Cytotoxic Activity of Ethyl-para-methoxycinnamate from Kaempferia galanga L. on A549 Lung Cancer and B16 Melanoma Cancer Cells

Kaempferia galanga L. belongs to the family of Zingiberaceae, an endangered medicinal plant with pharmacology activities. Ethyl-p-methoxycinnamate (EPMC) is an essential phytoconstituent of K. galanga rhizomes. Several studies have reported that EPMC has anticancer activities in several cancer cells, including CL-6 gallbladder cancer cells, HepG2liver cancer cells, MCF-7 breast cancer cells, and Raji lymphoma cancer cells. However, studies on A549 lung cancer and B16 melanoma cancer cells have not been reported. This study aimed to determine the anticancer activity of EPMC against A549 lung cancer and B16 melanoma cancer cells. EPMC was obtained by extraction using n-hexane, then recrystallized with chloroform. The isolate was then analyzed by thin-layer chromatography (TLC), and the structure was characterized by Fourier Transform Infrared (FTIR) and Nuclear Magnetic Resonance (NMR) spectroscopy. Cytotoxic activity was determined under Presto Blue assay. Based on the result, EPMC from K. galanga showed the cytotoxic effect on B16 cells with an IC50 value of 97.09 μg/mL, whereas EPMC showed no significant cytotoxic effect on A549 with an IC50 value of 1407.75 μg/mL. It was concluded that EPMC has potential cytotoxic on B16 melanoma cancer cells, but it showed inactive activity against A549 lung cancer cells. Further molecular mechanism underlying EPMC cytotoxic activity needs to be conducted.

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Cytotoxic Effect of Oleanolic Acid-Coated Magnetic Nanoparticles on Reversal of Multidrug Resistance in A549 Lung Cancer Cells

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2016.13284 ◽

2016 ◽

Vol 16 (10) ◽

pp. 11031-11034

Author(s):

Kwon-Jai Lee ◽

Jeung Min Lee ◽

Jae-Soo Shin ◽

Dong-Hee Kim ◽

Jeung Hee An

Keyword(s):

Lung Cancer ◽

Multidrug Resistance ◽

Magnetic Nanoparticles ◽

Cancer Cells ◽

Oleanolic Acid ◽

Cytotoxic Effect ◽

Lung Cancer Cells ◽

A549 Lung

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Evaluation of the Cytotoxic Effect of Rutin Prenanoemulsion in Lung and Colon Cancer Cell Lines

Journal of Nanomaterials ◽

10.1155/2020/8867669 ◽

2020 ◽

Vol 2020 ◽

pp. 1-11

Author(s):

Tran Thanh Hoai ◽

Phan Thi Yen ◽

Tran Thi Bich Dao ◽

Luu Hai Long ◽

Duong Xuan Anh ◽

...

Keyword(s):

Lung Cancer ◽

Colon Cancer ◽

Particle Size ◽

Cancer Cells ◽

Cancer Cell ◽

Cytotoxic Effect ◽

Chemical Structure ◽

Colon Cancer Cell ◽

In Vitro Tests ◽

A549 Lung

In this work, prenanoemulsion of rutin was prepared using PEG and Tween as emulsifiers via homogenization and evaporation techniques. The particle size of rutin was investigated with high-resolution transmission electron microscopy (HR-TEM); particle size distribution and its chemical structure were analysed by nuclear magnetic resonance (NMR) and Fourier transformed infrared (FT-IR) spectroscopy. It was found that rutin in the prenanoemulsion has excellent solubility in water with the size approximately 15 nm. The chemical structure of nanorutin in prenanoemulsion was identical to that of the pure rutin. It suggested that there is no chemical modification of rutin in the prenanoemulsion. From high-performance liquid chromatography (HPLC), the amount of rutin in the prenanoemulsion was determined to be 9.27%. The cytotoxic effect of rutin in the preemulsion was investigated via in vitro tests to determine rutin’s efficacy in A549 lung cancer cell and colon cancer cell treatment. The results demonstrated that rutin in the prenanoemulsion could inhibit A549 lung cancer cells and colon cancer cells efficiently.

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Anticancer Activities of Sesewanua Leaf Extracts (Clerodendrum fragrans (Vent.) Willd) Against A549 Lung Cancer Cell

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.7484 ◽

2021 ◽

Vol 9 (A) ◽

pp. 1226-1230

Author(s):

Elisabeth Natalia Barung ◽

Donald Emilio Kalonio ◽

Yos Banne ◽

Norma Tiku Kambuno

Keyword(s):

Lung Cancer ◽

Ethyl Acetate ◽

Anticancer Activity ◽

Cancer Cells ◽

Ethyl Acetate Fraction ◽

Water Soluble ◽

Lung Cancer Cells ◽

Assay Method ◽

Anticancer Activities ◽

A549 Lung

BACKGROUND: Cancer is one of the leading causes of non-communicable diseases in the world, with about 10 million deaths worldwide in 2020. Lung cancer was the most common type of cancer and the highest cause of death. Therapy for lung cancer can be either conventional therapy or molecular targeted therapy that has many limitations. AIM: It is, therefore, important to explore new sources of anticancer activity, including those from plants. One plant that is thought to have anticancer activity is Sesewanua (Clerodendrum fragrans [Vent.] Willd. Syn. Clerodendrum chinense [Osbeck] Mabb., Family Lamiaceae). METHODS: This research is a laboratory experiment. The sample used is the C. fragrans leaves obtained in Malalayang I Timur Village, Malalayang District, Manado City, North Sulawesi Province, while the subjects in this study were A549 lung cancer cells from Cell-Culture Laboratory, Faculty of Pharmacy, Universitas Padjadjaran Bandung. Anticancer activity test was using the MTT tetrazolium assay method. Data in the form of a percentage (%) inhibition of cell proliferation, then determined the value the concentration of 50% proliferation inhibition (IC50) using a computer program online. RESULTS: The results showed that ethanol extract, hexane fraction, ethyl acetate fraction, and water-soluble fraction of C. fragrans had anticancer activity on A549 lung cancer cells. The smallest IC50 value is indicated by ethyl acetate fraction (191, 165 ppm), which is categorized as moderately active.

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Indomethacin-Enhanced Anticancer Effect of Arsenic Trioxide in A549 Cell Line: Involvement of Apoptosis and Phospho-ERK and p38 MAPK Pathways

BioMed Research International ◽

10.1155/2013/237543 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 10

Author(s):

Ali Mandegary ◽

Maryam Torshabi ◽

Mohammad Seyedabadi ◽

Bagher Amirheidari ◽

Elham Sharif ◽

...

Keyword(s):

Lung Cancer ◽

Combination Therapy ◽

Cancer Cells ◽

Arsenic Trioxide ◽

Cytotoxic Effect ◽

Caspase 3 ◽

Mapk Pathway ◽

Lung Cancer Cells ◽

Gene And Protein Expression ◽

A549 Lung

Background. Focusing on novel drug combinations that target different pathways especially apoptosis and MAPK could be a rationale for combination therapy in successful treatment of lung cancer. Concurrent use of cyclooxygenase (COX) inhibitors with arsenic trioxide (ATO) might be a possible treatment option.Methods. Cytotoxicity of ATO, dexamethasone (Dex), celecoxib (Cel), and Indomethacin (Indo) individually or in combination was determined at 24, 48, and 72 hrs in A549 lung cancer cells. The COX-2 gene and protein expression, MAPK pathway proteins, and caspase-3 activity were studied for the most cytotoxic combinations.Results. The IC50s of ATO and Indo were 68.7 μmol/L and 396.5 μmol/L, respectively. Treatment of cells with combinations of clinically relevant concentrations of ATO and Indo resulted in greater growth inhibition and apoptosis induction than did either agent alone. Caspase-3 activity was considerably high in the presence of ATO and Indo but showed no difference in single or combination use. Phosphorylation of p38 and ERK1/2 was remarkable in the concurrent presence of both drugs.Conclusions. Combination therapy with ATO and Indo exerted a very potent in vitro cytotoxic effect against A549 lung cancer cells. Activation of ERK and p38 pathways might be the mechanism of higher cytotoxic effect of ATO-Indo combination.

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