scholarly journals Short Note: Internal Size Standard for Microsatellite Genotyping

2009 ◽  
Vol 58 (1-6) ◽  
pp. 94-96
Author(s):  
Chin Hong Ng ◽  
S. L. Lee ◽  
K. K. S. Ng
Keyword(s):  
Fragment Size ◽  
Short Note ◽  
Microsatellite Genotyping ◽  
Taq Dna Polymerase ◽  
Nucleotide Addition ◽  
Size Standard ◽  
The Mean ◽  
The Cost ◽  
House Production ◽  
Commercial Size

AbstractThe in-house production of fluorescently labelledinternal size standard offers the advantage of cost savingover the commercial size standard in microsatellitegenotyping. Based on the reported in-house internal sizestandard protocol, we have improved the method by generating21 DNA fragments (in a standard named as HM-400) with each size similar to that of the commercialsize standard. The consistent amplification of the correctfragment size was optimised via primer modulationfor non-templated nucleotide addition by Taq DNA polymerase.A total of six microsatellite loci were used toassess the accuracy of HM-400 and the mean standarddeviation of the size data was 0.19. The differencesbetween the fragment size means for samples sizedusing HM-400 and commercial size standard were smallwith an average of 0.29 bp. The production cost of HM-400 was only 10% of the cost of commercial size standard.

Injuries to the Coronoid Process of the Ulna with Involvement of the Lesser Sigmoid Notch

2020 ◽  
Author(s):  
Valentin Rausch ◽  
Sina Neugebauer ◽  
Tim Leschinger ◽  
Lars Müller ◽  
Kilian Wegmann ◽  
...  
Keyword(s):  
Fragment Size ◽  
Coronoid Process ◽  
Annular Ligament ◽  
Concomitant Injuries ◽  
Proximal Ulna ◽  
Sigmoid Notch ◽  
Coronoid Fractures ◽  
The Mean ◽  
The Stability ◽  
Arthritic Changes

Abstract Introduction This study aimed to describe the involvement of the lesser sigmoid notch in fractures to the coronoid process. We hypothesized that injuries to the lateral aspect of the coronoid process regularly involve the annular ligament insertion at the anterior lesser sigmoid notch. Material and Methods Patients treated for a coronoid process fracture at our institution between 06/2011 and 07/2018 were included. We excluded patients < 18 years, patients with arthritic changes or previous operative treatment to the elbow, and patients with concomitant injuries to the proximal ulna. In patients with involvement of the lesser sigmoid notch, the coronoid height and fragment size (anteroposterior, mediolateral, and craniocaudal) were measured. Results Seventy-two patients (mean age: 47 years ± 17.6) could be included in the study. Twenty-one patients (29.2%) had a fracture involving the lateral sigmoid notch. The mean anteroposterior fragment length was 7 ± 1.6 mm. The fragment affected a mean of 43 ± 10.8% of the coronoid height. The mean mediolateral size of the fragment was 10 ± 5.0 mm, and the mean cranio-caudal size was 7 ± 2.7 mm. Conclusion Coronoid fractures regularly include the lesser sigmoid notch. These injuries possibly affect the anterior annular ligament insertion which is important for the stability of the proximal radioulnar joint and varus stability of the elbow.

Design and development of manually operated gladiolus planter

2018 ◽  
Vol 5 (01) ◽  
Author(s):  
TAPAN K. KHURA ◽  
H. L. KUSHWAHA ◽  
SATISH D LANDE ◽  
PKSAHOO . ◽  
INDRA L . KUSHWAHA
Keyword(s):  
Coefficient Of Variation ◽  
Field Capacity ◽  
Power Source ◽  
Forward Speed ◽  
Cereal Crop ◽  
Maximum Coefficient ◽  
Coefficient Of Uniformity ◽  
The Mean ◽  
The Cost ◽  
Immense Potential

Floriculture is an age-old farming activity in India having immense potential for generating selfemployment and income to farmers. However, the cost of cultivation of flower is high as compared to cereal crop. Level of mechanization for different field operations is one but foremost reason for the higher cost of cultivation. As most of the Indian farmers are marginal and small, a need for manually operated gladiolus planter was felt. The geometric properties of gladiolus corm were determined for designing the seed metering system and seed hopper of the planter. The planter was evaluated in the field when pulled by two persons as a power source and guided by a person. The coefficient of variation and highest deviation from the mean spacing was observed as 12.93% and 2.65cm respectively. The maximum coefficient of uniformity of 90.59% was observed for a nominal corm spacing of 15cm at 0.56 kmh-1 forward speed. An average MISS percentage was observed as 2.65 and 2.25 for nominal corm spacing of 15 and 20 cm. The multiple index was zero for two levels corm spacing and forward speed of operation. The QFI was found in the range of 97.2 and 97.9 percent. The average field capacity of the planter was observed as 0.02 hah-1.The average draft requirement of the planter was found as 821 ± 50.3 N.

scholarly journals Seasonal Influenza and Low Flu Vaccination Coverage as Important Factors Modifying the Costs and Availability of Hospital Services in Poland: A Retrospective Comparative Study

2021 ◽  
Vol 18 (10) ◽  
pp. 5173
Author(s):  
Robert Susło ◽  
Piotr Pobrotyn ◽  
Lidia Brydak ◽  
Łukasz Rypicz ◽  
Urszula Grata-Borkowska ◽  
...  
Keyword(s):  
Influenza Season ◽  
Influenza Infection ◽  
Hospital Staff ◽  
University Hospital ◽  
Control Group ◽  
Admission Rates ◽  
Risk Of Death ◽  
The Mean ◽  
Retrospective Comparative Study ◽  
The Cost

Introduction: Influenza infection is associated with potential serious complications, increased hospitalization rates, and a higher risk of death. Materials and Methods: A retrospective comparative analysis of selected indicators of hospitalization from the University Hospital in Wroclaw, Poland, was carried out on patients with confirmed influenza infection in comparison to a control group randomly selected from among all other patients hospitalized on the respective wards during the 2018–2019 influenza season. Results: The mean laboratory testing costs for the entire hospital were 3.74-fold higher and the mean imaging test costs were 4.02-fold higher for patients with confirmed influenza than for the control group; the hospital expenses were additionally raised by the cost of antiviral therapy, which is striking when compared against the cost of a single flu vaccine. During the 2018–2019 influenza season, influenza infections among the hospital patients temporarily limited the healthcare service availability in the institution, which resulted in reduced admission rates to the departments related to internal medicine; the mean absence among the hospital staff totaled approximately 7 h per employee, despite 7.3% of the staff having been vaccinated against influenza at the hospital’s expense. Conclusions: There were significant differences in the hospitalization indicators between the patients with confirmed influenza and the control group, which markedly increased the hospital care costs in this multi-specialty university hospital.

The cost of a general stochastic epidemic

1972 ◽  
Vol 9 (02) ◽  
pp. 257-269 ◽  
Author(s):  
J. Gani ◽  
D. Jerwood
Keyword(s):  
Recursive Equation ◽  
Stieltjes Transform ◽  
Stochastic Epidemic ◽  
The Mean ◽  
The Cost ◽  
Mean Cost ◽  
General Stochastic

This paper is concerned with the cost Cis = aWis + bTis (a, b &gt; 0) of a general stochastic epidemic starting with i infectives and s susceptibles; Tis denotes the duration of the epidemic, and Wis the area under the infective curve. The joint Laplace-Stieltjes transform of (Wis, Tis ) is studied, and a recursive equation derived for it. The duration Tis and its mean Nis are considered in some detail, as are also Wis and its mean Mis . Using the results obtained, bounds are found for the mean cost of the epidemic.

scholarly journals Comparison of the costs of HPV testing through community health campaigns versus home-based testing in rural Western Kenya: a microcosting study

BMJ Open ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. e033979
Author(s):  
Easter Elizabeth Olwanda ◽  
James G Kahn ◽  
Yujung Choi ◽  
Jessica Yasmine Islam ◽  
Megan Huchko
Keyword(s):  
Community Health ◽  
Outcome Measures ◽  
Health Campaigns ◽  
Capital Goods ◽  
Western Kenya ◽  
Home Based ◽  
Registration Number ◽  
The Mean ◽  
The Cost ◽  
Mean Cost

ObjectivesTo estimate the cost of human papillomavirus (HPV)-based screening through community health campaigns (CHCs) and home-based testing.SettingCHCs and home-based testing in six communities in rural Western Kenya.ParticipantsCHCs and home-based screening reached 2297 and 1002 women aged 25–65 years, respectively.Outcome measuresOutcome measures were overall cost per woman screened achieved through the CHCs and home-based testing and the cost per woman for each activity comprising the screening intervention.ResultsThe mean cost per woman screened through CHCs and home-based testing were similar, at $37.7 (range $26.4–$52.0) and $37.1 (range $27.6–$54.0), respectively. For CHCs, personnel represented 49% of overall cost, supplies 25%, services 5% and capital goods 23%. For home-based testing, these were: personnel 73%, supplies 25%, services 1% and capital goods 2%. A greater number of participants was associated with a lower cost per participant.ConclusionsThe mean cost per woman screened is comparable for CHC and home-based testing, with differences in type of input. The CHCs generally reached more eligible women in the six communities, whereas home-based strategies more efficiently reached populations with low screening rates.Trial registration numberNCT02124252.

Optimal stationary strategies in leavable Markov decision processes

1990 ◽  
Vol 27 (01) ◽  
pp. 134-145
Author(s):  
Matthias Fassbender
Keyword(s):  
Transition Probabilities ◽  
Practical Applications ◽  
Stationary Strategies ◽  
Countable State ◽  
Markov Decision ◽  
Bias Optimality ◽  
The Mean ◽  
Optimal Stationary Strategies ◽  
The Cost ◽  
Reward Criterion

This paper establishes the existence of an optimal stationary strategy in a leavable Markov decision process with countable state space and undiscounted total reward criterion. Besides assumptions of boundedness and continuity, an assumption is imposed on the model which demands the continuity of the mean recurrence times on a subset of the stationary strategies, the so-called ‘good strategies'. For practical applications it is important that this assumption is implied by an assumption about the cost structure and the transition probabilities. In the last part we point out that our results in general cannot be deduced from related works on bias-optimality by Dekker and Hordijk, Wijngaard or Mann.

scholarly journals Addition of Flucytosine to Fluconazole for the Treatment of Cryptococcal Meningitis in Africa: A Multicountry Cost-effectiveness Analysis

2019 ◽  
Vol 70 (1) ◽  
pp. 26-29 ◽  
Author(s):  
Tinevimbo Shiri ◽  
Angela Loyse ◽  
Lawrence Mwenge ◽  
Tao Chen ◽  
Shabir Lakhi ◽  
...  
Keyword(s):  
Sensitivity Analysis ◽  
Cost Effectiveness ◽  
Cryptococcal Meningitis ◽  
Cost Effective ◽  
Immunodeficiency Virus ◽  
The Mean ◽  
The Cost ◽  
The Impact ◽  
Costing Analysis ◽  
Very High

Abstract Background Mortality from cryptococcal meningitis remains very high in Africa. In the Advancing Cryptococcal Meningitis Treatment for Africa (ACTA) trial, 2 weeks of fluconazole (FLU) plus flucytosine (5FC) was as effective and less costly than 2 weeks of amphotericin-based regimens. However, many African settings treat with FLU monotherapy, and the cost-effectiveness of adding 5FC to FLU is uncertain. Methods The effectiveness and costs of FLU+5FC were taken from ACTA, which included a costing analysis at the Zambian site. The effectiveness of FLU was derived from cohorts of consecutively enrolled patients, managed in respects other than drug therapy, as were participants in ACTA. FLU costs were derived from costs of FLU+5FC in ACTA, by subtracting 5FC drug and monitoring costs. The cost-effectiveness of FLU+5FC vs FLU alone was measured as the incremental cost-effectiveness ratio (ICER). A probabilistic sensitivity analysis assessed uncertainties and a bivariate deterministic sensitivity analysis examined the impact of varying mortality and 5FC drug costs on the ICER. Results The mean costs per patient were US $847 (95% confidence interval [CI] $776–927) for FLU+5FC, and US $628 (95% CI $557–709) for FLU. The 10-week mortality rate was 35.1% (95% CI 28.9–41.7%) with FLU+5FC and 53.8% (95% CI 43.1–64.1%) with FLU. At the current 5FC price of US $1.30 per 500 mg tablet, the ICER of 5FC+FLU versus FLU alone was US $65 (95% CI $28–208) per life-year saved. Reducing the 5FC cost to between US $0.80 and US $0.40 per 500 mg resulted in an ICER between US $44 and US $28 per life-year saved. Conclusions The addition of 5FC to FLU is cost-effective for cryptococcal meningitis treatment in Africa and, if made available widely, could substantially reduce mortality rates among human immunodeficiency virus–infected persons in Africa.

scholarly journals Energy cost and muscular activity required for propulsion during walking

2003 ◽  
Vol 94 (5) ◽  
pp. 1766-1772 ◽  
Author(s):  
Jinger S. Gottschall ◽  
Rodger Kram
Keyword(s):  
Body Weight ◽  
Muscular Activity ◽  
Metabolic Cost ◽  
Medial Gastrocnemius ◽  
Emg Activity ◽  
Horizontal Force ◽  
Normal Walking ◽  
The Mean ◽  
The Cost ◽  
Muscle Actions

We reasoned that with an optimal aiding horizontal force, the reduction in metabolic rate would reflect the cost of generating propulsive forces during normal walking. Furthermore, the reductions in ankle extensor electromyographic (EMG) activity would indicate the propulsive muscle actions. We applied horizontal forces at the waist, ranging from 15% body weight aiding to 15% body weight impeding, while subjects walked at 1.25 m/s. With an aiding horizontal force of 10% body weight, 1) the net metabolic cost of walking decreased to a minimum of 53% of normal walking, 2) the mean EMG of the medial gastrocnemius (MG) during the propulsive phase decreased to 59% of the normal walking magnitude, and yet 3) the mean EMG of the soleus (Sol) did not decrease significantly. Our data indicate that generating horizontal propulsive forces constitutes nearly half of the metabolic cost of normal walking. Additionally, it appears that the MG plays an important role in forward propulsion, whereas the Sol does not.

The Race for Online Reputation: Implications for Platforms, Firms, and Consumers

2021 ◽  
Author(s):  
Mingwen Yang ◽  
Zhiqiang (Eric) Zheng ◽  
Vijay Mookerjee
Keyword(s):  
Adverse Event ◽  
Marketing Mix ◽  
Digital Economy ◽  
The Other ◽  
The Mean ◽  
The Absolute ◽  
The Cost ◽  
Online Reputation ◽  
Better Than

Online reputation has become a key marketing-mix variable in the digital economy. Our study helps managers decide on the effort they should use to manage online reputation. We consider an online reputation race in which it is important not just to manage the absolute reputation, but also the relative rating. That is, to stay ahead, a firm should try to have ratings that are better than those of its competitors. Our findings are particularly significant for platform owners (such as Expedia or Yelp) to strategically grow their base of participating firms: growing the middle of the market (firms with average ratings) is the best option considering the goals of the platform and the other stakeholders, namely incumbents and consumers. For firms, we find that they should increase their effort when the mean market rating increases. Another key insight for firms is that, sometimes, adversity can come disguised as an opportunity. When an adverse event strikes the industry (such as a reduction in sales margin or an increase in the cost of effort), a firm’s profit can increase if it can manage this event better than its competitors.

scholarly journals Direct cost of connecting, maintaining and disconnecting patient-controlled analgesia pump

2014 ◽  
Vol 48 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Áquila Lopes Gouvêa ◽  
Antônio Fernandes Costa Lima
Keyword(s):  
Quantitative Research ◽  
Unit Cost ◽  
Patient Controlled Analgesia ◽  
Unified Health System ◽  
Effective Decision ◽  
The Mean ◽  
Effective Decision Making ◽  
Cost Of Materials ◽  
The Cost ◽  
Probabilistic Sample

Quantitative research that aimed to identify the mean total cost (MTC) of connecting, maintaining and disconnecting patient-controlled analgesia pump (PCA) in the management of pain. The non-probabilistic sample corresponded to the observation of 81 procedures in 17 units of the Central Institute of the Clinics Hospital, Faculty of Medicine, University of Sao Paulo. We calculated the MTC multiplying by the time spent by nurses at a unit cost of direct labor, adding the cost of materials and medications/solutions. The MTC of connecting was R$ 107.91; maintenance R$ 110.55 and disconnecting R$ 4.94. The results found will subsidize discussions about the need to transfer money from the Unified Health System to hospitals units that perform this technique of analgesic therapy and it will contribute to the cost management aimed at making efficient and effective decision-making in the allocation of available resources.

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