhancement of the SPR biosensor sensitivity, for the core-shell radius 10 nm, the sensitivity increased by 1.35% for F = 0.1, and by 4.89% for F =0.8 compared to the sensitivity of the conventional SPR-based biosensor without core-shell addition.

scholarly journals Graphene-MoS2-Au-TiO2-SiO2 Hybrid SPR Biosensor: A New Window for Formalin Detection

2019 ◽  
Vol 8 (2) ◽  
pp. 51-58 ◽  
Author(s):  
Md. Biplob Hossain ◽  
M. M. Rahman Khan ◽  
Md. Sadiqur Rahman ◽  
S. S. Bin Badrudduza ◽  
M. M. Sabiha ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Hybrid Structure ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
The Other ◽  
Maximum Sensitivity ◽  
Spr Biosensor

In this article, numerically a surface plasmon resonance (SPR) biosensor is developed based on Graphene-M­­­­OS2-Au-TiO2-SiO2 hybrid structure for formalin detection. This developed sensor sensed the presence of formalin by applying attenuated total reflection (ATR). In ATR method, we developed and observed two characteristics curve, one is “SPR angle versus minimum reflectance (Rmin)” and another is “SPR frequency (SPRF) versus maximum transmittance (Tmax). In the proposed sensor, Chitosan is used as probe legend to perform specific reaction with the formalin (40% formaldehyde) as target legend. Here, graphene and MoS2 both are used as biomolecular acknowledgment element (BAE). And TiO2 as well as SiO2 bilayers are used to improve sensor sensitivity and Gold (Au) is to sharp SPR curve. In numerical results, the variation of SPRF and SPR angle for inappropriate sensing of formalin is quiet insignificant which confirms the absence of formalin. On the other hand, these variations for appropriate sensing is considerably significant that confirms the presence of formalin. At the end of this article, a study of variation of sensitivity of the proposed biosensor in corresponding to the increment of refractive index with a refractive index step 0.01 RIU is measured. In inclusion of TiO2-SiO2 bilayers with Graphene-M­­­­OS2, maximum sensitivity of 85.375% more is numerically reported.

scholarly journals Surface Plasmon Resonance Sensor Based on Polymer Liquid-Core Fiber for Refractive Index Detection

Photonics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 123
Author(s):  
Xuqing Shui ◽  
Qiongchan Gu ◽  
Xiaoxiao Jiang ◽  
Guangyuan Si
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Metal Layer ◽  
Incident Light ◽  
Liquid Core ◽  
Core Structure ◽  
Polymer Optical Fiber ◽  
Resonance Sensor

In this work, a surface plasmon resonance (SPR) sensor based on a novel liquid-core polymer optical fiber (POF) is proposed and numerically analyzed for refractive index (RI) detection. The polytetrafluoroethylene (PTFE) fiber is selected as the platform for SPR sensing. We combine the PTFE-based POF with the liquid-core structure by introducing a hole filled with analyte into the fiber center. The hole also acts as the fiber core to guide the incident light. This design helps to realize the detection of solutions with low RI values (around 1.33), while keeping the distinguished sensing characteristics of the liquid-core structure. Two side air holes are introduced into the cladding and a thin silver film protected by a titanium dioxide layer is plated on the wall of one air hole, which helps to control the mode coupling. In order to optimize the design of this sensor, the impacts of parameters such as metal layer thicknesses and the central hole radius are investigated using the full-vector finite element method (FEM). After optimization, our design shows a wavelength interrogation sensitivity reaching up to 16,750 nm/RIU and an average full-width at half-maximum (FWHM) of 42.86 nm in the RI range of 1.325–1.35.

Performance of Newly-Fabricated Modular Surface Plasmon Resonance (mSPR) Sensor

2016 ◽  
Vol 857 ◽  
pp. 459-464
Author(s):  
Wan Mohd Azwady Wan Ahamad ◽  
Dzaraini Kamarun ◽  
Mohd Kamil Abd Rahman
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Light Source ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Single Channel ◽  
Resonance Wavelength ◽  
Modular Surface ◽  
Angle Variation ◽  
Spr Biosensor

Performance of modular surface plasmon resonance (mSPR) sensor based on refractive index is discussed in this paper. The sensor was built in house using a polychromatic light source, polarizer to produce a transverse magnetic wave, high refractive index waveguide, gold-coated disk, single channel cell and spectrometer for data analysis. A knob for adjusting the angle of the incident provides a means for ease of angle variation which simplifies the design of the instrument for portability purposes. In conventional SPR, the light source need to be delocalized for search of the resonance angle, making the instrument bulky in size and had to be laboratory-based. The efficiency of the newly designed SPR biosensor was tested using a various percentages of ethanol in deionized water. Observations on the shifts of the resonance wavelength with ethanol strength revealed that the SPR biosensor has a sensitivity of 64 nm/RIU and a resolution of ~102 RIU.

scholarly journals Application of Long-Range Surface Plasmon Resonance for ABO Blood Typing

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Wanida Tangkawsakul ◽  
Toemsak Srikhirin ◽  
Kazunari Shinbo ◽  
Keizo Kato ◽  
Futao Kaneko ◽  
...  
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Blood Group ◽  
Long Range ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Refractive Index ◽  
Self Assembled Monolayer ◽  
Blood Typing ◽  
Spr Biosensor

In this study, we demonstrate a long-range surface plasmon resonance (LR-SPR) biosensor for the detection of whole cell by captured antigens A and B on the surface of red blood cells (RBCs) as a model. The LR-SPR sensor chip consists of high-refractive index glass, a Cytop film layer, and a thin gold (Au) film, which makes the evanescent field intensity and the penetration depth longer than conventional SPR. Therefore, the LR-SPR biosensor has improved capability for detecting large analytes, such as RBCs. The antibodies specific to blood group A and group B (Anti-A and Anti-B) are covalently immobilized on a grafting self-assembled monolayer (SAM)/Au surface on the biosensor. For blood typing, RBC samples can be detected by the LR-SPR biosensor through a change in the refractive index. We determined that the results of blood typing using the LR-SPR biosensor are consistent with the results obtained from the agglutination test. We obtained the lowest detection limits of 1.58 × 105 cells/ml for RBC-A and 3.83 × 105 cells/ml for RBC-B, indicating that the LR-SPR chip has a higher sensitivity than conventional SPR biosensors (3.3 × 108 cells/ml). The surface of the biosensor can be efficiently regenerated using 20 mM NaOH. In summary, as the LR-SPR technique is sensitive and has a simple experimental setup, it can easily be applied for ABO blood group typing.

scholarly journals Optical Characterization of Ultra-Thin Films of Azo-Dye-Doped Polymers Using Ellipsometry and Surface Plasmon Resonance Spectroscopy

Photonics ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 41
Author(s):  
Najat Andam ◽  
Siham Refki ◽  
Hidekazu Ishitobi ◽  
Yasushi Inouye ◽  
Zouheir Sekkat
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Spectroscopic Ellipsometry ◽  
Plasmon Resonance ◽  
Complex Refractive Index ◽  
Resonance Spectroscopy ◽  
Doped Polymers

The determination of optical constants (i.e., real and imaginary parts of the complex refractive index (nc) and thickness (d)) of ultrathin films is often required in photonics. It may be done by using, for example, surface plasmon resonance (SPR) spectroscopy combined with either profilometry or atomic force microscopy (AFM). SPR yields the optical thickness (i.e., the product of nc and d) of the film, while profilometry and AFM yield its thickness, thereby allowing for the separate determination of nc and d. In this paper, we use SPR and profilometry to determine the complex refractive index of very thin (i.e., 58 nm) films of dye-doped polymers at different dye/polymer concentrations (a feature which constitutes the originality of this work), and we compare the SPR results with those obtained by using spectroscopic ellipsometry measurements performed on the same samples. To determine the optical properties of our film samples by ellipsometry, we used, for the theoretical fits to experimental data, Bruggeman’s effective medium model for the dye/polymer, assumed as a composite material, and the Lorentz model for dye absorption. We found an excellent agreement between the results obtained by SPR and ellipsometry, confirming that SPR is appropriate for measuring the optical properties of very thin coatings at a single light frequency, given that it is simpler in operation and data analysis than spectroscopic ellipsometry.

scholarly journals Numerical Study on the Surface Plasmon Resonance Tunability of Spherical and Non-Spherical Core-Shell Dimer Nanostructures

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1728
Author(s):  
Joshua Fernandes ◽  
Sangmo Kang
Keyword(s):  
Refractive Index ◽  
Optical Properties ◽  
Surface Plasmon Resonance ◽  
Aspect Ratio ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Shell Thickness ◽  
Numerical Study ◽  
Field Enhancement ◽  
Core Shell

The near-field enhancement and localized surface plasmon resonance (LSPR) on the core-shell noble metal nanostructure surfaces are widely studied for various biomedical applications. However, the study of the optical properties of new plasmonic non-spherical nanostructures is less explored. This numerical study quantifies the optical properties of spherical and non-spherical (prolate and oblate) dimer nanostructures by introducing finite element modelling in COMSOL Multiphysics. The surface plasmon resonance peaks of gold nanostructures should be understood and controlled for use in biological applications such as photothermal therapy and drug delivery. In this study, we find that non-spherical prolate and oblate gold dimers give excellent tunability in a wide range of biological windows. The electromagnetic field enhancement and surface plasmon resonance peak can be tuned by varying the aspect ratio of non-spherical nanostructures, the refractive index of the surrounding medium, shell thickness, and the distance of separation between nanostructures. The absorption spectra exhibit considerably greater dependency on the aspect ratio and refractive index than the shell thickness and separation distance. These results may be essential for applying the spherical and non-spherical nanostructures to various absorption-based applications.

scholarly journals Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors

Sensors ◽  
2021 ◽  
Vol 21 (13) ◽  
pp. 4348
Author(s):  
Piotr Mrozek ◽  
Ewa Gorodkiewicz ◽  
Paweł Falkowski ◽  
Bogusław Hościło
Keyword(s):  
Refractive Index ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Real Life ◽  
Reflectance Measurement ◽  
Near Surface ◽  
Bimetallic Surface ◽  
Calibration Curves ◽  
Surface Sensing

Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations.

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