Silibinin and Nano-Silibinin in Cuprizone Model of Multiple Sclerosis: Behavioral and Biochemical Investigation

2021 ◽  
Vol 17 ◽  
Author(s):  
Yasin Ahmadi ◽  
Mohammad Mehdi Mahboobian ◽  
Mojdeh Mohammadi
Keyword(s):  
Oxidative Stress ◽  
Multiple Sclerosis ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Open Field ◽  
Total Antioxidant Capacity ◽  
Tail Flick ◽  
Total Antioxidant ◽  
Antioxidant Parameters

Objectives: Multiple sclerosis (MS) is a long-lasting demyelinating inflammatory disease of the central nervous system (CNS). It has been shown that brain tissue in MS is exposed to oxidative stress during the disease period. Silymarin, a plant-derived flavonoid, can be extracted from Silybum marianum. The current experiment aimed to explore the effects of silibinin and especially nano-silibinin on neurobehavioral activity and biochemical antioxidant parameters in the cuprizone model of demyelination in mice for the first time. Methods: Demyelination was induced in mice by oral consumption of cuprizone 0.4%w/w for one week and then 0.2%w/w for four weeks. Treatment was performed with silibinin or nano-silibinin (70mg/kg body weight) for four weeks at the same time with cuprizone 0.2%w/w. After neurobehavioral tests (rotarod, tail flick, and open field), biochemical antioxidant parameters (glutathione level, superoxide dismutase activity, lipid peroxidation products, and total antioxidant capacity) were evaluated. Results: In this experiment, behavioral tests (rotarod and open field) displayed improvement in movement dysfunction using silibinin or nano-silibinin. Furthermore, silibinin and more efficiently nano-silibinin increased antioxidant parameters, such as superoxide dismutase (SOD) and glutathione (GSH) and total antioxidant capacity (TAC), and decreased lipid peroxidation. Conclusion: These data suggest that silibinin and nano-silibinin can improve movements in the cuprizone model of demyelination. Moreover, they may prevent cuprizone-induced oxidative stress. In conclusion, silibinin and more effectively, nano-silibinin, may exhibit therapeutic features in MS disease.

Antioxidant status of rabbits after treatment with epicatechin and patulin

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Katarina Zbynovska ◽  
Peter Petruska ◽  
Anna Kalafova ◽  
Lubomir Ondruska ◽  
Rastislav Jurcik ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antioxidant Status ◽  
Total Antioxidant ◽  
Antioxidant Parameters

AbstractThe aim of this study was to assess antioxidant status by measuring of antioxidant parameters (SOD – superoxide dismutase, GPx – glutatione peroxidase, CAT – catalase, Trolox, TAC – total antioxidant capacity, bilirubin, albumin) and parameters associated with oxidative stress (MDA – malondialdehyde, MDA Adduct – malondialdehyde adduct, H

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

scholarly journals Decreased Superoxide Dismutase Concentrations (SOD) in Plasma and CSF and Increased Circulating Total Antioxidant Capacity (TAC) Are Associated with Unfavorable Neurological Outcome after Aneurysmal Subarachnoid Hemorrhage

2021 ◽  
Vol 10 (6) ◽  
pp. 1188
Author(s):  
Harald Krenzlin ◽  
Dominik Wesp ◽  
Jan Schmitt ◽  
Christina Frenz ◽  
Elena Kurz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Antioxidant Capacity ◽  
Neurological Outcome ◽  
Total Antioxidant Capacity ◽  
Early Brain Injury ◽  
Fisher Grade ◽  
Total Antioxidant

Background: Subarachnoid hemorrhage (SAH) is a devastating disease with high morbidity and mortality. Hypoxia-induced changes and hemoglobin accumulation within the subarachnoid space are thought to lead to oxidative stress, early brain injury, and delayed vasospasm. This study aimed to evaluate the antioxidant status and its impact on neurological outcome in patients with aneurysmal SAH. Methods: In this prospective observational study, 29 patients with aneurysmal SAH were included (mean age 54.7 ± 12.4). Blood and cerebrospinal fluid (CSF) samples were collected on days (d) 1, 3, and 7. In addition, 29 patients without intracranial hemorrhage served as controls. The antioxidant system was analyzed by glutathione peroxidase (GSH-Px; U/L) and total and free glutathione-sulfhydryl (GSH; mg/L) in the plasma. Superoxide dismutase (SOD, U/mL) and total antioxidant capacity (TAC, µmol/L) were measured in the serum and CSF. Clinical data were compiled on admission (Hunt and Hess grade, Fisher grade, and GCS). Neurological and cognitive outcome (modified Rankin scale (mRS), Glasgow Outcome Scale Extended (GOSE) and Montreal Cognitive Assessment (MoCA)) was assessed after 6 weeks (6 w) and 6 months (6 m). Results: Plasma levels of SOD increased from day 1 to 7 after SAH (d1: 1.22 ± 0.36 U/L; d3: 1.25 ± 0.33 U/L, p = 0.99; d7: 1.52 ± 0.4 U/L, p = 0.019) and were significantly higher compared to controls (1.11 ± 0.27 U/L) at day 7 (p < 0.001). Concordantly, CSF levels of SOD increased from day 1 to 7 after SAH (d1: 1.22 ± 0.41 U/L; d3: 1.77 ± 0.73 U/L, p = 0.10; d7: 2.37 ± 1.29 U/L, p < 0.0001) without becoming significantly different compared to controls (1.74 ± 0.8 U/L, p = 0.09). Mean plasma TAC at day 1 (d1: 77.87 ± 49.72 µmol/L) was not statistically different compared to controls (46.74 ± 32.42 µmol/L, p = 0.25). TAC remained unchanged from day 1 to 7 (d3: 92.64 ± 68.58 µmol/L, p = 0.86; d7: 74.07 ± 54.95 µmol/L, p = 0.8) in plasma. TAC in CSF steeply declined from day 1 to 7 in patients with SAH becoming significantly different from controls at days 3 and 7 (d3: 177.3 ± 108.7 µmol/L, p = 0.0046; d7: 85.35 ± 103.9 µmol/L, p < 0.0001). Decreased SOD levels in plasma and CSF are associated with a worse neurological outcome 6 weeks (mRS: CSF p = 0.0001; plasma p = 0.027/GOSE: CSF p = 0.001; plasma p = 0.001) and 6 months (mRS: CSF p = 0.001; plasma p = 0.09/GOSE: CSF p = 0.001; plasma p = 0.001) after SAH. Increased plasma TAC correlated with a worse neurological outcome 6 weeks (mRS: p = 0.001/GOSE p = 0.001) and 6 months (mRS p = 0.001/GOSE p = 0.001) after SAH. Conclusion: In our study, a reduction in the antioxidative enzyme SOD and elevated TAC were associated with a poorer neurological outcome reflected by mRS and GOSE at 6 weeks and 6 months after SAH. A lower initial SOD CSF concentration was associated with the late deterioration of cognitive ability. These findings support the mounting evidence of the role of oxidative stress in early brain injury formation and unfavorable outcome after SAH.

Oxidative stress in operating room personnel: occupational exposure to anesthetic gases

2005 ◽  
Vol 24 (11) ◽  
pp. 597-601 ◽  
Author(s):  
A Akbar Malekirad ◽  
A Ranjbar ◽  
K Rahzani ◽  
M Kadkhodaee ◽  
A Rezaie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Operating Room ◽  
Total Antioxidant Capacity ◽  
Plasma Lipid ◽  
The Body ◽  
Operating Room Personnel ◽  
Total Antioxidant ◽  
Anesthetic Gases

Health professionals exposed to anesthetic gases are at higher risk of reproductive, neurological, hematological, immunological, hepatic and renal system diseases. We investigated if oxidative stress induced by chronic exposure to anesthetic gases has any association with this matter. Plasma lipid peroxidation, total antioxidant capacity and total thiol molecule levels were measured in 66 operating room staff in comparison with 66 controls. The exposed group had a significantly higher level of lipid peroxidation with decreased thiol groups compared to control subjects. Total antioxidant capacity of the body was no different among exposed and not exposed subjects. Increased lipid peroxidation in the blood of exposed subjects warns that oxygen free radicals have increased in the body and thus might attack cells, which, in the longterm, results in multi-organ damage. The remaining blood total antioxidant capacity at normal values is promising and means that other non-thiol antioxidants, such as uric acid, transferrin, ceruloplasmin, albumin, and vitamin antioxidants, such as a-tocopherol and ascorbic acid, have been stimulated to maintain the total anti-oxidant power of the body at normal state.

scholarly journals Plasma Total Antioxidant Capacity and Activities of Blood Glutathione Peroxidase and Superoxide Dismutase Determined in Healthy Dogs by Using Commercially Available Kits

2016 ◽  
Vol 66 (4) ◽  
pp. 534-548 ◽  
Author(s):  
Katerina Tomsič ◽  
Alenka Seliškar ◽  
Barbara Lukanc ◽  
Alenka Nemec Svete
Keyword(s):  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Significant Difference ◽  
Erythrocyte Superoxide Dismutase ◽  
Total Antioxidant ◽  
Antioxidant Parameters ◽  
Healthy Dogs ◽  
Effect Of Age

AbstractData on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2= 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2= 0.118), as well as age (p = 0.073, r2= 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.

scholarly journals Assessment of Antioxidant Status and Lipid Peroxidation in Pre-Eclampsia

2019 ◽  
pp. 1-9
Author(s):  
Patrick O. Manafa ◽  
Charles C. Onyenekwe ◽  
Anselem C. Igwe ◽  
Nancy A. Mbachu ◽  
George O. Chukwuma ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Positive Correlation ◽  
Spectrophotometric Methods ◽  
Significant Difference ◽  
Total Antioxidant ◽  
Blood Pressures

Aim: To assess the role of antioxidants and lipid peroxidation in the pathogenesis of pre-eclampsia. Study Design: This is a case-control study designed to evaluate the levels of malondialdehyde and total antioxidant capacity with glutathione peroxidase and superoxide dismutase activities in pre-eclamptic. One hundred (100) participants were randomly selected to include fifty pre-eclamptic (test), twenty-five (25) healthy pregnant normotensive women (control A) and twenty-five healthy non-pregnant normotensive women (control B). Place and Duration of Study: This study was carried out at the Holy Rosary Hospital waterside, Onitsha, Anambra state. Nigeria. The study lasted for 13 months. Methodology: One hundred women (50 pre-eclamptic, 25 pregnant normotensives and 25 non-pregnant normotensives) aged 25-40 years were recruited for this study. Blood samples were collected from the participants for the estimation of total antioxidant capacity (TAC), malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities using spectrophotometric methods. Blood pressures were measured using accosson sphygmomanometer. Data were analysed using analysis of variance and Pearson’s correlation coefficient at p < 0.05. Results: The TAC was significantly higher in the pregnant normotensives (p< 0.05) compared with the pre-eclamptic and non-pregnant normotensives. There was no significant difference in the MDA in pre-eclamptic compared with pregnant normotensives and non-pregnant normotensives (p>0.05). A significant difference was observed in the SOD and GPx activities in the pregnant normotensive and non-pregnant normotensives compared with pre-eclamptic. A positive correlation was observed between the blood pressures (systolic and diastolic) and the activities of TAC, SOD and GPx in pre-eclamptic. Furthermore, a positive correlation was observed between MDA levels and SBP (r = 0.019) while a negative correlation (r = -0.225) existed between MDA and DBP. Conclusion: Diminished ability of antioxidants to scavenge free radicals may affect the onset of pre-eclampsia and therefore possible prognostic tool in its management.

scholarly journals LIFE HABITS AND LIPID PEROXIDATION OF WOMEN OVER 40 YEARS OLD

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S148-S148
Author(s):  
Renata F Oliveira ◽  
Maria Mota ◽  
Jorge Soares ◽  
Zirlene Santos ◽  
Bianca Rosa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Catalase Activity ◽  
Total Antioxidant Capacity ◽  
Multiple Linear Regression Model ◽  
Activity Level ◽  
Leg Strength ◽  
Total Antioxidant

Abstract Oxidative stress is involved in degenerative processes, aging, and diseases. Lifestyle can be change oxidative stress. One of reactive oxygen species targets is polyunsaturated fatty acid, an important cellular membrane component. The aim of this study is to analyse the contribute of lifestyle in lipid peroxidation in over fourty years old women. Were included 60 women whith age ranged between 41 and 82 years old (53.3 ± 9.1 years). Lifestyle were explored doing a food frequency questionnaire, Perceptive Stress Scale (PSS) and International Physical Activity Questionnaire (IPAQ). The functional capacity was determinate by 6-min walking test (cardiorespiratory capacity) and Squat-jump (leg strength and power). Parameters of oxidative stress were determinate from plasma during fasting, lipid peroxidation was evaluated by TBARs, and antioxidant capacity was evaluated by catalase activity and ABTS. Spearman correlation and Multiple Linear Regression model, through Stepwise method, considering TBARs as dependent variable, and age, weight, body mass index, waist circumference, stress perception, physical activity level, total antioxidant capacity, catalase activity, cardiorespiratory capacity, leg strength and power, daily caloric intake, daily fruit, vegetables, coffee/tea, vitamin E and alcohol intake, as independent variable, was performed. Negative correlations were obtained between TBARs and cardiorespiratory capacity (r= -0.35; p=0.026) and between TBARS and ABTS (r= -0.33; p=0.038). Total antioxidant capacity was the model’s first variable (F= 5.50; p = 0.013), explaining 15.3% of TBARS, then cardiorespiratory capacity (F= 5.50; p = 0.047), explaining 10.5% of TBARs The results revealed total antioxidant capacity and cardiorespiratory capacity as predictors to lipid peroxidation damage.

scholarly journals Influence of Bone Marrow-Derived Mesenchymal Stem Cell Therapy on Oxidative Stress Intensity in Minimally Conscious State Patients

2020 ◽  
Vol 9 (3) ◽  
pp. 683
Author(s):  
Katarzyna Jezierska-Wozniak ◽  
Emilia Sinderewicz ◽  
Wioleta Czelejewska ◽  
Pawel Wojtacha ◽  
Monika Barczewska ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Marrow ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Minimally Conscious State ◽  
Conscious State ◽  
Total Antioxidant ◽  
Minimally Conscious

Neurological disorders, including minimally conscious state (MCS), may be associated with the presence of high concentrations of reactive oxygen species within the central nervous system. Regarding the documented role of mesenchymal stem cells (MSCs) in oxidative stress neutralization, the aim of this study is to evaluate the effect of bone marrow-derived MSC (BM-MSC) transplantation on selected markers of oxidative stress in MCS patients. Antioxidant capacity was measured in cerebrospinal fluid (CSF) and plasma collected from nine patients aged between 19 and 45 years, remaining in MCS for 3 to 14 months. Total antioxidant capacity, ascorbic acid and ascorbate concentrations, superoxide dismutase, catalase, and peroxidase activity were analyzed and the presence of tested antioxidants in the CSF and plasma was confirmed. Higher ascorbic acid (AA) content and catalase (CAT) activity were noted in CSF relative to plasma, whereas superoxide dismutase (SOD) activity and total antioxidant capacity were higher in plasma relative to CSF. Total antioxidant capacity measured in CSF was greater after BM-MSC transplantations. The content of ascorbates was lower and CAT activity was higher both in CSF and plasma after the administration of BM-MSC. The above results suggest that MSCs modulate oxidative stress intensity in MCS patients, mainly via ascorbates and CAT activity.

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