9. Imaging the distributions of lipids and proteins in the plasma membrane with high-resolution secondary ion mass spectrometry

2019 ◽  
pp. 287-322 ◽  
Keyword(s):  
Mass Spectrometry ◽  
Plasma Membrane ◽  
High Resolution ◽  
Secondary Ion Mass Spectrometry ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Imaging and 3D Elemental Characterization of Intact Bacterial Spores by High-Resolution Secondary Ion Mass Spectrometry

2008 ◽  
Vol 80 (15) ◽  
pp. 5986-5992 ◽  
Author(s):  
Sutapa Ghosal ◽  
Stewart J. Fallon ◽  
Terrance J. Leighton ◽  
Katherine E. Wheeler ◽  
Michael J. Kristo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Ion Mass Spectrometry ◽  
Bacterial Spores ◽  
Ion Mass Spectrometry ◽  
Secondary Ion ◽  
Elemental Characterization

Localization of iron in rice grain using synchrotron X-ray fluorescence microscopy and high resolution secondary ion mass spectrometry

2014 ◽  
Vol 59 (2) ◽  
pp. 173-180 ◽  
Author(s):  
Bianca Kyriacou ◽  
Katie L. Moore ◽  
David Paterson ◽  
Martin D. de Jonge ◽  
Daryl L. Howard ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Fluorescence Microscopy ◽  
Secondary Ion Mass Spectrometry ◽  
Rice Grain ◽  
X Ray ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

scholarly journals Sphingolipid Domains in the Plasma Membranes of Fibroblasts Are Not Enriched with Cholesterol

2013 ◽  
Vol 288 (23) ◽  
pp. 16855-16861 ◽  
Author(s):  
Jessica F. Frisz ◽  
Haley A. Klitzing ◽  
Kaiyan Lou ◽  
Ian D. Hutcheon ◽  
Peter K. Weber ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Plasma Membrane ◽  
High Resolution ◽  
Mammalian Cells ◽  
Plasma Membranes ◽  
Secondary Ion Mass Spectrometry ◽  
Cholesterol Depletion ◽  
Fibroblast Cells ◽  
Secondary Ion ◽  
Rule Out

The plasma membranes of mammalian cells are widely expected to contain domains that are enriched with cholesterol and sphingolipids. In this work, we have used high-resolution secondary ion mass spectrometry to directly map the distributions of isotope-labeled cholesterol and sphingolipids in the plasma membranes of intact fibroblast cells. Although acute cholesterol depletion reduced sphingolipid domain abundance, cholesterol was evenly distributed throughout the plasma membrane and was not enriched within the sphingolipid domains. Thus, we rule out favorable cholesterol-sphingolipid interactions as dictating plasma membrane organization in fibroblast cells. Because the sphingolipid domains are disrupted by drugs that depolymerize the cells actin cytoskeleton, cholesterol must instead affect the sphingolipid organization via an indirect mechanism that involves the cytoskeleton.

Silica and Magnesia Dopant Distributions in Alumina by High-Resolution Scanning Secondary Ion Mass Spectrometry

1999 ◽  
Vol 82 (4) ◽  
pp. 1001-1008 ◽  
Author(s):  
Konstantin L. Gavrilov ◽  
Stephen J. Bennison ◽  
Kurt R. Mikeska ◽  
Jan M. Chabala ◽  
Riccardo Levi-Setti
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Secondary Ion Mass Spectrometry ◽  
Ion Mass Spectrometry ◽  
Secondary Ion
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