scholarly journals Effect of high protein diet in activity of antioxidants and level of inflammation on sprint athlete

2021 ◽  
Vol 57 (2) ◽  
pp. 185-194
Author(s):  
Endang Sri Wahjuni ◽  
Soetanto Hartono

This study aims to analyze the effect of a low-carbohydrate high-protein diet to increase the level of antioxidant, decrease inflammation and improve performance of athlete. The research was carried out by experimental research methods, within the design of “Randomized Control Group Pretest Posttest Design”. A sample was taken from 20 people who met the inclusion and exclusion criteria from 30 teenage athletes in PASI East Java sprints. The Data were collected by measuring the sprint results by finish photo camera and laboratory examinations to determine the levels of antioxidants (SOD) and the inflammation degree (TNF-α) in blood. The data were analyzed using multivariate technique (Manova) Hotelling’s method (T2). Hypothesis testing using α = 0.05. The results and conclusions of the study stated that the normal diet had no effect on the variables of sprint running speed, SOD and TNF-α levels. While low-carbohydrate and high-protein diet can increase SOD levels of 211.44/gHb, reduce (TNF-α) at least 0.309 pg/ml, and the average increase in antioxidant activity caused by low-carbohydrate-high-protein diet is 24.989/gHb higher than normal diet, the decrease in the degree of inflammation is 0.196 pg/ml, however, it has no effect on the speed of sprint.

2021 ◽  
Author(s):  
Endang Sri Wahjunia ◽  
Soetanto Hartono

This study aims to analyze the effect of a low-carbohydrate high-protein diet to increase the level of antioxidant, decrease inflammation and improve performance of athlete. The research was carried out by experimental research methods, within the design of "Randomized Control Group Pretest Posttest Design".A sample was taken from 20 people who met the inclusion and exclusion criteria from 30 teenage athletes in PASI East Java sprints. The Data were collected by measuring the sprint results by finish photo camera and laboratory examinations to determine the levels of antioxidants (SOD) and the inflammation degree (TNF α)in blood. The data were analyzed using multivariate technique (Manova) Hotelling's method (T2). Hypothesis testing using α0.05. The results and conclusions of the study stated that the normal diet had no effect on the variables of sprint running speed, SOD and TNF-α levels. While low-carbohydrate and high-protein diet can increase SOD levels of 211.44 /gHb, reduce (TNF α) at least 0.309 pg/ml, and the average increase in antioxidant activity caused by low-carbohydrate-high-protein diet is 24,989 / gHb higher than normal diet, the decrease in the degree of inflammation is 0.196 pg/ml, however, it has no effect on the speed of sprint.


2005 ◽  
Vol 4 (1) ◽  
Author(s):  
Donald Harris ◽  
Christopher Bell ◽  
Misty Retzlaff ◽  
Stephanie Toering ◽  
Elizabeth Wurdak ◽  
...  

This study was designed to investigate the long-term metabolic adaptations and health effects of a low-carbohydrate, high-fat/protein diet in mice. One-month-old male ICR mice were fed a control, conventional high-carbohydrate diet (n=21) or an experimental low-carbohydrate, high-fat, high-protein diet (n=20). One pair of mice per group was euthanized at two-week intervals for five months for tissue analysis. Basic metabolic data, body and tissue weights, blood and plasma metabolite and lipid profiles, liver glycogen and protein content, and liver serine dehydratase and glucose-6-phosphate dehydrogenase activities were analyzed. The low-carbohydrate group gained significantly more weight (p<0.005 after 4 weeks) than the normally growing control group. Although ketosis was initially stimulated in the low-carbohydrate group, enzyme and tissue analysis suggest that gluconeogenic activity was sufficient to alleviate the effects of severe dietary carbohydrate restriction and allow for glucose metabolism close to that demonstrated in the control group.


2021 ◽  
Vol 64 ◽  
pp. 258-264
Author(s):  
Debajyoti Bhattacharya ◽  
Prerona Biswas ◽  
Somnath Gangopadhyay ◽  
Mausumi Sikdar Bhakta

Objectives: Nowadays, mobile phones have become a prime necessity. At present, various advance networking connections such as 3G and 4G are available in the market to get a faster service. However, these mobile phones emit harmful electromagnetic radiations which have various ill effects on different physiological systems. The present study has been done to find out the extent of damage caused by mobile phone radiation on liver cells, and, whether high-protein diet (HPD) has any ameliorative effect on such changes. Materials and Methods: Male Swiss Albino mice were divided into four groups. Two groups of animals were exposed to mobile phone radiation at global system for mobile communications like frequencies at 1.8 GHz for 3 h continuously per day for 90 days. One of these radiation exposed groups received a normal diet (containing 5% casein) and the other exposed group received a HPD (supplemented with 20% casein). The control group animals received only a normal diet, while the animals of the remaining group received only a HPD. The serum biochemical and histological parameters of liver tissues and ladder assay of hepatic DNA were studied. Results: A significantly elevated level (P < 0.05) of aspartate aminotransferase, alanine transaminase, bilirubin and serum caspase-3 levels was observed in the group of animals exposed to electromagnetic radiation emitted from mobile phone. Histological changes were also observed in the experimental groups. Moreover, in genomic DNA ladder assay, fragmented DNA was observed in exposed group, which is a probable sign of cell apoptosis. Recovery symptoms were observed in the animals supplemented with high casein diet. Conclusion: From the present study, it may be concluded that electromagnetic radiation emitted from mobile phones has damaging effects on hepatocytes which may be ameliorated by HPD.


2004 ◽  
Vol 16 (2) ◽  
pp. 190 ◽  
Author(s):  
D.K. Gardner ◽  
K.S. Stilley ◽  
M. Lane

Ammonium is known to adversely affect the development of mouse embryos in culture. Specifically, ammonium has been found to impair inner cell (ICM) mass formation, increase apoptosis, retard fetal development following embryo transfer and induce exencephaly. Significantly, high protein diets in cattle lead to reduced fecundity. This has been linked to elevated urea levels within fluid of the female tract. In this study we have determined the effects of a high protein diet for mice on the levels of ammonium within the female tract and the effects of such a diet on the development and viability of blastocysts developed in vivo. Outbred mice (CF1) were fed a diet of either 25% (high protein) or 14% (control) protein for 4 weeks. Females were superovulated and mated to males of the same strain. In 24 mice, oviduct fluid was collected at 22h post hCG. Ammonium in the oviduct fluid was then quantitated fluorometrically. From other animals, blastocysts were flushed 92h post hCG and analyzed. Blastocyst differentiation and apoptotic indices were determined. Values are mean±SEM. Data were analysed using Student’s t-test. The levels of ammonium in the oviduct were significantly higher (P&lt;0.01) in females fed the high protein diet (356±43μM) compared to the control (68±13μM) (n=12 in each group). Blastocysts (n=139) from females fed the high protein diet had significantly lower total (43.4±1.1; P&lt;0.05) and ICM cell numbers (12.7±0.4; P&lt;0.01), compared to the control group (46.8±0.9 and 15.4±0.4 respectively; n=124). Furthermore, blastocysts from animals fed a high protein diet had a significantly higher apoptotic index (8.7±1.4; P&lt;0.01) compared to the control group (2.0±0.5). These data show that consumption of a high protein diet results in the excess accumulation of ammonium in the fluid of the female reproductive tract of mice. These high levels of ammonium subsequently impair the formation of the fetal progenitor cells and increase cell death at the blastocyst stage. These data from in vivo-developed mouse blastocysts are similar to those for blastocysts developed in culture in the presence of 300μM ammonium. Therefore, it is not advisable to maintain mice on a high protein diet. These data have significant implications for animal breeding, and for patients attempting IVF treatment.


1996 ◽  
Vol 80 (4) ◽  
pp. 1365-1370 ◽  
Author(s):  
O. L. Svendsen ◽  
M. Krotkiewski ◽  
C. Hassager ◽  
C. Christiansen

The main aim of this study was to investigate the effect of an energy-restrictive, high-protein diet with or without exercise on muscle morphology and biochemistry. Moderately overweight postmenopausal women (49-58 yr, body mass index: 25-42 kg/m2) were randomly assigned to three groups for 12 wk of intervention; namely, a control group, a group on a 4.2 MJ/day diet, and a group on 4.2 MJ/day diet combined with aerobic and anaerobic exercise. Muscle morphology and biochemistry analysis were performed in 69 and 58 women, respectively. In contrast to the diet-only group, the die-plus- exercise group significantly increased the muscle fiber areas by 20-25%, the number of capillaries per muscle fiber type I by approximately 20%, and the activities of citrate synthase by approximately 35% and hexokinase by approximately 20% (P < 0.05). There were no statistically significant changes in any other muscle variable (P < 0.05). The respiratory exchange ratio decreased in both intervention groups by 2-4% (P < 0.01). It is concluded that 12-wk period of an energy-restrictive high-protein diet was not associated with major changes in muscle morphology or biochemistry. The addition of exercise to the die led to an adaptive increase in muscle fiber areas and in the oxidative capacity of the muscles.


2011 ◽  
Vol 71 (13) ◽  
pp. 4484-4493 ◽  
Author(s):  
Victor W. Ho ◽  
Kelvin Leung ◽  
Anderson Hsu ◽  
Beryl Luk ◽  
June Lai ◽  
...  

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