Plasma Total Antioxidant Capacity in Hemodialyzed Patients and Its Relationships to other Biomarkers of Oxidative Stress and Lipid Peroxidation

2002 ◽  
Vol 40 (2) ◽  
Author(s):  
GianMario Gerardi ◽  
Mario Usberti ◽  
Giuliana Martini ◽  
Alberto Albertini ◽  
Lidia Sugherini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Total Antioxidant ◽  
Biomarkers Of Oxidative Stress

scholarly journals Total Antioxidant Capacity and Lipid Peroxidation Status in Cervical Cancer Patients Compared with Women Without Cervical Cancer in Bangladesh

2021 ◽  
Vol 19 (4) ◽  
Author(s):  
Taslima Nigar ◽  
Annekathryn Goodman ◽  
Shahana Pervin
Keyword(s):  
Oxidative Stress ◽  
Cervical Cancer ◽  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Cancer Patients ◽  
Total Antioxidant Capacity ◽  
Stress Index ◽  
Oxidative Stress Index ◽  
Total Antioxidant

Abstract Purpose Over the past several decades, research has suggested reactive oxygen species act as cofactors for cervical cancer development. The aim of this study is to evaluate the antioxidant and lipid peroxidation status in cervical cancer patients in Bangladesh. Methods From December 2017 to 2018, a cross-sectional observational study was conducted on 50 cervical cancer patients and 50 controls. Plasma levels of lipid peroxidation and total antioxidant capacity were measured. The Student’s t test was used for statistical analysis. P values less than 0.05 were taken as a level of significance. Results There was a significant reduction in total antioxidant levels in patients with cervical cancer, 972.77 ± 244.22 SD µmol equivalent to ascorbic acid/L, compared to normal controls, 1720.13 ± 150.81 SD µmol equivalent to ascorbic acid/L (P < 0.001). Levels of lipid peroxidation were found to be significantly higher in cervical cancer, 7.49 ± 2.13 SD µmol/L, than in women without cervical cancer, 3.28 ± 0.58 SD µmol/L (P < 0.001). The cervical cancer patients had significantly higher levels of oxidative stress index (0.83 ± 0.31) in comparison to controls (0.19 ± 0.04) (P < 0.001). Conclusion There was an increased oxidative stress index due to imbalance between lipid peroxidation generation and total antioxidant capacity in cervical cancer patients. Further studies are needed to explore the role of oxidative stress as a cofactor for cervical carcinogenesis.

scholarly journals Evaluation of Salivary and Serum Total Antioxidant Capacity and Lipid Peroxidation in Postmenopausal Women

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Fatemeh Zovari ◽  
Hadi Parsian ◽  
Ali Bijani ◽  
Ameneh Moslemnezhad ◽  
Atena Shirzad
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Postmenopausal Women ◽  
Total Antioxidant Capacity ◽  
Premenopausal Women ◽  
Control Group ◽  
Antioxidant Power ◽  
Whole Saliva ◽  
Total Antioxidant

Objective. In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women. Methods. In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann–Whitney test and Spearman’s correlation test were used. P < 0.05 was considered statistically significant. Results. The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( P < 0 < 001 and P < 0.01 , respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( P = 0.64 and P = 0.08 , respectively). Conclusion. In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.

Oxidative stress in operating room personnel: occupational exposure to anesthetic gases

2005 ◽  
Vol 24 (11) ◽  
pp. 597-601 ◽  
Author(s):  
A Akbar Malekirad ◽  
A Ranjbar ◽  
K Rahzani ◽  
M Kadkhodaee ◽  
A Rezaie ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Operating Room ◽  
Total Antioxidant Capacity ◽  
Plasma Lipid ◽  
The Body ◽  
Operating Room Personnel ◽  
Total Antioxidant ◽  
Anesthetic Gases

Health professionals exposed to anesthetic gases are at higher risk of reproductive, neurological, hematological, immunological, hepatic and renal system diseases. We investigated if oxidative stress induced by chronic exposure to anesthetic gases has any association with this matter. Plasma lipid peroxidation, total antioxidant capacity and total thiol molecule levels were measured in 66 operating room staff in comparison with 66 controls. The exposed group had a significantly higher level of lipid peroxidation with decreased thiol groups compared to control subjects. Total antioxidant capacity of the body was no different among exposed and not exposed subjects. Increased lipid peroxidation in the blood of exposed subjects warns that oxygen free radicals have increased in the body and thus might attack cells, which, in the longterm, results in multi-organ damage. The remaining blood total antioxidant capacity at normal values is promising and means that other non-thiol antioxidants, such as uric acid, transferrin, ceruloplasmin, albumin, and vitamin antioxidants, such as a-tocopherol and ascorbic acid, have been stimulated to maintain the total anti-oxidant power of the body at normal state.

scholarly journals LIFE HABITS AND LIPID PEROXIDATION OF WOMEN OVER 40 YEARS OLD

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S148-S148
Author(s):  
Renata F Oliveira ◽  
Maria Mota ◽  
Jorge Soares ◽  
Zirlene Santos ◽  
Bianca Rosa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Catalase Activity ◽  
Total Antioxidant Capacity ◽  
Multiple Linear Regression Model ◽  
Activity Level ◽  
Leg Strength ◽  
Total Antioxidant

Abstract Oxidative stress is involved in degenerative processes, aging, and diseases. Lifestyle can be change oxidative stress. One of reactive oxygen species targets is polyunsaturated fatty acid, an important cellular membrane component. The aim of this study is to analyse the contribute of lifestyle in lipid peroxidation in over fourty years old women. Were included 60 women whith age ranged between 41 and 82 years old (53.3 ± 9.1 years). Lifestyle were explored doing a food frequency questionnaire, Perceptive Stress Scale (PSS) and International Physical Activity Questionnaire (IPAQ). The functional capacity was determinate by 6-min walking test (cardiorespiratory capacity) and Squat-jump (leg strength and power). Parameters of oxidative stress were determinate from plasma during fasting, lipid peroxidation was evaluated by TBARs, and antioxidant capacity was evaluated by catalase activity and ABTS. Spearman correlation and Multiple Linear Regression model, through Stepwise method, considering TBARs as dependent variable, and age, weight, body mass index, waist circumference, stress perception, physical activity level, total antioxidant capacity, catalase activity, cardiorespiratory capacity, leg strength and power, daily caloric intake, daily fruit, vegetables, coffee/tea, vitamin E and alcohol intake, as independent variable, was performed. Negative correlations were obtained between TBARs and cardiorespiratory capacity (r= -0.35; p=0.026) and between TBARS and ABTS (r= -0.33; p=0.038). Total antioxidant capacity was the model’s first variable (F= 5.50; p = 0.013), explaining 15.3% of TBARS, then cardiorespiratory capacity (F= 5.50; p = 0.047), explaining 10.5% of TBARs The results revealed total antioxidant capacity and cardiorespiratory capacity as predictors to lipid peroxidation damage.

Protection by sildenafil and theophylline of lead acetate-induced oxidative stress in rat submandibular gland and saliva

2003 ◽  
Vol 22 (11) ◽  
pp. 587-592 ◽  
Author(s):  
Mohammad Abdollahi ◽  
Fatemeh Fooladian ◽  
Bita Emami ◽  
Khatereh Zafari ◽  
Arezou Bahreini-Moghadam
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Lead Acetate ◽  
Lead Toxicity ◽  
Thiol Groups ◽  
Total Antioxidant ◽  
Camp And Cgmp

The role of oxidative stress in lead toxicity has been proposed in many organs, however, no study has been performed in the salivary glands, which are important parts of the gastrointestinal tract with a high implication in health of the whole body. Recently, it has been proposed that increasing the levels of cGMP and cAMP in the cells may protect from the neurotoxicity of lead. The objective of this study was to determine the ability of lead acetate to produce oxidative stress in rat submandibular as the main salivary gland of the body and to study the role of pretreatment by specific phosphodiesterase inhibitors in the prevention of oxidative stress. Lead acetate (100 mg/kg), alone or in combination with theophylline (25 mg/kg) and sildenafil (5 mg/kg), was administered intraperitoneally to rats. After 2 hours and under general anaesthesia, the submandibular gland ducts were cannulated intraorally using microcannula, and pure saliva was collected for 30 min using pilocarpine (8 mg/kg) as a secretagogue. The submandibular glands were then isolated free under surgery. Oxidative stress in the gland and pure saliva were evaluated measuring lipid peroxidation (thiobarbituric acid reactive substances assay), total thiol groups content and total antioxidant capacity (the ferric reducing ability assay). Results showed significant oxidative stress in the gland and secretions as indicated by increased lipid peroxidation, decreased total antioxidant capacity and thiol group levels. The use of cAMP and cGMP phosodiesterase inhibitors, theophylline and sildenafil, prevented leadinduced increased lipid peroxidation and also protected from decreased thiol groups content and total antioxidant power of the gland and secretions. The same trend of effects was observed in gland and saliva. It is concluded that lead toxicity is mediated through oxidative stress in salivary glands, while increasing intracellular cAMP and cGMP levels may prevent lead-induced oxidative stress.

scholarly journals P378 Oxidative status and antioxidant capacity in children and adults with IBD: preliminary results of the OxIBDiet trial

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S392-S393
Author(s):  
S Gatti ◽  
L Cianfruglia ◽  
T Galeazzi ◽  
A Palpacelli ◽  
S Quattrini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Antioxidant Capacity ◽  
Protein Oxidation ◽  
Total Antioxidant Capacity ◽  
Oxidative Status ◽  
Healthy Controls ◽  
Preliminary Results ◽  
Total Antioxidant

Abstract Background Redox imbalance is involved in several aspects of inflammatory bowel disease (IBD). The OxIBDiet (NCT04513015) is a multicentre, 2-phases project involving IBD children and adults with the following aims: 1. To evaluate oxidative status of IBD subjects. 2. To estimate the effects of an antioxidant diet in IBD patients. Preliminary results are shown in this abstract. Methods The total antioxidant capacity, lipid peroxidation and the degree of protein oxidation were measured respectively through the ferric reducing ability of plasma (FRAP, µmol/equivalent FeSO4), serum levels of the thiobarbituric acid reactive substances (TBARs, µmol MDA) and advanced Oxidation Protein Products (AOPP, µmol/g protein). Reactive oxygen species (ROS, Arbitrary Units) and activities (nmol/min/mg of protein) of the main antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S-Transferase (GST) and catalases (CAT) were evaluated in PMNs cells. Oxidative stress markers were correlated with demographic variables and clinical parameters. Results Fifteen adults (mean age: 36.2 ±11.4 years, 47% female, 67% in remission) and thirty five children (mean age: 14.13 ±2.2 years, 60% female, 65% in remission) with IBD have been enrolled so far. FRAP was significantly reduced in IBD children compared to healthy controls (median values: 212.1 vs. 248.3, p=0.0001) and to IBD adults (median value: 281, p&lt;0.001), while no difference was observed between adults with IBD and the control group (p= 0.1). ROS levels did not differ in IBD children compared to adults and controls. The activity of GPX and CAT enzymes was increased in children with IBD in comparison to controls (p:0.02 and 0.001, respectively) while the activity of the other enzymes (GST, GR and SOD) and levels of lipid peroxidation and protein oxidation was not different between the 2 groups. Overall in the IBD group (children plus adults) FRAP was positively correlated with age (r=0.40, p=0.006), male gender (r=0.33, p=0.03) and use of biologics (r=0.47, p=0.001) and inversely correlated to disease activity based on clinical scores (r= -0.38, p=0.009). No correlation was found between FRAP and serum C-reactive protein or calprotectin levels. Conclusion The total antioxidant capacity (FRAP) is significantly impaired in IBD children respect to IBD adults and to healthy controls, thus suggesting an early involvement of oxidative stress in IBD pathogenesis. Moreover, the activity of the main antioxidant enzymes (GPx and CAT) in IBD children is increased, as a possible compensation for redox imbalance. Final results will clarify the involvement of antioxidant cascade in IBD pathogenesis and in therapeutic approach.

Silibinin and Nano-Silibinin in Cuprizone Model of Multiple Sclerosis: Behavioral and Biochemical Investigation

2021 ◽  
Vol 17 ◽  
Author(s):  
Yasin Ahmadi ◽  
Mohammad Mehdi Mahboobian ◽  
Mojdeh Mohammadi
Keyword(s):  
Oxidative Stress ◽  
Multiple Sclerosis ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Capacity ◽  
Open Field ◽  
Total Antioxidant Capacity ◽  
Tail Flick ◽  
Total Antioxidant ◽  
Antioxidant Parameters

Objectives: Multiple sclerosis (MS) is a long-lasting demyelinating inflammatory disease of the central nervous system (CNS). It has been shown that brain tissue in MS is exposed to oxidative stress during the disease period. Silymarin, a plant-derived flavonoid, can be extracted from Silybum marianum. The current experiment aimed to explore the effects of silibinin and especially nano-silibinin on neurobehavioral activity and biochemical antioxidant parameters in the cuprizone model of demyelination in mice for the first time. Methods: Demyelination was induced in mice by oral consumption of cuprizone 0.4%w/w for one week and then 0.2%w/w for four weeks. Treatment was performed with silibinin or nano-silibinin (70mg/kg body weight) for four weeks at the same time with cuprizone 0.2%w/w. After neurobehavioral tests (rotarod, tail flick, and open field), biochemical antioxidant parameters (glutathione level, superoxide dismutase activity, lipid peroxidation products, and total antioxidant capacity) were evaluated. Results: In this experiment, behavioral tests (rotarod and open field) displayed improvement in movement dysfunction using silibinin or nano-silibinin. Furthermore, silibinin and more efficiently nano-silibinin increased antioxidant parameters, such as superoxide dismutase (SOD) and glutathione (GSH) and total antioxidant capacity (TAC), and decreased lipid peroxidation. Conclusion: These data suggest that silibinin and nano-silibinin can improve movements in the cuprizone model of demyelination. Moreover, they may prevent cuprizone-induced oxidative stress. In conclusion, silibinin and more effectively, nano-silibinin, may exhibit therapeutic features in MS disease.

scholarly journals Evaluation of Oxidative Stress Markers and Hormonal Profiles in Women Diagnosed with Infertility in Port Harcourt

2019 ◽  
pp. 1-11
Author(s):  
Olofinshawo, Olanrewaju Lawrence ◽  
Bartimaeus, Ebirien-Agana Samuel ◽  
Davies, Tamuno-Emine Gabriel
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Infertile Women ◽  
Stress Markers ◽  
Standard Procedures ◽  
Port Harcourt ◽  
Total Antioxidant ◽  
Peroxidation Index

Aim: The study evaluates the contribution of oxidative stress and fertility hormones to female infertility in Port Harcourt. Methodology: A total of 140 women aged 15 – 49 years consisting 70 apparently healthy infertile women who are attending diagnostic fertility clinics in Port Harcourt as test subjects and 70 age-matched healthy fertile women as control were recruited. Subjects were recruited using structured questionnaires after given their informed consent. The levels of Malondialdehyde, total antioxidant capacity, lipid peroxidation index, follicle stimulating hormone, luteinizing hormone, prolactin, progesterone and estrogen of infertile and the fertile (control) subjects were determined by standard procedures. Results: There was statistically significant increase in lipid peroxidation index in the test subjects than in the fertile group (p<0.05). Total antioxidant capacity showed a statistically reduced value in the test subjects than in the control at p<0.05. Lipid peroxidation index was significantly increased  in test subjects exposed to oxidant agents like alcohol, infections and ulcer than their counterparts who were not exposed to any of the agents (p<0.05). Also significantly elevated lipid peroxidation was observed in test subjects with normal hormone levels compared to those with hormone imbalance (p<0.05). Conclusion: The outcome of this study suggests that the infertility being experienced by some of the infertile women in Port Harcourt are due not only to endocrine dysfunction, but some order conditions that induce oxidative stress. Thus investigation of oxidative parameters is highly suggested as an adjunct for effective management of unexplained infertility in women. Further studies on estimation of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GHR) are recommended to validate reliability.

Sign in / Sign up

Export Citation Format

Share Document