Uterine contractile activity and fetal outcome in rats treated with vitamin C during late gestational variable stress exposure

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shakiru A. Salami ◽  
Hussein M. Salahdeen ◽  
Abidemi E. Obafemi ◽  
Babatunde A. Murtala
Keyword(s):  
Vitamin C ◽  
Stress Responses ◽  
Contractile Activity ◽  
Exposed Group ◽  
Magnesium Ions ◽  
Uterine Contractility ◽  
Contractile Responses ◽  
Uterine Contractile ◽  
Vitamin C Supplementation

Abstract Objectives Stress responses vary throughout pregnancy and impact of late gestational variable stress (LGVS) with vitamin C supplementation on uterine contractility is barely explored. This study investigates fetal weight outcome and in-vitro uterine contractile responses to pharmacological agents during LGVS exposure. Methods Twenty four nulliparous pregnant rats were divided into four groups of six. During gestation days 10–19, groups 1 & 2 received normal saline and vitamin C (10 mg/kg) respectively. Groups 3 and 4 were exposed to stress (sleep deprivation, predator exposure, immobility, rapid cage changes, noise, and foreign object) with group 4 concurrently supplemented with vitamin C (10 mg/kg). Serum cortisol, oxidative bio-markers, fetal weights and in-vitro contractile responses of excised uterine tissue to acetylcholine (Ach), oxytocin, calcium chloride (CaCl2), potassium chloride (KCl), diclofenac, and magnesium ions were determined. Results Malondialdehyde activity and cortisol were significantly increased in variable stress only exposed group when compared with control and vitamin C supplemented groups. Fetal body weights, superoxide dismutase and catalase activity were significantly reduced in variable stress only exposed group. Significantly impaired contractile responses to Ach, CaCl2 & KCl in variable stress only exposed group were modulated in vitamin C supplemented groups. Impaired contractile response to oxytocin was however not reversed. Relaxation responses to diclofenac and magnesium ions were statistically unaltered across groups. Conclusions Impaired fetal weights and uterine contractile activity to Ach, CaCl2 and KCl during LGVS was modulated by vitamin C supplementation. Impaired oxytocin contractile activity was however unreversed.

Vitamin C protects carboplatin-exposed oocytes from meiotic failure

2019 ◽  
Vol 25 (10) ◽  
pp. 601-613 ◽  
Author(s):  
Changyin Zhou ◽  
Xue Zhang ◽  
Yuwei Zhang ◽  
Xiayan ShiYang ◽  
Yu Li ◽  
...  
Keyword(s):  
Vitamin C ◽  
Oocyte Quality ◽  
Platinum Compound ◽  
Cortical Granules ◽  
Sperm Binding ◽  
Abnormal Distribution ◽  
Vitamin C Supplementation ◽  
Underlying Mechanisms

Abstract CBP (carboplatin) is a second-generation chemotherapeutic drug of platinum compound commonly applied in the treatment of sarcomas and germ cell tumours. Although it is developed to replace cisplatin, which has been proven to have a variety of side effects during cancer treatment, CBP still exhibits a certain degree of toxicity including neurotoxicity, nephrotoxicity, hematotoxicity and myelosuppression. However, the underlying mechanisms regarding how CBP influences the female reproductive system especially oocyte quality have not yet been fully determined. Here, we report that CBP exposure led to the oocyte meiotic defects by impairing the dynamics of the meiotic apparatus, leading to a remarkably aberrant spindle organisation, actin polymerisation and mitochondrial integrity. Additionally, CBP exposure caused compromised sperm binding and fertilisation potential of oocytes by due to an abnormal distribution of cortical granules and its component ovastacin. More importantly, we demonstrated that vitamin C supplementation prevented meiotic failure induced by CBP exposure and inhibited the increase in ROS levels, DNA damage accumulation and apoptotic incidence. Taken together, our findings demonstrate the toxic effects of CBP exposure on oocyte development and provide a potential effective way to improve the quality of CBP-exposed oocytes in vitro.

Accumulation of Adenosine 3′,5′-Monophosphate and Relaxation in the Rat Uterus In Vitro

1971 ◽  
Vol 49 (11) ◽  
pp. 999-1004 ◽  
Author(s):  
Ivo Polacek ◽  
Jean Bolan ◽  
Edwin E. Daniel
Keyword(s):  
Cyclic Amp ◽  
Contractile Activity ◽  
Phosphodiesterase Activity ◽  
Dibutyryl Camp ◽  
Rat Uterus ◽  
Low Concentrations ◽  
Uterine Contractile ◽  
Camp Levels

Theophylline, diazoxide, and papaverine in low concentrations relaxed the uterus with minimal or no elevation of cyclic AMP (cAMP) levels. In higher concentrations, theophylline relaxed the uterus and increased its cAMP levels, but imidazole reversed the increase in cAMP without causing recontraction. Imidazole and NaF caused uterine contractures but did not detectably decrease cAMP levels until several minutes after the onset of contractures. The uterine relaxations produced by theophylline and/or dibutyryl cAMP in amounts which increased uterine cAMP were not reversed by propranolol. These results eliminate the possibility that propranolol interfered with a relaxant action of cAMP. Along with previous data, these results also show that uterine contractile activity was not determined primarily by the general levels of cAMP and that phosphodiesterase activity in the uterus was insufficient to rapidly affect these cAMP levels. Also, substances like theophylline, diazoxide, and papaverine, postulated to inhibit phosphodiesterase activity, did not bring about their relaxant effects by this mechanism.

scholarly journals Экспериментальные модели сократительной активности миометрия

2020 ◽  
Author(s):  
A.A. Yakovleva ◽  
N.G. Pavlova
Keyword(s):  
In Silico ◽  
Contractile Activity ◽  
Experimental Models ◽  
Pacemaker Activity ◽  
Single View ◽  
Uterine Contractile ◽  
Experimental Approaches

Сократительная деятельность матки до настоящего времени остается актуальным вопросом фундаментальных исследований, поскольку отсутствуют единые представления о биомеханике маточного сокращения, необходимые для профилактики родового и акушерского травматизма. Цель работы - оценка ограничений и возможностей экспериментальных моделей, предназначенных для изучения сократительной активности миометрия. Методика. Основными экспериментальными подходами к изучению сократительной активности матки являлись исследования in vitro, in situ, in vivo, in silico, а также их сочетание. Результаты. В статье рассмотрены исследования, в которых использованы различные сочетания экспериментальных подходов, обсуждаются результаты, полученные при моделировании в экспериментах, обсуждаются результаты изучения синхронизации сокращения отделов матки на различных моделях, а также результаты исследования пейсмейкерной активности миометрия и возможность экстраполяции полученных данных на человека. В связи с активным развитием компьютерных технологий в статье поднимается вопрос об их использовании в моделировании сократительной активности матки человека. Заключение. Делается заключение, что комплексный подход, включающий электромиографические, биохимические и морфологические исследования в хроническом эксперименте, является наиболее адекватным для изучения сократительной активности миометрия и функционального состояния нормально развитых и отставших в развитии плодов, что позволит разработать комплексные методы профилактики родового и акушерского травматизма.Uterine contractile activity remains an important issue of fundamental research as there is no single view of the biomechanics of uterine contraction necessary for the prevention of parturition and obstetric trauma until now. The aim of the review was to assess advantages and limitations of experimental models described in the literature for study uterine contractile activity. At the present time main experimental approaches for study myometrium contractile activity are research in vitro, in situ, in vivo, in silico and the their combinations. The literature presents experimental approaches, different models of uterine contractions synchronization and study of myometrium pacemaker activity. Due to active development of computer technologies there is a need to model human uterine contractile activity with a simplified anatomy. The authors propose that combination of electromyographic, biochemical and morphological methods in chronic experiment is the most correct and appropriate direction for the assessment of the myometrium contractile activity and functional state of normally developed and growth restricted fetuses.

Abnormal labor as a medical and social issue

2021 ◽  
Vol 70 (5) ◽  
pp. 117-130
Author(s):  
Viktor A. Mudrov
Keyword(s):  
Social Issue ◽  
Social Issues ◽  
Inflammatory Diseases ◽  
Contractile Activity ◽  
Cochrane Library ◽  
Perinatal Complications ◽  
Uterine Contractility ◽  
Systematic Analysis ◽  
Fetal Asphyxia ◽  
Uterine Contractile

Abnormal labor is a main cause of obstetric and perinatal complications. Impaired uterine contraction during childbirth is accompanied by an increase in the risk of fetal asphyxia, obstetric bleeding and postpartum inflammatory diseases. The frequency of diagnosed abnormal uterine contractile activity is 10-25%, which, along with the high need for operative delivery, allows for considering abnormal labor as one of the main medical and social issues of the present time. The aim of this study was to consider abnormal labor as a medical and social problem. This was achieved by using an analytical method including carrying out a detailed systematic analysis of modern domestic and foreign literature on abnormal labor. The study used databases such as eLIBRARY.RU, Scopus, PubMed, MEDLINE, ScienceDirect, and Cochrane Library from the creation until July 2021. Abnormal labor is an important medical and social issue that requires the active development of methods to predict and prevent not only violations of uterine contractility, but also their complications.

scholarly journals Complex Effects of Vitamin E and Vitamin C Supplementation on in Vitro Neonatal Mononuclear Cell Responses to Allergens

Nutrients ◽  
2013 ◽  
Vol 5 (9) ◽  
pp. 3337-3351 ◽  
Author(s):  
Heather Wassall ◽  
Graham Devereux ◽  
Anthony Seaton ◽  
Robert Barker
Keyword(s):  
Vitamin E ◽  
Vitamin C ◽  
Mononuclear Cell ◽  
Cell Responses ◽  
Vitamin C Supplementation

scholarly journals Modelling maternal obesity: the effects of a chronic high-fat, high-cholesterol diet on uterine expression of contractile-associated proteins and ex vivo contractile activity during labour in the rat

2015 ◽  
Vol 130 (3) ◽  
pp. 183-192 ◽  
Author(s):  
Ronan Muir ◽  
Jean Ballan ◽  
Bethan Clifford ◽  
Sarah McMullen ◽  
Raheela Khan ◽  
...  
Keyword(s):  
Maternal Obesity ◽  
Ex Vivo ◽  
Contractile Activity ◽  
High Cholesterol Diet ◽  
Obese Women ◽  
Uterine Contractility ◽  
High Cholesterol ◽  
Steroid Synthesis ◽  
Uterine Contractile ◽  
Associated Proteins

Modelling maternal obesity in rats adversely affected steroid synthesis, uterine contractile associated protein expression and ex-vivo uterine contractility during labour. This maternal obesity model can be utilized further to unravel the mechanisms causing uterine dystocia in obese women.

Genistein and daidzein modulate in vitro rat uterine contractile activity

2000 ◽  
Vol 75 (2-3) ◽  
pp. 201-208 ◽  
Author(s):  
C Picherit ◽  
M Dalle ◽  
G Néliat ◽  
P Lebecque ◽  
M.J Davicco ◽  
...  
Keyword(s):  
Contractile Activity ◽  
Uterine Contractile

Effects of β-adrenergic agonist infusions on myometrial activity and plasma prostaglandin levels in the nonpregnant sheep

1987 ◽  
Vol 65 (11) ◽  
pp. 2297-2301 ◽  
Author(s):  
S. J. Lye ◽  
P. Christopher ◽  
R. F. Casper
Keyword(s):  
Contractile Activity ◽  
Vena Cava ◽  
Emg Activity ◽  
Adrenergic Agonists ◽  
Adrenergic Agonist ◽  
Uterine Contractions ◽  
Uterine Contractile ◽  
Differential Increase

Recent studies have reported that β-adrenergic agonists stimulate the production of stimulatory prostaglandins (PGs) by intrauterine tissues in vitro. These drugs are used clinically to inhibit uterine contractions; consequently an increase in stimulatory PGs in vivo might have potentially adverse effects. We have, therefore, investigated whether β-adrenergic agonists increase plasma PG concentrations in vivo. Samples of peripheral (aorta) and uterine venous enriched (vena cava) blood from nonpregnant sheep were collected at 15-min intervals for 1 h before, 3 h during, and 1 h postinfusion of either (a) the β-adrenergic agonist isoproterenol (Isop) at a dose of 0.16 μg∙kg−1 min−1; (b) Isop at a dose of 0.08 μg∙kg−1∙min−1; or (c) saline, 1 mL/h via a jugular vein catheter. The sheep were also equipped with intrauterine recording balloons to record intrauterine pressure and myometrial electromyographic (EMG) electrodes to measure EMG activity. Infusion of Isop at 0.16 μg∙kg−1∙min−1 produced a significant initial inhibition of uterine activity, although contractions returned (within 60 min) despite continued administration of Isop. Plasma PGE2 (but not PGF2α or 13,14-dihydro-15-keto-PGF2α (PGFM)) concentrations were significantly elevated during the Isop infusion. Administration of Isop at 0.08 μg∙kg−1∙min−1 produced no effects on uterine contractile activity but was associated with a significant elevation in plasma PGE2 (but not PGF2α or PGFM) concentrations. No changes in plasma PGE2, PGF2α, or PGFM occurred during saline infusion. The data suggest that β-adrenergic agonist infusion is associated with a differential increase in plasma PG levels in vivo, but that this effect is probably not related to the failure of Isop (0.16 μg∙kg−1∙min−1) to maintain inhibition of myometrial contractions.

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