Trehalose Protects Yeast Pyrophosphatase against Structural and Functional Damage Induced by Guanidinium Chloride

1996 ◽  
Vol 51 (3-4) ◽  
pp. 160-164 ◽  
Author(s):  
Mauro Sola-Penna ◽  
José Roberto Meyer-Fernandes
Keyword(s):  
Protective Effect ◽  
Degradation Product ◽  
Stress Condition ◽  
Stress Conditions ◽  
The Other ◽  
Guanidinium Chloride ◽  
Nium Chloride ◽  
Pyrophosphatase Activity ◽  
High Concentrations ◽  
Very High

Abstract Trehalose is accumulated at very high concentrations in yeasts when this organism is sub­ mitted to a stress condition. This report approaches the question on the protective effect of trehalose and its degradation product, glucose, against structural and functional damage promoted by guanidinium on yeast cytosolic pyrophosphatase. Here it is shown that both, 1 ᴍ trehalose or 2 ᴍ glucose, are able to attenuate at almost the same extent the conforma­ tional changes promoted by guanidinium chloride on the pyrophosphatase structure. On the other hand, while 1 m trehalose increases 3.8 times the Ki (from 0.15 to 0.57 ᴍ) for guanidi­ nium chloride inhibition of pyrophosphatase activity, 2 m glucose did not even duplicate this parameter (from 0.15 to 0.25 ᴍ). These data support evidences for a functional reason for the accumulation by yeasts of trehalose, and not other compound, during stress conditions.

Contrast Medium Induced Changes in Granulocyte Adherence in Vitro and during Angiography

1988 ◽  
Vol 29 (5) ◽  
pp. 585-588 ◽  
Author(s):  
E. V. Lang ◽  
E. C. Lasser
Keyword(s):  
Contrast Medium ◽  
Human Body ◽  
Indirect Effect ◽  
Femoral Vein ◽  
The Other ◽  
High Concentrations ◽  
Induced Changes ◽  
Very High

The effect of ioxaglate and diatrizoate on per cent granulocyte adherence to nylon fibers was investigated in blood to which contrast medium was added in vitro and in blood from patients undergoing angiography. Very high concentrations of contrast medium, added to blood in vitro, directly abolished granulocyte adherence to nylon fibers. Intraaortic bolus injections of ioxaglate, but not of saline, transiently increased granulocyte concentrations in the femoral vein. Fractional granulocyte adherence to nylon fibers increased significantly above the baseline when angiographic dosages of contrast medium were diluted by circulation within the human body. On the other hand, dilute concentrations of contrast medium had no effect on per cent granulocyte adherence when added to whole blood in vitro. This indicates that the increased adherence produced in vivo is an indirect effect, which, usually, cannot be simulated in vitro.

scholarly journals NEW MUTATIONAL VARIANTS OF NEUROSPORA NADP-SPECIFIC GLUTAMATE DEHYDROGENASE

Genetics ◽  
1980 ◽  
Vol 95 (2) ◽  
pp. 305-316
Author(s):  
John A Kinsey ◽  
J R S Fincham ◽  
M A M Siddig ◽  
Margaret Keighren
Keyword(s):  
Amino Acid ◽  
Glutamate Dehydrogenase ◽  
The Other ◽  
Tryptophan Residue ◽  
High Concentrations ◽  
Very High

ABSTRACT The am locus of Neurospora codes for NADP-dependent glutamate dehydrogenase (GDH). Four new am mutants that produced mutationally altered GDH have been characterized. Mutant am  119 is a CRM-negative, complementing mutant that maps between am  2 and am  1. The other three mutants are CRM formers that produce varieties of GDH that can be activated by glutamate or succinate. The GDH of am  130 and am  l31 is similar in terms of activation properties to that of am  3. The GDH of am  l22, requires very high concentrations of dicarboxylate for activity. The mutation in am  l30 maps between am  l4 and am  2 and resulted in a replacement at residue 75 of the GDH (pro→ser). The mutation in am  l22 maps near am  ll and apparently resulted in the replacement of the tryptophan residue at position 389 with an unknown amino acid. The mutation in am  l31 maps between am  2 and am  1.

The Kinetics and Mechanism of a Reaction in Which Ligand Substitution is Accompanied by a Spin-State Change: Observation of the Elusive Monodithiocarbamatonickel(II) Complex in Dimethyl Sulfoxide

1979 ◽  
Vol 32 (8) ◽  
pp. 1679 ◽  
Author(s):  
PJ Nichols ◽  
MW Grant
Keyword(s):  
Dimethyl Sulfoxide ◽  
Electronic Spectrum ◽  
Ligand Substitution ◽  
The Other ◽  
Direct Reaction ◽  
State Change ◽  
Low Concentrations ◽  
High Concentrations ◽  
Dimerization Reaction ◽  
Very High

In solutions in Me2SO containing low concentrations of diethyldithiocarbamate ion (dtc-) and very high concentrations of Ni(Me2SO)62+ ion, the hitherto undetected Ni(dtc)+ complex is kinetically stabilized and its electronic spectrum can be recorded. The mono complex is thermodynamically unstable and is converted into Ni(dtc)2 by two independent pathways, one involving direct reaction between Ni(dtc)+ and dtc-, the other being a dimerization reaction, 2Ni(dtc)+ → Ni2+ + Ni(dtc)2. Analysis of the mechanistic scheme Ni2+ + dtc ↔ Ni(dtc)2 Ni(dtc)+ + dtc- → Ni(dtc)2 2Ni(dtc)+ → Ni2+ + Ni(dtc)2 gives k1 = 3 × 103 l, mol-1 s-1, k-1 ≈ 0.01 s-1, k2 ≈ 4 × 105 l, mol-1 s-1 and k3 ≈ 1 × 103 l. mol-1 s-1. Coordinated dtc- exerts a significant labilizing effect on coordinated Me2SO molecules in Ni(dtc)+.

Thrombotic Thrombocytopenic Purpura: Mechanism for Effectiveness of Plasmapheresis

1979 ◽  
Author(s):  
J. G. Kelton ◽  
P. B. Neame ◽  
I. Walker ◽  
A. G. Turpie ◽  
J. McBride ◽  
...  
Keyword(s):  
Platelet Count ◽  
Thrombotic Thrombocytopenic Purpura ◽  
The Other ◽  
Unknown Etiology ◽  
Normal Range ◽  
Antiplatelet Antibody ◽  
Thrombocytopenic Purpura ◽  
Serious Illness ◽  
Normal Platelet ◽  
Very High

Thrombotic thrombocytopenic purpura (TTP) is a rare but serious illness of unknown etiology. Treatment by plasmapheresis has been reported to be effective but the mechanism for benefit is unknown. We have investigated the effect of plasmapheresis in 2 patients with TTP by quantitating platelet associated IgG (PAIgG) levels prior to and following plasmapheresis. Both patients had very high levels of PAIgG at presentation (90 and A8 fg IgG/platelet respectively, normal 0-5). in both, the PAIgG levels progressively fell to within the normal range and the platelet count rose following plasmapheresis. One patient remained in remission with normal platelet counts and PAIgG levels. The other relapsed after plasmapheresis and the PAIgG level rose prior to the fall in platelet count. Plasmapheresis was repeated and resulted in normalization of both the platelet count and PAIgG level. It is suggested that plasmapheresis removes antiplatelet antibody or immune complexes which may be of etiological importance in this illness.

Growth Characteristics of Activated Sludges Acclimated to para-Nitrophenol in Batch and Continuous Modes

1994 ◽  
Vol 29 (7) ◽  
pp. 327-333
Author(s):  
Y. Matsui ◽  
F. Yamaguchi ◽  
Y. Suwa ◽  
Y. Urushigawa
Keyword(s):  
Growth Characteristics ◽  
The Other ◽  
Operational Mode ◽  
Continuous Mode ◽  
Relative Resistance ◽  
Batch Mode ◽  
The Given ◽  
Operational Modes ◽  
Very High ◽  
Sensitive Group

Activated sludges were acclimated to p-nitrophenol (PNP) in two operational modes, a batch and a continuous. The operational mode of the PNP acclimation of activated sludges strongly affected the physiological characteristics of predominant microorganisms responsible for PNP degradation. Predominant PNP degraders in the sludge in batch mode (Sludge B) had lower PNP affinity and were relatively insensitive to PNP concentration. Those of the sludge in continuous mode (Sludge C), on the other hand, had very high PNP affinity and were sensitive to PNP. MPN enumeration of PNP degraders in sludge B and C using media with different PNP concentrations (0.05, 0.2,0.5 and 2.0 mM) supported the above results. Medium with 0.2 mM of PNP did not recover PNP degraders in sludge C well, while it recovered PNP degraders in sludge B as well as the medium with 0.05 mM did. When switching from one operational mode to the other, the predominant population in sludge B shifted to the sensitive group, but that of sludge C did not shift at the given loading of PNP, showing relative resistance to inhibitive concentration.

The metachor as a characteristic of the association of electrolytes in aqueous solutions

1984 ◽  
Vol 49 (5) ◽  
pp. 1061-1078 ◽  
Author(s):  
Jiří Čeleda ◽  
Stanislav Škramovský
Keyword(s):  
Aqueous Solutions ◽  
Apparent Volume ◽  
Solutions Of Electrolytes ◽  
Molal Volumes ◽  
The Difference ◽  
High Concentrations ◽  
Experimental Values ◽  
Suitable Characteristic ◽  
Association Of Ions ◽  
Very High

Based on the earlier paper introducing a concept of the apparent parachor of a solute in the solution, we have eliminated in the present work algebraically the effect which is introduced into this quantity by the additivity of the apparent molal volumes. The difference remaining from the apparent parachor after substracting the contribution corresponding to the apparent volume ( for which the present authors suggest the name metachor) was evaluated from the experimental values of the surface tension of aqueous solutions for a set of 1,1-, 1,2- and 2,1-valent electrolytes. This difference showed to be independent of concentration up to the very high values of the order of units mol dm-3 but it was directly proportional to the number of the free charges (with a proportionality factor 5 ± 1 cm3 mol-1 identical for all studied electrolytes). The metachor can be, for this reason, a suitable characteristic for detection of the association of ions and formation of complexes in the solutions of electrolytes, up to high concentrations where other methods are failing.

Islamic hedging for pilgrimage funds: case of Indonesia

2019 ◽  
Vol 11 (3) ◽  
pp. 328-341
Author(s):  
Rifki Ismal ◽  
Nurul Izzati Septiana
Keyword(s):  
Exchange Rate ◽  
The Other ◽  
Exchange Rate Risk ◽  
Content Type ◽  
Other Hand ◽  
Rate Risk ◽  
Risk Identify ◽  
Hajj Season ◽  
The Right ◽  
Very High

Purpose The demand for Saudi Arabian real (SAR) is very high in the pilgrimage (hajj) season while the authority, unfortunately, does not hedge the hajj funds. As such, the hajj funds are potentially exposed to exchange rate risk, which can impact the value of hajj funds and generate extra cost to the pilgrims. The purpose of this paper is to conduct simulations of Islamic hedging for pilgrimage funds to: mitigate and minimize exchange rate risk, identify and recommend the ideal time, amount and tenors of Islamic hedging for hajj funds, estimate cost saving by pursuing Islamic hedging and propose technical and general recommendations for the authority. Design/methodology/approach Forward transaction mechanism is adopted to compute Islamic forward between SAR and Rupiah (Indonesian currency) or IDR. Findings – based on simulations, the paper finds that: the longer the Islamic hedging tenors, the better is the result of Islamic hedging, the decreasing of IDR/USD is the right time to hedge the hajj funds and, on the other hand, the IDR/SAR appreciation is not the right time to hedge the hajj funds. Findings Based on simulations, the paper finds that: the longer the Islamic hedging tenors, the better is the result of Islamic hedging, the decreasing of IDR/USD is the right time to hedge the hajj funds and, on the other hand, the IDR/SAR appreciation is not the right time to hedge the hajj funds. Research limitations/implications The research suggests the authority to (and not to) hedge the hajj fund, depending on economic conditions and market indicators. Even though the assessment is for the Indonesian case, other countries maintaining hajj funds might also learn from this paper. Originality/value To the best of author’s knowledge, this is the first paper in Indonesia that attempts to simulate the optimal hedging of hajj funds.

scholarly journals Nitrification Process in a Nuclear Wastewater with High Load of Nitrogen, Uranium and Organic Matter under ORP Controlled

Water ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1607
Author(s):  
Mariano Venturini ◽  
Ariana Rossen ◽  
Patricia Silva Paulo
Keyword(s):  
Ammonia Oxidation ◽  
High Load ◽  
Nuclear Fuels ◽  
Monod Model ◽  
Nernst Equation ◽  
Nitrification Process ◽  
Real Wastewater ◽  
High Concentrations ◽  
Wastewater Samples ◽  
Very High

To produce nuclear fuels, it is necessary to convert uranium′s ore into UO2-ceramic grade, using several quantities of kerosene, methanol, nitric acid, ammonia, and, in low level, tributyl phosphate (TBP). Thus, the effluent generated by nuclear industries is one of the most toxic since it contains high concentrations of dangerous compounds. This paper explores biological parameters on real nuclear wastewater by the Monod model in an ORP controlled predicting the specific ammonia oxidation. Thermodynamic parameters were established using the Nernst equation to monitor Oxiders/Reductors relationship to obtain a correlation of these parameters to controlling and monitoring; that would allow technical operators to have better control of the nitrification process. The real nuclear effluent is formed by a mixture of two different lines of discharges, one composed of a high load of nitrogen, around 11,000 mg/L (N-NH4+-N-NO3−) and 600 mg/L Uranium, a second one, proceeds from uranium purification, containing TBP and COD that have to be removed. Bioprocesses were operated on real wastewater samples over 120 days under controlled ORP, as described by Nernst equations, which proved to be a robust tool to operate nitrification for larger periods with a very high load of nitrogen, uranium, and COD.

scholarly journals Metals in Calluna vulgaris, Empetrum nigrum, Festuca vivipara and Thymus praecox ssp. arcticus in the geothermal areas of Iceland

2021 ◽  
Author(s):  
Adam Rajsz ◽  
Bronisław Wojtuń ◽  
Aleksandra Samecka-Cymerman ◽  
Paweł Wąsowicz ◽  
Lucyna Mróz ◽  
...  
Keyword(s):  
Active Sites ◽  
Calluna Vulgaris ◽  
Bioaccumulation Factor ◽  
The Other ◽  
Extreme Situation ◽  
Empetrum Nigrum ◽  
Geothermal Activity ◽  
High Concentrations ◽  
And Control ◽  
Festuca Vivipara

AbstractThis investigation was conducted to identify the content of metals in Calluna vulgaris (family Ericaceae), Empetrum nigrum (family Ericaceae), Festuca vivipara (family Poaceae) and Thymus praecox subsp. arcticus (family Lamiaceae), as well as in the soils where they were growing in eight geothermal heathlands in Iceland. Investigation into the vegetation of geothermal areas is crucial and may contribute to their proper protection in the future and bring more understanding under what conditions the plants respond to an ecologically more extreme situation. Plants from geothermally active sites were enriched with metals as compared to the same species from non-geothermal control sites (at an average from about 150 m from geothermal activity). The enriched metals consisted of Cd, Co, Cu, Fe and Ni in C. vulgaris; Cd, Mn and Ti in E. nigrum; Hg and Pb in F. vivipara; and Cd, Fe and Hg in T. praecox. Notably, C. vulgaris, E. nigrum, F. vivipara and T. praecox had remarkably high concentrations of Ti at levels typical of toxicity thresholds. Cd and Pb (except for C. vulgaris and F. vivipara) were not accumulated in the shoots of geothermal plants. C. vulgaris from geothermal and control sites was characterised by the highest bioaccumulation factor (BF) of Ti and Mn; E. nigrum and F. vivipara by the highest BF of Ti and Cr; and T. praecox by the highest BF of Ti and Zn compared to the other elements. In comparison with the other examined species, F. vivipara from geothermal sites had the highest concentration of Ti in above-ground parts at any concentration of plant-available Ti in soil.

scholarly journals Halogenated Volatile Organic Compounds in Water Samples and Inorganic Elements Levels in Ores for Characterizing a High Anthropogenic Polluted Area in the Northern Latium Region (Italy)

2021 ◽  
Vol 18 (4) ◽  
pp. 1628
Author(s):  
Mario Vincenzo Russo ◽  
Ivan Notardonato ◽  
Alberto Rosada ◽  
Giuseppe Ianiri ◽  
Pasquale Avino
Keyword(s):  
Organic Compounds ◽  
Mean Value ◽  
Greenhouse Crops ◽  
River Waters ◽  
Liquid Liquid Extraction ◽  
Volatile Organic ◽  
High Concentrations ◽  
Very High ◽  
Inorganic Fraction

This paper shows a characterization of the organic and inorganic fraction of river waters (Tiber and Marta) and ores/soil samples collected in the Northern Latium region of Italy for evaluating the anthropogenic/natural source contribution to the environmental pollution of this area. For organic compounds, organochloride volatile compounds in Tiber and Marta rivers were analyzed by two different clean-up methods (i.e., liquid–liquid extraction and static headspace) followed by gas chromatography–electron capture detector (GC-ECD) analysis. The results show very high concentrations of bromoform (up to 1.82 and 3.2 µg L−1 in Tiber and Marta rivers, respectively), due to the presence of greenhouse crops, and of chloroform and tetrachloroethene, due to the presence of handicrafts installations. For the qualitative and quantitative assessment of the inorganic fraction, it is highlighted the use of a nuclear analytical method, instrumental neutron activation analysis, which allows having more information as possible from the sample without performing any chemical-physical pretreatment. The results have evidenced high levels of mercury (mean value 88.6 µg g−1), antimony (77.7 µg g−1), strontium (12,039 µg g−1) and zinc (103 µg g−1), whereas rare earth elements show levels similar to the literature data. Particular consideration is drawn for arsenic (414 µg g−1): the levels found in this paper (ranging between 1 and 5100 µg g−1) explain the high content of such element (as arsenates) in the aquifer, a big issue in this area.

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