scholarly journals Perbanyakan dan Aplikasi PGPR (Plant Growth Promoting Rhizobactheria) di Laboratorium Pengamatan Hama dan Penyakit Banyumas

2021 ◽  
Vol 2 ◽  
pp. 234-239
Author(s):  
Nada Kholifah ◽  
Ardiana Kartika B ◽  
Teguh Pribadi
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Root Length ◽  
Dry Weight ◽  
Test Plant ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Test Plants

PGPR (Plant Growth Promoting Rhizobacteria) is a substance that helps plant growth with the help of rhizosphere microorganisms. PGPR propagation can be done with liquid media. This PGPR propagation needs to be done because this substance has many benefits for agricultural cultivation. The application of PGPR to the test plant, namely the pakcoy plant, proved that there was an effect of giving PGPR to the plant. Observations on the test plants were carried out by observing several observation variables such as plant height, root length, number of leaves, wet weight and dry weight. The results of these observations showed that the effect on the test plants was seen in the variables of root length, wet weight, and dry weight. Meanwhile, the variable plant height and number of leaves did not show a visible difference. 

scholarly journals Uji Penggunaan PGPR (Plant Growth-Promoting Rhizobacteria) terhadap Pertumbuhan Vegetatif Tanaman Cabai Merah (capsicum Annuum L.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 150
Author(s):  
Lidyanti Ollo ◽  
Parluhutan Siahaan ◽  
Beivy Kolondam
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Capsicum Annuum ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Root Volume ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Growth Promoting

Penelitian ini bertujuan yang menguji Perbandingan pertumbuhan vegetatif tanaman cabai merah (capsicum Annuum L.) akibat Pemberian Komposisi PGPR (Plant Growth-Promoting Rhizobacteria) yang perbeda. Pertumbuhan tanaman diukur berdasarkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman. Hasil penelitian menujukkan bahwa PGPR dapat meningkatkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman cabe.Tinggi tanaman terbaik setelah diperlakuan selama 28 hari adalah dengan pemberian PGPR disertai dengan pemberian pupuk kandang dengan tinggi (24.44 cm), yang diikuti oleh pemberian PGPR + kompos dengan tinggi (22.04 cm), dan pemberian PGPR + NPK dengan tnggi tanaman 19.68 cm . Volume akar akan lebih tinggi bila diberikan PGPR yang ditambah dengan pupuk kandang , atau kompos maupun NPK, tetapi tdak berbeda hasilnya bila hanya diberikan PGPR saja. Jumlah daun dapat ditingkatkan bila diberikan PGPR yang ditambah dengan pupuk kandang, atau kompos maupun NPK. Pemberian PGPR + NPK tidak memberikan jumlah daun yang berbeda dengan pemberian PGPR saja.Berat basah akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basah.Berat kering akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basahThis study aimed to examine the comparison of vegetative growth of red chili (capsicum Annuum L.) due to the administration of different PGPR (Plant Growth-Promoting Rhizobacteria) Composition. Plant growth was measured based on plant height, number of leaves, root volume, wet weight and dry weight of the plant. The results showed that PGPR can increase plant height, number of leaves, root volume, wet weight and dry weight of chilli plants. The best plant height after being treated for 28 days is by giving PGPR accompanied by administering manure with height (24.44 cm), followed by by giving PGPR + compost with a height (22.04 cm), and giving PGPR + NPK with a plant height of 19.68 cm. Root volume will be higher if given PGPR added with manure, or compost or NPK, but the results are not different if only given PGPR. The number of leaves can be increased if given PGPR added with manure, or compost or NPK. Provision of PGPR + NPK does not provide a number of leaves that are different from the administration of PGPR alone. Wet weight increased when given PGPR alone or added with manure, or compost or NPK. PGPR added with manure or compost gives the same effect on wet weight. Dry weight will increase if given PGPR alone or added with manure, or compost or NPK. PGPR coupled with manure or compost has the same effect on wet weight

Film coating of seeds with Bacillus cereus RS87 spores for early plant growth enhancement

2008 ◽  
Vol 54 (10) ◽  
pp. 861-867 ◽  
Author(s):  
Kanchalee Jetiyanon ◽  
Sakchai Wittaya-Areekul ◽  
Pinyupa Plianbangchang
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Plant Height ◽  
Root Length ◽  
Film Coating ◽  
Field Trials ◽  
Growth Enhancement ◽  
Vegetative Cells ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacterium Bacillus cereus RS87 was previously reported to promote plant growth in various crops in both greenhouse and field trials. To apply as a plant growth promoting agent with practical use, it is essential to ease the burden of routine preparation of a fresh suspension of strain RS87 in laboratory. The objectives of this study were to investigate the feasibility of film-coating seeds with B. cereus RS87 spores for early plant growth enhancement and to reveal the indoleacetic acid (IAA) production released from strain RS87. The experiment consisted of the following 5 treatments: nontreated seeds, water-soaked seeds, film-coated seeds, seeds soaked with vegetative cells of strain RS87, and film-coated seeds with strain RS87 spores. Three experiments were conducted separately to assess seed emergence, root length, and plant height. Results showed that both vegetative cells and spores of strain RS87 significantly promoted (P ≤ 0.05) seed emergence, root length and plant height over the control treatments. The strain RS87 also produced IAA. In conclusion, the film coating of seeds with spores of B. cereus RS87 demonstrated early plant growth enhancement as well as seeds using their vegetative cells. IAA released from strain RS87 would be one of the mechanisms for plant growth enhancement.

UPAYA PENGGENDALIAN PENYAKIT LAYU STEWART (Pantoea stewartii subsp. stewartii) PADA TANAMAN JAGUNG MENGGUNAKAN RIZOBAKTERIA

Jurnal BiBieT ◽  
2017 ◽  
Vol 2 (1) ◽  
pp. 8
Author(s):  
Yulfi Desi ◽  
Prima Novia
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Wilt Disease ◽  
Pantoea Stewartii ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting ◽  
Male Flowers ◽  
Stewart's Wilt ◽  
Corn Plants

<p>Penyakit layu Stewart pada tanaman jagung disebabkan oleh bakteri <em>Pantoea stewartii</em> subsp. <em>stewartii</em>, merupakan penyakit penting karena dapat mengakibatkan kehilangan hasil. Di Indonesia, penyakit layu Stewart masih tergolong baru dan belum ditemukan cara pengendaliannya. Penelitian bertujuan untuk mengupayakan pengendalian penyakit layu Stewart menggunakan Rizobakteria yang berperan sebagai PGPR (<em>Plant Growth Promoting Rizobacteria</em>) agar dapat meningkatkan pertumbuhan tanaman jagung sekaligus mengurangi laju perkembangan penyakit layu Stewart. Penelitian berupa percobaan lapangan di dalam polybag menggunakan RAK (Rancangan Acak Kelompok) dengan 11 perlakuan dan 3 ulangan. Sebagai perlakuan adalah beberapa isolat Rizobakteria yang diperlakukan pada benih jagung. Inokulasi patogen <em>P. stewartii</em> subsp. <em>stewartii</em> dilakukan pada bibit jagung umur 7 hst (hari setelah tanam). Parameter pengamatan antara lain: 1) perkembangan penyakit meliputi: periode inkubasi (hari) dan severitas penyakit (%). 2) pertumbuhan tanaman meliputi: tinggi tanaman (cm), diameter batang (mm), jumlah daun (helai), lebar daun ke-7 (cm), panjang daun ke-7 (cm), muncul bunga jantan (hari), muncul bunga betina (hari), berat buah per tanaman (gr), dan diameter buah tanpa kelobot (mm). Hasil penelitian mendapatkan penggunaan Rizobakteria isolat BRb 251 dapat meningkatkan tinggi tanaman, jumlah daun, dan hasil per tanaman jagung. Dengan demikian, berpotensi sebagai PGPR dalam meningkatkan pertumbuhan tanaman jagung, meskipun belum mampu menunjukkan perannya dalam menurunkan laju perkembangan penyakit layu Stewart.</p><p> </p><p><em>Stewart's wilt disease in corn plants caused by the bacteria Pantoea stewartii subsp. stewartii, that is important because it can cause yield losses. In Indonesia, Stewart’s wilt disease is still relatively new and has not found the way of control. This study aims to seek ways of controlling Stewart's wilt disease by using Rizobakteria which is likely to play a role as PGPR (Plant Growth Promotes Rizobakteria) in order to increase the growth of corn plants while reducing the rate of progression of Stewart’s wilt disease. This research trials in polybag using RAK (Group Random Design) with 11 treatments and 3 replications. As a treatment is several isolates rizobactria treat to seedcorn. Bacterial inoculation was performed on the seeds of maize 7 dap (days after planting). The observation parameters were: 1) disease progression (incubation period (day) and severity of disease (%). 2) plant growth (plant height (cm), stem diameter (mm), number of leaves (strands), width of the leaves of the 7<sup>th</sup> (cm), length of the leaves of the 7<sup>th</sup>  (cm), emerging male flowers (day), female flowers appear (day), weight of ear per plant (gr), and ear diameter without husk (mm). Result from this research, found that using rizobacteria isolate BRB 251 can increase: plant height, number of leaves, and yield per plant, so that potential as PGPR in increasing the growth of corn plants, although not yet able to demonstrate its role in reducing the rate of development of Stewart wilt disease.</em></p>

scholarly journals Utilization Of Organic Fertilizer Compost Made From Purple Sweet Potato Waste (Ipomoea Batatas L.) To Increase The Production Of Pakchoy (Brassica Chinensis L.)

2021 ◽  
Vol 5 (3) ◽  
Author(s):  
Sheila Dwi Shilviana ◽  
Ni Luh Suriani ◽  
I Ketut Sundra
Keyword(s):  
Plant Height ◽  
Sweet Potato ◽  
Root Length ◽  
Organic Waste ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Potato Waste ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Purple Sweet Potato

Compost is an organic fertilizer made from recycled organic materials. Fertilizers can be made from organic waste or unused organic waste. Purple sweet potato waste can be reused into compost. The purpose of this study was to examine the effectiveness of purple sweet potato waste compost inoculated with Trichoderma harzianum in increasing the production of pakchoi and to determine the treatment of purple sweet potato waste compost to increase the production of pakchoi. The research method used is the experimental method. The experimental design used was RAK (Randomized Block Design) with 1 factor. The experiment consisted of 4 treatments of purple sweet potato compost 25 (B) g, 50 g (C), 75 g (D), 150 g (E), and 1 control (A), and was repeated 5 times. The data analysis technique used analysis of variance (ANOVA). Parameters observed were the number of leaves, plant height, leaf width, plant wet weight, plant dry weight, and root length. The results showed that the application of purple sweet potato compost affected increasing the growth and production of pakchoi. The right treatment in increasing the growth of pakchoi is treatment 150 g or treatment E. Treatment 150 g (E) can increase the number of leaves (13.13), plant height (16.32 cm), leaf width (4.88 cm), wet weight (16.60 g), dry weight (6.35 g), and root length (15.80 cm).

scholarly journals Plant growth promoting bacteria Bacillus subtilis promote growth and physiological parameters of Zingiber officinale Roscoe

2021 ◽  
Vol 8 (1) ◽  
pp. 66-71
Author(s):  
D Jabborova ◽  
Y Enakiev ◽  
Khurshid Sulaymanov ◽  
Dilbar Kadirova ◽  
Ahmad Ali ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Height ◽  
Zingiber Officinale ◽  
Leaf Length ◽  
Leaf Width ◽  
Zingiber Officinale Roscoe ◽  
Plant Growth Promoting ◽  
Number Of Leaves ◽  
Growth Promoting

Ginger (Zingiber officinale Roscoe) is an important medicinal crop grown for its aromatic rhizome which is used as a spice, food, flavouring agent and medicine. It has been characterised for its hypoglycemic, hypotensive, antioxidant and antibiotic properties. This study was conducted to determine the impact of plant growth-promoting potential of bacterial strain Bacillus subtilis L2 on plant growth and physiological properties of ginger. The experiment was carried out in randomised block design with three replications in pot experiments. The plants were grown in greenhouse conditions for three months. The results showed that at 8 and 12 weeks after planting (WAP) bacterial inoculation increased plant height, leaf length, number of leaves per plant and leaf width. Inoculation with B. subtilis L2 significantly increased plant height by 16, 20 and 18% compared to control at 4, 8 and 12 WAP. At 8 and 12 WAP, leaf length significantly raised by B. subtilis L2 as compared to uninoculated control. B. subtilis L2 significantly increased the number of leaves per plant and leaf width by 30 and 21% respectively when comparing with non-inoculated plants at 8 WAP. The percentage increase in chlorophyll content resulted from the inoculation with B. subtilis L2 over the control was 10.5%, 15.5% and 18.4% at 4, 8 and 12 WAP respectively. It is concluded that there is a significant positive effect of inoculation with B. subtilis L2 on the growth of ginger. B. subtilis L2 strain can be used as a potential agent or bio-fertiliser for stimulation of ginger growth.

scholarly journals Effect of Yeast Based Biofertilizer combined with bacteria on Mustard Plant Growth

2017 ◽  
Vol 1 (2) ◽  
Author(s):  
Nur Hidayatul Alami
Keyword(s):  
Plant Growth ◽  
Root Length ◽  
Dry Weight ◽  
Biological Processes ◽  
Microbial Inoculants ◽  
A Value ◽  
Mustard Plant ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Phosphate Solubilizer

Biofertilizers are commonly defined as microbial inoculants which are capable of mobilizing important nutrient in the soil from non-available to available form for the plants through their biological processes. Hence to increase the productivity of the soil, the use of biofertilizer is necessary. Candida yeast as a phosphate solubilizer and decomposer. Azotobacter bacteria as a nitrogen-fixing bacteria and Bacillus as a phosphate solubilizer. The combination of these microorganisms as biofertilizer needs to be tested its effect against mustard (Brassica juncea L.) plant growth.The results showed that the combination of Bacillus Candida obtained the highest yield among other biofertilizer treatments with a value of 13.6 cm at plant height, 4.96 grams in wet weight and 2.29 grams in dry weight. The combination of Azotobacter Candida showed the highest result on the parameter of width and number of leaves with the values of 20.3 cm and 5.3. Combination of Azotobacter Bacillus Candida showed the highest yield on the root length parameter with a value of 5.0 cm. The addition of biofertilizer is expected to reduce pollution on the soil due to residues produced by chemical fertilizers.Keyword : Azotobacter, Bacillus, Biofertilizer, Candida, Mustard Plant

Characterization and evaluation of plant growth promoting Pseudomonas isolated from rhizosphere of Acacia nilotica and Albizia lebbeck

2017 ◽  
Vol 51 (04) ◽  
Author(s):  
S. K. Althaf Hussain ◽  
Hussain Banoth ◽  
Dayakar Govindu ◽  
P. Srinivas
Keyword(s):  
Plant Growth ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Acacia Nilotica ◽  
Test Plant ◽  
Toxic Substances ◽  
Albizia Lebbeck ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Test Plants

A total of 65 bacterial isolates from 10 different locations of the test plants i.e. Acacia nilotica and Albizia lebbeck in Godavari belt region were screened. Of which, seven isolates showed positive response to plant growth promoting characteristics like Indole Acetic Acid [IAA), production of volatile toxic substances like Hydrogen sulfide [H2S) and Hydrogen cyanide [HCN) that are antagonistic in nature. These screened isolates were tested for germination efficiency on test plant seeds. It was found that Isolate number-13[Ps-13] was very efficient on seed germination. To test the efficient combination of bioinoculants, the best plant growth promoting Pseudomonas species was applied to test plants along with other standard microbial bioinoculants and the result showed that Pseudomonas along with Glomus species [Arbuscular Mycorrhizal Fungus] gave best results for Albizia lebbeck. Rhizobium and Pseudomonas combination was found to be best for Acacia nilotica.

scholarly journals PROVISION OF MICRORHIZA FUNGI AGAINST SALINE STRESS IN CHILI PLANTS

2021 ◽  
Vol 9 (10) ◽  
pp. 242-246
Author(s):  
Mizan Maulana ◽  
◽  
Zaitun Ritaqwin ◽  
Firda Annisa ◽  
◽  
...  
Keyword(s):  
Plant Height ◽  
Root Length ◽  
Dry Weight ◽  
Fruit Weight ◽  
Stem Diameter ◽  
Plant Production ◽  
Root Weight ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Screen House

This experiment was a factorial randomized block design (RAK). The screen house used was the screen house of the experimental garden of the Faculty of Agricultural and Animal Sciences, Indonesian National Islamic University. The land was taken from the coast of Kuala Kab. Bireuen, Aceh. Soil was taken from the top soil layer with a depth of 0-25 cm. Mycorrhizae were placed in planting holes around the root area at a depth of 2-3 cm according to the treatment per plant before the seeds were planted in polybags. Then the planting hole that has been given mycorrhizae is covered again with soil. Mycorrhizal application was carried out once at the time of seed planting. Harvesting can be done by picking the fruit and its stalk. The analysis was carried out to determine the level of ability of mycorrhizal species to help plants in the absorption of P nutrients to maximize chili plant production, the effect of chili varieties had a significant effect on root infection at 45 DAP, a very significant effect on stem diameter at 15 DAP, wet root weight, number of fruit planted, dry weight. fruit, had a significant effect on plant height at 15 DAP, stem diameter at 30 DAP and fruit weight at planting, but had no significant effect on plant height at 30 and 45 DAP, stem diameter at 45 DAP, number of leaves at 15, 30 and 45 DAP, weight dry root, root wet weight, root dry weight, root length, number of productive branches 45 DAP. The results showed that the variety had a very significant effect on the growth of chili plants. The best variety was found in the Lado variety in the vegetative phase which could be seen from the variables of plant height 15 and 30 DAP, stem diameter at 15 and 30 DAP, number of leaves at 15, 30 and 45 DAP. While the generative phase of the best variety was found in the PM 999 variety which could be seen in the variables Number of fruit, fruit weight, dry weight, root wet weight and root length.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

Isolation, identification and characterization of native plant growth promoting bacteria and their plant growth promotion in Zea mays

2021 ◽  
Vol 16 (8) ◽  
pp. 75-80
Author(s):  
Pitchaiah Pelapudi ◽  
Sasikala Ch ◽  
Swarnabala Ganti
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Length ◽  
Growth Promotion ◽  
Shoot Length ◽  
Root Weight ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Shoot Weight

In the present rapid growing world, need for a sustainable agricultural practice which helps in meeting the adequate food demand is much needed. In this context, plant growth promoting bacteria were brought into the spot light by the researchers. Though the plant growth promoting bacteria have several beneficial applications, due to some of the disadvantages in the field conditions, they lagged behind. In the current research work, native PGPR were isolated from the rhizosphere soil samples of maize with an aim to isolate the nitrogen fixing, phosphate solubilising and potash solubilising bacteria. Out of the several isolates, potent PGPR isolates viz., Paenibacillus durus PCPB067, Bacillus megaterium PCBMG041 and Paenibacillus glucanolyticus PCPG051 were isolated and identified by using the 16 S rRNA gene sequencing studies. Genomic DNA sequences obtained were deposited in the NCBI Genbank and accession numbers were assigned as MW793452, MW793456 and MW843633. In order to check the efficacy of the PGPR isolates, pot trials were conducted by taking maize as the host plant. Several parameters viz. shoot length, shoot weight, root length, root weight and weight of the seeds were tested in which PGP treatment showed good results (shoot length - 187±3.5 cm, shoot weight - 31±4 g, root length - 32±3.6 cm, root weight - 17±2 g, yield- 103.3±6.1 g) when compared to the chemical fertilizer treatment (shoot length - 177±3.5 cm, shoot weight - 25±3.6 g, root length - 24±3.5 cm, root weight - 14.6±1.52 g, yield- 85.6±7.6 g). Based on the results, it can be stated that these native PGPR isolates can be effectively used in the plant growth promotion of maize.

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