Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

AbstractData on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2= 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2= 0.118), as well as age (p = 0.073, r2= 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.

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Assessment of Antioxidant Status and Lipid Peroxidation in Pre-Eclampsia

Journal of Advances in Medical and Pharmaceutical Sciences ◽

10.9734/jamps/2019/v21i430138 ◽

2019 ◽

pp. 1-9

Author(s):

Patrick O. Manafa ◽

Charles C. Onyenekwe ◽

Anselem C. Igwe ◽

Nancy A. Mbachu ◽

George O. Chukwuma ◽

...

Keyword(s):

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Glutathione Peroxidase ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Positive Correlation ◽

Spectrophotometric Methods ◽

Significant Difference ◽

Total Antioxidant ◽

Blood Pressures

Aim: To assess the role of antioxidants and lipid peroxidation in the pathogenesis of pre-eclampsia. Study Design: This is a case-control study designed to evaluate the levels of malondialdehyde and total antioxidant capacity with glutathione peroxidase and superoxide dismutase activities in pre-eclamptic. One hundred (100) participants were randomly selected to include fifty pre-eclamptic (test), twenty-five (25) healthy pregnant normotensive women (control A) and twenty-five healthy non-pregnant normotensive women (control B). Place and Duration of Study: This study was carried out at the Holy Rosary Hospital waterside, Onitsha, Anambra state. Nigeria. The study lasted for 13 months. Methodology: One hundred women (50 pre-eclamptic, 25 pregnant normotensives and 25 non-pregnant normotensives) aged 25-40 years were recruited for this study. Blood samples were collected from the participants for the estimation of total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities using spectrophotometric methods. Blood pressures were measured using accosson sphygmomanometer. Data were analysed using analysis of variance and Pearson’s correlation coefficient at p < 0.05. Results: The TAC was significantly higher in the pregnant normotensives (p< 0.05) compared with the pre-eclamptic and non-pregnant normotensives. There was no significant difference in the MDA in pre-eclamptic compared with pregnant normotensives and non-pregnant normotensives (p>0.05). A significant difference was observed in the SOD and GPx activities in the pregnant normotensive and non-pregnant normotensives compared with pre-eclamptic. A positive correlation was observed between the blood pressures (systolic and diastolic) and the activities of TAC, SOD and GPx in pre-eclamptic. Furthermore, a positive correlation was observed between MDA levels and SBP (r = 0.019) while a negative correlation (r = -0.225) existed between MDA and DBP. Conclusion: Diminished ability of antioxidants to scavenge free radicals may affect the onset of pre-eclampsia and therefore possible prognostic tool in its management.

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Effect of EPA and Vitamin C on Superoxide Dismutase, Glutathione Peroxidase, Total Antioxidant Capacity and Malondialdehyde in Type 2 Diabetic Patients

Oman Medical Journal ◽

10.5001/omj.2014.09 ◽

2014 ◽

Vol 29 (1) ◽

pp. 39-45 ◽

Cited By ~ 12

Author(s):

Mohammad Mehdi Shakouri Mahmoudabadi ◽

Ali Reza Rahbar

Keyword(s):

Superoxide Dismutase ◽

Vitamin C ◽

Glutathione Peroxidase ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Diabetic Patients ◽

Type 2 Diabetic Patients ◽

Total Antioxidant ◽

Type 2 Diabetic

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Antioxidative responses to seasonal changes and chemiluminescence assay of Astragalus onobrychis leaves extract

Open Chemistry ◽

10.2478/s11532-012-0163-6 ◽

2013 ◽

Vol 11 (2) ◽

pp. 123-132

Author(s):

Dragoljub Miladinović ◽

Budimir Ilić ◽

Stevo Najman ◽

Olga Cvetković ◽

Aleksandra Šajnović ◽

...

Keyword(s):

Superoxide Dismutase ◽

Enzyme Activity ◽

Glutathione Peroxidase ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Seasonal Changes ◽

Antioxidant Enzyme Activity ◽

Guaiacol Peroxidase ◽

Stages Of Growth ◽

Total Antioxidant

AbstractThe aim of this study was to research the seasonal changes of antioxidant enzyme activity and total antioxidant capacity in leaves of Astragalus onobrychis L. subsp. chlorocarpus (Griseb.) S. Kozuharov et D.K. Pavlova. Leaves of A. onobrychis were collected during the different stages of growth and analyzed for antioxidant enzyme activity: superoxide dismutase, catalase, guaiacol peroxidase, glutathione peroxidase. Quantities of malonyldialdehyde, superoxide radicals, and hydroxyl radicals were measured as well as the content of soluble proteins. Furthermore, total antioxidant capacity was determined by the inhibition of chemiluminescence activity of blood phagocytes by leaf extracts. Stages of vegetation significantly affected the accumulation of superoxide radicals, but there were no significant differences in hydroxyl radical quantity and lipid peroxidation levels during vegetation. Soluble proteins vary greatly between different stages of growth. Seasonal changes were found to have an effect on enzymatic activities. During the spring season, guaiacol peroxidase showed the highest levels. Catalase and glutathione peroxidase increased their activities in summer, while, during the autumn season, superoxide dismutase showed maximum activity. On the basis of chemiluminescence assay, it can be concluded that leaf extract of A. onobrychis possesses a significant antioxidant capacity thus protecting plants during environmental stress.

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Antioxidant status of rabbits after treatment with epicatechin and patulin

Biologia ◽

10.1515/biolog-2016-0098 ◽

2016 ◽

Vol 71 (7) ◽

Cited By ~ 4

Author(s):

Katarina Zbynovska ◽

Peter Petruska ◽

Anna Kalafova ◽

Lubomir Ondruska ◽

Rastislav Jurcik ◽

...

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Antioxidant Status ◽

Total Antioxidant ◽

Antioxidant Parameters

AbstractThe aim of this study was to assess antioxidant status by measuring of antioxidant parameters (SOD – superoxide dismutase, GPx – glutatione peroxidase, CAT – catalase, Trolox, TAC – total antioxidant capacity, bilirubin, albumin) and parameters associated with oxidative stress (MDA – malondialdehyde, MDA Adduct – malondialdehyde adduct, H

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Silibinin and Nano-Silibinin in Cuprizone Model of Multiple Sclerosis: Behavioral and Biochemical Investigation

Current Drug Therapy ◽

10.2174/1574885517666211216161317 ◽

2021 ◽

Vol 17 ◽

Author(s):

Yasin Ahmadi ◽

Mohammad Mehdi Mahboobian ◽

Mojdeh Mohammadi

Keyword(s):

Oxidative Stress ◽

Multiple Sclerosis ◽

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Antioxidant Capacity ◽

Open Field ◽

Total Antioxidant Capacity ◽

Tail Flick ◽

Total Antioxidant ◽

Antioxidant Parameters

Objectives: Multiple sclerosis (MS) is a long-lasting demyelinating inflammatory disease of the central nervous system (CNS). It has been shown that brain tissue in MS is exposed to oxidative stress during the disease period. Silymarin, a plant-derived flavonoid, can be extracted from Silybum marianum. The current experiment aimed to explore the effects of silibinin and especially nano-silibinin on neurobehavioral activity and biochemical antioxidant parameters in the cuprizone model of demyelination in mice for the first time. Methods: Demyelination was induced in mice by oral consumption of cuprizone 0.4%w/w for one week and then 0.2%w/w for four weeks. Treatment was performed with silibinin or nano-silibinin (70mg/kg body weight) for four weeks at the same time with cuprizone 0.2%w/w. After neurobehavioral tests (rotarod, tail flick, and open field), biochemical antioxidant parameters (glutathione level, superoxide dismutase activity, lipid peroxidation products, and total antioxidant capacity) were evaluated. Results: In this experiment, behavioral tests (rotarod and open field) displayed improvement in movement dysfunction using silibinin or nano-silibinin. Furthermore, silibinin and more efficiently nano-silibinin increased antioxidant parameters, such as superoxide dismutase (SOD) and glutathione (GSH) and total antioxidant capacity (TAC), and decreased lipid peroxidation. Conclusion: These data suggest that silibinin and nano-silibinin can improve movements in the cuprizone model of demyelination. Moreover, they may prevent cuprizone-induced oxidative stress. In conclusion, silibinin and more effectively, nano-silibinin, may exhibit therapeutic features in MS disease.

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The role of oxidants and antioxidative parameters in patients with hepatic encephalopathy

International Journal of Scientific Reports ◽

10.18203/issn.2454-2156.intjscirep20212838 ◽

2021 ◽

Vol 7 (8) ◽

pp. 388

Author(s):

Sami Bahcebasi ◽

Gulden Baskol ◽

Mevult Baskol

Keyword(s):

Nitric Oxide ◽

Superoxide Dismutase ◽

Hepatic Encephalopathy ◽

Glutathione Peroxidase ◽

Antioxidant Capacity ◽

Xanthine Oxidase ◽

Total Antioxidant Capacity ◽

Control Group ◽

Total Antioxidant ◽

Oxidative System

Background: Hepatic encephalopathy is a serious neuropsychiatric complication of cirrhosis. Changes in the oxidative and anti-oxidative system and nitric oxide levels in brain tissue contribute to the development of symptoms related to HE and HE. Purpose of the study to reveal the alterations in oxidative, anti-oxidative system and nitric oxide levels in cirrhotic patients during and after hepatic encephalopathy periods.Methods: This was a randomized controlled double-blind study conducted in Erciyes University Hospital between 3 July 2010 and 30 March 2011. We investigated the oxidative and anti-oxidative stress parameters by quantification of total antioxidant capacity (TAC), total oxidant capacity (TOC), nitric oxide (NO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total thiol and xanthine oxidase (XO) levels in serum. We compared the group of patients with hepatic encephalopathy, post-hepatic encephalopathy (clinically recovered) and control groups (healthy control). Results: Thirty hepatic encephalopathy patients were studied. Serum levels of nitric oxide and xanthine oxidase were statistically significantly high in the hepatic encephalopathy group according to control group (p<0.031, and p<0.001, respectively). Serum thiol levels were significantly low in hepatic encephalopathy patients than the controls (p<0.001). Total oxidant capacity, total antioxidant capacity, glutathione peroxidase and superoxide dismutase levels were not significantly different in hepatic encephalopathy group than the controls. Serum thiol levels were low and serum NO levels were high in recovered clinically from hepatic encephalopathy group according to control group currently (p<0.001, p<0.001, respectively). Total antioxidant capacity, total oxidant capacity, glutathione peroxidase, superoxide dismutase and xanthine oxidase levels were similar in both groups (p>0.05). Total antioxidant capacity and especially xanthine oxidase levels were significantly decreased in recovered clinically from hepatic encephalopathy group compared to hepatic encephalopathy group (p<0.05, p<0.001, respectively).Conclusions: Oxidative system, in systemic circulation, is activated during hepatic encephalopathy and changes in XO level during and after hepatic encephalopathy is very different. This parameter may be a potential marker in differential diagnosis of hepatic encephalopathy from other coma causes. Further investigation is needed.

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