Avocado/soy unsaponifiables can redress the balance between serum antioxidant and oxidant levels in patients with osteoarthritis: a double-blind, randomized, placebo-controlled, cross-over study

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Zohreh Jangravi ◽  
Saeed Basereh ◽  
Ali Zaree Mahmoudabadi ◽  
Mehdi Saberi ◽  
Gholam Hossein Alishiri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Reduced Glutathione ◽  
Mixed Effects ◽  
The Other ◽  
Inflammatory Disorder ◽  
Double Blind ◽  
Total Antioxidant ◽  
And Oxidative Stress

Abstract Objectives Osteoarthritis (OA) is an inflammatory disorder of the joint characterized by pain and stiffness. Oxidative stress plays an important role in pathogenesis of OA. We aimed to evaluate the effects of avocado/soy unsaponifiables (ASU) compound on serum antioxidant and oxidative stress in patients with Osteoarthritis. Methods A double-blind, randomized, placebo-controlled, cross-over trial was performed. Fourty patients with osteoarthritis were randomized to two different sequences: 1) DP: received ASU for three months followed by three months placebo, 2) PD: received placebo for the first three months followed by three months ASU. The oxidant statue was evaluated by measurement of serum malonldialdehyde (MDA). The total antioxidant capacity (TAC), reduced glutathione (GSH) and antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) were also assessed. Results Mean serum of MDA level as a marker of oxidative stress significantly decreased in all patients after three months treatment with ASU (4.46 ± 0.11 nmol/L) compare with baseline and placebo levels (5 ± 0.15 and 5.82 ± 0.12 nmol/L respectively) (p<0.05). On the other hand, ASU resulted in positive changes in serum antioxidant levels (p<0.05). Mixed-effects model of variance analyses showed that ASU effect is regardless of the order of receiving medication (p>0.05). Conclusions These data showed that Avocado/Soy Unsaponifiable can be an effective supplement in treatment of osteoarthritis through the control of the balance between antioxidant and oxidant molecular markers.

scholarly journals Effects of Safranal on Tissue Oxidative Stress in Sub-Chronic Thinner-Addicted Rats

2020 ◽  
Vol 71 (1) ◽  
pp. 1997
Author(s):  
M. DÜZ ◽  
A. F. FIDAN
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Protective Effect ◽  
Total Antioxidant Capacity ◽  
Reduced Glutathione ◽  
Control Group ◽  
Albino Rats ◽  
Total Antioxidant ◽  
Wistar Albino Rats ◽  
And Oxidative Stress

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance and oxidative stress on certain tissues and the possible protective effect of safranal against thinner toxication in rats. Adult male Wistar albino rats were randomly divided into four groups of 10 animals each as follows: control (C), safranal (S), thinner (T) and thinner+safranal (T+S). The control group received 1cc saline by gastric gavage. Safranal was administered to S and T+S groups by using gastric gavage at a dose of 100 mg/kg/day and volume of 0.1 mL/kg/day. Thinner inhalation was applied to T and T+S groups in a container with NaOH tablets twice a day. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NOx) metabolites, total antioxidant capacity (TAS) and total oxidant capacity (TOS) were determined in liver, lung, brain, kidney and testis tissues of the rats. In the T+S group, it was observed that the MDA levels significantly decreased in all tissues, except the kidney, in comparison to the thinner inhalation group (p = 0.000). When the NOx levels of the T+S group were compared with the levels of the T group, it was concluded that there existed a statistically significant decrease in the NOx levels in alltissues (p = 0.000). In T+S group, it was observed that safranal either eliminated or mitigated oxidative stress that developed in tissues through decreasing MDA and TOS levels and increasing GSH and TAS levels and caused significant decreases in NOX levels in all tissues. As a result, it was determined that safranal, although not uniform for all tissue types, had a protective potential against the damaging effects of oxidative stress caused by sub-chronic thinner inhalation.

scholarly journals Effect of silymarin on sodium fluoride-induced toxicity and oxidative stress in rat cardiac tissues

2012 ◽  
Vol 84 (4) ◽  
pp. 1121-1126 ◽  
Author(s):  
Seyed M. Nabavi ◽  
Seyed F. Nabavi ◽  
Akbar H. Moghaddam ◽  
William N. Setzer ◽  
Morteza Mirzaei
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Sodium Fluoride ◽  
Biochemical Parameters ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Glutathione Level ◽  
Thiobarbituric Acid Reactive Substances ◽  
Cardiac Tissues ◽  
And Oxidative Stress

This study aim to evaluate the protective effect of silymarin on sodium fluoride-induced oxidative stress in rat cardiac tissues. Animals were pretreated with silymarin at 20 and 10 mg/kg prior to sodium fluoride consumption (600 ppm through drinking water). Vitamin C at 10 mg/kg was used as standard antioxidant. There was a significant increase in thiobarbituric acid reactive substances level (59.36 ± 2.19 nmol MDA eq/g tissue) along with a decrease in antioxidant enzymes activity (64.27 ± 1.98 U/g tissue for superoxide dismutase activity and 29.17 ± 1.01 µmol/min/mg protein for catalase activity) and reduced glutathione level (3.8 ± 0.15 µg/mg protein) in the tissues homogenates of the sodium fluoride-intoxicated rats. Silymarin administration to animals before sodium fluoride consumption modified the levels of biochemical parameters.

scholarly journals Gut microbiota shift of spangled emperor under pollution stress

2021 ◽  
Author(s):  
Othman Baothman ◽  
Salem A-Zahrani ◽  
Hasan Al-Talhi
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Gut Microbiota ◽  
Positive Relationship ◽  
The Other ◽  
Significant Elevation ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Bacterial Richness

Hepatic antioxidant enzymes as oxidative stress biomarkers were investigated and correlated with the identified dominant gut microbial phyla. The results showed that while the antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT) levels were reduced in the polluted PO site, significant elevation (*P ≥ 0.05) was observed at the clean reference CR site indicating negative correlation to pollution stress. On the other hand, among five significant bacterial genera, Lactobacillus and Vagococcus showed a positive relationship to the oxidative pollution stress between PO and CR sites. Diversity and bacterial richness had been observed in the PO site compared to the CR site. As a result, 429,346 sequences were obtained from the pooling of 20 samples identified into 10 phyla and 79 genera in which Firmicutes was dominant in both PO and CR sites. The number of common OTUs was 221 for both CR and PO samples. The results revealed that under the stressed environmental state, the homo-lactic Vagococcus genus is dominant over the hetero-lactic Lactobacillus, which uses less energy in the derived process.

Oxidative stress and vitamin D receptor BsmI gene polymorphism in Egyptian children with systemic lupus erythematosus: a single center study

Lupus ◽  
2019 ◽  
Vol 28 (6) ◽  
pp. 771-777 ◽  
Author(s):  
Hend Fouad ◽  
Sohier Yahia ◽  
Afaf Elsaid ◽  
Ayman Hammad ◽  
Yahya Wahba ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Lupus Erythematosus ◽  
Odds Ratio ◽  
Glutathione S Transferase ◽  
Bsmi Polymorphism ◽  
Stress Markers ◽  
Egyptian Children ◽  
Total Antioxidant ◽  
And Oxidative Stress

Systemic lupus erythematosus (SLE) is an autoimmune disease with unknown exact etiology. Vitamin D receptor gene ( VDR) and oxidative stress play important roles in the pathogenesis of SLE. Here we investigated the genotypes and allelic frequencies of VDR BsmI polymorphism as well as their relationship with oxidative stress markers in Egyptian SLE children. We conducted a cross-sectional comparative study at Mansoura University Children's Hospital, Egypt from 2014 to 2018 including 100 SLE children and 100 controls. We investigated both groups for VDR BsmI polymorphism using polymerase chain reaction. Oxidative stress was assessed using malondialdehyde, glutathione S-transferase, superoxide dismutase, catalase and total antioxidant capacity. BB genotype frequency was found to be significantly higher in the SLE group ( p = 0.04, odds ratio (95% confidence interval) = 2.5 (1.01–5.9)). However, VDR B allele and b allele showed insignificant differences between SLE patients and controls ( p = 0.36, odds ratio (95% confidence interval) = 1.2 (0.8–1.8)). Lower levels of glutathione S-transferase, superoxide dismutase and total antioxidant capacity were found in the SLE group with statistically significant differences as regards glutathione S-transferase and superoxide dismutase ( p < 0.001). Serum malondialdehyde and catalase levels were significantly higher in the SLE group ( p < 0.001). No significant differences were found between VDR BsmI polymorphism (genotypes and alleles) and oxidative stress markers in the SLE group. In conclusion, BB genotype of VDR BsmI polymorphism is associated with an increased risk of SLE among Egyptian children. Oxidative stress may contribute in pathogenesis of SLE but is not associated with VDR BsmI polymorphism.

scholarly journals Carvacrol attenuates cyclophosphamide-induced oxidative stress in rat kidney

2017 ◽  
Vol 95 (7) ◽  
pp. 844-849 ◽  
Author(s):  
Sibel Gunes ◽  
Adnan Ayhanci ◽  
Varol Sahinturk ◽  
Diler Us Altay ◽  
Ruhi Uyar
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Oxidative Damage ◽  
Tissue Damage ◽  
Total Antioxidant Capacity ◽  
Rat Kidney ◽  
Control Group ◽  
Antioxidative Effect ◽  
Total Antioxidant ◽  
And Oxidative Stress

Cyclophosphamide (CP) is an antineoplastic drug that induces kidney damage via producing oxidative stress. Carvacrol (CAR) has antioxidative effect and we postulated that it can be protective against CP-induced nephrotoxicity. Six groups (n = 7) of rats (control, 100 mg/kg CP, CP+5 mg/kg CAR, CP+10 mg/kg CAR, 5 mg/kg CAR, and 10 mg/kg CAR) were injected intraperitoneally. Serum malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), creatinine (CRE), total antioxidant capacity (TAC), and total oxidant state (TOS) were measured, and oxidative stress indexes (OSI) were calculated. Kidneys were also analyzed histologically. In CP-alone group MDA, CRE, TOS, and OSI levels increased whereas GSH, SOD, CAT, and TAC levels decreased compared with control group. In CP plus CAR groups, MDA, TOS, and OSI levels decreased whereas GSH, SOD, CAT, and TAC levels increased compared with CP-alone group. However, CRE levels were similar in CP-alone and CP+5 CAR group whereas decreased in CP+10 CAR group. CP+10 CAR group was significantly different in all parameters (except TAC) from CP+5 CAR group. Kidney microscopy was showed lower tissue damage in CP plus CAR groups. In conclusion, 10 mg/kg CAR is more effective than 5 mg/kg CAR in prevention of CP-induced oxidative damage on kidney.

scholarly journals Oxidative Status and Acute Phase Reactants in Patients with Environmental Asbestos Exposure and Mesothelioma

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Cengizhan Sezgi ◽  
Mahsuk Taylan ◽  
Hadice Selimoglu Sen ◽  
Osman Evliyaoğlu ◽  
Halide Kaya ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Phase ◽  
Asbestos Exposure ◽  
Oxidative Status ◽  
The Other ◽  
Stress Index ◽  
Control Group ◽  
Acute Phase Reactants ◽  
Total Antioxidant ◽  
And Oxidative Stress

Background and Objectives. The aim of this study was to investigate inflammatory indicators and oxidative status in patients with asbestos exposure with and without mesothelioma and to compare results with data from healthy subjects.Methods. Eighty people with exposure to environmental asbestos and without any disease, 46 mesothelioma patients, and a control group of 50 people without exposure to environmental asbestos were enrolled in this prospective study. Serum total oxidant level (TOL), total antioxidant capacity (TAC), and oxidative stress index (OSI), CRP, transferrin, ceruloplasmin,α-1 antitrypsin, ferritin, and copper levels were measured.Results. Mesothelioma group exhibited higher TOL, OSI,α1-antitrypsin, ferritin and copper levels as compared to the other groups (P<0.001,P=0.007,P<0.0001,P<0.001, andP<0.001, resp.). Transferrin was lower in the mesothelioma group than in the other two groups (P<0.001). The asbestos group had higher TOL, TAC,α1-antitrypsin, and transferrin levels (P<0.001,P<0.001,P<0.001, andP<0.001, resp.), as well as lower OSI and ferritin levels as compared to the control group (P<0.001andP<0.001).Conclusions. We believe that elevated acute phase reactants and oxidative stress markers (TOL and OSI) in the mesothelioma group can be used as predictive markers for the development of asbestos-related malignancy.

scholarly journals Evaluation of Serum Status of Biochemical Indices of Liver Injury and Oxidative Stress in Rats Exposed to Warri River Levels of Pb and Other Identified Metallic Co Pollutants

2018 ◽  
Vol 15 (4) ◽  
pp. 937-947
Author(s):  
E. A. Fadairo ◽  
F.O. Obi
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Liver Injury ◽  
River Water ◽  
Total Protein ◽  
The Other ◽  
Oxidation Products ◽  
Urine Glucose ◽  
River Level ◽  
And Oxidative Stress

This study investigated serum status of biochemical indicators of liver injury and oxidative stress in rats exposed to Warri River level of lead(Pb) alone and in the presence of metallic co-pollutants. A total of 55 albino rats (of Wistar strain) weighing an average of 150.00± 09.00g, divided into 11 groups were used for the study. Groups I and II represented the deionized and Pti borehole water controls, while groups III- XI represented the test rat groups orally treated with water containing laboratory reconstituted Warri River Pb level on one hand and in the presence of laboratory reconstituted identified metallic co-pollutants including Fe, Ca, Cu, Mn, Mg, Zn via water on the other hand. The serum biochemical –hepatotoxic indices investigated were liver/body wt. ratios, body wt. change, lipid per oxidation products, plasma ALT and AST, plasma and liver alkaline phosphatase activities, plasma catalase and superoxide dismutase activities, plasma total and conjugated bilirubin level, plasma and urine glucose concentration, and plasma and urine total protein concentration. Our findings revealed an overall significant (P<0.05) decrease in liver/body wt ratios and body wt change , significant (P<0.05) increase in plasma ALT and AST activities, induced ALP and ACP activities, increase in SOD and catalase activities, increased plasma and urine bilirubin concentrations, decreased plasma and increased urine total protein concentrations, increased Malondialdehyde (MDA) levels, while plasma and urine glucose levels were elevated in the groups of rats exposed to Pb only, Pb + Cu, Pb + Fe and Pb + Zn, Pb + All metallic co-pollutants, and river water relative to their respective controls (deionized water and Pti tap water groups). There was a significant (P<0.05) reversal of the above parameters in the groups of rats exposed to Pb + Ca, Pb + Mn, Pb + Mg. There was also a difference in liver/weight ratio, body wt. change and all the other parameters evaluated in this study, between groups of rats treated with Warri river water relative to the laboratory reconstituted water, although the changes were not significant (P>0.05). Our findings revealed that, the presence of Ca, Mg and Mn in the river water significantly (P<0.05)) reversed the induced activities of ALT, AST, ACP and ALP by Pb and some identified metallic co pollutants like Cu, Fe and Zn. This study also revealed the possibility of significant (P<0.05) decrease in the activities of superoxide dismutase (SOD), catalase , plasma total and direct bilirubin and lipid per oxidation products of rats exposed to Warri River level of Pb in the presence of Ca, Mg and Mn relative to the Pb only group.

Melatonin prevents and ameliorates lead-induced gonadotoxicity through antioxidative and hormonal mechanisms

2018 ◽  
Vol 34 (9) ◽  
pp. 596-608 ◽  
Author(s):  
Luqman Aribidesi Olayaki ◽  
Isiaka Abdullateef Alagbonsi ◽  
Amin Halimat Abdulrahim ◽  
Wale Johnson Adeyemi ◽  
Muftiat Bakare ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Biochemical Markers ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Gonadotropin Releasing Hormone ◽  
Semen Parameters ◽  
Total Antioxidant ◽  
Male Wistar Rats

We investigated the effects of melatonin on sperm parameters and some biochemical markers in lead-exposed male Wistar rats. Lead (50 mg/kg bw/day) and/or melatonin (4 mg/kg or 10 mg/kg bw/day) was administered for 4 weeks, while 2-week lead exposure was preceded by or followed by 2-week treatment with both doses of melatonin in other groups. Lead reduced glutathione, catalase, adjusted testes weight, semen parameters but did not change malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase, and total antioxidant capacity. Though independent of prolactin, lead-induced gonadotoxicity was both centrally and peripherally mediated, as it reduced gonadotropin-releasing hormone and testosterone levels, while gonadotropin levels did not change significantly probably due to negative feedback by elevated estradiol. However, pre-, simultaneous, or posttreatment of lead-exposed rats with melatonin reduced MDA, SOD, and estradiol but dose-dependently increased other parameters. Conclusively, lead causes male gonadotoxicity through oxidative stress and endocrine mechanisms, and these could be dose-dependently prevented and ameliorated by melatonin.

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