scholarly journals Diminished HLA-DR expression on monocyte and dendritic cell subsets indicating impairment of cellular immunity in pre-term neonates: a prospective observational analysis

2015 ◽  
Vol 43 (5) ◽  
Author(s):  
Joerg C. Schefold ◽  
Linn Porz ◽  
Barbara Uebe ◽  
Holger Poehlmann ◽  
Stephan von Haehling ◽  
...  
Keyword(s):  
Gestational Age ◽  
Human Leukocyte ◽  
Severe Infection ◽  
Full Term ◽  
Term Neonates ◽  
Observational Analysis ◽  
Leukocyte Antigen ◽  
Hla Dr ◽  
Leukocyte Counts ◽  
Antigen Presenting

AbstractThe risk of neonates for severe infection/sepsis is reciprocally proportional to gestational age and birth weight. As monocytes and dendritic cells (DC) are recognised key antigen-presenting immune cells, we aimed to elucidate whether neonatal age is associated with reduced expression of human-leukocyte antigen-DR (HLA-DR) antigens on subsets of monocytes and DCs.Forty-three consecutive neonates (20 male, mean gestational age 236.0±26.8 days; mean 1-min Apgar score 7.5±2.0) were included in a monocentric prospective observational analysis. Patients were grouped according to gestational age (n=15 full-term, n=28 pre-term defined as <33 weeks). Ten healthy adult volunteers were assessed also. Flow-cytometric assessment of HLA-DR expression was performed in subsets of peripheral blood myeloid and plasmacytoid DCs (MDC and PDC) and monocytes (CD14At birth, leukocyte counts were increased in full-term neonates. Monocyte counts were significantly increased in neonates when compared with adults (all P<0.05). A significant numerical increase of CD14We observed a markedly diminished HLA-DR expression on monocyte and DC subsets in pre-term and full-term neonates, which may contribute to impaired antimicrobial defence mechanisms in the early days of life.

Endogenous NGF regulates CGRP expression in human monocytes, and affects HLA-DR and CD86 expression and IL-10 production

Blood ◽  
2005 ◽  
Vol 106 (10) ◽  
pp. 3507-3514 ◽  
Author(s):  
Luisa Bracci-Laudiero ◽  
Luigi Aloe ◽  
Maria Cristina Caroleo ◽  
Pasquale Buanne ◽  
Nicola Costa ◽  
...  
Keyword(s):  
Mhc Class Ii ◽  
T Cell Activation ◽  
Cell Activation ◽  
Human Leukocyte ◽  
Interleukin 10 ◽  
Human Monocytes ◽  
Leukocyte Antigen ◽  
Physiologic Mechanism ◽  
Hla Dr ◽  
Antigen Presenting

AbstractOur recent results on autocrine nerve growth factor (NGF) synthesis in B lymphocytes, which directly regulates the expression and release of calcitonin gene-related peptide (CGRP), a neuropeptide known to down-regulate immune response, led us to propose an anti-inflammatory action of NGF. In the present work, we investigated whether the endogenous synthesis of NGF can regulate the expression of CGRP in other antigen-presenting cells, such as monocytes, and whether this may have a functional effect. Our data indicate that human monocytes synthesize basal levels of NGF and CGRP and that, following lipopolysaccharide (LPS) stimulation, NGF and CGRP expression are both up-regulated. When endogenous NGF is neutralized, the up-regulation of CGRP expression induced by LPS is inhibited. The expression of membrane molecules involved in T-cell activation such as human leukocyte antigen-DR (HLA-DR) and CD86 is affected by endogenous NGF, and similar effects were obtained using a CGRP1 receptor antagonist. In addition, NGF deprivation in LPS-treated monocytes significantly decreases interleukin 10 (IL-10) synthesis. Our findings indicate that endogenous NGF synthesis has a functional role and may represent a physiologic mechanism to down-regulate major histocompatibility complex (MHC) class II and CD86 expression and alter the development of immune responses.

scholarly journals Myeloid phenotypes in severe COVID-19 predict secondary infection and mortality: a pilot study

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Clémence Marais ◽  
Caroline Claude ◽  
Nada Semaan ◽  
Ramy Charbel ◽  
Simon Barreault ◽  
...  
Keyword(s):  
Critically Ill ◽  
Host Response ◽  
Secondary Infection ◽  
Myeloid Cells ◽  
Suppressor Cells ◽  
Human Leukocyte ◽  
Myeloid Derived Suppressor Cells ◽  
Phenotypic Characteristics ◽  
Leukocyte Antigen ◽  
Hla Dr

Abstract Background De-regulated host response to severe coronavirus disease 2019 (COVID-19), directly referring to the concept of sepsis-associated immunological dysregulation, seems to be a strong signature of severe COVID-19. Myeloid cells phenotyping is well recognized to diagnose critical illness-induced immunodepression in sepsis and has not been well characterized in COVID-19. The aim of this study is to review phenotypic characteristics of myeloid cells and evaluate their relations with the occurrence of secondary infection and mortality in patients with COVID-19 admitted in an intensive care unit. Methods Retrospective analysis of the circulating myeloid cells phenotypes of adult COVID-19 critically ill patients. Phenotyping circulating immune cells was performed by flow cytometry daily for routine analysis and twice weekly for lymphocytes and monocytes subpopulations analysis, as well as monocyte human leukocyte antigen (mHLA)-DR expression. Results Out of the 29 critically ill adult patients with severe COVID-19 analyzed, 12 (41.4%) developed secondary infection and six patients died during their stay. Monocyte HLA-DR kinetics was significantly different between patients developing secondary infection and those without, respectively, at day 5–7 and 8–10 following admission. The monocytes myeloid-derived suppressor cells to total monocytes ratio was associated with 28- and 60-day mortality. Those myeloid characteristics suggest three phenotypes: hyperactivated monocyte/macrophage is significantly associated with mortality, whereas persistent immunodepression is associated with secondary infection occurrence compared to transient immunodepression. Conclusions Myeloid phenotypes of critically ill COVID-19 patients may be associated with development of secondary infection, 28- and 60-day mortality.

Hypoglycaemia and seizures in large-for-gestational-age (LGA) full-term neonates

2007 ◽  
Vol 95 (7) ◽  
pp. 874-876
Author(s):  
Floris Groenendaal ◽  
Patty M. Elferink-Stinkens ◽  
Keyword(s):  
Gestational Age ◽  
Full Term ◽  
Large For Gestational Age ◽  
Term Neonates

scholarly journals Sampling From the Proteome to the Human Leukocyte Antigen-DR (HLA-DR) Ligandome ProceedsViaHigh Specificity

2016 ◽  
Vol 15 (4) ◽  
pp. 1412-1423 ◽  
Author(s):  
Geert P. M. Mommen ◽  
Fabio Marino ◽  
Hugo D. Meiring ◽  
Martien C. M. Poelen ◽  
Jacqueline A. M. van Gaans-van den Brink ◽  
...  
Keyword(s):  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Hla Dr

scholarly journals Inflammatory Molecules in Aqueous Humour and on Ocular Surface and Glaucoma Surgery Outcome

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Barbara Cvenkel ◽  
Andreja Nataša Kopitar ◽  
Alojz Ihan
Keyword(s):  
Ocular Surface ◽  
Aqueous Humour ◽  
Human Leukocyte ◽  
Glaucoma Surgery ◽  
Antiglaucoma Medication ◽  
Surgery Outcome ◽  
Hla Dr ◽  
Antigen Presenting ◽  
Inflammatory Milieu ◽  
Inflammatory Molecules

Purpose. To investigate the influence of inflammatory molecules in the aqueous humour and on the ocular surface on the outcome of glaucoma surgery.Methods. Thirty patients who needed antiglaucomatous surgery were included. The interleukin- (IL-) 8, IL-1, IL-6, IL-10, tumour necrosis factor- (TNF-) ; and IL-12 were determined from aqueous humour preoperatively and the imprints of conjunctiva were analysed for expression of human leukocyte antigen- (HLA-)-DR after surgery by flow cytometry. The success of trabeculectomy was defined as intraocular pressure less than 21 mmHg without antiglaucoma medication.Results. Eyes with trabeculectomy failure at 3 months showed significantly higher TNF- and IL-6 levels in the aqueous than eyes with successful surgery. Increased expression of HLA-DR on epithelial cells and antigen-presenting cells was not associated with the trabeculectomy outcome.Conclusions. Higher preoperative levels of TNF- and IL-6 in aqueous humour may contribute to the development of inflammatory milieu and were associated with worse outcome of glaucoma surgery.

Human Leukocyte Antigen (HLA)-Linked Control of Susceptibility to Pulmonary Tuberculosis and Association with HLA-DR Types

1983 ◽  
Vol 148 (4) ◽  
pp. 676-681 ◽  
Author(s):  
S. P. N. Singh ◽  
N. K. Mehra ◽  
H. B. Dingley ◽  
J. N. Pande ◽  
M. C. Vaidya
Keyword(s):  
Pulmonary Tuberculosis ◽  
Human Leukocyte Antigen ◽  
Human Leukocyte ◽  
Leukocyte Antigen ◽  
Hla Dr

Additive effect of the HLA-DR15 haplotype on susceptibility to multiple sclerosis

2001 ◽  
Vol 7 (2) ◽  
pp. 91-93 ◽  
Author(s):  
H B Rasmussen ◽  
M A Kelly ◽  
J Clausen
Keyword(s):  
Multiple Sclerosis ◽  
Meta Analysis ◽  
Human Leukocyte ◽  
Surface Expression ◽  
Relative Effect ◽  
European Origin ◽  
Future Studies ◽  
Leukocyte Antigen ◽  
Density Surface ◽  
Antigen Presenting

Multiple sclerosis (MS) has been associated with the human leukocyte antigen DR15 allele in Caucasians of North and Central European origin. However, the relative effect of the DR15 homozygous and the DR15 heterozygous genotypes on the disease susceptibility is unclear. Based upon results from three North European studies we have examined this by meta-analysis. Our results suggested that the effect of the DRB1*1501, DQA1*0102, DQB1*0602 haplotype on the susceptibility to MS is additive, perhaps reflecting that development of the disease is facilitated by a high density surface expression of the antigen presenting molecules encoded by this haplotype. Possible implications of our finding to future studies of the genetic background of MS is discussed.

Clot formation of neonates tested by thromboelastography correlates with gestational age

2010 ◽  
Vol 103 (02) ◽  
pp. 344-350 ◽  
Author(s):  
Tzipi Strauss ◽  
Yael Levy-Shraga ◽  
Bruria Ravid ◽  
Irit Schushan-Eisen ◽  
Ayala Maayan-Metzger ◽  
...  
Keyword(s):  
Cord Blood ◽  
Gestational Age ◽  
Blood Clot ◽  
Full Term ◽  
Clot Formation ◽  
Clotting Time ◽  
Term Infants ◽  
Term Neonates ◽  
Pregnancy And Delivery ◽  
Clot Formation Time

SummaryEvaluation of clot formation in neonates is troublesome. Our aim was to investigate cord blood clot formation of pre-term versus full-term infants and adults, using rotating thromboelastogram (ROTEM®, Pentafarm, Munich, Germany). ROTEM was investigated in cord blood of 184 full-term and 47 pre-term infants. Measurements of the clotting time (CT), clot formation time (CFT) and maximal clot firmness (MCF) were obtained in order to asses reference values for this age group, and compare between full-term and pre-term neonates and compared to adult controls. For each infant demographic information and data regarding pregnancy and delivery were gathered. Infants were prospectively followed until discharge. CT and CFT were significantly shorter among pre-term and term infants as compared to adults [median CT: 185, 194, 293 seconds respectively, p≤0.001, CFT: 80, 76, 103 seconds respectively, p≤0.001). MCF was lower in pre-term and term as compared to adults (p≤0.001) with significantly lower values in pre-term as compared to full-term neonates (p=0.004). Clotting time and MCF correlated with gestational age (R=0.132, p=0.045, R= 0.259, p<0.001, respectively). No association was found between any ROTEM values and the occurrence of post-natal complications in infants of our study group. This is the first study assessing clot formation by ROTEM in pre-term infants. Clot formation parameters of term and premature infants correlated with gestational age. The predictive value of clot formation tests in neonates deserves further attention.

scholarly journals Increased CD69 and Human Leukocyte Antigen-DR Expression on T Lymphocytes in Insulin-Dependent Diabetes Mellitus of Long Standing

1998 ◽  
Vol 83 (6) ◽  
pp. 2204-2209 ◽  
Author(s):  
Alois Gessl ◽  
Werner Waldhäusl
Keyword(s):  
Diabetes Mellitus ◽  
T Cells ◽  
Human Leukocyte ◽  
Insulin Dependent Diabetes Mellitus ◽  
Dependent Diabetes Mellitus ◽  
Cd4 Cells ◽  
Cd8 Cells ◽  
Leukocyte Antigen ◽  
Hla Dr ◽  
Insulin Dependent

To better define prevailing activation of circulating T cell subsets in insulin-dependent diabetes mellitus (IDDM) of recent onset (DM; n= 31; median age ± sd,, 28 ± 6.9 yr) and of long standing (DML; n = 27; age, 33 ± 10.4 yr; median duration of disease, 105 months), CD4+ and CD8+ T cells were analyzed to determine their naive and memory subsets as well as their expression of human leukocyte antigen (HLA)-DR, interleukin-2 receptor α-chain (CD25), and CD69 by three-color flow cytometry. Twenty-six healthy subjects (HS; age, 32.0 ± 8.2 yr) served as controls. No deviation was seen in either IDDM group compared to HS in CD25 expression on CD4+ or CD8+ cells or in their CD45RA+ or CD45RA− subsets. HLA-DR expression, however, was increased (P &lt; 0.05) in total CD8+ cells and CD45RA+ cells, with CD45RA− CD8+ cells joining the prevailing pattern only in DML. Among CD4+ cells, increased expression of HLA-DR molecules was restricted to total and CD45RA− cells in DML. CD69 expression did not differ between IDDM and HS, but differed between DML (CD4+, CD8+, and CD45RA− CD4+) and DM only. In conclusion, our data demonstrate that HLA-DR expression in IDDM is restricted to memory cells (CD45RA−) among CD4+ cells in DML and is more markedly confined to naive (CD45RA+) than to memory CD8+ cells, whereas the early activation antigen CD69 is more readily expressed in DML than in DM. The observed activation of circulating T cells suggests an ongoing immune process in IDDM both at clinical manifestation and after long duration.

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