scholarly journals Effects of carbon and nitrogen sources on production of proteases by Bacillus subtilis IC-5

2018 ◽  
Vol 44 (2) ◽  
pp. 285-292
Author(s):  
Sereen Gul ◽  
Mujeeb Ur Rahman ◽  
Mohammad Ajmal ◽  
Abdul Kabir Khan Achakzai ◽  
Asim Iqbal
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Nitrate ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Neutral Protease ◽  
Carbon And Nitrogen Sources ◽  
Inorganic Nitrogen Source ◽  
Carbon And Nitrogen

The effects of various carbon and nitrogen sources were evaluated on production of proteases by Bacillus subtilis IC-5. Both type and concentration of carbon and nitrogen sources influenced the production of proteases. Among the carbon sources glucose was found to be the most effective. It gave maximum production at 2% w/v concentration i.e., 1875 and 950 U/ml, alkaline and neutral protease, respectively. The response of Bacillus subtilis IC-5 towards synthesis and excretion of enzymes varied with the type of nitrogen sources. The addition of organic nitrogen sources to basal medium repressed the synthesis of proteases while the addition of inorganic nitrogen source such as sodium nitrate was found to be the best stimulating for alkaline and neutral protease synthesis. Sodium nitrate enhanced the production up to 62.40 and 10.52% of alkaline and neutral protease, respectively against w.r.t. control.

scholarly journals Nutritional Requirements for the Improvement of Growth and Sporulation of Several Strains ofMonascus purpureuson Solid State Cultivation

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Zahra Ajdari ◽  
Afshin Ebrahimpour ◽  
Musaalbakri Abdul Manan ◽  
Muhajir Hamid ◽  
Rosfarizan Mohamad ◽  
...  
Keyword(s):  
Solid State ◽  
Medium Optimization ◽  
Cell Mass ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Nutritional Requirements ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
The Media ◽  
Sporulation Rate

This paper describes the nutritional requirements for the improvement of growth and sporulation of several strains ofMonascus purpureuson solid state cultivation. The findings revealed that glucose enhanced growth of allM. purpureusstrains tested but inhibited the sporulation rate. On the other hand, sucrose induced sporulation but inhibited production of cell mass. A combination of glucose and sucrose greatly enhanced sporulation and cell mass production ofM. purpureus. Although growth and sporulation rate were related to the ratio of carbon to nitrogen (C/N ratio), the types and concentrations of carbon and nitrogen sources also greatly influenced the growth kinetics. Among the media tested, Hiroi-PDA medium was the most preferred medium for allM. purpureusstrains tested for the enhancement of radial growth rate, sporulation, and cell production. Hence, Hiroi-PDA could be suggested as the generic basal medium for the cultivation ofM. purpureus. However, individual medium optimization is required for significant enhancement in growth and sporulation of each strain ofM. purpureus.

Growth, nutrition, and proteolytic activity of the sap-staining fungus Ophiostoma piceae

1993 ◽  
Vol 71 (9) ◽  
pp. 1224-1230 ◽  
Author(s):  
L. D. Abraham ◽  
A. Roth ◽  
J. N. Saddler ◽  
C. Breuil
Keyword(s):  
Nitrogen Source ◽  
Proteolytic Activity ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Fungal Growth ◽  
Nitrogen Sources ◽  
Soluble Starch ◽  
Minimal Growth ◽  
Inorganic Nitrogen Source ◽  
Ophiostoma Piceae

The sap-staining ascomycete Ophiostoma piceae strain 387N used ammonium, but not nitrate, as an inorganic nitrogen source. Organic nitrogen sources assimilated included bovine serum albumin, collagen, acid-hydrolyzed casein, urea, and various amino acids. Sucrose, glucose, maltose, raffinose, and soluble starch were suitable carbon sources. The optimum temperature for growth was near 23 °C, with an upper limit at 35 °C and minimal growth at 4 °C after 3 days. An initial pH of 6.1 yielded the greatest biomass. Proteolytic activity was greatest in cultures supplemented with protein as the nitrogen source, but some activity was detected in cultures with no assimilable source of nitrogen. Proteinases were detected throughout growth in protein-supplemented liquid media, and they appeared to hydrolyze azocoll, with optimal activity at pH 8. Isoelectric focusing gels of culture filtrates, obtained after fungal growth on protein supplemented media, showed a major proteolytic band focusing at pH 5.2. Key words: staining fungi, Ophiostoma, nutrition, biomass, proteinases.

scholarly journals Caseinase Production and Media Optimization from Bacillus subtilis

2020 ◽  
Vol 71 (11) ◽  
pp. 1-9
Author(s):  
Noor Nihad Abdul Hussein ◽  
Aseel I. Ibrahim ◽  
Firas Hashim Kamar ◽  
Arelia Cristina Nechifor
Keyword(s):  
Bacillus Subtilis ◽  
Enzyme Production ◽  
Paper Industry ◽  
Nitrogen Sources ◽  
The Body ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Sequencing Result

Caseinase is involved in the breakdown of milk protein casein and converts casein into smaller simple sugars which can be easily utilized by the body for the production of ATP and Fat. Casein can be an instant energy source to the body and involves in muscle building. Caseinase enzyme can be extensively used at the industrial scale for Milk, Textile, Dairy, Paper industry and several other medical purposes. In view of the importance of caseinase, the current research deals with the isolation and identification of caseinase producing bacteria from soil. This is followed by the production of enzyme and its purification. The study also includes its kinetic characterization using the parameters Temperature, pH as well as Carbon and Nitrogen Sources. The organism which was isolated from soil and capable of producing the caseinase enzyme was identified to be Bacillus subtilis based on the Biochemical tests and 16S rRNA sequencing result. The optimal carbon and nitrogen sources were identified to be Glucose and casein respectively. Regarding the optimal conditions, the suitable temperature for maximum enzyme production was found to be 40 0C and pH was 9. When the organism was cultured under the optimal condition using casein as a nitrogen source and glucose as the carbon source, at 40 0C and pH 9, 1590 ng/mL of enzyme production was estimated.

scholarly journals Characterization of Diazotrophic Rhizobacteria under Various Conditions

2013 ◽  
Vol 1 (3) ◽  
pp. 110-117
Author(s):  
Umesh Prasad Shrivastava
Keyword(s):  
Nitrogenase Activity ◽  
Nifh Gene ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Accession Number ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Reduction Assay ◽  
Assay Methods

Nitrogenase activity was analysed after supplementation of various carbon and nitrogen sources in the growth medium by Acetylene Reduction Assay methods in selected 9 isolates from 74 diazotrophic isolates.  Enhancement in nitrogenase activity was recorded many fold by the addition of different organic carbon sources in which maltose and pyruvate showed better result than others. In case of supplementation of nitrogen sources, reduction of nitrogenase activity was observed.  Nitrogenase activity increased from 22.7 to 72.7% in various strains when they are tested in anaerobic condition, Amplification of fragment of 390 bp showed that nitrogenase activity due to presence of nifH gene.  Sequences were submitted to NCBI GeneBank and the accession number of nifH sequence of ECI-10A (FJ032023), AF-4B (FJ032024), AF-4C (FJ032022) and BN-2A (FJ032021) has been obtained. Phylogenetic analysis based on showed that these 4 isolates belong to the member of γ-proteobacteria, but show appreciable genetic diversity.DOI: http://dx.doi.org/10.3126/ijasbt.v1i3.8607 Int J Appl Sci Biotechnol, Vol. 1(3) 2013 : 110-117

scholarly journals Nutritional influences on Volvariella volvacea (Bull. ex. Fr.) Sing. growth in Puerto Rico. I. Carbon and nitrogen

1969 ◽  
Vol 72 (1) ◽  
pp. 19-29
Author(s):  
Ramón I. Torres ◽  
Paul R. Hepperly
Keyword(s):  
Sodium Nitrate ◽  
Organic Nitrogen ◽  
C:N Ratio ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Volvariella Volvacea ◽  
Carbon And Nitrogen ◽  
Straw Mushroom ◽  
Nutritional Influences ◽  
Vigorous Growth

Laboratory tests were conducted to determine the growth of a Puerto Rican strain of the straw mushroom Volvariella volvacea on diverse carbon and nitrogen sources at variable carbon/nitrogen (c:n) ratios. Of the carbon sources (cornstarch, cellulose, maltose and lactose), cornstarch and cellulose supported the most vigorous growth. No growth was observed on lactose and maltose. Urea, sodium nitrate, peptone and casein were tested as nitrogen sources at C:N ratios of ∞:1, 60:1, 30:1 and 15:1 with cornstarch as the sole carbon source. The 60:1 C:N ratio stimulated faster growth. Nitrogen sources urea and sodium nitrate did not support growth. Organic nitrogen sources, casein and peptone, stimulated the growth of V. volvacea, with the faster growth on casein.

scholarly journals Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

2014 ◽  
Vol 2014 ◽  
pp. 1-14 ◽  
Author(s):  
F. A. G. Gonçalves ◽  
G. Colen ◽  
J. A. Takahashi
Keyword(s):  
Carbon Sources ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Biotechnological Potential ◽  
Carbon And Nitrogen ◽  
Intracellular Lipids ◽  
Metabolites Production ◽  
Growing Conditions ◽  
Fermentation Parameters

Yarrowia lipolyticais a nonpathogenic dimorphic aerobic yeast that stands out due to its ability to grow in hydrophobic environments. This property allowed this yeast to develop an ability to metabolize triglycerides and fatty acids as carbon sources. This feature enables using this species in the bioremediation of environments contaminated with oil spill. In addition,Y. lipolyticahas been calling the interest of researchers due to its huge biotechnological potential, associated with the production of several types of metabolites, such as bio-surfactants,γ-decalactone, citric acid, and intracellular lipids and lipase. The production of a metabolite rather than another is influenced by the growing conditions to whichY. lipolyticais subjected. The choice of carbon and nitrogen sources to be used, as well as their concentrations in the growth medium, and the careful determination of fermentation parameters, pH, temperature, and agitation (oxygenation), are essential for efficient metabolites production. This review discusses the biotechnological potential ofY. lipolyticaand the best growing conditions for production of some metabolites of biotechnological interest.

THE EFFECT OF NUTRIENTS AND ENVIRONMENT ON SYNNEMATA FORMATION OF STILBELLA THERMOPHILA

1967 ◽  
Vol 13 (4) ◽  
pp. 351-360 ◽  
Author(s):  
K. K. Al-Hassan ◽  
C. L. Fergus
Keyword(s):  
Mycelial Growth ◽  
Nutrient Concentration ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Carbon Nitrogen Ratio ◽  
The Media ◽  
Nitrogen Ratio ◽  
Exogenous Supply

Stilbella thermophila Fergus was grown on a chemically denned medium of D-glucose, KNO3, MgSO4, KH2PO4, agar, and microelements to determine the effect of environment and nutrition on growth and synnemata production. An exogenous supply of thiamine stimulated growth markedly on pyridine-purified agar, but both thiamine and biotin were required for synnemata to form. Pyrimidine was the effective moiety, not thiazole. S. thermophila grew on a large number of carbon and nitrogen compounds substituted singly into the basal medium, but synnemata formed on less than half of such media. Sucrose did not inhibit synnemata formation with glucose present, but no synnemata formed with sucrose in the medium even with a number of different nitrogen sources. Synnemata production followed chance mold contamination on a few of the media that normally did not allow their production. The carbon–nitrogen ratio significantly affected synnemata formation. So did pH, concentration of phosphate buffer, and temperature. At suboptimal temperatures, reduced synnemata, or only loose bundles of conidiophores, formed. Light was not required for synnemata initiation nor for maturation. Synnemata formation occurred over a narrower range of temperature, pH, vitamin concentration, nutrient concentration, and nutrient spectrum (carbon and nitrogen sources) than did mycelial growth.

scholarly journals Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113

2016 ◽  
Vol 3 (1) ◽  
pp. 69-74
Author(s):  
Simeon Gavrailov ◽  
Viara Ivanova
Keyword(s):  
Nitrogen Source ◽  
Organic Nitrogen ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Casein Hydrolysate ◽  
Jerusalem Artichoke ◽  
Nitrogen Sources ◽  
Cellular Growth ◽  
Bacillus Sp ◽  
Carbon And Nitrogen

Abstract The effects of the carbon and nitrogen substrates on the growth of Bacillus sp. SG113 strain were studied. The use of organic nitrogen sources (peptone, beef extract, yeast extract, casein) leads to rapid cellular growth and the best results for the Bacillus strain were obtained with casein hydrolysate. From the inorganic nitrogen sources studied, the (NH4) 2SO4 proved to be the best nitrogen source. Casein hydrolysate and (NH4) 2SO4 stimulated the invertase synthesis. In the presence of Jerusalem artichoke, onion and garlic extracts as carbon sources the strain synthesized from 6 to 10 times more inulinase.

PROTEINASE BIOSYNTHESIS BY STREPTOCOCCUS LIQUEFACIENS: I. THE EFFECT OF CARBON AND NITROGEN SOURCES, pH, AND INHIBITORS

1956 ◽  
Vol 2 (7) ◽  
pp. 747-756 ◽  
Author(s):  
Robert Rabin ◽  
Leonard N. Zimmerman
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Sodium Fluoride ◽  
Basal Medium ◽  
Nitrogen Sources ◽  
Essential Amino Acids ◽  
High Concentration ◽  
Proliferating Cells ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Some nutritive aspects of proteinase biosynthesis by non-proliferating cells of Streptococcus liquefaciens, strain 31, were investigated by substituting constituents in a basal medium containing casein, lactose, purines, pyrimidines, vitamins, and salts. The casein of the medium could be replaced by a mixture of 12 "essential" amino acids (glutamic acid, histidine, valine, serine, methionine, leucine, isoleucine, arginine, cystine, lysine, tryptophane, and threonine), thus demonstrating that proteinase synthesis can occur in a medium devoid of protein. Proteinase biosynthesis appeared to depend upon an inordinately high concentration of arginine, required a fermentable carbohydrate, and occurred optimally at pH 6.3. Sodium fluoride and iodoacetate did not inhibit the proteinase activity but radically curbed its synthesis.

scholarly journals Protease Production from UV Mutated Bacillus subtilis

2012 ◽  
Vol 47 (1) ◽  
pp. 69-76 ◽  
Author(s):  
MG Sher ◽  
M Nadeem ◽  
Q Syed ◽  
M Irfan ◽  
S Baig
Keyword(s):  
Bacillus Subtilis ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Fermentation Medium ◽  
Maximum Activity ◽  
Inoculum Size ◽  
Protease Production ◽  
Protease Enzyme ◽  
Uv Mutation

UV mutation of the strain has significant contributation to enhance the yield of protease enzyme from Bacillus subtilis bacteria under the  cultivation conditions in submerged fermentation. The fermentation medium used for the production of protease composed of carbon  sources 1%, organic 1% or inorganic nitrogen sources 0.5% , K2HPO4 0.2 %, CaCl2 0.04% and MgSO4 0.02 % by mutated Bacillus subtilis  G-4 under the optimum parameters which are important to induce the mutated strain to produce high units of the protease, which were  temperature 37.5°C, pH 9, inoculum size 3 % v/v, glucose 1% as carbon source and peptone 1% as nitrogen source were give the maximum  455.25 + 1.66 units of protease. The results of stability studies revealed that protease of B. subtilis G-4 was stable over a broad range  of temperature (30 to 60°C) and pH (8 to 12). However, maximum activity (155.45U/ml) was observed at temperature 50°C and pH 10.  These characteristics render its potential use in detergent industries for detergent formulation.DOI: http://dx.doi.org/10.3329/bjsir.v47i1.10725 Bangladesh J. Sci. Ind. Res. 47(1), 69-76, 2012

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