scholarly journals Assessment of total phenolic content and antioxidant potentiality of selected Indian folk medicinal plants by spectrophotometric method

2020 ◽  
Vol 7 (3) ◽  
pp. 383-390
Author(s):  
Satish Dubey ◽  
Kundan Ojha ◽  
Jagriti Chandrakar ◽  
Rashmi Dehariya ◽  
Shilpa Vinodia ◽  
...  
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Spectrophotometric Method ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Dry Weight ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Leaf Extracts

Natural antioxidant capacity of five important folk medicinal plants measured in vitro. Total phenolic content (TPC), flavonoid content (FC) and free radical scavenging capacity of ethanolic, methanolic and aqueous leaf extracts of Lippia alba (LA), Annona squamosa (AS), Hyptis suaveolens (HS), Commiphora wightii (CW) and Milletia pinnata (MP) was assessed using spectrophotometric method. Folin ciocalteu and aluminium chloride method employed to optimise TPC and FC. Free radical scavenging potentiality of leaf extracts was assessed using Ferrous ion chelation (FIC), 2, 2'-diphenyl-1-picrylhydrazyl (DPPH•) scavenging, Hydroxyl (OH•) radical scavenging (HRS) and Superoxide (O- 2) radical scavenging (SRS) methods. Results revealed that the TPC (96.22±5.85 to 519.23±34.90 ?g GAE/gm dry weight) were found significant in aqueous extracts from all the plants except AS (p<0.05). For FC (?g QCE/gm dry weight), ethanol was found optimum for LA (463.94±6.49), CW (289.99±2.70) and MP (347.47±4.50) whereas, aqueous was found more appropriate for rest two plants were found significant instead of ethanol and methanol (all p<0.05). The lowest IC50 (?g/ml) were recorded from A. squamosa (27.72±8.95), H. suaveolens (27.78±0.88), C. wightii (27.18±0.16) and M. pinnata (27.30±0.03). All plants have reflected a high antioxidant capacity; however, the highest antioxidant activity was reported from ethanolic extract of H. suaveolens followed by L. alba, A. squamosa, C. wightii and M. pinnata. Hence, these studies show that all folk medicinal plants contain potential antioxidant bioactive compounds.

scholarly journals TOTAL PHENOLIC CONTENT AND FREE RADICAL SCAVENGING ACTIVITY OF MIMUSOPS ELENGI L. LEAVES

2019 ◽  
pp. 297-299
Author(s):  
VINEETA SINGH ◽  
PANDEY VN
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Leaf Extracts

Objective: The motive of the current study was to find out the total phenolic content (TPC) and free radical scavenging (FRS) activity of various solvent extracts of leaves. Methods: Leaves powder was subjected to extraction with different solvents, namely methanol, methanol 80%, ethanol, ethanol 80%, chloroform, acetone, aqueous, and ethyl acetate. Different concentrations of extracts were evaluated for FRS activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and TPC spectrophotometrically by Folin–Ciocalteu method. Results: The results clearly indicate that inhibition concentration of DPPH by leaf extracts was higher in methanol (inhibitory concentration 50=8.74) followed by ethanol >ethanol 80% >methanol 80% > acetone > aqueous > chloroform > ethyl acetate. TPC was higher in methanol (6.2±0.01 mg gallic acid equivalent/g) followed by ethanol > methanol 80% > acetone > aqueous >ethyl acetate > ethanol 80% > chloroform. A significant correlation (y=0.6265x +42.91 R2=0.9756) exists between TPC and FRS activity of methanolic extract. Conclusion: The finding revealed that methanolic extract of leaves contains a significant amount of FRS activity and phenolic compounds compared to other extracts.

scholarly journals Antioxidant Rich Extracts of Terminalia ferdinandiana Inhibit the Growth of Foodborne Bacteria

Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 281 ◽  
Author(s):  
Saleha Akter ◽  
Michael E. Netzel ◽  
Ujang Tinggi ◽  
Simone A. Osborne ◽  
Mary T. Fletcher ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Food Preservation ◽  
Total Phenolic ◽  
Foodborne Bacteria ◽  
Terminalia Ferdinandiana

Terminalia ferdinandiana (Kakadu plum) is a native Australian plant containing phytochemicals with antioxidant capacity. In the search for alternatives to synthetic preservatives, antioxidants from plants and herbs are increasingly being investigated for the preservation of food. In this study, extracts were prepared from Terminalia ferdinandiana fruit, leaves, seedcoats, and bark using different solvents. Hydrolysable and condensed tannin contents in the extracts were determined, as well as antioxidant capacity, by measuring the total phenolic content (TPC) and free radical scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. Total phenolic content was higher in the fruits and barks with methanol extracts, containing the highest TPC, hydrolysable tannins, and DPPH-free radical scavenging capacity (12.2 ± 2.8 g/100 g dry weight (DW), 55 ± 2 mg/100 g DW, and 93% respectively). Saponins and condensed tannins were highest in bark extracts (7.0 ± 0.2 and 6.5 ± 0.7 g/100 g DW). The antimicrobial activity of extracts from fruit and leaves showed larger zones of inhibition, compared to seedcoats and barks, against the foodborne bacteria Listeria monocytogenes, Bacillus cereus, Methicillin resistant Staphylococcus aureus, and clinical isolates of Pseudomonas aeruginosa. The minimum inhibitory concentration and minimum bactericidal concentration in response to the different extracts ranged from 1.0 to 3.0 mg/mL. Scanning electron microscopy images of the treated bacteria showed morphological changes, leading to cell death. These results suggest that antioxidant rich extracts of Terminalia ferdinandiana fruits and leaves have potential applications as natural antimicrobials in food preservation.

Effect of 1-MCP on Bioavailability of Lingwu Long Jujube In Vitro Digestion

2012 ◽  
Vol 554-556 ◽  
pp. 1357-1360
Author(s):  
Zhong Li Jiang ◽  
Ai Li Wang ◽  
Xi Hong Li ◽  
Min Peng Zhu ◽  
Jun Wei Wang
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Control Samples

The present study investigated the effect of 1-MCP on bioavailability of Lingwu long jujube stored at 0 °C for 60 days. At the end of the storage, compared with the control samples, Lingwu long jujube treated with 1-MCP exhibited higher Vc bioaccessibility, total phenolic content and free radical scavenging capacity, which demonstrated that 1-MCP treatment could provide a better effect on maintaining the bioavailability of Lingwu long jujube.

scholarly journals Antioxidant, Cytotoxic Activities and Total Phenolic Content of Four Indonesian Medicinal Plants

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Waras Nurcholis ◽  
Bambang Pontjo Priosoeryanto ◽  
Edy Djauhari Purwakusumah ◽  
Takeshi Katayama ◽  
Toshisada Suzuki
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Ic50 Values ◽  
Ethanol Extracts

The crude ethanol extracts of four Indonesian medicinal plants namely Curcuma xanthorrhiza Roxb.,Phyllanthus niruri Linn., Andrographis paniculata Ness., and Curcuma aeruginosa Roxb. wereexamined for their antioxidant (radical scavenging) activity using 2, 2-diphenyl-2-picrylhydrazyl(DPPH) free radical and cytotoxicity using brine shrimp lethality test (BSLT). The total phenoliccontent was used the Folin-Ciocalteu method. IC50 values for DPPH radical scavenging activityranged from 14.5 to 178.5 μg/ml, with P. niruri having the lowest value and therefore the mostpotent, and C. aeruginosa having the highest value. LC50 values for BSLT ranged from 210.3 to593.2 μg/ml, with C. xanthorrhiza and A. paniculata having the lowest and highest values,respectively. The total phenolic content of the Indonesian plants ranged from 133.0 ±3.7 to863.3±54.7 mg tannic acid equivalent per 1 g extract, with C. aeruginosa and P. niruri having thelowest and highest values, respectively. A positive correlation between free radical scavengingactivity and the content of phenolic compounds was found in the four of Indonesian medicinal plants.

Phytochemical and antioxidant activity of Cadaba farinosa Forssk stem bark extracts

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Emmanuel M. Halilu ◽  
Abdullahi M. Abdurrahman ◽  
Sylvester N. Mathias ◽  
Chinenye J. Ugwah-Oguejiofor ◽  
Muntaka Abdulrahman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Total Phenolic

Abstract Cadaba farinosa is used in traditional medicine for treatment of cancer, diabetes, and rheumatism. The research was aimed at evaluating the phytochemical and antioxidant activity of the extracts. The powdered stem bark was extracted successively with the aid of Soxhlet extractor using n-hexane, ethyl acetate, and methanol. The resulting extracts were concentrated on rotary evaporator and the percentage yields were calculated. The phytochemical and TLC profiles of the extracts were studied. The antioxidant activity of the extracts and ascorbic acid (standard) were determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The total phenolic content of the extracts and tannic acid (standard) were evaluated using Folin–Ciocalteu reagent. The percentage yields of n-hexane, ethyl acetate, and methanol extracts were found to be 1.19, 1.37, and 13.93%, respectively. The phytochemical screening revealed the presence of tannins, flavonoids, saponins, cardiac glycosides, alkaloids, and triterpenoids. The TLC profiles of the extracts revealed the presence of compounds as evidenced from their R f values. The total phenolic content of ethyl acetate and methanol extracts were found to be 135 and 112 mg, respectively. The free radical scavenging activity demonstrated by the extracts was comparable to ascorbic acid. The ethyl acetate extract had higher phenolic content and demonstrated the highest free radical scavenging with IC50 31.07 mg/mL. The results of research have provided strong preliminary evidence of antioxidant activity which may be because of phenolic compounds in the extracts.

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