THE EFFECT OF CORTISONE AND ANABOLIC STEROIDS ON THE RETENTION OF RADIOACTIVE CALCIUM AND STRONTIUM IN RATS

ABSTRACT Experiments with adult rats treated with dexamethasone showed a decreased retention of radioactive calcium and strontium as compared with the control animals. In addition a marked reduction in weight was observed probably due to dehydration. In animals which had additional injections of nortestosterone compounds, i. e. Durabolin® and Anadur® respectively, these effects of cortisone were not counteracted although animals treated with nortestosterone compounds alone showed an increased retention of radioactive calcium as compared with the control animals.

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Anabolic steroids in part reverse glucocorticoid-induced alterations in rat diaphragm

Journal of Applied Physiology ◽

10.1152/jappl.1998.84.5.1492 ◽

1998 ◽

Vol 84 (5) ◽

pp. 1492-1499 ◽

Cited By ~ 19

Author(s):

Roland H. H. Van Balkom ◽

P. N. Richard Dekhuijzen ◽

Hans T. M. Folgering ◽

Jacques H. Veerkamp ◽

Herman T. Van Moerkerk ◽

...

Keyword(s):

Low Dose ◽

Anabolic Steroids ◽

Acid Oxidation ◽

Type I ◽

Adult Rats ◽

Cross Sectional ◽

Male Adult ◽

Reduction In Force ◽

Beneficial Effects

Animal and clinical studies have shown respiratory muscle dysfunction caused by treatment with glucocorticoids. The present study was designed to investigate whether anabolic steroids are able to antagonize the loss of diaphragm force induced by long-term low-dose methylprednisolone (MP) administration. Male adult rats were randomized to receive saline or MP (0.2 mg ⋅ kg−1 ⋅ day−1sc) during 9 mo, with or without nandrolone decanoate (ND; 1 mg ⋅ kg−1 ⋅ wk−1im) during the last 3 mo. The ∼10% reduction in force generation of isolated diaphragm bundles induced by MP was completely abolished by addition of ND. The MP-induced decrease in number of fibers expressing type IIb myosin heavy chains was not reversed by ND. MP slightly reduced type I, IIa, and IIx fiber cross-sectional areas (CSA), but not type IIb fiber CSA. Addition of ND abolished the reduction in IIa and IIx fiber CSA. The MP-induced alterations in glycogenolytic activity and fatty acid oxidation capacity were not reversed by ND. In conclusion, the marked reduction in diaphragm force caused by long-term low-dose MP was completely abolished by addition of ND. ND in part also antagonized the effects of MP on diaphragm morphology but showed no beneficial effects on biochemical changes.

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Further Studies on the Ultrastructure of Harderian Gland in Adult Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100051451 ◽

1974 ◽

Vol 32 ◽

pp. 288-289

Author(s):

Alfredo Feria-Velasco ◽

Guadalupe Tapia-Arizmendi

Keyword(s):

Fine Structure ◽

Domestic Fowl ◽

Animal Species ◽

Acinar Cells ◽

Harderian Gland ◽

Myoepithelial Cells ◽

The Other ◽

Albino Rats ◽

Adult Rats ◽

Cell Components

The fine structure of the Harderian gland has been described in some animal species (hamster, rabbit, mouse, domestic fowl and albino rats). There are only two reports in the literature dealing on the ultrastructure of rat Harderian gland in adult animals. In one of them the author describes the myoepithelial cells in methacrylate-embbeded tissue, and the other deals with the maturation of the acinar cells and the formation of the secretory droplets. The aim of the present work is to analize the relationships among the acinar cell components and to describe the two types of cells located at the perifery of the acini.

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Biodegradable polyester neuroprostheses promote the reconnection of separated peripheral nerve stubs

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130109 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1094-1095

Author(s):

Beverly L. Giammara ◽

Jennifer S. Stevenson ◽

Peggy E. Yates ◽

Robert H. Gunderson ◽

Jacob S. Hanker

Keyword(s):

Image Analysis ◽

Basement Membrane ◽

Periodic Acid ◽

Light And Electron Microscopy ◽

Computer Assisted ◽

Type Iii Collagen ◽

Image Analysis System ◽

Biodegradable Polyester ◽

Adult Rats ◽

Type Iv

An 11mm length of sciatic nerve was removed from 10 anesthetized adult rats and replaced by a biodegradable polyester Vicryl™ mesh sleeve which was then injected with the basement membrane gel, Matrigel™. It was noted that leg sensation and movement were much improved after 30 to 45 days and upon sacrifice nerve reconnection was noted in all animals. Epoxy sections of the repaired nerves were compared with those of the excised segments by the use of a variation of the PAS reaction, the PATS reaction, developed in our laboratories for light and electron microscopy. This microwave-accelerated technique employs periodic acid, thiocarbohydrazide and silver methenamine. It stains basement membrane or Type IV collagen brown and type III collagen (reticulin), axons, Schwann cells, endoneurium and perineurium black. Epoxy sections of repaired and excised nerves were also compared by toluidine blue (tb) staining. Comparison of the sections of control and repaired nerves was done by computer-assisted microscopic image analysis using an Olympus CUE-2 Image Analysis System.

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Surface Ultrastructure of Human Ectocervix in Certain Pathological Conditions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119636 ◽

1985 ◽

Vol 43 ◽

pp. 570-571

Author(s):

S. Mukherjee ◽

T. Guha ◽

B. Chakrabarti ◽

P. Chakrabarti

Keyword(s):

Epithelial Cells ◽

Marked Reduction ◽

Squamous Epithelium ◽

Malignant Tumour ◽

Surface Ultrastructure ◽

Normal Tissues ◽

Pathological Conditions ◽

Hexagonal Shape ◽

Columnar Cells ◽

Scanning Electron

The cervix is an important organ in reproduction. Its malfunction is frequently a factor for infertility. Ectocervix region does not appear to have received much attention although many studies have been reported on the endocervix. We report here our SEM observations on ectocervix in certain pathological conditions compared to normal ectocervix.Ectocervix specimens from human females with specific pathological disorders were processed for Scanning Electron Microscopy by conventional method and they were examined in a Philips SEM.The normal ectocervix is lined by flat layer of squamous epithelial cells with microridges (Fig. 1). These cells are known to be formed from columnar cells through metaplastic transformation. The cells of carcinoma-bearing ectocervix show a disorganised appearance (Fig. 2). In non-malignant tumour surface some cuboidal and few columnar cells were seen (Fig. 3). A cyst appears like an overgrowth on the surface of the squamous epithelium (Fig. 4). In ulcerated ectocervix a marked reduction of epithelial cells are observed (Fig. 5); the cells are devoid of microridges and, the large polygonal cells, as observed in normal tissues, have somehow acquired comparatively small hexagonal shape

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High-voltage EM description of the types and distribution of sensory endings in the inner conical body of the rat vibrissal follicle-sinus complex

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015959x ◽

1990 ◽

Vol 48 (3) ◽

pp. 410-411

Author(s):

Tony M. Mosconi ◽

Min J. Song ◽

Frank L. Rice

Keyword(s):

Mammalian Species ◽

Dorsal Side ◽

Hair Shaft ◽

Sensory Innervation ◽

Conical Body ◽

Adult Rats ◽

Perpendicular Plane ◽

Ultrastructural Studies ◽

Unmyelinated Axons ◽

Sensory Endings

Whiskers or vibrissal follicle-sinus complexes (F-SCs) on the snouts of many mammalian species are structures that have complex, dense sensory innervation. The innervation of F-SCs is remarkably similar in all species with the exception of one site - the inner conical body (ICB). The ICB is an elongated cylindrical structure that encircles the hair shaft near the neck of the follicle. This site has received only cursory attention in ultrastructural studies of the F-SCAdult rats were perfused after the method of Renehan and Munger2. F-SCs were quartered longitudinally and embedded separately in Epon-Araldite. Serial 0.25 μm sections were cut in either the longitudinal or perpendicular plane through the ICB and examined with an AEI EM7 1.2 MV HVEM (Albany, NY) at 1000 KV. Sensory endings were reconstructed from serial micrographs through at least 20 μm in the longitudinal plane and through 10 μm in the perpendicular plane.From two to six small superficial vibrissal nerves converge upon the neck of the F-SC and descend into the ICB. The nerves branch into smaller bundles of myelinated and unmyelinated axons along the dorsal side of the hair shaft.

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