scholarly journals Hesperidin and eugenol attenuate cadmium-induced nephrotoxicity via regulation of oxidative stress, Bax/Bcl2 and cleaved caspase 3 expression

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Rasha Hussein ◽  
Marwa Khalaf ◽  
Wafaa Mohamed
Keyword(s):  
Oxidative Stress ◽  
Caspase 3 ◽  
Kidney Tissue ◽  
Treated Group ◽  
Protective Effects ◽  
Tissue Integrity ◽  
Malondialdehyde Content ◽  
Male Wistar Rats ◽  
Cellular Antioxidants ◽  
Apoptotic Effects

AbstractObjectivesCadmium (Cd) is a heavy metal that induces nephrotoxicity through stimulating the oxidative stress in kidney cells. In this study, we investigated the protective effects of hesperidin and eugenol on Cd-induced nephrotoxicity and their impact on modulating oxidative stress, Bax/Bcl2 ratio and cleaved caspase 3 level.MethodsMale Wistar rats were divided into: Normal group; Cd-treated group; hesperidin & Cd-treated group and eugenol & Cd-treated group. Rats received CdCl2 (5 mg/kg body weight/daily) via oral gavage for four weeks to induce kidney toxicity.ResultsThe results showed that, both hesperidin & Cd and eugenol & Cd- treated groups exhibited lower serum creatinine and urea levels as well as better kidney tissue integrity compared to the Cd-treated group. Moreover, they could preserve the cellular antioxidants to normal levels as was seen in a significantly lower malondialdehyde content, but significantly higher catalase and superoxide dismutase activities compared to the Cd-treated group. Furthermore, both groups significantly decreased the Cd-induced elevation in Bax/Bcl2 ratio and cleaved caspase 3 level.ConclusionAdministration of hesperidin or eugenol effectively protected from the structural and functional kidney damage induced by Cd exposure via antioxidant and anti-apoptotic effects.

scholarly journals Protective Effects of Echium amoenum on Oxidative Stress and Gene Expression Induced by Permethrin in Wistar Rats

2020 ◽  
Vol 20 (6) ◽  
Author(s):  
Reza Abbasi Larki ◽  
Ehsan Zayerzadeh ◽  
Naser Harzandi ◽  
Ali Anissian
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Glutathione Peroxidase ◽  
Wistar Rats ◽  
Treated Group ◽  
Protective Effects ◽  
Cytoprotective Effect ◽  
High Concentration ◽  
Male Wistar Rats ◽  
Echium Amoenum

Background: Echium amoenum (E. amoenum), as one of the most popular plants in Iran, is traditionally used to treat different types of disorders. Objectives: This experimental study aimed to evaluate the modulatory effects of E. amoenum on permethrin (PMN)-induced oxidative stress in rats and to determine the cytoprotective effect of E. amoenum on PMN in SK-Hep-1 cells. Methods: Twenty-four male Wistar rats were randomly divided into four equal groups, including the control (normal saline), orally treated PMN (125 mg/kg of PMN), E. amoenum (100 mg/kg), and E. amoenum + PMN groups for 28 days. The levels of alanine aminotransferase (ALT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lipid peroxidation (LPO), catalase (CAT), and glutathione peroxidase (GPx), as well as the expression of catalase (CAT) and glutathione peroxidase (GPx), were measured in the liver of all rats. Also, the cytoprotective effect of E. amoenum against PMN was evaluated in the treated SK-Hep-1 cells. Results: The results indicated that LPO increased significantly in the PMN-treated group, as evidenced by the high concentration of malondialdehyde (MDA) in the liver. Alterations of the antioxidant system were also confirmed by the significant decline in CAT and GPx activities (2.9 ± 0.14 and 0.5 ± 0.03, respectively; P < 0.05) and the significant downregulation of CAT (0.4 ± 0.02 folds) and GPx (0.3 ± 0.01 folds) mRNA expression in the liver (P < 0.05). PMN also stimulated significant changes in hepatic biomarkers and induced pathological changes in the liver. On the other hand, administration of E. amoenum significantly reduced abnormalities in biochemical markers, LPO, antioxidant enzymes, gene expression, and pathological complications induced by PMN (P < 0.05). E. amoenum also exhibited cytoprotective effects against cytotoxicity induced by PMN in SK-Hep-1 cells. Conclusions: The present results demonstrated that E. amoenum has significant antioxidant, gene-regulating, and cytoprotective effects.

scholarly journals Aerobic exercise and octopamine protects against deep-frying oil-induced cardiomyocyte apoptosis through modulation of caspase and pro-caspase 3

2020 ◽  
Author(s):  
Pantea kianmehr ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Parvin Farzanegi
Keyword(s):  
Aerobic Exercise ◽  
Caspase 3 ◽  
Treated Group ◽  
Heart Tissue ◽  
Frying Oil ◽  
Deep Frying ◽  
Male Wistar Rats ◽  
Apoptotic Effects ◽  
Stain Analysis ◽  
Toxic Byproduct

Abstract Background : Deep-frying is a common cooking method in which cooking is accompanied by carcinogenic byproducts such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation, which has been implicated in pulmonary, cardiac, and neurodegenerative diseases. This study aimed to explore the effect of aerobic exercise and octopamine on caspase 3 and pro-caspase 3 expression levels in the heart tissue of rat exposed deep-frying oil. Methods : 30 male Wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) deep-frying oil (DFO), 3) deep-frying oil+exercise (DFO+EXE), 4) deep-frying oil+octopamine (DFO+OCT), and 5) deep-frying oil+exercise+octopamine (DFO+EXE+OCT). The apoptotic effects of DFO in heart tissue were examined by TUNEL assay. Masson's trichrome stain used to study cardiomyocytes’ fibers. Moreover, caspase 3 and pro-caspase 3 genes and proteins expression in all groups were evaluated using quantitative real-time PCR and western blotting method, respectively. Results : Data showed a significant increase in apoptotic cells in the DFO-treated group ( P <0.05). Masson's trichrome stain analysis demonstrated that the number of cardiomyocytes' fibers are decreased, and collagen deposition is increased in the DFO group. In comparison, the collagen percentage was significantly reduced in exercise, OCT, and exercise+OCT groups. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decreased in deep-frying oil+exercise+OCT group ( P <0.05). Conclusions : The results of our study show that DFO lead to programmed cell death via the activation of caspase in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improves heart tissue by significantly decrease the expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.

scholarly journals The effects of aerobic exercise training with octopamine supplementation on cardiomyocyte apoptosis induced by deep frying oil: the role of caspase and pro-Caspase 3

2020 ◽  
Author(s):  
Pantea kianmehr ◽  
Mohammad Ali Azarbayjani ◽  
Maghsoud Peeri ◽  
Parvin Farzanegi
Keyword(s):  
Aerobic Exercise ◽  
Caspase 3 ◽  
Treated Group ◽  
Heart Tissue ◽  
Frying Oil ◽  
Aerobic Exercise Training ◽  
Expression Level ◽  
Deep Frying ◽  
Male Wistar Rats ◽  
Apoptotic Effects

Abstract Background: Deep frying is a common cooking method, in which cooking is accompanied by carcinogenic by-products such as acrolein. Acrolein is a toxic byproduct of lipid peroxidation, which has been implicated in pulmonary, cardiac, and neurodegenerative diseases. The aim of this study was to explore the effect of aerobic exercise and octopamine on caspase 3 and pro-caspase 3 expression level in heart tissue of rat exposed deep frying oil.Methods: 30 male wistar rats were divided into 5 groups (n=6 in each) including 1) control (CO), 2) deep frying oil (DFO), 3) deep frying oil + exercise (DFO+EXE), 4) deep frying oil + octopamine (DFO+OCT), and 5) deep frying oil + exercise + octopamine (DFO+EXE+OCT). The apoptotic effects of DFO in heart tissue were examined by TUNEL assay. Masson's trichrome stain used to study cardiomyocytic fibers. Moreover, caspase 3 and pro-caspase 3 genes and proteins expression in all groups was evaluated using quantitative real-time PCR and western blotting method, respectively. Results: Data showed a significant increase in apoptotic cells in the DFO-treated group (P <0.05). In Masson's Trichrome stain analysis demonstrated more cardiomyocytic fibers degradation and aggregation lymphocytic cells in DFO that exercise and OCT signisicantly improve this degradation. Also, the expression level of caspase 3 and pro-caspase 3 was significantly decrease in deep frying oil + exercise + octopamine group (P <0.05). Conclusions: According to the result of the current study, it can be assumed that DFO can lead to programmed cell death via the activation of caspase in heart tissue. However, it seems that aerobic exercise with octopamine supplementation improve heart tissue by significantly decrease expression of caspase 3 and pro-caspase 3 that inhibit apoptosis.

Abstract 352: Real-time Intravital Optical Imaging Reflects the Dynamic Changes of Oxidative Stress Induced by Cigarette Smoking in Vasculatures

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Ji Bak Kim ◽  
Jiheun Ryu ◽  
Joon Woo Song ◽  
Dong Joo Oh ◽  
DaeGab Gweon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cigarette Smoking ◽  
Optical Imaging ◽  
Real Time ◽  
Treated Group ◽  
Fluorescence Signal ◽  
Ros Production ◽  
Protective Effects ◽  
Dynamic Changes

Background: Reactive oxygen species (ROS) play a central role in cigarette smoking-induced atherogenesis. The present study aims to assess the smoking-induced acute oxidative stress within vasculatures, and evaluates whether the resveratrol, a natural polyphenol antioxidant, can counteract this ROS production, using a customized, high resolution intravital optical imaging in real-time. Methods and Results: 20-week-old male C57BL/6 mice were divided into four groups according to the preceding administration of resveratrol (R) (25mg/kg via gavage, for 7 days) and exposure to cigarette smoke (CS). To in vivo assess acute oxidative stress in blood vessels, dihydroethidium, which forms a red fluorescence (ethidium, excitation/emission: 520nm/610nm) upon reaction with ROS, was injected intraperitoneally. During CS exposure, temporal changes of fluorescence signals from the mouse cremaster muscle including vasculatures were assessed by intravital optical imaging for 15 minutes. Fluorescence signals were much more pronounced in CS exposed mice than controls (p<0.001). Resveratrol p.o. significantly reduced the CS-induced ROS signals compared to the non-treated group (fluorescence signal to noise ratio, SNR, 2.51±0.09 vs. 12.52±2.116, p=0.0002) (Figure A). Without CS exposure, fluorescence signals in targeted vasculatures were very low showing no difference between groups (SNR, 1.65±0.19 vs. 1.53±0.07, p=0.80) (Figure A). Lipid peroxidation was increased in CS group and significantly attenuated in resveratrol-treated mice (Figure B). Fluorescence microscopy and immunostainings corroborated the in vivo findings. Conclusions: The intravital optical imaging was able to in vivo estimate the dynamic changes of ROS production by CS exposure. Our data demonstrated that even a brief exposure to CS increased oxidative stress in vasculatures promptly, and the resveratrol exerts protective effects against the CS-induced acute oxidative stress.

Supplementation of kaempferol to in vitro maturation medium regulates oxidative stress and enhances subsequent embryonic development in vitro

Zygote ◽  
2019 ◽  
Vol 28 (1) ◽  
pp. 59-64
Author(s):  
Yuhan Zhao ◽  
Yongnan Xu ◽  
Yinghua Li ◽  
Qingguo Jin ◽  
Jingyu Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Membrane Potential ◽  
Mitochondrial Membrane Potential ◽  
Mitochondrial Membrane ◽  
Caspase 3 ◽  
Treated Group ◽  
Control Group ◽  
Oxygen Species

SummaryKaempferol (KAE) is one of the most common dietary flavonols possessing biological activities such as anticancer, anti-inflammatory and antioxidant effects. Although previous studies have reported the biological activity of KAE on a variety of cells, it is not clear whether KAE plays a similar role in oocyte and embryo in vitro culture systems. This study investigated the effect of KAE addition to in vitro maturation on the antioxidant capacity of embryos in porcine oocytes after parthenogenetic activation. The effects of kaempferol on oocyte quality in porcine oocytes were studied based on the expression of related genes, reactive oxygen species, glutathione and mitochondrial membrane potential as criteria. The rate of blastocyst formation was significantly higher in oocytes treated with 0.1 µm KAE than in control oocytes. The mRNA level of the apoptosis-related gene Caspase-3 was significantly lower in the blastocysts derived from KAE-treated oocytes than in the control group and the mRNA expression of the embryo development-related genes COX2 and SOX2 was significantly increased in the KAE-treated group compared with that in the control group. Furthermore, the level of intracellular reactive oxygen species was significantly decreased and that of glutathione was significantly increased after KAE treatment. Mitochondrial membrane potential (ΔΨm) was increased and the activity of Caspase-3 was significantly decreased in the KAE-treated group compared with that in the control group. Taken together, these results suggested that KAE is beneficial for the improvement of embryo development by inhibiting oxidative stress in porcine oocytes.

Effect of alpha-ketoglutarate and N-acetyl cysteine on cyanide-induced oxidative stress mediated cell death in PC12 cells

2010 ◽  
Vol 26 (5) ◽  
pp. 297-308 ◽  
Author(s):  
RM Satpute ◽  
J. Hariharakrishnan ◽  
R. Bhattacharya
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Pc12 Cells ◽  
Caspase 3 ◽  
Critical Role ◽  
Total Antioxidant Status ◽  
Protective Effects ◽  
Simultaneous Treatment ◽  
Acetyl Cysteine

Cyanide is a mitochondrial poison, which is ubiquitously present in the environment. Cyanide-induced oxidative stress is known to play a key role in mediating the neurotoxicity and cell death in rat pheochromocytoma (PC12) cells. PC12 cells are widely used as a model for neurotoxicity assays in vitro. In the present study, we investigated the protective effects of alpha-ketoglutarate (A-KG), a potential cyanide antidote, and N-acetyl cysteine (NAC), an antioxidant against toxicity of cyanide in PC12 cells. Cells were treated with various concentrations (0.625—1.25 mM) of potassium cyanide (KCN) for 4 hours, in the presence or absence of simultaneous treatment of A-KG (0.5 mM) and NAC (0.25 mM). Cyanide caused marked decrease in the levels of cellular antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR). Lipid peroxidation indicated by elevated levels of malondialdehyde (MDA) was found to be accompanied by decreased levels of reduced glutathione (GSH) and total antioxidant status (TAS) of the cells. Cyanide-treated cells showed notable increase in caspase-3 activity and induction of apoptotic type of cell death after 24 hours. A-KG and NAC alone were very effective in restoring the levels of GSH and TAS, but together they significantly resolved the effects of cyanide on antioxidant enzymes, MDA levels, and caspase-3 activity. The present study reveals that combination of A-KG and NAC has critical role in abbrogating the oxidative stress-mediated toxicity of cyanide in PC12 cells. The results suggest potential role of A-KG and NAC in cyanide antagonism.

scholarly journals Sulodexide Protects Contrast-Induced Nephropathy in Sprague-Dawley Rats

2016 ◽  
Vol 40 (3-4) ◽  
pp. 621-632 ◽  
Author(s):  
Qing Zhao ◽  
Jianyong Yin ◽  
Zeyuan Lu ◽  
Yiwei Kong ◽  
Guangyuan Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caspase 3 ◽  
Vehicle Group ◽  
Control Group ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Sprague Dawley ◽  
Contrast Induced Nephropathy ◽  
Sprague Dawley Rats ◽  
Hk2 Cells

Background: Sulodexide is a powerful antithrombin agent with reno-protective property. However, whether it has beneficial effects on Contrast-Induced Nephropathy (CIN) remained elusive. In the current study, we evaluated the therapeutic effects of Sulodexide on CIN and investigated the potential mechanisms. Methods: CIN model was induced by intravenous injection of indomethacin, followed by Ioversol and L-NAME. Sprague-Dawley rats were divided into 4 groups: control group, CIN group, CIN+vehicle group (CIN rats pretreated with vehicle) and CIN+ Sulodexide (CIN rats pretreated with Sulodexide). Sulodexide or an equivalent volume of vehicle was intravenously delivered 30 min before the induction of CIN. All the animals were sacrificed at 24h after CIN and tissues were harvested to evaluate renal injury, kidney oxidative stress and apoptosis levels. Plasma antithrombin III (ATIII) activities were also measured. Results: Compared to the untreated CIN group, improved renal function, reduced tubular injury, decreased levels of oxidative stress and apoptosis were observed in CIN rats receiving Sulodexide injection. In addition, we also found that ATIII activity was significantly higher in Sulodexide-administered group than that in vehicle-injected CIN rats. For in vitro studies, HK2 cells were exposed to Ioversol and the cyto-protective effects of Sulodexide were also determined. Sulodexide pretreatment protected HK2 cells against the cytotoxicity of Ioversol via inhibiting caspase-3 activity. Preincubation with Sulodexide could also attenuate H2O2-induced increases in ROS, apoptosis and caspase-3 levels. Conclusions: Taken together, Sulodexide could protect against CIN through activating ATIII, and inhibiting oxidative stress, inflammation and apoptosis.

scholarly journals Comparison of Protective Effects of Melatonin and Amifostine on Radiation-Induced Renal Oxidative Stress in Rats

2020 ◽  
Vol 71 (1) ◽  
pp. 2005
Author(s):  
S. CAKINA ◽  
T. GULYASAR ◽  
A. ÖZEN ◽  
S. PARLAR ◽  
Z. CUKUR ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Kidney Tissue ◽  
Spectrophotometric Analysis ◽  
Stress Index ◽  
Protective Effects ◽  
Tissue Samples ◽  
Total Antioxidant ◽  
Female Wistar Rats ◽  
Radiation Induced ◽  
Radiotherapy Alone

In this study, we aimed to compare the protective effects of melatonin and amifostine on radiation-induced oxidative stress. Fifty female Wistar rats (3-4 months old, weighing 200±25 g) were divided into five groups (with ten rats each) and treated as follows: control (Cont), radiotherapy alone (RT), radiotherapy + amifostine (RT+AMI), radiotherapy + melatonin (RT+MEL), radiotherapy + amifostine + melatonin (RT+AMI+MEL). Rats were irradiated individually with a single dose of 8 Gy and amifostine (200 mg/kg) and melatonin (10 mg/kg) was administered to rats 30 minutes before irradiation. At the end of this follow-up period (72 hours) the rats were sacrificed. Spectrophotometric Analysis has been performed to kidney tissue samples. As a result of statistical comparison between groups after RT, total antioxidant capacity (TAC) decreased, total oxidant status (TOS) and oxidative stress index (OSI) increased, although the statistically significant change was only for OSI (p = 0.030). Addition of AMI or MEL to RT increased TAC and OSI significantly (p = 0.000), but there was no additive effect for TAC and OSI when both drugs were given together (p = 1.000, p = 0.172, respectively). In terms of TOS, statistically significant increasing was only for AMI (p = 0.000). There was protective effect when both drugs were given together against on Radiation-Induced Renal Oxidative Stress.

scholarly journals Effect of Nandrolone Treatment with And Without Resistance Training on Superoxide Dismutase Concentration and Pathology of Kidney Tissue in Rats

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Esfandiyar Heidari ◽  
Seyed Ali Hosseini ◽  
Mohammad Ali Azarbayjani
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Resistance Training ◽  
Anabolic Steroids ◽  
Kidney Tissue ◽  
Sod Activity ◽  
Significant Difference ◽  
Stress Levels ◽  
Sh Group ◽  
Male Wistar Rats

Background: The prevalence of anabolic steroids abuse in athletes and non-athletes is associated with the risk of injury to various organs, but there are limited studies of oxidative changes in kidney tissue following nandrolone (N) administration. Objectives: the aim of this study was to investigate the effect of N treatment with and without resistance training (RT) on superoxide dismutase (SOD) concentration and tissue pathology of kidney tissue in rats. Methods: In this experimental study, 20 male Wistar rats were randomly divided into four groups of five rats including 1) control (C), 2) sham (normal saline) (Sh), 3) N, and 4) N + RT. Groups 3 and 4 received 10 mg/kg N peritoneally, and the N + RT group performed 1 m ladder climbing for eight weeks and three sessions per week. SOD levels of kidney tissue were measured by ELISA and radioimmunoassay. Hematoxylin-eosin (H&E) staining was used to evaluate oxidative stress levels in kidney tissue. One-way ANOVA with Bonferroni’s post- hoc tests were used for analysis of research findings in SPSS version 22 (P ≤ 0.05). Results: SOD levels in the C group were higher than the Sh (P = 0.001), N (P = 0.001), and N + RT (P = 0.001) groups. SOD levels were lower in the Sh group than in the N (P = 0.049) and N + RT (P = 0.001) groups. However, there was no significant difference in SOD levels in the N + RT group and N group (P = 0.28). Also, oxidative stress levels were normal in tissue studies in all groups. Conclusions: It seems that Ntreatment with and without RT reduces SOD activity in kidney tissue, but more studies are needed in this regard given the normality of tissue oxidative stress results.

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