Effects of intraplantar administration of Complete Freund’s Adjuvant (CFA) on rotarod performance in mice

2019 ◽  
Vol 19 (4) ◽  
pp. 805-811
Author(s):  
Ahmad Altarifi ◽  
Mohammad Alsalem ◽  
Ayman Mustafa
Keyword(s):  
Mechanical Allodynia ◽  
Complete Freund’S Adjuvant ◽  
Control Group ◽  
Pain Mechanisms ◽  
Rotarod Performance ◽  
Inflammatory Nociception ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Von Frey Filaments

Abstract Background and aims Preclinical animal models are crucial to study pain mechanisms and assess antinociceptive effects of medications. One major problem with current animal behavioral models is their lack of face validity with human nociception and the vulnerability for false-positive results. Here, we evaluated the usefulness of rotarod as a new way to assess inflammatory nociception in rodents. Methods Adult male mice were injected with saline or Complete Freund’s Adjuvant (CFA) in the left hindpaws. Mechanical allodynia and rotarod performance were evaluated before and after the administration of CFA. Mechanical allodynia was measured using von Frey filaments. Long-term effect of CFA on rotarod performance was also assessed for 2 weeks. Results Our results showed that CFA administration decreased pain threshold and increased sensitivity to von Frey filaments compared to control group. In rotarod experiments, the starting speed of the rod rotation started at four RPM, and accelerated until it reached 40 RPM in 5 min. Rotarod performance was enhanced from day to day in the control group. However, rotarod performance in CFA group was attenuated after CFA administration, which was significant after 24 h compared to vehicle. This attenuation was blocked by ibuprofen. Haloperidol administration (positive control) produced similar results to CFA administration. CFA did not produce significant attenuation of rotarod performance after 1 week post-injection. Conclusions Collectively, our findings could encourage the use of rotarod assay to measure acute (but not chronic) inflammatory nociception as a useful tool in rodents.

scholarly journals ERDOSTEINE: AN EFFECTIVE ANTIOXIDANT FOR PROTECTING COMPLETE FREUND’S ADJUVANT INDUCED ARTHRITIS IN RATS

2021 ◽  
pp. 71-75
Author(s):  
BANYLLA SYNMON ◽  
SANHATIDUTTA ROY ◽  
SUTAPA BISWAS MAJEE ◽  
MEGHNA PAUL ◽  
SANDIPAN DASGUPTA
Keyword(s):  
Body Weight ◽  
Test Group ◽  
The Body ◽  
Complete Freund’S Adjuvant ◽  
Control Group ◽  
Albino Rats ◽  
Standard Group ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Objective: The objective of this study was to evaluate the protective effect of Erdosteine on complete freund’s adjuvant (CFA) induced arthritic rats. Methods: Wistar Albino rats of 100–250 g were divided into five groups (n=6) and administered with 0.1 ml of CFA subcutaneously into the left hind paw except the negative control group. The standard group received methotrexate (MTX) 0.075 mg/kg body weight orally. Besides, the test groups received Erdosteine orally at a dose 10 mg/kg and 20 mg/kg bodyweight for 12 days. The changes in body weight, paw volume, hematological parameters, radiographical, and histological findings were the indicators to evaluate the efficacy of the test product. Discussion: Significant change in the body weight, paw volume, radiographical, hematological, and histological parameters were observed which supports the remarkable reduction of the arthritic development in the standard and test groups compared to the untreated group. However, the test group (Erdosteine) with the dose 20 mg/kg shows to be more potent than the test group (Erdosteine) with a dose 10 mg/kg and the standard group (MTX) to reduce the arthritic effect. Results: The test group with 20 mg/kg Erdosteine showed much better outcome than the standard group at significant (p<0.05). Therefore, Erdosteine acting as an anti-inflammatory and anti-oxidant is effective at a dose 20 mg/kg in treating the progression of rheumatoid arthritis in rats.

scholarly journals Evidence for anti-viral effects of complete Freund’s adjuvant in the mouse model of enterovirus infection

2020 ◽  
Author(s):  
Arunakumar Gangaplara ◽  
Chandirasegaran Massilamany ◽  
Ninaad Lasrado ◽  
David Steffen ◽  
Jay Reddy
Keyword(s):  
Viral Infections ◽  
Complete Freund’S Adjuvant ◽  
Control Group ◽  
Cell Responses ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Group B ◽  
Viral Protein 1 ◽  
Adjuvant Effects

AbstractGroup B Coxsackieviruses belonging to the genus, Enterovirus, contain six serotypes that induce various diseases, whose occurrence may involve the mediation of more than one serotype. We recently identified immunogenic epitopes within CVB3 viral protein 1 that induce anti-viral T cell responses in mouse models of CVB infections. In our investigations to determine the protective responses of the viral epitopes, we unexpectedly noted that animals immunized with complete Freund’s adjuvant (CFA) alone and later challenged with CVB3 were completely protected against myocarditis. Similarly, the pancreatitis-inducing ability of CVB3 was remarkably reduced to only 10% in the CFA group as opposed to 73.3% in the control group that received no CFA. Additionally, no mortalities were noted in the CFA group, whereas 40% of control animals died during the course of 21 days post-infection with CVB3. Taken together, our data suggest that the adjuvant effects of CFA may be sufficient for protection against CVB infections. These observations may provide new insights into our understanding of the occurrence of viral infections. One example is Coronavirus disease-19 (COVID-19) as individuals suffering from COVID-19 who have been vaccinated with Bacillus Calmette–Guérin appear to have fewer morbidities and mortalities than unvaccinated individuals.

scholarly journals Aktivitas Anti-inflamasi Ekstrak Etanol Daun Beluntas (Pluchea indica L.) pada Model Inflamasi Terinduksi CFA (Complete Freund's Adjuvant)

2017 ◽  
Vol 3 (2) ◽  
pp. 191-198
Author(s):  
Reza Setiawan Sudirman ◽  
Usmar Usmar ◽  
Abdul Rahim ◽  
Muh. Akbar Bahar
Keyword(s):  
Diclofenac Sodium ◽  
Negative Control ◽  
Complete Freund’S Adjuvant ◽  
Control Group ◽  
Edema Volume ◽  
Group I ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory

A research about anti-inflammatory effect of Beluntas leaves extract on CFA (Complete Freund’s Adjuvant) induced inflammatory model has been conducted. The objective of this research was to determine the effect of Beluntas leaves extract in alleviating CFA-induced paw edema in mice (Mus musculus). The number of mice used was 15 and was divided into 5 groups. Group I was treated with NaCMC. Group II, III, and IV were given suspension of Beluntas leaves extract 100 mg/Kg, 300 mg/Kg, and 500 mg/Kg BW, respectively. Group V was a positive control treated with suspension of diclofenac sodium 0.1 ml/10 g orally. The determination of anti-inflammatory potency was based on the average time needed to ameliorate the edema volume. The shortest  time period of edema reduction was produced by diclofenac sodium (within 9.33 days), then followed by Beluntas leaves extract with the concentration of 300 mg/Kg (within 12 days), 500 mg/Kg (within 14.33 days), and 100 mg/Kg (within 17.67 days), consecutively. These results are significantly different compared to negative control group which did not reduce the edema volume during 18 days of observation. In conclusion, ethanol extract of Beluntas leaves has an effective anti-inflamatory effect.

scholarly journals Evidence for Anti-Viral Effects of Complete Freund’s Adjuvant in the Mouse Model of Enterovirus Infection

Vaccines ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 364
Author(s):  
Arunakumar Gangaplara ◽  
Chandirasegaran Massilamany ◽  
Ninaad Lasrado ◽  
David Steffen ◽  
Jay Reddy
Keyword(s):  
Viral Infections ◽  
Complete Freund’S Adjuvant ◽  
Control Group ◽  
Cell Responses ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Group B ◽  
Viral Protein 1 ◽  
Adjuvant Effects

Group B coxsackieviruses (CVBs) belonging to the genus, Enterovirus and contain six serotypes that induce various diseases, whose occurrence may involve the mediation of more than one serotype. We recently identified immunogenic epitopes within coxsackieviruses B3 (CVB3) viral protein 1 that induce anti-viral T cell responses in mouse models of CVB infections. In our investigations to determine the protective responses of the viral epitopes, we unexpectedly noted that animals immunized with complete Freund’s adjuvant (CFA) alone and later challenged with CVB3 were completely protected against myocarditis. Similarly, the pancreatitis-inducing ability of CVB3 was remarkably reduced to only 10% in the CFA group as opposed to 73.3% in the control group that received no CFA. Additionally, no mortalities were noted in the CFA group, whereas 40% of control animals died during the course of 21 days post-infection with CVB3. Taken together, our data suggest that the adjuvant effects of CFA may be sufficient for protection against CVB infections. These observations may provide new insights into our understanding of the occurrence of viral infections.

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