Background:
Endophytic fungi live in plants’ tissue and can produce the same bioactive
compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of
the antibacterial compound producers.
Aim and Objective:
This study aimed to characterize morphologically, microscopically, and
molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves.
Methods:
The isolation of endophytic fungi was done by fragment planting method on PDA medium.
The antibacterial screening was performed using the antagonistic test as the first screening
followed by the disc diffusion test method. The morphological characterization was based on isolate’s
mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic
characterization was based on its hyphae characteristics. The molecular characterization of
the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS
rDNA genes of the isolate.
Results:
The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus,
P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation
extract on S.aureus with a diameter of inhibition of 13.77 mm.
Conclusion:
Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is
similar to Ceriporialacerate.
In vitro symbiotic seed germination and molecular characterization of associated endophytic fungi in a commercially important and endangered Indian orchid Vanda coerulea Griff. Ex Lindl.
