The effect of nitrite food preservatives added to se’i meat on the expression of wild-type p53 protein

AbstractThis research was conducted using beef extracted from Kupang (se’i meat), Indonesia. Se’i meat is a locally found food where the preferred mode of preparation is smoking the beef with the preservation using nitrites. Nitrite can cause health-related problems such as cancer. This research was carried out using a true experimental method with a complete randomized design with the aim of analyzing the effect of meat administration on the expression of wild-type p53 protein in colon cells of Balb/c mice as an indicator of carcinogenesis. The measurement of p53 is to observe the increase in the-over-capacity of p53 expression in the colon cell as a result of decrease in wild-type protein p53. This research provides scientific information about the effect of giving se’i meat on the expression of wild-type p53 in cells of Balb/c mice as an indicator of carcinogenesis. A total of 36 male mice of Balb/c strain weighing 23.8 g were divided into four groups classified as samples (P1, P2 and P3) and control (K), which were taken from modern and home industries in the city of Kupang. The results showed that consumption of nitrite-preserved beef se’i (traditional smoked meat) increased the p53 protein expression in colon cells of Balb/c strain male mice, and the least significant difference test also showed that there were differences in wild-type p53 protein expression among the four groups: P1 (mice that have been given the standard food, drinking water and se’i meat that contains no nitrite) has an average of 142 expressions, which is higher than that of P3 (mice that have been given the standard food, drinking water and se’i meat containing nitrite which come from the home industry) which has an average of 106.55 expressions and is higher than that of K (mice that have been given the standard food and drinking water) which has the total average of expression of about 78.11 expressions. The benefit of this research is to gain the scientific information about the effect of giving smoked meat on the expression of wild-type p53 in colon cells of Balb/c mice as a carcinogenic indicator.

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p53 protein expression in non-Hodgkin's lymphoma. Comparative study with the wild type p53 induced proteins mdm2 and p21/waf1

Molecular Pathology ◽

10.1136/mp.49.5.m278 ◽

1996 ◽

Vol 49 (5) ◽

pp. M278-M282 ◽

Cited By ~ 3

Author(s):

M Tzardi ◽

C. Kouvidou ◽

I Panayiotides ◽

K Stefanaki ◽

D Rontogianni ◽

...

Keyword(s):

Protein Expression ◽

Comparative Study ◽

P53 Protein ◽

Hodgkin's Lymphoma ◽

Wild Type ◽

P53 Protein Expression ◽

Non Hodgkin's Lymphoma ◽

Wild Type P53 ◽

P21 Waf1 ◽

Induced Proteins

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Effect of the specific P53 stabilizer CBS9106 on multiple myeloma (MM)

Journal of Clinical Oncology ◽

10.1200/jco.2009.27.15_suppl.8601 ◽

2009 ◽

Vol 27 (15_suppl) ◽

pp. 8601-8601

Author(s):

H. Ikeda ◽

T. Hideshima ◽

G. Perrone ◽

Y. Okawa ◽

N. Raje ◽

...

Keyword(s):

Cell Cycle ◽

Multiple Myeloma ◽

Protein Expression ◽

Tumor Cells ◽

Cell Lines ◽

Primary Tumor ◽

P53 Protein ◽

Wild Type ◽

Cycle Arrest ◽

P53 Protein Expression

8601 Background: The mutations of P53 tumor suppressor protein are associated with progressive in Multiple Myeloma (MM), conversely, stabilization of P53 leads to cell cycle arrest and apoptosis. In this study, we examined p53 protein expression and demonstrated the effect of P53 stabilization using a novel specific P53 stabilizer CBS9106 in MM. Method: We examined P53 protein expression using Immunoblot analysis, as well as the growth inhibitory effect of CBS9106 in MM cell lines and primary tumor cells from MM patients. We also defined whether CBS9106 can overcome the growth promoting effect of exogenous cytokines and bone marrow stroma cells (BMSCs) using [3H]-thymidine uptake assay. Results: Expression of P53 protein was observed in 3/3 primary tumor cells from MM patients and 6/6 MM cell lines. CBS9106 at low nM levels triggered cytotoxicity against p53 wild type MM cell lines and primary tumor cells from MM patients, associated with phosphorylation of P53 (serine15 and 20). In contrast, CBS9106 did not affect the survival of normal peripheral blood mononuclear cells from healthy volunteers at concentrations as high as 10 μM. This agent also induced G1 cell cycle arrest, followed by apoptosis associated with cleavage of caspase-3, -8, -9 and PARP. Neither growth stimulating cytokines (IL-6 and IGF-1) nor BMSCs protected against apoptotic effect of CBS9106. Moreover, we demonstrate that combination of CBS9106 with MDM2 inhibitor Nutrin3 or proteasome inhibitor bortezomib induces synergistic anti-MM activity in both P53 wild type MM cell lines and primary tumor cells from MM patients. Conclusions: Stabilizing P53 by CBS9106 represents a novel promising p53-based therapy in MM. These results provide the preclinical framework supporting evaluation of CBS9106 in clinical trials to improve patient outcome in MM. No significant financial relationships to disclose.

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Investigations of oral uracil and tegafur(UFT)-based adjuvant chemotherapy in patients with colon cancer

Journal of Clinical Oncology ◽

10.1200/jco.2006.24.18_suppl.13535 ◽

2006 ◽

Vol 24 (18_suppl) ◽

pp. 13535-13535

Author(s):

S. Noshima

Keyword(s):

Colon Cancer ◽

Treatment Group ◽

Adjuvant Chemotherapy ◽

Protein Expression ◽

Surgical Resection ◽

P53 Protein ◽

P53 Protein Expression ◽

Preoperative Administration ◽

Significant Difference ◽

Mib 1

13535 Background: Surgical resection plays a major role in the treatment of colon cancers. However, surgical resection alone has limitation in its use and it is necessary to administer adjuvant therapy and it would be important to define clinical significance of adjuvant chemotherapy. Methods: A total of 95 patients diagnosed with colon carcinoma were randomly assigned to one of two groups, i.e., treatment-group or non-treatment group. UFT (400 mg/day) was administered daily to 40 patients (treatment-group), one week before surgery. 55 patients (non-treatment group) received no medication before undergoing surgery. In the present study, we have investigated how adjuvant chemotherapy inhibited proliferation of colon cancer cells in vivo through monitoring cell proliferative activities (using MIB-1 antibody), expression of abnormal p53 protein and apoptosis-assays. Two groups were subjected to analyses of these study parameters. Results: Effects of preoperative administration of UFT on cellular proliferative activities: MIB-1-positive rates were 21.21 ± 2.89 (%) for treatment group and 39.02 ± 2.27 (%) for non-treatment group, and there was significant difference in MIB-1-positive-rates between the two groups (P<0.0001). Effects of preoperative administration of UFT on mutated p53 protein expression: The incidence of mutated p53 protein was 20.27 ± 4.28 (%) for treatment group and 49.06 ± 4.46 (%) for non-treatment group, and there was significant difference in the incidence of mutated p53 protein expression between the two groups (P<0.0001). Effects of preoperative administration of UFT on apoptosis: Apo.I. was 1.24 ± 0.20 (%) for treatment group and 0.49 ± 0.06 (%) for non-treatment group, and there was significant difference in Apo.I. between the two groups (P<0.001). Conclusions: Taken together, these findings indicate that UFT-based adjuvant chemotherapy is potentially useful for treatment of colon cancer patients undergoing surgical resection. No significant financial relationships to disclose.

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Regulation of p53 protein expression in human breast cancer cell lines

Journal of Cell Science ◽

10.1242/jcs.105.3.607 ◽

1993 ◽

Vol 105 (3) ◽

pp. 607-612 ◽

Cited By ~ 1

Author(s):

B. Vojtesek ◽

D.P. Lane

Keyword(s):

Protein Expression ◽

Cell Lines ◽

P53 Protein ◽

Critical Role ◽

P53 Gene ◽

Breast Cancer Cell Lines ◽

Breast Cancers ◽

Human Breast ◽

Wild Type ◽

P53 Protein Expression

Mutation of the p53 gene is a common occurrence in human breast cancers but is by no means universal. However, even in tumours where the gene is not mutated altered levels of p53 protein are often detected. This is also observed in cell lines derived from human breast cancers. By transfecting such cell lines containing either wild type or mutant p53 genes with a temperature-sensitive mutant mouse p53 gene we have established that the cellular environment plays a critical role in the regulation of p53 protein expression. The results suggest that tumours that aberrantly express wild-type p53 may have lost the normal growth regulatory response to the protein and thus be functionally similar to those expressing the mutant protein.

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