scholarly journals After bleaching enamel remineralization using a bioactive glass-ceramic (BioSilicate®)

2016 ◽  
Vol 2 (1) ◽  
Author(s):  
A. N. S. Rastelli ◽  
G. Nicolodelli ◽  
R. A. Romano ◽  
D. M. B. P. Milori ◽  
I. L. O. Perazzoli ◽  
...  

AbstractTooth bleaching agents may weaken the tooth structure, therefore, it is important to minimize any risks of enamel and dentine damage caused by them. In this way, different materials have been used to avoid or minimize the tooth damage during bleaching. Recently, bioactive glasses have been demonstrated to be effective in mineralization of dental structures. Therefore, this study evaluated the effect of BioSilicate® (a polycrystalline bioactive glass-ceramic) after bleaching by Laser-induced breakdown spectroscopy (LIBS) technique. Bovine dental blocks with 4 × 4 × 3 mm were obtained (n = 20), sequentially embedded in epoxy resin and then polished. Bleaching was performed using 35% hydrogen peroxide (Whiteness HP). Calcium (Ca) and phosphate (P) intensity values by LIBSwere obtained before the treatment (T0, baseline – control Group), after bleaching (T1), and after BioSilicate® application (T2). The use of BioSilicate® after bleaching showed to be an optimal way to remineralize enamel surface making BioSilicate® application a promising adjunct step to avoid or minimize the mineral loss on enamel surface after bleaching.

Author(s):  
Raíssa Manoel Garcia ◽  
Waldemir Francisco Vieira-Junior ◽  
Jéssica Dias Theobaldo ◽  
Flávio Henrique Baggio Aguiar ◽  
Débora Alves Nunes Leite Lima

Abstract Objectives The role of toothpaste used during aesthetic treatments is rarely investigated. The objective was to evaluate the effects of a bioactive glass-based toothpaste (BGT) used before or after the dental bleaching with 35% hydrogen peroxide (HP). Materials and Methods Bovine enamel blocks (4 × 4 mm; n = 12) were submitted to tooth bleaching and different treatments/storage, before or after HP, based on: (1) no toothpaste and immersion in artificial saliva (AS) after HP for 24 hours, 7 days, or 14 days (control); (2) daily BGT use and AS storage for 7 or 14 days (after HP); and (3) daily BGT use and AS storage for 7 or 14 days (prior to HP). Surface and in-depth color were determined using the CIE L*a*b* system (ΔL*, Δa*, Δb*, and ΔE) on enamel surface and underlying dentin. The surface microhardness (SMH) was evaluated using a Knoop microhardness tester. Statistical Analysis Data were submitted to analysis of variance (ANOVA) (color variables), repeated measures ANOVA (SMH), and Tukey’s test (α = 0.05). Results The color changes on the enamel or underlying dentin were not statistically different among the groups (p > 0.05). Twenty-four hours after HP presented a decrease in SMH differing from baseline (p < 0.01). This decrease did not occur in the groups previously exposed to BGT (p > 0.05). BGT use after HP for 7 days differed from group with exclusive AS storage (p < 0.05). Conclusion In-office tooth bleaching can decrease the microhardness of enamel surface; however, the use of BGT promotes the protection or enables the mineral recovery of tooth without the influence the bleaching efficacy.


2020 ◽  
Vol 54 (4) ◽  
pp. 336-342
Author(s):  
Philipp Körner ◽  
Jana A Schleich ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt

<b><i>Objectives:</i></b> This in vitro study aimed to evaluate and compare the effect of two different bioactive glasses, a hydroxyapatite-containing, fluoride-free toothpaste (HTP) and a fluoride toothpaste (FTP) on the remineralization behavior of initial caries lesions. <b><i>Materials and Methods:</i></b> A total of 100 bovine enamel samples were randomly allocated to five groups of 20 samples each: NC = negative control group (artificial saliva); HTP = HTP group (Karex); FTP = FTP group (Elmex caries protection, 1,400 ppm); FTP + BG<sub>nano</sub> = FTP followed by Actimins bioactive glass; FTP + BG<sub>amorph</sub> = FTP followed by Schott bioactive glass. Radiographic documentation (advanced transversal microradiography; aTMR) was applied before and after all samples were exposed to a demineralizing gel for 10 days. Over a period of 28 days, samples were covered twice a day (every 12 h) with a toothpaste slurry of the respective test group or with artificial saliva in NC for 60 s and brushed with 15 brushing strokes. Samples in FTP + BG<sub>nano</sub> and FTP + BG<sub>amorph</sub> were additionally treated with the respective bioactive glass slurry for 30 s after brushing with the FTP. In the meantime, all samples were stored in artificial saliva. After 28 days, the structure of all samples was assessed again using aTMR and compared to the values measured after demineralization. The statistical evaluation of the integrated mineral loss was performed using Kruskal-Wallis test followed by a post hoc Conover test. <b><i>Results:</i></b> The FTP revealed the significantly highest increase of mineral content while the HTP showed the significantly lowest remineralization. Compared to artificial saliva, the use of the HTP or the combined application of FTP followed by bioactive glasses (FTP + BG<sub>nano</sub> and FTP + BG<sub>amorph</sub>) showed no significant remineralization. <b><i>Conclusion:</i></b> Under remineralizing in vitro conditions, brushing with 1,400 ppm FTP induced significantly more remineralization compared to storage in artificial saliva. The additional administration of both bioactive glasses as well as the substitutional brushing with an HTP resulted in significantly less remineralization compared to brushing with 1,400 ppm FTP.


2018 ◽  
Vol 4 (1) ◽  
pp. 15
Author(s):  
Afiatul Mukarromah ◽  
Irfan Dwiandhono ◽  
Dian Noviyanti Agus Imam

Demineralization is a process of partial or full tooth mineral loss which caused by acidic environment, for example the side effect of extracoronal bleaching treatment. Demineralization increases enamel surface roughness which leads plaque accumulation. Whey extract and calcium phosphopeptide-amorphous calcium phophate (CPP-ACP) contain calcium and phosphate that can stop the demineralization through remineralization process. This study aimed to determine the differences of enamel surface roughness after whey extract and CPP-ACP application post- extracoronal bleaching. Experimental laboratory with pre- and post-test control group design was performed on 24 first maxillary premolars which devided into 3 groups. On group I, specimens were immersed in artificial saliva. Specimens in group II were immersed in whey extract meanwhile specimens were immersed in CPP-ACP on group III. Whey extract and CPP-ACP immersions were conducted 10 minutes every 12 hours for 15 days. The enamel surface roughness test was performed twice, after extracoronal bleaching treatment and after 15 days remineralization agent application. This study result indicated significant differences between group I and group II and between group I and group III (p<0.05), but there was no significant difference between group II and group III (p>0.05). This study showed whey extract decreased more enamel surface roughness than CPP-ACP but the difference was not significant statistically. So, whey extract and CPP-ACP showed similar remineralization potential.


2012 ◽  
Vol 1465 ◽  
Author(s):  
Xin Liu ◽  
Mohammed N. Rahaman

ABSTRACTBioactive glass is an attractive scaffold material for use in filling bone defects because of its widely recognized ability to support the growth of bone cells and to bond firmly with hard and soft tissue. Use of bioactive glasses in the form of porous three-dimensional scaffolds for bone repair applications has been receiving considerable interest in recent years. However, bioactive glass scaffolds have been limited to the repair of low-load bone defects because of their low strength. In the present work, porous and strong bioactive glass scaffolds with an oriented microstructure were prepared by unidirectional freezing of camphene-based suspensions, and evaluated for their ability to regenerate bone in a non-healing rat calvarial defect model. Scaffolds of 13-93 glass (53SiO2, 6Na2O, 12K2O, 5MgO, 20CaO, 4P2O5; wt%) with a porosity of 50% and columnar pores of diameter 50–150 μm showed a compressive strength of 47 ± 5 MPa and an elastic modulus of 11 ± 3 GPa. Total bone regeneration in the oriented scaffolds, 18% after implantation for 12 weeks to 24% after 24 weeks, was not significantly different from that in 13-93 scaffolds with a microstructure similar to that of dry human trabecular bone (control group). The results indicated that these oriented bioactive glass (13-93) scaffolds could potentially be used in the regeneration of loaded bone.


2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Bo Zhang ◽  
Sibei Huo ◽  
Shiyu Liu ◽  
Ling Zou ◽  
Lei Cheng ◽  
...  

Tooth bleaching is becoming increasingly popular among patients with tooth staining, but the safety of bleaching agents on tooth structure has been questioned. Primarily thriving on the biofilm formation on enamel surface, Streptococcus mutans has been recognized as a major cariogenic bacterial species. The present study is aimed at investigating how cold-light bleaching would change enamel roughness and adhesion of Streptococcus mutans. Human premolars were divided into 72 enamel slices and allocated into 3 groups: (1) control, (2) cold-light bleaching with 35% hydrogen peroxide (Beyond™), and (3) 35% hydrogen peroxide (Beyond™) alone. Biofilms of Streptococcus mutans were cultivated on enamel slices in 5% CO2 ( v / v ) at 37°C for 1 day or 3 days. Enamel surfaces and biofilms were observed using scanning electron microscope (SEM). Atomic force microscopy (AFM) was applied to quantify the roughness of enamel surface, and the amounts of biofilms were measured by optical density of scattered biofilm and confocal laser scanning microscopy (CLSM). Cold-light bleaching significantly increased ( p < 0.05 ) surface roughness of enamel compared to controls, but significantly inhibited ( p < 0.05 ) adhesion of Streptococcus mutans on enamel in the bacterial cultures of both 1 day and 3 days. In conclusion, cold-light bleaching could roughen enamel surface but inhibit Streptococcus mutans adhesion at the preliminary stage after the bleaching treatment.


2020 ◽  
Vol 92 (2) ◽  
pp. 20701
Author(s):  
Bo Li ◽  
Xiaofeng Li ◽  
Zhifeng Zhu ◽  
Qiang Gao

Laser-induced breakdown spectroscopy (LIBS) is a powerful technique for quantitative diagnostics of gases. The spatial resolution of LIBS, however, is limited by the volume of plasma. Here femtosecond-nanosecond dual-pulsed LIBS was demonstrated. Using this method, the breakdown threshold was reduced by 80%, and decay of continuous radiation was shortened. In addition, the volume of the plasma was shrunk by 85% and hence, the spatial resolution of LIBS was significantly improved.


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