scholarly journals Selective adsorption of trypsin using molecularly imprinted polymers prepared with PEG-based hydrogels containing anionic functional monomers

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Takuya Kubo ◽  
Shunsuke Arimura ◽  
Toyohiro Naito ◽  
Koji Otsuka
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Basic Protein ◽  
Selective Adsorption ◽  
Complete Removal ◽  
Template Molecule ◽  
Functional Monomer ◽  
Nacl Solution ◽  
Protein Solution ◽  
Molecularly Imprinted ◽  
Functional Monomers

AbstractMolecularly imprinting (MI) hydrogels for selective adsorption of trypsin are reported. The trypsin imprinted hydrogels were prepared using a polyethylene glycol (PEG)-based dimethacrylate as a crosslinker and anionic functional monomers. The hydrogel prepared without any functional monomers showed significantly low ability to adsorb a variety of proteins. We optimized the concentration and the length of PEG units of the crosslinkers to achieve the complete removal of the template molecule and suitable selective adsorption. Additionally, the functional monomers chosen were anionic since the template, trypsin, is a basic protein. The adsorption tests for proteins, done on the prepared MI gels, indicated that the MI gel prepared with sodium allyl sulfonate (AS) as a functional monomer showed much higher selective adsorption for trypsin, even though a mixture of trypsin and cytochrome c was used as the protein solution. The selective adsorption was more effective in a NaCl solution in which the non-specific adsorption by a sulfonate is suppressed, similarly to our findings in a previous study. The MI gel prepared with acrylic acid also showed the selectivity, although the adsorption strength was lower than that of the MI gel containing AS. We believe that the present study constitutes the first approach for the selective adsorption of trypsin using PEG-based hydrogels.

The Effect on Adsorption Performance of Molecularly Imprinted Polymer Using the Different Functional Monomers

2011 ◽  
Vol 287-290 ◽  
pp. 334-337 ◽  
Author(s):  
Xiao Ying Yin ◽  
Xiang Hong Xu ◽  
Yi Fan Jiang ◽  
Yong Ming Luo ◽  
Ling Ying Luo
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Adsorption Properties ◽  
Template Molecule ◽  
Functional Monomer ◽  
Uv Spectrum ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Adsorption Performance ◽  
Equilibrium Binding ◽  
The One

To prepare molecularly imprinted polymers (MIPs) with good recognition properties，the effect on the MIPs adsorption performance were investigated from the different functional monomers. The MIPs were synthesized by using andrographolide as template, and α-methyl acrylic acid (MAA), acrylamide (AM) and 1 - vinylimidazole were used as functional monomer. Before polymerization, the interaction between the template molecule and the two functional monomers was studied by UV spectrum. After polymerization the different adsorption properties of MIPs prepared by the three types of functional monomers were compared by equilibrium binding. The results showed that the adsorption properties of the MIPs by 1-vinyl imidazole as functional monomer were similar to the MIPs by AM as functional, which the former is slightly higher than the latter and significantly higher than that of the one by MAA as functional monomer.

Water-compatible surface molecularly imprinted polymers with synergy of bi-functional monomers for enhanced selective adsorption of bisphenol A from aqueous solution

2016 ◽  
Vol 3 (1) ◽  
pp. 213-222 ◽  
Author(s):  
Feifei Duan ◽  
Chaoqiu Chen ◽  
Xiaofeng Zhao ◽  
Yongzhen Yang ◽  
Xuguang Liu ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Bisphenol A ◽  
Molecularly Imprinted Polymers ◽  
Selective Adsorption ◽  
Aqueous Media ◽  
Selective Removal ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Adsorption Performance

Water-compatible surface molecularly imprinted polymers were synthesized via bi-functional monomers and exhibited excellent adsorption performance for the selective removal of BPA from aqueous media.

scholarly journals Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Shanwen Zhao ◽  
Chanling Wei ◽  
Zhian Sun ◽  
Huachun Liu ◽  
Yanqiang Zhou ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Bovine Serum ◽  
Crosslinking Agent ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Restricted Access ◽  
Imprinted Polymers ◽  
Relative Standard ◽  
Restricted Access Media

Chloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. The CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. The adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. The CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with high-performance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7–1.5%. This material has broad application prospects in enrichment and separation.

Chiral Resolution of Racemic (±)-1,1'Bi (2-Naphthol) by Use of Molecularly Imprinted Polymers

2010 ◽  
Vol 150-151 ◽  
pp. 150-159
Author(s):  
Hong Xing Dong ◽  
Fei Tong ◽  
Jun Qing Li ◽  
Zhen Xing Wang ◽  
Yan Hui Wang ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
High Performance ◽  
Chiral Recognition ◽  
Polar Solvent ◽  
Stationary Phases ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Π Stacking Interaction

Polymers imprinted with (S)-(-)-1,1’-bi (2-naphthol) and (R)-(+)-1,1’-bi (2-naphthol) have been prepared by non-covalent imprinting. A combinational procedure was used to optimize the functional monomer and crosslinker. A copolymer of 2-vinylpyridine and divinylbenzene resulted in the best chiral recognition. The ratio of template to functional monomer and solvent in the pre-polymerization mixture were also optimized. The imprinted polymers were used as stationary phases in high-performance liquid chromatography (HPLC). The molecularly imprinted polymers (MIPs) were more selective when prepared using a less polar solvent. Effective separations of the enantiomers of racemic (±)-1, 1’-Bi (2-naphthol) were achieved by use of acetonitrile as mobile phase; no cross-selectivity was observed. Interactions between functional monomers and template were investigated by 1H NMR spectroscopy. The results suggest that hydrogen-bonding between the functional monomer and the template and π-π stacking interaction between the cross-linker and the template may contribute to chiral recognition.

Development of Improved Crosslinking Monomers for Molecularly Imprinted Materials

2002 ◽  
Vol 723 ◽  
Author(s):  
David A. Spivak ◽  
Martha Sibrian-Vazquez
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Polymer Complex ◽  
Functional Monomer ◽  
Design Synthesis ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Catalytic Sites ◽  
And Performance

AbstractMolecular imprinting involves the self-assembled complexation of a substrate to functional monomers to form a pre-polymer complex which is “locked-in” to place by copolymerization with an excess of crosslinking monomer. Removal of the template leaves binding or catalytic sites that are complementary in size, shape, and functionality to the template. Most of the research in molecularly imprinted materials has focused on choice of substrate or functional monomer of the pre-polymer complex. The cross-linking monomers have primarily been EGDMA or DVB, which are commercially available. Redirecting focus on the design of crosslinking monomers for molecular imprinting, we have developed new classes of crosslinked polymers to optimize the performance of molecularly imprinted polymers. The design of the new crosslinking monomers has followed two strategies: (1) development of new crosslinked materials for formation of the supporting matrix, and (2) development of crosslinking monomers that simultaneously serve as the functional monomer. The details of the design, synthesis, polymerization and performance of these new crosslinking monomers for molecularly imprinted polymers will be reported.

A Novel Method for Prepairing Higher Performance Picroside I Surface Molecularly Imprinted Polymers for TCM Research

2013 ◽  
Vol 785-786 ◽  
pp. 642-645
Author(s):  
Qing Shan Liu ◽  
Ke Qin Li ◽  
Jun Li ◽  
Xiao Ying Yin ◽  
Tian Hua Yan
Keyword(s):  
Electron Microscope ◽  
Molecularly Imprinted Polymers ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecular Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Scatchard Equation ◽  
Novel Method ◽  
Scanning Electron

To establish a novel method for preparing molecularly imprinted polymers for Picroside I with better performance on TCM research contrast to previous studies, we have prepared novel surface molecular imprinted polymers (S-MIPs) using Picroside I as the template molecule, Acrylamide (AM) as the functional monomer, and silica gel as the carrier. The morphology of S-MIPs was characterized by scanning electron microscope (SEM) and its static adsorption capacity was measured by the Scatchard equation.

Synthesis and evaluation of a molecularly imprinted polymer with high-efficiency recognition for dibutyl phthalate based on Mn-doped ZnS quantum dots

RSC Advances ◽  
2016 ◽  
Vol 6 (59) ◽  
pp. 54615-54622 ◽  
Author(s):  
Tao Li ◽  
Zhikun Gao ◽  
Ningwei Wang ◽  
Zhiping Zhou ◽  
Wanzhen Xu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Molecularly Imprinted Polymers ◽  
Dibutyl Phthalate ◽  
High Efficiency ◽  
Template Molecule ◽  
Functional Monomer ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Cross Linker ◽  
Mn Doped

Molecularly imprinted polymers with Mn-doped ZnS quantum dots were prepared using dibutyl phthalate as the template molecule, 3-aminopropyltriethoxysilane as the functional monomer and the tetraethoxysilane as the cross-linker.

scholarly journals Computer-Aided Design of Molecularly Imprinted Polymers for Simultaneous Detection of Clenbuterol and Its Metabolites

Polymers ◽  
2018 ◽  
Vol 11 (1) ◽  
pp. 17 ◽  
Author(s):  
Bingcheng Zhang ◽  
Xin Fan ◽  
Dayun Zhao
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Density Functional ◽  
Simultaneous Detection ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Dummy Template ◽  
Computer Aided

Molecular imprinting technology (MIT) offers an effective technique for efficient separation and enrichment of specific analytes from complicated matrices and has been used for illicit veterinary drug detectionin recent years due to its high selectivity, good chemical stability, and simple preparation. The development of in silico-based approaches has enabled the simulation of molecularly imprinted polymers (MIPs) to facilitate the selection of imprinting conditions such as template, functional monomer, and the best suitable solvent. In this work, using density functional theory (DFT), the molecularly imprinted polymers of clenbuterol and its metabolites were designed by computer-aided at B3LYP/6-31 + G (d, p) level. Screening molecular imprinting components such as functional monomers, cross-linkers, and solvents has been achieved in the computational simulation considerations. The simulation results showed that methacrylic acid (MAA) is the best functional monomer; the optimal imprinting ratio for both clenbuterol (CLB) and its dummy template molecule of phenylephrine (PE) to functional monomer is 1:3, while the optimal imprinting ratio for the two dummy template molecules of CLB’s metabolites is 1:5. Choosin gethyleneglycol dimethacrylate (EDGMA) as a crosslinker and aprotic solvents could increase the selectivity of the molecularly imprinted system. Atoms in Molecules (AIM) topology analysis was applied to investigate the template-monomer complexes bonding situation and helped to explain the nature of the reaction in the imprinting process. These theoretical predictions were also verified by the experimental results and found to be in good agreement with the computational results. The computer-simulated imprinting process compensates for the lack of clarity in the mechanism of the molecular imprinting process, and provides an important reference and direction for developing better recognition pattern towards CLB and its metabolite analytes in swine urine samples at the same time.

Study on Unbalanced Competitive Adsorption of Two Ginsenosides by Molecularly Imprinted Polymers

2019 ◽  
Vol 821 ◽  
pp. 144-152
Author(s):  
Wei Zhang ◽  
Yun Jie Ma ◽  
Qian Li ◽  
Shao Yan Wang
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Competitive Adsorption ◽  
High Specific Surface Area ◽  
Poly Vinyl Alcohol ◽  
Template Molecule ◽  
Recognition Mechanism ◽  
Ginsenoside Re ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers

In this study, a kind of homogeneous spherical molecularly imprinted polymers (MIPs) with high specific surface area was prepared, with ginsenoside Re (Re) as template molecule, methacrylic acid (MAA) as functional monomers, ethylglycol dimethacrylate (EGDMA) as crosslinker and poly (vinyl alcohol)-1788 (PVA-1788) as dispersant. The MIPs was characterized by field emission scanning electron microscopy (FESEM) and surface porosity detector, and competitive adsorption behavior of the MIPs was evaluated under different two-component adsorption conditions. The experimental results showed that MIPs had the regular mesopores structure, equilibrium adsorption isotherms of MIPs were in good agreement with Freundlich model under different adsorption conditions (single solute adsorption (SSA), simultaneously competitive adsorption (SCA) and preloaded competitive adsorption (PCA)). The concentration of template (Re) and competitors (Rg1) affect the results of competitive adsorption. Re has obvious priority in competitive adsorption, and this unbalanced competitive adsorption was the results of spatial selectivity and strong inter-molecule forces of MIPs toward Re. The analysis of competitive adsorption of molecularly imprinted materials is helpful to further study the recognition mechanism of molecularly imprinted adsorbent.

Sign in / Sign up

Export Citation Format

Share Document