DNA analysis : Current status in court

2004 ◽  
Vol 1 (3) ◽  
Author(s):  
W. J. Wall
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Current Status ◽  
Str Analysis ◽  
Dna Profile ◽  
Single Locus Probes ◽  
Or Groups ◽  
Starting Point ◽  
Short Tandem

AbstractSince the introduction of DNA profiling the methods have change considerably and so have the way that results are expressed. There have been many different techniques used to produce a DNA profile. The starting point was multilocus probes, followed by single locus probes, neither of which are now widely used, and then Short tandem Repeat (STR) analysis. Whatever the next step in DNA analysis is, the methods of interpretation will remain essentially the same. We rely on the concept and reality of the benign state for this to be used for the good of society, not the oppression of individuals or groups.

scholarly journals Short tandem repeat typing technologies used in paternity testing: a case study

2020 ◽  
pp. 04-09
Author(s):  
Amany Abdel Hakim Mousa ◽  
Dena Mohamed Naguib Abdel Moawed ◽  
Adel Mohamed Abdallah Elberry
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Nuclear Dna ◽  
Pcr Amplification ◽  
Str Typing ◽  
Biological Father ◽  
Str Analysis ◽  
Dna Profile ◽  
Federal Bureau Of Investigation ◽  
Short Tandem

Background: In forensic field, for establishing the paternity of disputed offspring, STR typing (autosomal, and Y typing) are commonly used to dissolve the cases. Short tandem repeat (STR) technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another. The Federal Bureau of Investigation (FBI) uses a standard set of 13 specific STR regions for CODIS. Aim of the study: The present work was done to find out the biological father of child in a case where mother was killed by her husband for the doubt of her pregnancy. Methods: After taking blood samples from the murdered mother, the baby and the killer husband, Autosomal STRs Using AmpFlSTR Identifiler Plus PCR Amplification Kit and Y-STR analysis using AmpFlSTR Yfiler PCR Amplification Kit were done to solve the disputed paternity case. Results: After the analysis of DNA STRs profiles of autosomal and Y-chromosome markers, the dead fetus was proved to be the son of the killer father. Conclusions: Autosomal STR multiplex analysis combined with Y-STR analysis help to reach a conclusion in disputed paternity cases with greater confidence. Keywords: Disputed; Paternity; Multiplex; Short tandem reprats; Y-STR; Analysis

Evaluation genotypes of cancer cell lines HCC1954 and SiHa by short tandem repeat (STR) analysis and DNA sequencing

2018 ◽  
Vol 45 (6) ◽  
pp. 2689-2695 ◽  
Author(s):  
Jiewen Fu ◽  
Jingliang Cheng ◽  
Xiaoyan Liu ◽  
Jun Li ◽  
Chunli Wei ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Cancer Cell Lines ◽  
Str Analysis ◽  
Short Tandem

DNA analysis in disputed parentage: The occurrence of two apparently false exclusions of paternity, both at short tandem repeat (STR) loci, in the one child

1997 ◽  
Vol 18 (9) ◽  
pp. 1650-1652 ◽  
Author(s):  
Peter R. Gunn ◽  
Katherine Trueman ◽  
Patricia Stapleton ◽  
Derryck B. Klarkowski
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Dna Analysis ◽  
Str Loci ◽  
The One ◽  
Short Tandem

Variant alleles observed in short tandem repeat (STR) analysis of random Indian population

2019 ◽  
Vol 19 (1) ◽  
pp. 94
Author(s):  
Monika Chakravarty ◽  
Nidhi Singh ◽  
Damini Varshney ◽  
Dhruw Sharma ◽  
Naresh Kumar ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Indian Population ◽  
Str Analysis ◽  
Variant Alleles ◽  
Short Tandem

Automated short tandem repeat (STR) analysis in forensic casework — a strategy for the future

1995 ◽  
Vol 16 (1) ◽  
pp. 1543-1552 ◽  
Author(s):  
Peter Gill ◽  
Colin P. Kimpton ◽  
Andrew Urquhart ◽  
Nicola Oldroyd ◽  
Emma S. Millican ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Str Analysis ◽  
Forensic Casework ◽  
The Future ◽  
Short Tandem

scholarly journals Research Resource: STR DNA Profile and Gene Expression Comparisons of Human BG-1 Cells and a BG-1/MCF-7 Clonal Variant

2014 ◽  
Vol 28 (12) ◽  
pp. 2072-2081 ◽  
Author(s):  
Yin Li ◽  
Yukitomo Arao ◽  
Julie M. Hall ◽  
Sandra Burkett ◽  
Liwen Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Cell Line ◽  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Expression Patterns ◽  
Genotypic Differences ◽  
Dna Profile ◽  
Short Tandem ◽  
Mcf 7

Human ovarian cancer BG-1 cells are a valuable in vitro model that has enabled several laboratories to study the estrogenic responses of ovarian cancers. We recently discovered that there are two different BG-1 cell lines being used for experiments, denoted here as BG-1 FR and BG-1 NIEHS, which exhibit striking morphological differences. The objective of this study was to methodically analyze these two BG-1 variants and compare their characteristics. Short tandem repeat analysis revealed that the DNA profile of BG-1 FR cells was unique, yet the Short tandem repeat pattern of BG-1 NIEHS was identical with that of MCF-7 cells. From a cytogenetic analysis, it became apparent that the BG-1 FR line had the same profile as previously reported, whereas the BG-1 NIEHS and MCF-7 cells share a similar genetic display. A significant number of unique chromosomal translocations were observed between the BG-1 NIEHS and MCF-7 cells, suggesting that acquired genotypic differences resulted in the formation of two lines from a common origin. Although all cell types demonstrated a similar estrogen responsiveness in reporter gene assays, a microarray analysis revealed distinct estrogen-responsive gene expression patterns with surprisingly moderate to low overlap. We conclude that BG-1 FR is the original ovarian cancer cell line, whereas the BG-1 NIEHS is a variant from the MCF-7 cells. These findings provide much needed clarification of the identities and characteristics of key cell line models that are widely used to study estrogen action in female reproductive cancers.

scholarly journals Utilization of Short Tandem Repeat Analysis to Resolve Specimen Mislabeling in the Anatomic Pathology Laboratory

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S112-S112
Author(s):  
T Tenney ◽  
B Robinson ◽  
H C Sullivan
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Single Patient ◽  
Pathology Laboratory ◽  
Str Analysis ◽  
Hematopoietic Stem ◽  
Anatomic Pathology ◽  
The Given ◽  
Short Tandem

Abstract Introduction/Objective A mislabeled specimen is an example of preanalytical error that can have significant consequences on patient care. These errors can be difficult to detect and resolve. One method to confirm genetic identity is short tandem repeat (STR) analysis, which is utilized in forensic investigations, paternity studies, and post- hematopoietic stem cell transplantation monitoring. Herein we present application of STR analysis to resolve a suspected specimen mislabeling prior to receipt in our anatomic pathology laboratory. Methods/Case Report DNA was extracted from paraffin embedded tissues. Chimerism testing was performed by STR analysis using the Globalfiler (ThermoFisher Scientific) and analyzed by Chimermarker (Softgenetics) automated chimerism software. Results (if a Case Study enter NA) Colon biopsies were received for a single patient (#1) with two requisition forms. Each specimen (A-F) was labeled with the patient’s name, with specimens A-D noted on first page of requisition and specimens E-F on the second requisition page. After the case was signed out, the lab was contacted looking for biopsy results on another patient (#2) who was seen on the same day as patient #1. Review of all the patients seen in the endoscopy suite on the given date raised suspicion that specimens E-F from the second page of the requisition actually pertained to patient #2. STR analysis performed on specimens confirmed that specimens E-F were genetically distinct from those labelled A-D. Tissue from a subsequent biopsy on patient #2 was analyzed by STR testing, which was identical to STR results performed on specimens E-F. Conclusion Here we utilized STR testing to resolve a suspected mislabeled specimen, allowing the appropriate diagnosis to be attributed to the correct patient. This is a unique application of a common method, which could be implemented in anatomic pathology laboratories to resolve cases of specimen mix-ups.

scholarly journals Individual Identification with Short Tandem Repeat Analysis and Collection of Secondary Information Using Microbiome Analysis

Genes ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 85
Author(s):  
Solip Lee ◽  
Heesang You ◽  
Songhee Lee ◽  
Yeongju Lee ◽  
Hee-Gyoo Kang ◽  
...  
Keyword(s):  
Tandem Repeat ◽  
Short Tandem Repeat ◽  
Individual Identification ◽  
Future Research ◽  
Str Analysis ◽  
Legal Evidence ◽  
Advantages And Disadvantages ◽  
Secondary Information ◽  
Generation Sequencing ◽  
Short Tandem

Forensic investigation is important to analyze evidence and facilitate the search for key individuals, such as suspects and victims in a criminal case. The forensic use of genomic DNA has increased with the development of DNA sequencing technology, thereby enabling additional analysis during criminal investigations when additional legal evidence is required. In this study, we used next-generation sequencing to facilitate the generation of complementary data in order to analyze human evidence obtained through short tandem repeat (STR) analysis. We examined the applicability and potential of analyzing microbial genome communities. Microbiological supplementation information was confirmed for two of four failed STR samples. Additionally, the accuracy of the gargle sample was confirmed to be as high as 100% and was highly likely to be classified as a body fluid sample. Our experimental method confirmed that anthropological and microbiological evidence can be obtained by performing two experiments with one extraction. We discuss the advantages and disadvantages of using these techniques, explore prospects in the forensic field, and highlight suggestions for future research.

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