Size-fractionated chlorophyll a and phycocyanin temporal variations in a highly eutrophic lake and its isolated karstic springs

2018 ◽  
Vol 47 (2) ◽  
pp. 118-127 ◽  
Author(s):  
Stephania L. Tsola ◽  
Christina Renta ◽  
Sofia C. Macingo ◽  
Hera Karayanni

Abstract Monthly variations of size-fractionated chlorophyll a and phycocyanin were studied in Lake Pamvotis between August 2016 and January 2017. Sampling was conducted at two sampling sites: in the main lake (Site 1: Lake) and in an adjacent man-made water ski lake with karstic springs (Site 2: Springs). Samples were fractionated into three size classes: 0.2–2 μm (pico), 2–20 μm (nano) and 20–180 μm (micro). According to chlorophyll a values, eutrophic to hypereutrophic conditions prevail at Site 1 and oligotrophic to mesotrophic conditions – at Site 2. Similarly, Site 1 was distinguished by higher concentration of phycocyanin compared to Site 2. Fractionated chlorophyll a showed monthly variations at Site 1 with alternations in the dominance between the two larger fractions. The maximum of the 0.2–2 μm fraction was observed in October but it contributed less to the total chlorophyll a content than nano- or microphytoplankton. Its contribution was higher at Site 2, reaching occasionally ~ 40% of the bulk chlorophyll a. However, nanophytoplankton was the fraction found to respond faster when disturbances occurred. At Site 1, phycocyanin correlated well with total chlorophyll a as well as with the micro- and nanophytoplankton fractions, indicating that cyanobacteria represent an important component of the large-sized phytoplankton in Lake Pamvotis.

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2699 ◽  
Author(s):  
Jian Li ◽  
Liqiao Tian ◽  
Qingjun Song ◽  
Zhaohua Sun ◽  
Hongjing Yu ◽  
...  

Monitoring of water quality changes in highly dynamic inland lakes is frequently impeded by insufficient spatial and temporal coverage, for both field surveys and remote sensing methods. To track short-term variations of chlorophyll fluorescence and chlorophyll-a concentrations in Poyang Lake, the largest freshwater lake in China, high-frequency, in-situ, measurements were collected from two fixed stations. The K-mean clustering method was also applied to identify clusters with similar spatio-temporal variations, using remote sensing Chl-a data products from the MERIS satellite, taken from 2003 to 2012. Four lake area classes were obtained with distinct spatio-temporal patterns, two of which were selected for in situ measurement. Distinct daily periodic variations were observed, with peaks at approximately 3:00 PM and troughs at night or early morning. Short-term variations of chlorophyll fluorescence and Chl-a levels were revealed, with a maximum intra-diurnal ratio of 5.1 and inter-diurnal ratio of 7.4, respectively. Using geostatistical analysis, the temporal range of chlorophyll fluorescence and corresponding Chl-a variations was determined to be 9.6 h, which indicates that there is a temporal discrepancy between Chl-a variations and the sampling frequency of current satellite missions. An analysis of the optimal sampling strategies demonstrated that the influence of the sampling time on the mean Chl-a concentrations observed was higher than 25%, and the uncertainty of any single Terra/MODIS or Aqua/MODIS observation was approximately 15%. Therefore, sampling twice a day is essential to resolve Chl-a variations with a bias level of 10% or less. The results highlight short-term variations of critical water quality parameters in freshwater, and they help identify specific design requirements for geostationary earth observation missions, so that they can better address the challenges of monitoring complex coastal and inland environments around the world.


2014 ◽  
Author(s):  
Y. Sakuno ◽  
K. Hatakeyama ◽  
Y. Miyamoto ◽  
A. Hatsuda ◽  
A. Mori ◽  
...  

Author(s):  
Megha Vishwakarma ◽  
P. S. Kulhare ◽  
G. S. Tagore

Field experiments were conducted during winter season of 2018-19 and 2019-20 with three sources of nutrient  viz., inorganic, organics (FYM, VC and biofertilizers) and their integration as main treatments and five levels [S1-0 (0-0-0 kg NPK ha-1), S2-100% (120-60-40 kg NPK ha-1), S3-150% (180-90-60 kg NPK ha-1), S4-200% (240-120-80 kg NPK ha-1) and S5-Soil Test based (STV) NPK i.e. 149-176-33 kg ha-1 in split plot design with three replications. The chlorophyll content (‘a’, ‘b’ and total) in leaves and Soil Plant Analyzer Development (SPAD) value were recorded at crown root initiation (CRI), tillering, jointing and milking stage of wheat. The pooled data of findings revealed that the treatment with inorganic sources showed significant increase in the SPAD readings (9.62, 15.54, 23.77 and 29.83), chlorophyll ‘a’ (0.76, 0.83, 1.47 and 0.63 mg g-1 leaf tissue), ‘b’ (0.44, 0.78, 0.87 and 0.57 mg g-1 leaf tissue) and total (1.19, 1.64, 2.25 and 1.14 mg g-1 leaf tissue) chlorophyll content in leaves over organic source at all the growth stages. All the levels of nutrient were significantly increased the chlorophyll content and SPAD value over control at all the stages except chlorophyll ‘a’ at jointing and milking stage. However, amongst the levels 150% and 200% NPK were found significantly superior to 100% NPK for SPAD value (8.32 and 8.71 at CRI and 12.56 and 12.19 at tillering), chlorophyll ‘a’ (0.73 and 0.70 mg g-1 leaf tissue at CRI), chlorophyll ‘b’ (0.46 and 0.45 mg g-1 leaf tissue at CRI, 0.68 and 0.71 mg g-1 leaf tissue at tillering and 0.53 and 0.59 mg g-1 leaf tissue at milking), respectively. The interaction results suggested that the 200% NPK with inorganic and integrated sources significantly superior to 100% NPK for chlorophyll ‘a’ content at jointing and milking stage. The application of 150% and 200% NPK with inorganic source were found significantly higher over the same level of NPK with integrated source of nutrient for total chlorophyll content and SPAD value at all the growth stages except 150% NPK for total chlorophyll at jointing and milking stage and SPAD value at milking stage. The correlation between SPAD value and chlorophyll ’a’, ‘b’, total were found significantly and positively at all growth stages. Coefficient of determination values between SPAD and chlorophyll content showed linear relationship at all the growth stages.


Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.


2016 ◽  
Vol 67 (7) ◽  
pp. 712 ◽  
Author(s):  
Shoaib Ur Rehman ◽  
Muhammad Bilal ◽  
Rashid Mehmood Rana ◽  
Muhammad Naveed Tahir ◽  
Muhammad Kausar Nawaz Shah ◽  
...  

Heat and drought are among the major obstacles confronting crop production under climate change. The present study was conducted to evaluate 50 diverse wheat genotypes for cell membrane stability (CMS) and chlorophyll content at seedling and anthesis stages under heat and drought stress conditions, to understand the effect of the two abiotic factors and to find promising genotypes for future breeding. Experiments were conducted in the glasshouse (seedling stage) and the field (anthesis stage). Analysis of variance showed significant variation (P ≤ 0.05) for all of the traits at seedling and anthesis stages. High levels of broad-sense heritability and genetic advance at 5% selection intensity indicated the presence of a high genetic component of variation and potential for genetic improvement through selection among the existing genetic variation. CMS showed a significant positive correlation with 1000-grain weight (TGW) under heat and drought conditions at both seedling and anthesis stages. Chlorophyll a/b ratio at seedling stage exhibited a significant negative correlation (r = –0.39, P < 0.05) with TGW under heat stress. Total chlorophyll content was significantly (r = 0.42, P < 0.05) correlated with TGW under heat stress at anthesis. Genotypes ETAD248 and ETAD7 showed the highest CMS and TGW values, whereas their chlorophyll a/b values were lowest, at both seedling and anthesis stages under heat and drought stress conditions. Higher CMS and total chlorophyll content, and lower chlorophyll a/b, were found to be useful indicators to identify genotypes with high TGW under heat and drought stress conditions. This study indicated the possibility of using seedling resistance as an indicator for later stage response in breeding for heat and drought resistance. The resistant genotypes identified can be used as potential germplasm in breeding programs.


2014 ◽  
Vol 14 (4) ◽  
pp. 601-608
Author(s):  
D.-W. Kim ◽  
J.-H. Min ◽  
M. Yoo ◽  
M. Kang ◽  
K. Kim

The primary goal of this study is to shed light on some important factors that control algal bloom in a large-scale regulated river system. Long-term impacts of environmental conditions on algal dynamics were investigated in the Paldang dam watershed, Korea. Dam inflow, water temperature, chlorophyll-a, TN, PO4-P and TP data collected at five major dams located on the North Han River (NHR) and at four water quality monitoring sites on the South Han River were analyzed for 21 years (1992 to 2012) to examine spatio-temporal variations in each. A pattern of slightly increasing chlorophyll-a and nutrient levels in the NHR since 2001 indicates that algal dynamics were affected by the increased nutrient levels as well as the reduced flow conditions (−10% to −37%). The temporal variations in monthly averaged data collected during summer monsoon seasons (mainly July) over the two decades show that high chlorophyll-a levels observed in both rivers corresponded to the relatively lower flow condition, which means a reduced amount of dam water release due to low or no rainfall over a short period of time, and abnormally high water temperature. This study shows that flow control is most critical for effectively managing algal level in the rivers in the short term, and nutrient management in the watershed is the key to reducing the potential for algal bloom in the long term.


2020 ◽  
Author(s):  
aiping huang

&lt;p&gt;Lake eutrophication is a key point in water environmental problems in the world. Spatiotemporal variations of nutrients and chlorophyll-a and eutrophication index in Poyang Lake, the largest freshwater lake in China, are analyzed in this paper basing on field observation data at 17 sampling points from 2011 to 2016. The results show that nutrient concentrations have obvious seasonality characteristics and present bigger values in the low water period than the high water period. The peak value of chlorophyll-a concentration appears in July and October respectively. As a whole, the eutrophication index in the low water period is higher than the high water period, and the maximum value is found in October which mainly due to the high chlorophyll-a concentration. Poyang Lake is at light eutrophication level from 2011 to 2012, and mesotrophic from 2013 to 2016. From the perspective of space, nutrient concentrations in the southern part of the lake is higher than the northern part in general, and chlorophyll-a and eutrophication index show the similar law. This paper makes a quantitative analysis for spatial and temporal variations of eutrophication which benefit the water management especially water pollution control in Poyang Lake&lt;/p&gt;


2014 ◽  
Vol 26 (2) ◽  
pp. 176-185 ◽  
Author(s):  
Edélti Faria Albertoni ◽  
Cleber Palma-Silva ◽  
Claudio Rossano Trindade Trindade ◽  
Leonardo Marques Furlanetto

AIM: The main objective of this work is to describe the changes in water characteristics of a shallow subtropical lake, in periods with and without growing of macrophytes, related to periods of clear-macrophyte dominance and turbid-phytoplankton dominance states. METHODS: The study was conducted in Biguás Lake, in the south coastal plain of Brazil (32° 04' 43" S and 52° 10' 03" W). Samplings were carried out monthly between October 2000 and November 2013. The limnological variables measured in the water column were dissolved oxygen (DO), water temperature, pH, electrical conductivity (EC), chlorophyll-a, total nitrogen (TN), total phosphorous (TP) and suspended material (SM). Data were grouped according to periods with macrophyte growth dominance (MD) and without macrophytes, with phytoplankton dominance (PD), and applied t- tests among TP, TN, Chlorophyll-a and SM. During macrophyte growth we estimated the coverage (%) and biomass variation of plants. RESULTS: Over the 13 years, the lake was well oxygenated, alkaline, and with a temperature variation according to subtropical seasonality. The lower values of all of the limnological variables were verified during periods of macrophyte growth, characterizing periods of clear and turbid waters. CONCLUSIONS: The influence of aquatic macrophytes in improving water quality in this shallow lake during the studied period, reducing nutrient concentrations, chlorophyll-a and suspended material in water, favoring the maintenance of a clear water state, was verified.


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