Induction of Phenolic Compounds in Wheat (Triticum aestivum L.) Tissue Cultures by Streptomycin

2007 ◽  
Vol 62 (1-2) ◽  
pp. 50-54 ◽  
Author(s):  
Atiat M. A. Hassan
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Experimental Period ◽  
Triticum Aestivum L ◽  
Stationary Growth Phase ◽  
Total Phenolic ◽  
Ms Medium ◽  
Tissue Cultures ◽  
Mature Embryos ◽  
Pal Activity

The tissue cultures of wheat (Triticum aestivum L.) were induced from the mature embryos (explants) of the dry grains and grown on MS medium containing kinetin (0.1 mg/l) and 2,4 D (1.0 mg/l). The cultures were incubated for two weeks at (25 ± 2) °C under a light/dark regime (16 h light daily). The formed calli were subcultured at the beginning of the stationary growth phase (15 days) with fresh MS medium containing 0, 5, 10, 25, 50, 100, 150 mg/l streptomycin elicitor and maintained for two weeks for three subcultures. A significant increase in phenylalanine ammonia lyase (PAL) activity coincided with the increase of the total phenolic compounds after elicitation with streptomycin. Maximum induction was recorded during the first two weeks, then gradually declined during the rest of the experimental period, but the values attained were still markedly higher than that of the control. The endogenous cinnamic acid content was also increased significantly with the increase in PAL activity making about 2-18% of the total phenolic acids. The growth and accumulation of phenolic compounds were inversely related. However, accumulation of phenolic compounds became limited for growth of wheat tissue culture especially during the long term cultivation.

Effect of the time and temperature of germination on the phenolic compounds of Triticum aestivum, L. and Panicum miliaceum, L.

LWT ◽  
2020 ◽  
Vol 127 ◽  
pp. 109396 ◽  
Author(s):  
Dayana Ceccaroni ◽  
Vincenzo Alfeo ◽  
Elisabetta Bravi ◽  
Valeria Sileoni ◽  
Giuseppe Perretti ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Triticum Aestivum L ◽  
Panicum Miliaceum

Effect of arachidonic and jasmonic acid elicitation on the content of phenolic compounds and antioxidant and anti-inflammatory properties of wheatgrass (Triticum aestivum L.)

2019 ◽  
Vol 288 ◽  
pp. 256-261 ◽  
Author(s):  
Urszula Złotek ◽  
Urszula Szymanowska ◽  
Anna Jakubczyk ◽  
Małgorzata Sikora ◽  
Michał Świeca
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Jasmonic Acid ◽  
Triticum Aestivum L ◽  
Anti Inflammatory

Silicon carbide fiber-mediated DNA delivery into cells of wheat ( Triticum aestivum L.) mature embryos

1996 ◽  
Vol 16 (3-4) ◽  
pp. 133-136 ◽  
Author(s):  
Serik Omirulleh *, † , Ainur Ismagul ◽  
Tetsuo Meshi ◽  
Masaki Iwabuchi
Keyword(s):  
Silicon Carbide ◽  
Triticum Aestivum ◽  
Dna Delivery ◽  
Triticum Aestivum L ◽  
Silicon Carbide Fiber ◽  
Mature Embryos

scholarly journals Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)

2021 ◽  
Vol 50 (4) ◽  
pp. 889-896
Author(s):  
Samih M. Tamimi ◽  
Halima Othman
Keyword(s):  
Triticum Aestivum ◽  
Callus Induction ◽  
High Performance ◽  
Triticum Aestivum L ◽  
Mature Embryo ◽  
Callus Cultures ◽  
Differentiation Potential ◽  
Mature Embryos ◽  
The Mean ◽  
Wheat Callus

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating embryos compared to their soaked counterpart (24 to 28%), suggesting a better differentiation potential of callus cultures derived from germinating embryo. These findings indicate that germinating mature embryo is more suitable explant for wheat callus induction and regeneration than the soaked mature embryo commonly employed for wheat callus culture.

Formation of phenolic compounds in various cultivars of wheat (Triticum aestivum L.)

2005 ◽  
Vol 41 (1) ◽  
pp. 99-102 ◽  
Author(s):  
N. V. Zagoskina ◽  
N. A. Olenichenko ◽  
Chzhou Yun?vei ◽  
E. A. Zhivukhina
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Triticum Aestivum L

scholarly journals Elicitation-Based Method for Increasing the Production of Antioxidant and Bactericidal Phenolic Compounds in Dionaea muscipula J. Ellis Tissue

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1794 ◽  
Author(s):  
Wojciech Makowski ◽  
Krzysztof Michał Tokarz ◽  
Barbara Tokarz ◽  
Rafał Banasiuk ◽  
Karolina Witek ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bactericidal Activity ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Total Phenolic ◽  
Tissue Cultures ◽  
Venus Flytrap ◽  
Dionaea Muscipula ◽  
Plant Culture

The carnivorous plant Dionaea muscipula J. Ellis (Venus flytrap) is a widely known medical herb, capable of producing various phenolic compounds known for their strong antioxidant and antibacterial properties. In the pharmaceutical industry, Venus flytrap is grown in tissue cultures, as the natural population of D. muscipula is very limited. Here, we describe an improved method to increase the quantity and quality of phenolic compounds produced in D. muscipula. This is achieved by combining biotic elicitation (using Cronobacter sakazakii bacteria lysate) of D. muscipula cultured with rotary shaking (hydromechanical stress), which we describe here for the first time. The antibacterial activity and the antioxidant properties of the obtained compounds were studied on two antibiotic-resistant human pathogenic bacteria. The proposed plant culture conditions resulted in an increase in fresh weight, as well as a higher total phenolic content, in comparison to traditional tissue cultures on agar-solidified medium. With the use of high-performance liquid chromatography, we demonstrated that the described elicitation strategy leads to an increased synthesis of myricetin, caffeic acid, ellagic acid and plumbagin in D. muscipula tissue. We also found that a higher level of antioxidant activity, exhibited by the plant extract, corresponded with its higher phenylpropanoid content. The bactericidal activity of the extract against Staphylococcus aureus was dependent on the duration of plant culture under described elicitation conditions, whereas neither elicitation condition (duration or elicitor concentration) seemed relevant for the bactericidal activity of the extract towards Escherichia coli. This suggest that Gram-negative bacteria are less sensitive to compounds derived from Venus flytrap tissue.

scholarly journals Modulation of Physiological Stress Response of Triticum aestivum L. to Glyphosate by Brassinosteroid Application

Life ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1156
Author(s):  
Elena Shopova ◽  
Zornitsa Katerova ◽  
Liliana Brankova ◽  
Ljudmila Dimitrova ◽  
Iskren Sergiev ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Phenolic Compounds ◽  
Winter Wheat ◽  
Antioxidant Defense ◽  
Physiological Stress ◽  
Physiological Responses ◽  
Triticum Aestivum L ◽  
Guaiacol Peroxidase ◽  
Herbicide Application ◽  
Wheat Seedlings

The potential of brassinosteroids to modulate the physiological responses of winter wheat (Triticum aestivum L.) to herbicide stress was evaluated. Young winter wheat seedlings were treated with 24-epibrassinolide (EBL) and 24 h later were sprayed with glyphosate. The physiological responses of treated plants were assessed 14 days after herbicide application. Wheat growth was noticeably inhibited by glyphosate. The herbicide application significantly increased the content of the stress markers proline and malondialdehyde (MDA) evidencing oxidative damage. The content of phenolic compounds was decreased in the herbicide-treated plants. Slight activation of superoxide dismutase (SOD) and catalase (CAT) and considerable increase of glutathione reductase (GR) and guaiacol peroxidase (POX) activities were found. Increased POX and glutathione S-transferase (GST) activities were anticipated to be involved in herbicide detoxification. Conjugation with glutathione in herbicide-treated plants could explain the reduction of thiols suggesting unbalanced redox state. EBL application did not alter the plant growth but a moderate activation of antioxidant defense (POX, GR, and CAT activities and phenolic levels) and detoxifying enzyme GST was observed. The hormonal priming provoked a slight decrease in MDA and proline levels. The results demonstrate that EBL-pretreatment partly restored shoot growth and has a potential to mitigate the oxidative damages in glyphosate-treated plants through activation of the enzymatic antioxidant defense and increase of the phenolic compounds.

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