Einfluß von Probengewinnung und Lagerungsbedingungen auf die Stabilität des totalen und freien prostata-spezifischen Antigens. Influence of Sample Collection and Storage Conditions on the Stability of Total and Free Prostate-specific Antigen

Abstract Background: The accuracy of homocysteine (Hcy) results is currently compromised by the requirement to separate the plasma within 1 h of sample collection. We studied the effect of temperature on the stability of plasma Hcy over a 72-h time course in blood collected into evacuated tubes containing either EDTA alone or both EDTA and 3-deazaadenosine (3DA). Methods: We recruited 100 volunteers, including both diseased and healthy individuals with a range of baseline plasma Hcy values, from two centers. Blood samples were collected into tubes containing EDTA, and EDTA plus 3DA and stored at ambient temperature (20–25 °C) or refrigerated (2–8 °C). Aliquots of blood were centrifuged at various times up to 72 h, the plasma was removed, and Hcy was measured by HPLC. Results: Plasma Hcy measurement covering the sample collection and storage conditions during the whole time course was possible on samples from 59 of those recruited. One-way ANOVA for repeated measures within subjects revealed that only samples that were collected into tubes containing EDTA plus 3DA and stored refrigerated were stable over 72 h (P = 0.2761). Conclusions: A combination of 3DA and storage at 2–8 °C will allow collection of samples for plasma Hcy measurement outside of the hospital setting and wider population screening.

Download Full-text

Different Stability of Free and Complexed Prostate-Specific Antigen in Serum in Relation to Specimen Handling and Storage Conditions

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm.2000.200 ◽

2000 ◽

Vol 38 (12) ◽

Cited By ~ 10

Author(s):

Klaus Jung ◽

Michael Lein ◽

Brigitte Brux ◽

Pranav Sinha ◽

Dietmar Schnorr ◽

...

Keyword(s):

Prostate Specific Antigen ◽

Specific Antigen ◽

Storage Conditions ◽

Specimen Handling ◽

And Storage

Download Full-text

Effect of pH and temperature on the infectivity of human coronavirus 229E

Canadian Journal of Microbiology ◽

10.1139/m89-160 ◽

1989 ◽

Vol 35 (10) ◽

pp. 972-974 ◽

Cited By ~ 40

Author(s):

Alain Lamarre ◽

Pierre J. Talbot

Keyword(s):

Incubation Period ◽

Storage Conditions ◽

Viral Infectivity ◽

Human Coronavirus ◽

Virus Growth ◽

The Stability ◽

And Storage ◽

Influence Of Ph ◽

Human Coronavirus 229E

The stability of human coronavirus 229E infectivity was maximum at pH 6.0 when incubated at either 4 or 33 °C. However, the influence of pH was more pronounced at 33 °C. Viral infectivity was completely lost after a 14-day incubation period at 22, 33, or 37 °C but remained relatively constant at 4 °C for the same length of time. Finally, the infectious titer did not show any significant reduction when subjected to 25 cycles of thawing and freezing. These studies will contribute to optimize virus growth and storage conditions, which will facilitate the molecular characterization of this important pathogen.Key words: coronavirus, pH, temperature, infectivity, human coronavirus.

Download Full-text

Pre-Drawn Syringes of Comirnaty for an Efficient COVID-19 Mass Vaccination: Demonstration of Stability

Pharmaceutics ◽

10.3390/pharmaceutics13071029 ◽

2021 ◽

Vol 13 (7) ◽

pp. 1029

Author(s):

Francesca Selmin ◽

Umberto M. Musazzi ◽

Silvia Franzè ◽

Edoardo Scarpa ◽

Loris Rizzello ◽

...

Keyword(s):

Mechanical Stress ◽

Real Life ◽

Storage Conditions ◽

Lipid Nanoparticles ◽

Mass Vaccination ◽

Hydrodynamic Diameter ◽

The Us ◽

Real Mass ◽

The Stability ◽

And Storage

Moving towards a real mass vaccination in the context of COVID-19, healthcare professionals are required to face some criticisms due to limited data on the stability of a mRNA-based vaccine (Pfizer-BioNTech COVID-19 Vaccine in the US or Comirnaty in EU) as a dose in a 1 mL-syringe. The stability of the lipid nanoparticles and the encapsulated mRNA was evaluated in a “real-life” scenario. Specifically, we investigated the effects of different storing materials (e.g., syringes vs. glass vials), as well as of temperature and mechanical stress on nucleic acid integrity, number, and particle size distribution of lipid nanoparticles. After 5 h in the syringe, lipid nanoparticles maintained the regular round shape, and the hydrodynamic diameter ranged between 80 and 100 nm with a relatively narrow polydispersity (<0.2). Samples were stable independently of syringe materials and storage conditions. Only strong mechanical stress (e.g., shaking) caused massive aggregation of lipid nanoparticles and mRNA degradation. These proof-of-concept experiments support the hypothesis that vaccine doses can be safely prepared in a dedicated area using an aseptic technique and transferred without affecting their stability.

Download Full-text

The Stability of Prostate-Specific Antigen in Semen Under Various Temperatures

Journal of Forensic Sciences ◽

10.1111/1556-4029.12791 ◽

2015 ◽

Vol 60 (6) ◽

pp. 1577-1581 ◽

Cited By ~ 2

Author(s):

Supawon Srettabunjong ◽

Parimol Betset ◽

Suvit Limawongpranee ◽

Pattama Ekpo

Keyword(s):

Prostate Specific Antigen ◽

Specific Antigen ◽

The Stability

Download Full-text

THE DEVELOPMENT OF A METHOD FOR DIRECT MASS SPECTROMETRIC ANALYSIS OF BIOLOGICAL SAMPLES USING POROUS SAMPLERS

Aerospace and Environmental Medicine ◽

10.21687/0233-528x-2021-55-1-99-103 ◽

2021 ◽

Vol 55 (1) ◽

pp. 99-103

Author(s):

R.E. Levin ◽

◽

M.A. Shamraeva ◽

I.M. Larina ◽

D.S. Bormotov ◽

...

Keyword(s):

Biological Samples ◽

Biological Fluids ◽

Storage Conditions ◽

Spectrometric Analysis ◽

Sample Collection ◽

Mucous Membranes ◽

The Status ◽

Direct Mass Spectrometry ◽

The Stability ◽

Blood Spot

The paper presents a method for rapid multi-omics investigation of biological samples using polypropylene bulk porous samplers. The use of porous samplers makes it easy to collect samples from the surface of the skin, mucous membranes, and biological fluids even in a field, and the surfaces of wounds and injuries. Collected samples do not require special storage conditions, and the samplers are lightweight and very compact. They can be used to monitor the condition of cosmonauts before, during, and after the spaceflight with the same sample collection method. The analysis of biomaterial applied to the sampler is performed using direct mass spectrometry methods, similar to the dried blood spot technique that is already used in clinical practice. However, bulk porous samplers allow expanding the range of analytes ionization conditions, which increases the stability and reliability of the ionization process, which expands the variety of analyzed molecules. The proposed method can be used to study compounds of various classes, including proteins, lipids, and metabolites, to systematically monitor the status of people in extreme conditions (athletes, astronauts), or to study the condition of patients in the clinic.

Download Full-text

Five-Year Stability Study of Free and Total Prostate-Specific Antigen Concentrations in Serum Specimens Collected and Stored at – 70°C or Less

The International Journal of Biological Markers ◽

10.1177/172460080702200308 ◽

2007 ◽

Vol 22 (3) ◽

pp. 206-213 ◽

Cited By ~ 1

Author(s):

D.A. Scaramuzzino ◽

K. Schulte ◽

B.N. Mack ◽

T.F. Soriano ◽

H.A. Fritsche

Keyword(s):

Prostate Specific Antigen ◽

Specific Antigen ◽

Stability Study ◽

Regression Analyses ◽

Altman Analysis ◽

Time Period ◽

Total Prostate Specific Antigen ◽

Deming Regression ◽

Lower Temperature ◽

The Stability

The stability of total (t) and free (f) prostate-specific antigen (PSA) in male serum specimens stored at –70°C or lower temperature for 4.7 to 4.9 years was studied. Until now, the stability of these analytes in serum has not been evaluated systematically beyond 2 years of storage at –70°C. Aliquots of frozen serum were thawed in 2001 and 2006 and assayed for tPSA and fPSA using a Dade Behring Dimension(r) RxL analyzer and reagents. tPSA values ranged from 0.07 to 69.94 and 0.00 to 69.83 ng/mL in 2001 and 2006, respectively, whereas fPSA values for the tested specimens ranged from 0.02 to 5.72 and 0.00 to 5.92, respectively. Deming regression analyses showed agreement in assay values over time as tPSA values yielded a slope of 1.0112 and a y-intercept of 0.0195; fPSA values produced a slope 1.0538 and a y-intercept of –0.0442; f/tPSA values yielded a slope of 0.9631 and a y-intercept of 0.1195. A Bland-Altman analysis of the data demonstrated analyte and ratio stability over this time period. We conclude that serum, when collected properly and stored at –70°C or lower temperature, may be used for tPSA and fPSA clinical studies for at least 5 years after collection.

Download Full-text

An Assessment of Mesoporous Silica Nanoparticle Architectures as Antigen Carriers

Pharmaceutics ◽

10.3390/pharmaceutics12030294 ◽

2020 ◽

Vol 12 (3) ◽

pp. 294 ◽

Cited By ~ 1

Author(s):

Xinyue Huang ◽

Helen E Townley

Keyword(s):

Mesoporous Silica ◽

Mesoporous Silica Nanoparticles ◽

High Surface ◽

Silica Nanoparticle ◽

Storage Conditions ◽

Surface Areas ◽

Antigen Complex ◽

The Stability ◽

And Storage ◽

Storage Temperatures

Mesoporous silica nanoparticles (MSNPs) have the potential to be used as antigen carriers due to their high surface areas and highly ordered pore network. We investigated the adsorption and desorption of diphtheria toxoid as a proof-of-concept. Two series of nanoparticles were prepared—(i) small pores (SP) (<10 nm) and (ii) large pores (LP) (>10 nm). SBA-15 was included as a comparison since this is commercially available and has been used in a large number of studies. External diameters of the particles ranged from 138 to 1509 nm, surface area from 632 to 1110 m2/g and pore size from 2.59 to 16.48 nm. Antigen loading was assessed at a number of different ratios of silica-to-antigen and at 4 °C, 20 °C and 37 °C. Our data showed that protein adsorption by the SP series was in general consistently lower than that shown by the large pore series. Unloading was then examined at 4 °C, 20 °C and 37 °C and a pH 1.2, 4.5, 6.8 and 7.4. There was a trend amongst the LP particles towards the smallest pores showing the lowest release of antigen. The stability of the MSNP: antigen complex was tested at two different storage temperatures, and storage in solution or after lyophilization. After 6 months there was negligible release from any of the particles under any of the storage conditions. The particles were also shown not to cause hemolysis.

Download Full-text