scholarly journals Changes in matrix metalloproteinases and their inhibitors in the tears and blood serum of patients with herpetic keratoconjunctivitis under the influence of antiherpetic antibodies and nimesulide

Pathologia ◽  
2021 ◽  
Vol 18 (1) ◽  
pp. 50-57
Author(s):  
P. H. Pantielieiev ◽  
I. S. Haidash
Keyword(s):  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Acute Phase ◽  
Solid Phase ◽  
Tear Fluid ◽  
Control Group ◽  
Tissue Inhibitors ◽  
Lacrimal Fluid ◽  
Simplex Virus ◽  
Convalescence Phase

Aim. The aim of the study was to study changes in matrix metalloproteinases and their tissue inhibitors in the tears and blood serum of patients with herpetic keratoconjunctivitis under the influence of antiherpetic antibodies and nimesulide. Materials and methods.65 adult patients with herpetic keratoconjunctivitis (HKC) caused by the herpes simplex virus type 1 (HSV-1) were examined. The etiological diagnosis of HKC was confirmed by polymerase chain reaction. The control group consisted of 32 patients who received basic treatment with acyclovir and interferon in the affected eye. The main group consisted of 33 patients who received antiherpetic immunoglobulin and nimesulide in addition to basic therapy. Immunoglobulin was administered intramuscularly and instilled into the affected eye, and nimesulide was administered orally. The control group consisted of 38 practically healthy adults. Matrix metalloproteinases MMP-1, MMP-8, and MMP-9 and their tissue inhibitors TIMP-1 and TIMP-2 were determined by solid-phase enzyme immunoassay in the tear fluid and blood serum of patients. The results of the study were processed according to the Student's criterion. Results. With HKC in the affected eye, in the acute phase of the disease, there are: an increase in the concentrations of MMP-1, MMP-8, MMP-9, both in the tear fluid and in the blood serum of patients; a decrease in the concentrations of TIMP-1 and TIMP-2 in the tear fluid, with unchanged levels of these compounds in the blood serum; violation of the balance in the corresponding MMP/TIMP systems to the advantage of MMP. In the convalescence phase, these shifts in the concentrations of MMPs, TIMPs and the balance between them significantly decrease, but their complete normalization does not occur, which is most significant for tear fluid than for the blood serum of convalescents. The combination of antiherpetic immunoglobulin and nimesulide helps to inhibit the exces production of MMP-1, MMP-8, MMP-9 in the lacrimal fluid of the causal eye and in the blood serum of patients, optimizes the level of TIMP-1, TIMP-2 and balance in the corresponding MMPs/TIMPs systems. Conclusion. In the acute phase of SCC, MMPs activity prevails, in the convalescence phase, TIMPs activity prevails. The combination of antiherpetic immunoglobulin and nimesulide has a positive effect on the state of MMPs and TIMPs in the lacrimal fluid of the causal eye and in the blood serum of patients.

scholarly journals Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Chronic Rhinosinusitis With Nasal Polyps: Etiopathogenesis and Recurrence

2020 ◽  
pp. 014556131989663
Author(s):  
Germano Guerra ◽  
Domenico Testa ◽  
Francesco Antonio Salzano ◽  
Domenico Tafuri ◽  
Eleonora Hay ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Matrix Metalloproteinases ◽  
Chronic Rhinosinusitis ◽  
Nasal Polyps ◽  
Polymerase Chain Reaction Analysis ◽  
Sinus Surgery ◽  
Control Group ◽  
Nonallergic Rhinitis ◽  
Tissue Inhibitors ◽  
Immunologic Diseases

Chronic rhinosinusitis with nasal polyps is a multifactorial disease of the nasal and paranasal sinus mucosa and it includes, as comorbidities, anatomic and morphologic alterations, allergic rhinitis, and immunologic diseases. We investigated matrix metalloproteinases (MMP-2, MMP-7, and MMP-9) and their tissue inhibitors (TIMP-1 and TIMP-2) concentration in different etiopathogenetical groups of patients with nasal polyposis (NP) in relation to recurrence after sinonasal surgery. The study group consisted of 45 patients with NP (those with allergic rhinitis, nonallergic rhinitis and asthma or nonallergic rhinitis, and obstruction of osteomeatal complex [OMC]) who underwent endonasal sinus surgery. We also collected 10 patients who underwent septoplasty as control. Immunohistochemistry of nasal mucosa fragments, Western blotting, and polymerase chain reaction analysis showed increased MMPs levels (MMP-9 more than MMP-2 and MMP-7) and decreased tissue inhibitors of MMPs levels (TIMP-1 less than TIMP-2), in patients with chronic rhinosinusitis with nasal polyps compared with control group, in particular in patients with nonallergic rhinitis and asthma compared to those with allergic rhinitis and nonallergic rhinitis and obstruction of OMC. We observed a higher risk of recurrence in patients with nonallergic rhinitis and asthma than in those with allergic rhinitis and nonallergic rhinitis and obstruction of OMC after 36 months from surgery. In this research, we evaluated pathogenesis of NP related to MMPs and their inhibitors concentrations in polypoid tissue.

scholarly journals The Unwounded Skin Remodeling in Animal Models of Diabetes Types 1 and 2

2013 ◽  
pp. 519-526 ◽  
Author(s):  
M. KNAŚ ◽  
M. NICZYPORUK ◽  
A. ZALEWSKA ◽  
H. CAR
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Diabetes Type ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitors Of Metalloproteinases ◽  
Control Group ◽  
Diabetes Type 1 ◽  
Tissue Inhibitors ◽  
The Matrix

Diabetes mellitus types 1 and 2 are chronic diseases that cause serious health complications, including dermatologic problems. The diabetic skin is characterized by disturbances in collagen metabolism. A tissue remodeling depends on the degradation of extracellular matrix through the matrix metalloproteinases, which are regulated by e.g. the tissue inhibitors of metalloproteinases. The balance between matrix metalloproteinases (MMPs) and tissue inhibitors of matrix metalloproteinases (TIMPs) is essential to maintain homeostasis in the skin. The aim of this study was to determine the concentration of metalloproteinase 2, tissue inhibitor of metalloproteinase 3 and the concentration of collagen type 1 in unwounded skin of diabetes type 1 and 2 and healthy controls. The treatment of diabetes resulted in a significant decrease of MMP2, increase of TIMP3 and COL1 concentrations in the skin as compared to the untreated diabetic skin. The concentrations of MMP2 in the skin of treated rats did not show significant differences from the healthy control group. TIMP3 concentrations in the skin of treated rats are not returned to the level observed in the control group. Disturbances of the extracellular matrix of the skin are similar in diabetes type 1 and 2. Application of insulin in diabetes therapy more preferably affects the extracellular matrix homeostasis of the skin.

scholarly journals LEVEL OF MARKERS OF NEURODEGENERATION IN PATIENTS WITH OCULAR ISCHEMIC SYNDROME

2014 ◽  
Vol 7 (2) ◽  
Author(s):  
Halidjan Kamilov ◽  
Munirahon Kasimova ◽  
Dilbar Makhkamova
Keyword(s):  
Blood Serum ◽  
Protein S ◽  
Main Group ◽  
Tear Fluid ◽  
Control Group ◽  
Normal Range ◽  
Ocular Ischemic Syndrome ◽  
S 100 ◽  
Blood Serum Protein ◽  
Ischemic Syndrome

The purpose of this study was to investigate the content neurospecific markers protein S-100 and neuroenolaza in blood serum and tear fluid of patients with ocular ischemic syndrome.Material and methods. We observed 43 patients aged 57 to 79 years, mean age 67.3 ± 2.7 years. Control group consisted of 11 volunteers  without ophthalmic symptoms. The main group consisted of 32 patients with OIS. The neurospecific proteins S100 and NSE were investigated in blood serum and tear fluid.Results. The study found that in patients of the control group the content of protein were within the normal range: S- 100 in the tear fluid – 0,0662 ± 0,00335 mkg/l, in the blood serum 0,0508 ± 0,00241 mkg/l. In patients of the main group the indicators of protein in the tear fluid were elevated in all patients - 3,12 ± 0,246 mkg/l ( p<0.005). The normal levels in blood serum of marker S-100 was in 30 patients - 0,0589 ± 0,00303 mkg/l, while, in 2 patients protein S-100 were raised and averaged 0,2175±0,00725 mkg/l. It was found that in patients of the control group content of protein NSE in the tear fluid and blood serum were within normal values - 15,86 ± 0,148 Ng/ml, 15,60 ± 0,202 Ng/ml respectively. In the main group the amount of protein NSE tended to increase in the tear fluid in 23 patients and averaged 33,012 ± 3,2626 Ng/ml (p<0.005), a significant decrease the quantity of protein was observed in 9 patients, which amounted to 5,166 ± 0,8301 Ng/ml. At normal levels in the blood serum protein NSE detected in 30 patients and averaged 14,48 ± 0,263 Ng/ml, whereas, in 2 patients there was a significant increase of content of protein NSE and was 27,47 ± 3,068 Ng/ml.Conclusions. Thus, changes in the concentration of S100 and neuroenolaza in the tear fluid in patients with ocular ischemic syndrome allow to identify as marker of nerve cells damage of the eye, contributing to the definition in conjunction with other signs of stage and etiology of the disease.

scholarly journals Impact of metronidazole and amoxicillin combination on matrix metalloproteinases-1 and tissue inhibitors of matrix metalloproteinases balance in generalized aggressive periodontitis

2015 ◽  
Vol 09 (01) ◽  
pp. 053-059 ◽  
Author(s):  
Emine Cifcibasi ◽  
Alpdogan Kantarci ◽  
Selim Badur ◽  
Halim Issever ◽  
Serdar Cintan
Keyword(s):  
Matrix Metalloproteinases ◽  
Alveolar Bone ◽  
Statistical Significance ◽  
Bone Destruction ◽  
Test Group ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Tissue Inhibitors ◽  
Clinical Healing ◽  
Concentration Levels

ABSTRACT Objective: Generalized aggressive periodontitis (GAgP) is a complex periodontal disease affecting the entire dentition with a rapid destruction of the periodontium and resulting in loss of teeth. We hypothesized that better clinical healing of adjunctive use of amoxicillin plus metronidazole combination may be related to the effect of this combination therapy to restore imbalance between matrix metalloproteinases (MMP) and their tissue inhibitors (TIMP) which is associated with connective tissue and alveolar bone destruction in patients with GAgP. Materials and Methods: Twenty-eight subjects diagnosed with GAgP were recruited. Patients were randomly assigned to test or control groups. MMP-1/TIMP-1 ratio was compared between groups receiving scaling and root planning (SRP) alone (control) or in combination with amoxicillin plus metronidazole (test). Clinical periodontal variables were measured. Gingival crevicular fluid samples were obtained and analyzed for MMP-1 and TIMP-1. Measurements were taken at baseline and repeated at 3 and 6 months after therapy. Results: Total MMP-1 levels were significantly decreased in both groups (P < 0.05) at 3 and 6 months. MMP-1 concentration levels showed a similar pattern to MMP-1 total levels decreasing significantly at 3 months (P < 0.05). TIMP-1 concentration levels increased in the test group throughout the study period, while the difference did not reach statistical significance (P > 0.05). TIMP-1/MMP-1 balance was restored in test group at 6 months significantly better than the control group (P < 0.05). Conclusion: The results of this study suggest that metronidazole and amoxicillin combination as an adjunct to SRP results in better clinical healing through restoring TIMP-1/MMP-1 balance.

scholarly journals Diagnostic Criteria for Acute Destructive Pancreatitis

2021 ◽  
Vol 6 (2) ◽  
pp. 94-100
Author(s):  
V. M. Likhman ◽  
◽  
A. A. Merkulov ◽  
A. N. Shevchenko ◽  
S. V. Tkach ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Blood Serum ◽  
Acute Phase ◽  
Inflammatory Process ◽  
Acute Phase Proteins ◽  
Proteolytic Enzymes ◽  
The Body ◽  
Control Group ◽  
Antibacterial Prophylaxis ◽  
Surgical Methods

The relevance of the emergence of innovative technologies, more sophisticated diagnostics methods, opportunities for intensive therapy, antibacterial prophylaxis, surgical methods of treatment involving minimally invasive surgery, does not solve the problem of high overall mortality in severe acute pancreatitis at this time. This indicator for the last ten years remains at a high level (10-30%), and in case of inflammatory forms of severe pancreatitis, reaches 85%. Traditional laboratory criteria for the diagnosis of pancreatic necrosis may involve leukocytosis with the destruction of the leukocyte formula and appearance of myelocytes and metamylocytes in it; improvement of leukocyte index of intoxication; improvement of the nuclear index of the destruction; blood glucose growth; growth of blood urea; growth of neutrophil-lymphocytic coefficient; change in endogenous creatinine clearance; improvement of blood transaminases. The indicator enzymes have the greatest value for the diagnosis of gastrointestinal pancreatitis in the diagnostic plan. The determining of α-amylase activity is a sensitive and valuable criterion for diagnostics of gastrointestinal pancreatitis and control over the effectiveness of treatment. Also, talking about laboratory diagnostics of acute pancreatitis it is worth noting about acute-phase proteins. The increase in the level of acute-phase proteins in inflammation is a compensatory reaction associated with the ability to inhibit cells released during destruction and cause secondary tissue damage by proteolytic enzymes, as well as to suppress autoimmune aggression. Due to this, the inflammatory process in the body is limited and autoaggression is restrained. The purpose of the study was to study the proteins of the acute phase in the blood of patients with acute pancreatitis of varying severity. Materials and methods. The study is based on the results of examination of 88 patients with acute pancreatitis of varying severity who were treated at the V.T. Zaytsev Institute of the General and Urgent Surgery of Academy of Medical Sciences of Ukraine, Kharkiv, as well as 45 donors (control group). Results and discussion. The determination of the activity of serum enzymes in the dynamics of acute destructive pancreatitis was carried out. The parameters were verified before the operation and after the operative treatment in dynamics, as well as before and after the second operation in the dynamics. Markers of tissue destruction can simultaneously serve as indicators of the severity of the inflammatory process in the pancreas and determine the quality of the performed surgical intervention. The diagnostic value of ferritin and lactofferin as markers of an acute destructive process was proved on the basis of a significant increase in their level in blood serum and exudates of patients. It has been shown that ferroproteins are reliable markers of the inflammatory process, among others, including classical acute phase proteins. At the same time, their identification is distinguished by its explicitness in the diagnosis of not only latent destructive states. Conclusion. The detected changes in the concentration of clinical and laboratory parameters of alpha-2-macroglobulin, lactoferrin, C-reactive protein, ferritin in the blood serum correlate with the degree of the inflammatory process and purulent-destructive changes in the pancreas

Matrix metalloproteinases and their tissue inhibitors in relapsing remitting multiple sclerosis : Possible markers and treatment agents

2021 ◽  
Vol 74 (1-2) ◽  
pp. 50-56
Author(s):  
Arzu Sanli ◽  
Musa Ozturk ◽  
Aysun Soysal ◽  
Yasemin Doventas ◽  
Fulya Basoglu ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Matrix Metalloproteinases ◽  
Patient Group ◽  
Healthy Subjects ◽  
Early Period ◽  
Control Group ◽  
Tissue Inhibitors ◽  
Remission Period ◽  
Significant Difference ◽  
Relapsing Remitting

Matrix metalloproteinases (MMPs), which are synthesized by many cell groups and responsible for the destruction of matrix proteins, and endogen tissue inhibitors of MMPs (TIMPs) have a role in the pathogenesis of Multiple Sclerosis (MS) by affecting the blood-brain barrier. We aimed to investigate the role of MMPs and TIMPs in the immunopathogenesis and in the course of multiple sclerosis (MS). We enrolled 25 relapsing remitting MS patients, who had a definite MS diagnosis according to McDonald criteria and 25 healthy subjects similar for age and gender as control group. MMP-9- and TIMP-1 levels were measured twice in patient group (one time during an attack and one in remission) and once in healthy subjects. MMP-9- and TIMP-levels of patients during attack and remission period and MMP-9/TIMP-1 ratio were found significantly higher than in the control subjects. In patient group MMP-9 and TIMP-1 levels and MMP-9/TIMP-1 ratio during attacks were not significantly different than during remission period. However, when subdivided according to their number of attacks, patients with 2 attacks had significantly higher levels during attack period comparing to remission period (p<0.05); in case of patients with more than 2 attacks did not have a statistically significant difference in attack and remission periods. Matrix metalloproteinases are important actors in MS immunopathogenesis, particularly in the early period and inhibitor agents for these enzymes can be used as a treatment option.

scholarly journals Clinical prospects of matrix metalloproteinases and their tissue inhibitors study in blood serum of patients with endometrial cancer and benign endometrial lesions

2017 ◽  
Vol 45 (4) ◽  
pp. 280-288
Author(s):  
E. S. Gershtein ◽  
S. V. Mushtenko ◽  
R. E. Kuznetsov ◽  
V. D. Ermilova ◽  
N. Ye. Levchenko ◽  
...  
Keyword(s):  
Endometrial Cancer ◽  
Matrix Metalloproteinases ◽  
Blood Serum ◽  
Tissue Inhibitors

scholarly journals Monocyte chemotactic factor content in normal sperm and in samples of ejaculate with diminished fertility

2019 ◽  
Vol 36 (3) ◽  
pp. 28-37
Author(s):  
Dmitriy Yu. Sosnin ◽  
Konstantin R. Galkovich
Keyword(s):  
Blood Serum ◽  
Seminal Plasma ◽  
Solid Phase ◽  
Reproductive Organs ◽  
Chemotactic Factor ◽  
Control Group ◽  
Mean Values ◽  
Healthy Men ◽  
Significant Difference ◽  
The Mean

Aim. To study the dynamics of monocyte chemotactic factor (MCP-1) content in the ejaculate of healthy men and men with diminished spermatozoid concentration. Material and methods.Sixty-four men were examined. The main group included 16 patients with azoospermia. The comparison group – 24 patients with oligozooasthenospermia (spermatozoid concentration lower than 15 mln/ml). The control group was presented by ejaculate samples of 24 healthy men, characterized by normal parameters. The MCP-1 concentration was determined with the method of solid phase enzyme immunoassay using the assay kit “MCP-1-EIA-BEST” (A8784) (CJSC Vector-Best, Russia). Results. The mean MCP-1 concentration in the samples of undiluted seminal plasma was 2242.8 ± 672.0 pg/ml versus the blood serum, where MCP-1 content was 18.9 times lower and was 118.8 ± 22.9 pg/ml. No reliable differences were revealed when comparing the mean values of MCP-1 in the studied groups in ejaculates; there were also no statistically significant difference between the indices in MCP-1 groups in the blood serum. Conclusions. Male seminal plasma is characterized by unusually high MCP-1 content, exceeding more than tenfold the concentration of this protein in the blood serum. High MCP-1 concentration in sperm and the absence of its dependence on the concentration of this protein in blood serum indicates local production of this protein into the seminal plasma by male reproductive organs. Further studies are needed to find out a concrete localization of the sites of production of this protein in male genital organs and to study its probable role in reproductive processes in male and female organisms.

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