Evaluation the vigour of urban green lawn grown under long-term shade conditions by the use of chlorophyll fluorescence technique

Abstract Unfavorable light conditions in urban areas are one of the most important cause of inappropriate grass communities condition. The possibility to detect the plant stress caused by shade is an important element in shaping the environment. The answer to following questions: what is the ability to detect the stress caused by shade in chosen lawn varieties of Perennial ryegrass by using the chlorophyll a fluorescence (O-J-I-P test) and which of tested varieties has the best properties to create grasslands in reduced light conditions is the aim of this work. Two-factor experimental micro-plot was conducted with three varieties and three different shadowing variants. Chlorophyll a fluorescence measurements were provided and were compared to leaf density. Our results explored significant difference between selected varieties in the terms of their photosynthetic apparatus adaption to light conditions. During May, all tested varieties were characterized by the rise of all fluorescence curve points under lower light intensity. The largest changes under shade conditions were noticed for the variety ‘Taya’. During next months a declining trend of photosynthetic efficiency for this variety was observed. On the basis of our results, we assume that each variety has unique threshold and needs of light intensity.

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Chlorophyll a fluorescence as an indicator of water stress in Calophyllum brasiliense

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha48111757 ◽

2020 ◽

Vol 48 (1) ◽

pp. 210-220 ◽

Cited By ~ 2

Author(s):

Lucas C. REIS ◽

Silvana P.Q. SCALON ◽

Daiane DRESCH ◽

Andressa Caroline FORESTI ◽

Cleberton C. SANTOS ◽

...

Keyword(s):

Water Deficit ◽

Chlorophyll A ◽

Chlorophyll A Fluorescence ◽

Stress Condition ◽

Water Status ◽

Photosynthetic Apparatus ◽

Stress Indicator ◽

Fluorescence Measurements ◽

Calophyllum Brasiliense ◽

And Function

The objective of this study was to evaluate chlorophyll a fluorescence as a stress indicator in Calophyllum brasiliense Cambess seedlings grown with different concentrations of abscisic acid (ABA) under intermittent water deficit condition: daily irrigation without ABA (I); daily irrigation + 10 μM ABA (I 10); daily irrigation + 100 μM ABA (I 100); suspension of daily irrigation without ABA (SI); suspension of daily irrigation + 10 μM ABA (SI 10) and suspension of daily irrigation + 100 μM ABA (SI 100). The intermittent water deficit reduces water status and impairs the photochemical apparatus functioning and seedling quality. The fluorescence measurements helped identify the stress condition of water deficit in the cultivation of C. brasiliense and the beneficial effect of the application of 10 μM of ABA in minimizing stress and facilitating the recovery of seedlings after re-irrigation, while maintaining the integrity and function of the photosynthetic apparatus.

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DAYTIME VARIATIONS OF CHLOROPHYLL A FLUORESCENCE IN PAU D'ALHO SEEDLINGS

Revista Árvore ◽

10.1590/0100-67622016000600007 ◽

2016 ◽

Vol 40 (6) ◽

pp. 1023-1030 ◽

Cited By ~ 2

Author(s):

Marcos Antonio Bacarin ◽

Emanuela Garbin Martinazzo ◽

Daniela Cassol ◽

Antelmo Ralph Falqueto ◽

Diolina Moura Silva

Keyword(s):

Chlorophyll A ◽

Chlorophyll A Fluorescence ◽

Photochemical Efficiency ◽

Photosynthetic Apparatus ◽

Photosynthetic Active Radiation ◽

High Radiation ◽

Active Radiation ◽

Fluorescence Measurements ◽

Subsequent Decrease ◽

Modulated Fluorescence

ABSTRACT Analysis of transient and modulated fluorescence of chlorophyll a were made at one-hour intervals during an eight-hour period starting at 07:30h aiming to study mechanisms of photoprotection against high radiation and temperature in Gallesia integrifolia plants. Seeds were germinated inside plastic pots containing soil as substrate. At 120 days after emergence, chlorophyll fluorescence measurements were performed using Handy-PEA and FMS2 fluorometers. During the course of a day, an increase and a subsequent decrease in temperature and in photosynthetic active radiation were observed until 12:30h. Changes in transient kinetic curves of chlorophyll a fluorescence were identified. This resulted in changes in JIP test parameters. An increase during the period of high radiation and temperature may be stressed in relation to variables related to dissipation flux and appearance of positive -K and -L bands. Considering the modulated fluorescence, high values of non-photochemical quenching coefficients associated with lower values of effective photochemical efficiency of the photosystem II (FV'/ FM') and current photochemical efficiency of PSII (φPSII) could be observed during early morning. This was probably the result of an inhibition of the biochemical phase of photosynthesis. It can be concluded that Gallesia integrifolia decrease its photochemical activity with the increase in the photosynthetic active radiation, demonstrating a photoinhibitory effect under high irradiance conditions, but without irreversible damages to the photosynthetic apparatus.

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Photosynthetic Efficiency and Anatomical Structure of Pepper Leaf (Capsicum annuum L.) Transplants Grown under High-Pressure Sodium (HPS) and Light-Emitting Diode (LED) Supplementary Lighting Systems

Plants ◽

10.3390/plants10101975 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1975

Author(s):

Anna Sobczak ◽

Marzena Sujkowska-Rybkowska ◽

Janina Gajc-Wolska ◽

Waldemar Kowalczyk ◽

Wojciech Borucki ◽

...

Keyword(s):

High Pressure ◽

Chlorophyll A ◽

Chlorophyll A Fluorescence ◽

Light Emitting Diodes ◽

Photochemical Efficiency ◽

Photosynthetic Apparatus ◽

Light Emitting ◽

Fluorescence Measurements ◽

Supplemental Lighting ◽

Lighting Systems

The aim of this study was to evaluate the effects of various supplemental greenhouse lighting systems, i.e., high-pressure sodium lamps and mixtures of red and blue light-emitting diodes, on the photochemical efficiency, anatomical leaf structure, and growth of the two pepper cultivars. The intensity levels of the photosynthetically active radiation were the same for both light treatments. In this study, the relative chlorophyll content was measured. Additionally, certain parameters of chlorophyll a fluorescence were measured under ambient light or after dark adaptation. The obtained results showed that the application of light-emitting diodes (LEDs) as supplemental lighting positively affected the anatomical leaf characteristics and plant growth. The leaves of both pepper cultivars were thicker and had larger palisade parenchyma cells under LED supplemental lighting compared to leaves grown under high-pressure sodium (HPS) lamps. Moreover, the mesophyll cells of seedlings grown under LEDs contained more chloroplasts than those growing under HPS lighting. The chlorophyll a fluorescence measurements of pepper seedlings grown under LEDs showed significant increases in photosynthetic apparatus performance index (PI) values compared to plants grown under HPS lamps; however, the values for this index were higher in cv. ‘Aifos’ as compared to cv. ‘Palermo’. We recommend that supplemental lighting systems are applied with caution, as their performance appears to depend not only on the light spectrum but also on the cultivar.

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Multiple effects of chromate on the photosynthetic apparatus of Spirodela polyrhiza as probed by OJIP chlorophyll a fluorescence measurements

Environmental Pollution ◽

10.1016/s0269-7491(01)00091-4 ◽

2001 ◽

Vol 115 (1) ◽

pp. 49-64 ◽

Cited By ~ 222

Author(s):

K.-J Appenroth ◽

J Stöckel ◽

A Srivastava ◽

R.J Strasser

Keyword(s):

Chlorophyll A ◽

Chlorophyll A Fluorescence ◽

Photosynthetic Apparatus ◽

Spirodela Polyrhiza ◽

Fluorescence Measurements

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Chlorophyll a Fluorescence Transient and 2-Dimensional Electrophoresis Analyses Reveal Response Characteristics of Photosynthesis to Heat Stress in Malus. ‘Prairifire’

Plants ◽

10.3390/plants9081040 ◽

2020 ◽

Vol 9 (8) ◽

pp. 1040

Author(s):

Tao Wang ◽

Siqian Luo ◽

Yingli Ma ◽

Lingyu Li ◽

Yinfeng Xie ◽

...

Keyword(s):

Heat Stress ◽

Heat Shock ◽

Chlorophyll A ◽

Chlorophyll A Fluorescence ◽

Photosynthetic Apparatus ◽

Protective Mechanism ◽

Response Characteristics ◽

Fluorescence Transient ◽

Chlorophyll A Fluorescence Transient ◽

Dimensional Electrophoresis

Flowering crabapples are a series of precious ornamental woody plants. However, their growth and development are inhibited in the subtropical regions due to the weak photosynthesis under high-temperature environment in the summer. Chlorophyll a fluorescence transient and 2-dimensional electrophoresis (2-DE) analyses were conducted to investigate the response characteristics of photosynthesis under simulated 38 °C heat stress in leaves of Malus. ‘Prairifire’, a spring-red leaf cultivar of flowering crabapple with strong thermal adaptability. In the present study, the net photosynthetic rate (Pn) was significantly decreased during the heat shock process, which showed a similar trend to the stomatal conductance (Gs), indicating a sensitive stomatal behavior to heat stress. Moreover, an efficient reaction center in photosystem II (PSII), and a functionally intact oxygen-evolving complex (OEC) conferred strong photosynthetic adaptability under heat stress. The higher level of transketolase (TK) under 48-h heat shock treatment was considered a protective mechanism of photosynthetic apparatus. However, heat stress inhibited the functions of light harvesting complex II (LHCII), electron transport in PSII, and the levels of key enzymes in the Calvin cycle, which were considered as the reasons causing an increase in the proportion of non-stomatal restrictions.

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