scholarly journals Optimization of culture conditions for extracellular fungal lipase production by submerged fermentation process

2018 ◽  
Vol 5 (3) ◽  
pp. 135-141 ◽  
Author(s):  
. Shreya ◽  
Arun Kumar Sharma ◽  
Vinay Sharma ◽  
Jyoti Saxena
Keyword(s):  
Lipase Activity ◽  
Specific Activity ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Potassium Nitrate ◽  
Lipase Production ◽  
Culture Conditions ◽  
Mustard Oil ◽  
Malt Extract ◽  
Inorganic Nitrogen Source

The present study aimed to optimize culture conditions for optimal growth and production of extracellular lipase. Lipolytic fungal strain named as S3St2 previously isolated from a petrol pump soil sample of Newai Town was used for optimization study. Among the tested carbohydrate carbon sources, polysaccharide-starch exhibited maximum lipase production (21.25±0.70 IU/ml/min) with highest specific activity (1.47±0.06 U/mg). Lipase activity and specific activity were higher with mustard oil 1 % (v/v) among all lipidic carbon sources. Among inorganic nitrogen source, potassium nitrate was found best inducer of lipase activity, malt extract supported the fungus growth (dry weight of cell pellets was 0.467 g) and exhibited maximum lipase activity among all organic nitrogen sources. Lipase activity was optimum at pH 8.0, indicates alkalophillic nature of production media supports the growth of fungus. Higher lipase activity (27.92±0.87 IU/ml/min) was detected at 28ºC. The incubation time of 5 days was found optimum for maximum lipase production (31.51±0.21 IU/ml/min).

scholarly journals Agroresidues enhanced peroxidase activity expression by Bacillus sp. MABINYA-1 under submerged fermentation

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Ayodeji O. Falade ◽  
Leonard V. Mabinya ◽  
Anthony I. Okoh ◽  
Uchechukwu U. Nwodo
Keyword(s):  
Wheat Straw ◽  
Peroxidase Activity ◽  
Submerged Fermentation ◽  
Specific Activity ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Fold Increase ◽  
Kraft Lignin ◽  
Inorganic Nitrogen Source ◽  
Maize Stover

Abstract Agroresidues have continued to gain preference over conventional carbon sources for microbial enzyme production due to the low price and abundance in the environment. Therefore, this study aimed at improving peroxidase yield by Bacillus sp. MABINYA-1 (BMAB-1) using agroresidues under submerged fermentation. The culture parameters that support maximum peroxidase yield by BMAB-1 was initially determined and the results showed that peroxidase activity expression was optimum at pH 5, 30 °C and 150 rpm while veratryl alcohol and ammonium sulphate served as the best peroxidase-inducer and inorganic nitrogen source, respectively. BMAB-1 exhibited maximum peroxidase expression (17.50 ± 0.10 U/mg) at 72 h using kraft lignin liquid medium (KLLM) under the optimized culture conditions. Upon utilization of selected agroresidues (sawdust, wheat straw and maize stover) as sole carbon sources by BMAB-1 in the fermentation process, peroxidase activity was significantly enhanced when compared with glucose (14.91 ± 0.31 U/mg) and kraft lignin (17.50 ± 0.10 U/mg). Sawdust produced the highest peroxidase yield (47.14 ± 0.41 U/mg), followed by maize stover (37.09 ± 0.00 U/mg) while wheat straw yielded the lowest peroxidase specific activity (21.65 ± 0.35 U/mg). This indicates that utilization of sawdust by BMAB-1 resulted in 3.2- and 2.7-fold increase in peroxidase activity expression as compared to glucose and kraft lignin, respectively. The aptitude of BMAB-1 to utilize agroresidues would reduce the cost of peroxidase production by the bacteria since the substrates are cheaper than the conventional carbon sources and are, as well, more readily available.

scholarly journals Studies on the production of glucose isomerase by Bacillus licheniformis

2015 ◽  
Vol 17 (3) ◽  
pp. 84-88 ◽  
Author(s):  
Ogbonnaya Nwokoro
Keyword(s):  
Enzyme Activity ◽  
Bacillus Licheniformis ◽  
Organic Nitrogen ◽  
Specific Activity ◽  
Inorganic Nitrogen ◽  
Glucose Isomerase ◽  
Culture Conditions ◽  
Enzyme Productivity ◽  
Carbon Substrates ◽  
Optimum Ph

Abstract This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis. The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1% glycerol (specific activity = 6.88 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity = 5.24 U/mg protein). Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Best enzyme activity was observed in the presence of Mg2+ (specific activity = 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity = 1.02 U/mg protein). The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50ºC.

The lipase activity of Talaromyces emersonii

1978 ◽  
Vol 56 (16) ◽  
pp. 1840-1843 ◽  
Author(s):  
B. A. Oso
Keyword(s):  
Liquid Medium ◽  
Yeast Extract ◽  
Lipase Activity ◽  
Natural Habitat ◽  
Carbon Sources ◽  
Lipase Production ◽  
Extracellular Lipase ◽  
Talaromyces Emersonii ◽  
Stationary Liquid ◽  
Presence And Absence

Studies were carried out on the ability of Talaromyces emersonii Stolk to produce extracellular lipase in stationary liquid medium under various conditions. The best temperatures for lipase synthesis and activity were 40–45 °C, and at all the temperatures (37–55 °C) tested for lipase production, maximum enzyme was produced 8 days after incubation. Lipase synthesis was induced when various carbohydrates were used as carbon sources both in the presence and absence of yeast extract. The significance of lipase production in relation to the natural habitat is discussed.

scholarly journals Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

2018 ◽  
Vol 44 (2) ◽  
pp. 285-292
Author(s):  
Sereen Gul ◽  
Mujeeb Ur Rahman ◽  
Mohammad Ajmal ◽  
Abdul Kabir Khan Achakzai ◽  
Asim Iqbal
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Nitrate ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Neutral Protease ◽  
Carbon And Nitrogen Sources ◽  
Inorganic Nitrogen Source ◽  
Carbon And Nitrogen

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

Growth, nutrition, and proteolytic activity of the sap-staining fungus Ophiostoma piceae

1993 ◽  
Vol 71 (9) ◽  
pp. 1224-1230 ◽  
Author(s):  
L. D. Abraham ◽  
A. Roth ◽  
J. N. Saddler ◽  
C. Breuil
Keyword(s):  
Nitrogen Source ◽  
Proteolytic Activity ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Minimal Growth ◽  
Inorganic Nitrogen Source ◽  
Ophiostoma Piceae

The sap-staining ascomycete Ophiostoma piceae strain 387N used ammonium, but not nitrate, as an inorganic nitrogen source. Organic nitrogen sources assimilated included bovine serum albumin, collagen, acid-hydrolyzed casein, urea, and various amino acids. Sucrose, glucose, maltose, raffinose, and soluble starch were suitable carbon sources. The optimum temperature for growth was near 23 °C, with an upper limit at 35 °C and minimal growth at 4 °C after 3 days. An initial pH of 6.1 yielded the greatest biomass. Proteolytic activity was greatest in cultures supplemented with protein as the nitrogen source, but some activity was detected in cultures with no assimilable source of nitrogen. Proteinases were detected throughout growth in protein-supplemented liquid media, and they appeared to hydrolyze azocoll, with optimal activity at pH 8. Isoelectric focusing gels of culture filtrates, obtained after fungal growth on protein supplemented media, showed a major proteolytic band focusing at pH 5.2. Key words: staining fungi, Ophiostoma, nutrition, biomass, proteinases.

scholarly journals Effect of inoculum size, inducer and metal ion on lipase production by Rhodococcus strain UCC 0009

2020 ◽  
Vol 211 ◽  
pp. 02012
Author(s):  
Maegala Nallapan Maniyam ◽  
Anupriya Sundarajoo ◽  
Hazeeq Hazwan Azman ◽  
Nor Suhaila Yaacob ◽  
Hasdianty Abdullah
Keyword(s):  
Lipase Activity ◽  
Metal Ion ◽  
Optimization Method ◽  
Enzyme Characterization ◽  
Lipase Production ◽  
Industrial Applications ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Local Resources ◽  
The One

Lipases are critical enzymes for industrial applications such as in the food and pharmaceutical fields. Therefore, the discovery of new lipases with enhanced characteristics are always encouraged. Thus, the present study explored the ability of a novel bacterial strain isolated from a tropical climate for lipase production. The optimization method using the one-variable-at-a-time approach was adopted to obtain increased production of lipase. The strain identified as Rhodococcus strain UCC 0009 was able to generate specific lipase activity of 11.67a ± 0.00 mU/mg at optimized conditions of 8 % (v/v) inoculum concentration, 1 % (v/v) olive oil as the inducer, and the addition of Ca2+ions. The specific lipase activity increased by 162 % when the optimization using a one-variable-ata-time approach was adopted compared to that of the non-optimized counterpart, signifying this experimental phase’s importance. The present study’s findings revealed the potential of utilizing Rhodococcus strain UCC 0009 as a green lipase producer for application in bioremediation and biotransformation at an industrial scale. Further study concentrating on enzyme characterization and improving culture conditions for conducive production of lipase via statistical optimization using response surface methodology (RSM) will be attempted to elucidate further the superiority of lipase obtained from local resources.

scholarly journals Optimum vegetative growth conditions and genetic diversity in different strains of Pleurotus salmoneastramineus L.J.N. Vassilzeva

2020 ◽  
Vol 49 (1) ◽  
pp. 125-134
Author(s):  
Nuhu Alam ◽  
Farhana Rahman
Keyword(s):  
Genetic Diversity ◽  
Mycelial Growth ◽  
Culture Media ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Its Region ◽  
Growth Conditions ◽  
Malt Extract ◽  
Arbitrary Primers

This experiment was undertaken to depict the favourable condition for mycelial growth, molecular identification and phylogenetic relationship of the selected strains of Pleurotus salmoneostramineus. Suitable temperature and pH were obtained at 25ºC and 6, respectively. Mushroom complete, glucose peptone and yeast malt extract culture media were favorable, while Hennerberg and Hoppkins were unfavorable. Dextrin was the best and xylose was the less effective carbon sources. Inorganic nitrogen sources were less effective for the mycelial growth of P. salmoneostramineus. The sequences of internal transcribed spacer (ITS) region of selected strains revealed that the total length ranged from 614 to 663 bp. The size of the ITS1 and ITS2 regions varied among the strains. Sequence analysis showed that 5.8S of rDNA sequences were identical. Phylogenetic tree of the ITS region sequences indicated that strains of P. salmoneostramineus belong to same cluster. The reciprocal homologies of the ITS region sequences ranged from 98 to 100%. The strains of P. salmoneostramineus were also analyzed by random amplification of polymorphic DNA (RAPD) with 20 arbitrary primers. RAPD results suggested that tested strains of P. salmoneostramineus were genetically similar with some variations, thus it could be concluded that RAPD and ITS techniques were well competent for detecting the genetic diversity of all tested strains of P. salmoneostramineus.

scholarly journals OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI

2015 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Armaini ◽  
Abdi Dharma ◽  
Sumaryati Syukur ◽  
Jamsari
Keyword(s):  
Nitrogen Source ◽  
Hot Springs ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Optimum Concentration ◽  
Crystalline Cellulose ◽  
Inorganic Nitrogen Source

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources

scholarly journals Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 781-786 ◽  
Author(s):  
Maximo Sánchez ◽  
Martha-Helena Ramírez-Bahena ◽  
Alvaro Peix ◽  
María J. Lorite ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Lotus Corniculatus ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).

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