scholarly journals Plant growth promoting bacteria Bacillus subtilis promote growth and physiological parameters of Zingiber officinale Roscoe

2021 ◽  
Vol 8 (1) ◽  
pp. 66-71
Author(s):  
D Jabborova ◽  
Y Enakiev ◽  
Khurshid Sulaymanov ◽  
Dilbar Kadirova ◽  
Ahmad Ali ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Height ◽  
Zingiber Officinale ◽  
Leaf Length ◽  
Leaf Width ◽  
Zingiber Officinale Roscoe ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting

Ginger (Zingiber officinale Roscoe) is an important medicinal crop grown for its aromatic rhizome which is used as a spice, food, flavouring agent and medicine. It has been characterised for its hypoglycemic, hypotensive, antioxidant and antibiotic properties. This study was conducted to determine the impact of plant growth-promoting potential of bacterial strain Bacillus subtilis L2 on plant growth and physiological properties of ginger. The experiment was carried out in randomised block design with three replications in pot experiments. The plants were grown in greenhouse conditions for three months. The results showed that at 8 and 12 weeks after planting (WAP) bacterial inoculation increased plant height, leaf length, number of leaves per plant and leaf width. Inoculation with B. subtilis L2 significantly increased plant height by 16, 20 and 18% compared to control at 4, 8 and 12 WAP. At 8 and 12 WAP, leaf length significantly raised by B. subtilis L2 as compared to uninoculated control. B. subtilis L2 significantly increased the number of leaves per plant and leaf width by 30 and 21% respectively when comparing with non-inoculated plants at 8 WAP. The percentage increase in chlorophyll content resulted from the inoculation with B. subtilis L2 over the control was 10.5%, 15.5% and 18.4% at 4, 8 and 12 WAP respectively. It is concluded that there is a significant positive effect of inoculation with B. subtilis L2 on the growth of ginger. B. subtilis L2 strain can be used as a potential agent or bio-fertiliser for stimulation of ginger growth.

scholarly journals Perbanyakan dan Aplikasi PGPR (Plant Growth Promoting Rhizobactheria) di Laboratorium Pengamatan Hama dan Penyakit Banyumas

2021 ◽  
Vol 2 ◽  
pp. 234-239
Author(s):  
Nada Kholifah ◽  
Ardiana Kartika B ◽  
Teguh Pribadi
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Root Length ◽  
Dry Weight ◽  
Test Plant ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Test Plants

PGPR (Plant Growth Promoting Rhizobacteria) is a substance that helps plant growth with the help of rhizosphere microorganisms. PGPR propagation can be done with liquid media. This PGPR propagation needs to be done because this substance has many benefits for agricultural cultivation. The application of PGPR to the test plant, namely the pakcoy plant, proved that there was an effect of giving PGPR to the plant. Observations on the test plants were carried out by observing several observation variables such as plant height, root length, number of leaves, wet weight and dry weight. The results of these observations showed that the effect on the test plants was seen in the variables of root length, wet weight, and dry weight. Meanwhile, the variable plant height and number of leaves did not show a visible difference. 

scholarly journals Uji Penggunaan PGPR (Plant Growth-Promoting Rhizobacteria) terhadap Pertumbuhan Vegetatif Tanaman Cabai Merah (capsicum Annuum L.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 150
Author(s):  
Lidyanti Ollo ◽  
Parluhutan Siahaan ◽  
Beivy Kolondam
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Capsicum Annuum ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Root Volume ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Growth Promoting

Penelitian ini bertujuan yang menguji Perbandingan pertumbuhan vegetatif tanaman cabai merah (capsicum Annuum L.) akibat Pemberian Komposisi PGPR (Plant Growth-Promoting Rhizobacteria) yang perbeda. Pertumbuhan tanaman diukur berdasarkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman. Hasil penelitian menujukkan bahwa PGPR dapat meningkatkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman cabe.Tinggi tanaman terbaik setelah diperlakuan selama 28 hari adalah dengan pemberian PGPR disertai dengan pemberian pupuk kandang dengan tinggi (24.44 cm), yang diikuti oleh pemberian PGPR + kompos dengan tinggi (22.04 cm), dan pemberian PGPR + NPK dengan tnggi tanaman 19.68 cm . Volume akar akan lebih tinggi bila diberikan PGPR yang ditambah dengan pupuk kandang , atau kompos maupun NPK, tetapi tdak berbeda hasilnya bila hanya diberikan PGPR saja. Jumlah daun dapat ditingkatkan bila diberikan PGPR yang ditambah dengan pupuk kandang, atau kompos maupun NPK. Pemberian PGPR + NPK tidak memberikan jumlah daun yang berbeda dengan pemberian PGPR saja.Berat basah akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basah.Berat kering akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basahThis study aimed to examine the comparison of vegetative growth of red chili (capsicum Annuum L.) due to the administration of different PGPR (Plant Growth-Promoting Rhizobacteria) Composition. Plant growth was measured based on plant height, number of leaves, root volume, wet weight and dry weight of the plant. The results showed that PGPR can increase plant height, number of leaves, root volume, wet weight and dry weight of chilli plants. The best plant height after being treated for 28 days is by giving PGPR accompanied by administering manure with height (24.44 cm), followed by by giving PGPR + compost with a height (22.04 cm), and giving PGPR + NPK with a plant height of 19.68 cm. Root volume will be higher if given PGPR added with manure, or compost or NPK, but the results are not different if only given PGPR. The number of leaves can be increased if given PGPR added with manure, or compost or NPK. Provision of PGPR + NPK does not provide a number of leaves that are different from the administration of PGPR alone. Wet weight increased when given PGPR alone or added with manure, or compost or NPK. PGPR added with manure or compost gives the same effect on wet weight. Dry weight will increase if given PGPR alone or added with manure, or compost or NPK. PGPR coupled with manure or compost has the same effect on wet weight

scholarly journals THE RHIZOSPHERE PGPR GRAMINEAE APPLICATION ON THE GROWTH RED GINGER (Zingiber officinale Var. Rubrum)

2017 ◽  
Vol 4 (2) ◽  
pp. 133
Author(s):  
Hesti Kurniahu
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Zingiber Officinale ◽  
Optimal Dosage ◽  
Bacterial Group ◽  
Ginger Rhizome ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Red Ginger

<p><em>PGPR (Plant Growth Promoting Rhizobacteria) is a group of bacteria that colonize in the area of the rhizosphere. These groups of bacteria favor plant growth because they are able to mobilize and provide nutrients and phytohormones. The ability of the bacterial group can be utilized as a growth regulator in the red ginger nursery (Z. officinale Var Rubrum). This study aims to determine the optimal dosage of PGPR for the growth of red ginger seed (Z. officinale Var Rubrum). In this study, red ginger rhizome (Z. officinale Var Rubrum) was soaked with various concentrations of PGPR ie 0%, 25%, 50%, 75% and 100%. The results of this study indicate that soaking rhizomes using PGPR solution significantly influence the growth of red ginger seeds in the form of high seedlings, the number of shoots grow, the number of leaves and the mass of seedlings. A 25% dose has a better ability to boost the growth of seeds when compared to other doses.</em><strong></strong></p><p><em><br /></em><strong></strong></p><p><strong>Keywords: </strong><strong><em>Zingiber officinale Var. Rubrum</em></strong><strong><em>, PGR, Phytohormone</em></strong><strong>. </strong></p>

UPAYA PENGGENDALIAN PENYAKIT LAYU STEWART (Pantoea stewartii subsp. stewartii) PADA TANAMAN JAGUNG MENGGUNAKAN RIZOBAKTERIA

Jurnal BiBieT ◽  
2017 ◽  
Vol 2 (1) ◽  
pp. 8
Author(s):  
Yulfi Desi ◽  
Prima Novia
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Wilt Disease ◽  
Pantoea Stewartii ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Male Flowers ◽  
Stewart's Wilt ◽  
Corn Plants

<p>Penyakit layu Stewart pada tanaman jagung disebabkan oleh bakteri <em>Pantoea stewartii</em> subsp. <em>stewartii</em>, merupakan penyakit penting karena dapat mengakibatkan kehilangan hasil. Di Indonesia, penyakit layu Stewart masih tergolong baru dan belum ditemukan cara pengendaliannya. Penelitian bertujuan untuk mengupayakan pengendalian penyakit layu Stewart menggunakan Rizobakteria yang berperan sebagai PGPR (<em>Plant Growth Promoting Rizobacteria</em>) agar dapat meningkatkan pertumbuhan tanaman jagung sekaligus mengurangi laju perkembangan penyakit layu Stewart. Penelitian berupa percobaan lapangan di dalam polybag menggunakan RAK (Rancangan Acak Kelompok) dengan 11 perlakuan dan 3 ulangan. Sebagai perlakuan adalah beberapa isolat Rizobakteria yang diperlakukan pada benih jagung. Inokulasi patogen <em>P. stewartii</em> subsp. <em>stewartii</em> dilakukan pada bibit jagung umur 7 hst (hari setelah tanam). Parameter pengamatan antara lain: 1) perkembangan penyakit meliputi: periode inkubasi (hari) dan severitas penyakit (%). 2) pertumbuhan tanaman meliputi: tinggi tanaman (cm), diameter batang (mm), jumlah daun (helai), lebar daun ke-7 (cm), panjang daun ke-7 (cm), muncul bunga jantan (hari), muncul bunga betina (hari), berat buah per tanaman (gr), dan diameter buah tanpa kelobot (mm). Hasil penelitian mendapatkan penggunaan Rizobakteria isolat BRb 251 dapat meningkatkan tinggi tanaman, jumlah daun, dan hasil per tanaman jagung. Dengan demikian, berpotensi sebagai PGPR dalam meningkatkan pertumbuhan tanaman jagung, meskipun belum mampu menunjukkan perannya dalam menurunkan laju perkembangan penyakit layu Stewart.</p><p> </p><p><em>Stewart's wilt disease in corn plants caused by the bacteria Pantoea stewartii subsp. stewartii, that is important because it can cause yield losses. In Indonesia, Stewart’s wilt disease is still relatively new and has not found the way of control. This study aims to seek ways of controlling Stewart's wilt disease by using Rizobakteria which is likely to play a role as PGPR (Plant Growth Promotes Rizobakteria) in order to increase the growth of corn plants while reducing the rate of progression of Stewart’s wilt disease. This research trials in polybag using RAK (Group Random Design) with 11 treatments and 3 replications. As a treatment is several isolates rizobactria treat to seedcorn. Bacterial inoculation was performed on the seeds of maize 7 dap (days after planting). The observation parameters were: 1) disease progression (incubation period (day) and severity of disease (%). 2) plant growth (plant height (cm), stem diameter (mm), number of leaves (strands), width of the leaves of the 7<sup>th</sup> (cm), length of the leaves of the 7<sup>th</sup>  (cm), emerging male flowers (day), female flowers appear (day), weight of ear per plant (gr), and ear diameter without husk (mm). Result from this research, found that using rizobacteria isolate BRB 251 can increase: plant height, number of leaves, and yield per plant, so that potential as PGPR in increasing the growth of corn plants, although not yet able to demonstrate its role in reducing the rate of development of Stewart wilt disease.</em></p>

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

Film coating of seeds with Bacillus cereus RS87 spores for early plant growth enhancement

2008 ◽  
Vol 54 (10) ◽  
pp. 861-867 ◽  
Author(s):  
Kanchalee Jetiyanon ◽  
Sakchai Wittaya-Areekul ◽  
Pinyupa Plianbangchang
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Plant Height ◽  
Root Length ◽  
Film Coating ◽  
Field Trials ◽  
Growth Enhancement ◽  
Vegetative Cells ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacterium Bacillus cereus RS87 was previously reported to promote plant growth in various crops in both greenhouse and field trials. To apply as a plant growth promoting agent with practical use, it is essential to ease the burden of routine preparation of a fresh suspension of strain RS87 in laboratory. The objectives of this study were to investigate the feasibility of film-coating seeds with B. cereus RS87 spores for early plant growth enhancement and to reveal the indoleacetic acid (IAA) production released from strain RS87. The experiment consisted of the following 5 treatments: nontreated seeds, water-soaked seeds, film-coated seeds, seeds soaked with vegetative cells of strain RS87, and film-coated seeds with strain RS87 spores. Three experiments were conducted separately to assess seed emergence, root length, and plant height. Results showed that both vegetative cells and spores of strain RS87 significantly promoted (P ≤ 0.05) seed emergence, root length and plant height over the control treatments. The strain RS87 also produced IAA. In conclusion, the film coating of seeds with spores of B. cereus RS87 demonstrated early plant growth enhancement as well as seeds using their vegetative cells. IAA released from strain RS87 would be one of the mechanisms for plant growth enhancement.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals Plant Growth Promotion and Biocontrol Properties of Aneurinibacillus migulanus Isolated from Maize Roots (Zea mays)

2021 ◽  
pp. 46-59
Author(s):  
Lynda Kelvin Asogwa ◽  
Frank C. Ogbo
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Plant Height ◽  
Pot Experiment ◽  
Root Weight ◽  
Dual Culture ◽  
Maize Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting

Aims: To isolate Plant Growth Promoting Bacillus strain from maize roots, to evaluate its biocontrol potentials and to characterize the isolate using16S rRNA sequencing. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, Awka, between February 2019 and March 2020. Methodology: The isolation of Plant Growth Promoting Rhizobacteria (PGPR) from maize roots was done using Pikovskaya (PVK) agar. Quantitative determination of phosphate was carried out using PVK broth. Evaluations of other plant growth promoting properties were carried out such as IAA, etc. Fusarium and Enterobacter plant pathogens were isolated from diseased maize plants. The in vitro antagonism effects of the PGPR isolates against the pathogens were analyzed using the dual culture plate technique. The pot experiment was carried out in a completely randomized design. Plant characteristics such as plant height, shoot  and root weight, chlorophyll content, as well as disease assessment were recorded accordingly. The organisms were identified using phenotypic and molecular methods. Results: Seven PGPR bacteria were isolated from maize (Zea mays) roots using PVK agar. Aneurinibacillus migulanus gave the highest solubilization index of 4.21 while isolate IS48 gave the lowest solubilization index of 1.47. A. migulanus produced IAA, ammonia and cellulase enzyme but no hydrogen cyanide. The organism showed antagonism activity against the two tested phytopathogens. In the pot experiment, A. migulanus treated plants showed a statistically insignificant difference in maize plant height at P=0.05 but gave significant increases in shoot and root wet weights. The organism offered 83.33% and 71.43% protection against Enterobacter and Fusarium pathogens respectively in the pot experiment. Conclusion: A. migulanus solubilized phosphate in addition to other plant growth promoting  properties. It showed biocontrol potentials both in vitro and in vivo and thus can be used as substitute for synthetic agrochemicals.

scholarly journals Quorum quenching activity of the PGPR Bacillus subtilis UD1022 alters nodulation efficiency of Sinorhizobium meliloti on Medicago truncatula

2020 ◽  
Author(s):  
Amanda Rosier ◽  
Pascale B. Beauregard ◽  
Harsh P. Bais
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Quorum Quenching ◽  
Agricultural System ◽  
Synergistic Activity ◽  
Sustainable Solutions ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPlant growth promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a ‘consortia’ approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530 and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model ‘consortium’. These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.

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