Effects of luteinizing hormone-releasing hormone (LRH) upon bioactive and immunoreactive serum LH in patients with Turner's syndrome before and after oestrogen treatment

1985 ◽  
Vol 109 (3) ◽  
pp. 304-308 ◽  
Author(s):  
Marco Francesco Celani ◽  
Vanna Montanini ◽  
Paolo Marrama
Keyword(s):  
Biologically Active ◽  
Delayed Response ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Oestrogen Treatment ◽  
Serum Lh ◽  
Before And After ◽  
The Mean ◽  
Ethinyl Oestradiol

Abstract. One daily dose of 0.05 mg ethinyl oestradiol was administered to 5 patients with Turner's syndrome (mean age ± sem = 16.4 ± 0.7 years) for 10 days. The effects of acute stimulation with luteinizing hormonereleasing hormone (LRH) (0.1 mg iv) on biologically active and immunoreactive LH were analysed before therapy and at the end of oestrogen treatment. Bioactive LH (BIO-LH) was measured by a sensitive and specific in vitro bioassay based upon testosterone production by mechanically dispersed mouse Leydig cell preparations. Immunoreactive LH (RIA-LH) was evaluated by a double antibody RIA method. Prior to oestrogen treatment, LRH induced a prompt rise in BIO-LH and RIA-LH levels, which reached peak values at 30 and 45 min, respectively. After oestrogen treatment, a delayed response (with peak values at 120 min) was observed for both BIO-LH and RIA-LH. Before oestrogen treatment, the mean bioactivity to immunoreactivity (B/I) ratio of LRH-stimulated LH showed a significant decrease from basal values (P < 0.05). In contrast, after ethinyl oestradiol administration the mean LH B/I ratio increased significantly from basal values in response to LRH (P < 0.05). The mean relative maximum response (Δ%) for BIO-LH was significantly higher (P < 0.05) in oestrogen-treated than in untreated patients, whereas the mean BIO-LH Δ area was significantly lower in the former group (P < 0.01). Similarly, oestrogens decreased significantly the mean RIA-LH Δ area (P < 0.05), whereas they did not affect significantly the mean RIA-LH Δ%. The results further emphasize that oestrogens may change the quality of circulating LH.

OESTROGEN TREATMENT AND SUBSEQUENT PREGNANCY IN TWO PATIENTS WITH SEVERE HYPERGONADOTROPHIC OVARIAN FAILURE

1978 ◽  
Vol 89 (1) ◽  
pp. 149-157 ◽  
Author(s):  
J. Starup ◽  
J. Philip ◽  
V. Sele
Keyword(s):  
Ovarian Failure ◽  
Subsequent Pregnancy ◽  
Premature Menopause ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Substitution Therapy ◽  
Oestrogen Treatment

ABSTRACT This report describes in detail the histological and hormonal findings in a patient with Turner's syndrome (45,XO) and a patient with premature menopause (46,XX), who both conceived after withdrawal or reduction of substitution therapy with oestrogens. The aetiology of severe hypergonadotrophic ovarian failure is discussed, and theories regarding a possible relationship between the oestrogen treatment and subsequent pregnancy are hypothesized.

Oestrogen treatment of adolescent tall girls; short term side effects

1986 ◽  
Vol 113 (4_Suppl) ◽  
pp. S170-S173 ◽  
Author(s):  
OLAV TRYGSTAD
Keyword(s):  
Side Effects ◽  
Idiopathic Scoliosis ◽  
Final Height ◽  
Psychosocial Problems ◽  
Short Term ◽  
Oestrogen Treatment ◽  
Duration Of Therapy ◽  
Tall Girls ◽  
The Mean ◽  
Ethinyl Oestradiol

Abstract In 1980-1985 680 preadolescent tall girls were treated with pharmacological doses of oestrogen to reduce final height. Indications for the therapy were predicted final height >+2.5 SD (180.75 cm), idiopathic scoliosis, and psychosocial problems. Until 1976 141 girls were given diethyl stilboestrol 5 mg daily. By advice of Prader this was then replaced by ethinyl oestradiol and a progestin was given on days 5-10 each month. The mean duration of therapy was close to 2 years. The observed short-term unwanted effects were due to the pharmacological actions of the drugs, (11 girls had galactorrhoea at the end of therapy; no pituitary prolactionoma was observed) or events happening by chance.

Serum LH and FSH Responses to Synthetic LH-RH in Normal Infants, Children and Patients With Turner's Syndrome

PEDIATRICS ◽  
1974 ◽  
Vol 54 (4) ◽  
pp. 470-475
Author(s):  
Selzo Suwa ◽  
Hatae Maesaka ◽  
Ichiro Matsui
Keyword(s):  
Normal Subjects ◽  
Serum Levels ◽  
Young Female ◽  
Normal Female ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Normal Children ◽  
Serum Lh ◽  
Female Children ◽  
Lh Rh

Effects of LH-RH on LH and FSH release were studied in 26 normal children and six patients with Turner's syndrome (two of them showed 45 x karyotype and the others were mosaics). Synthetic LH-RH (2µg/kg of body weight) was given intramuscularly after an overnight fast. The increase of serum FSH level was significantly greater in normal female infants than male infants. A similar tendency was observed in normal female children aged 2 to 9 years. No sex difference was observed in the LH response to LH-RH in all the normal subjects Studied. The response of FSH release to LH-RH was significantly greater than that of LH in female infants and young female children. The responsiveness of LH to LH-RH gradually increased with advancing age. Basal serum levels of FSH rather than LH were high in the patients with Turner's syndrome. Again a much greater increase of serum FSH than of LH was noted after the administration of LH-RH to patients with Turner's syndrome.

scholarly journals Serum Levels of 20-Kilodalton Human Growth Hormone (GH) Are Parallel Those of 22-Kilodalton Human GH in Normal and Short Children

1999 ◽  
Vol 84 (1) ◽  
pp. 98-104 ◽  
Author(s):  
Mayumi Ishikawa ◽  
Susumu Yokoya ◽  
Katsuhiko Tachibana ◽  
Yukihiro Hasegawa ◽  
Toshiaki Yasuda ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Short Stature ◽  
Physiological Role ◽  
Growth Disorders ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Nocturnal Sleep ◽  
Normal Children ◽  
Significant Difference ◽  
The Mean

Twenty-kilodalton human GH (20K), which is one of the human GH (hGH) variants, is thought to be produced by alternative premessenger ribonucleic acid splicing. However, its physiological role is still unclear due to the lack of a specific assay. We have measured serum 20K and 22-kDa hGH (22K) by specific ELISAs to investigate the physiological role of 20K in children. The subjects were 162 normal children, aged 1 month to 20 yr; 12 patients with GH deficiency (GHD), aged 11 months to 13 yr; 57 children with non-GHD short stature, aged 2–17 yr; and 13 girls with Turner’s syndrome, aged 5 months to 15 yr. Samples were collected at random from normal children and were collected after hGH provocative tests and 3-h nocturnal sleep from GHD, non-GHD short stature, and Turner’s syndrome children. The mean basal serum concentrations of 22K and 20K were 2.4 ± 2.8 ng/mL and 152.3 ± 184.0 pg/mL in normal boys and 2.5 ± 3.1 ng/mL and 130.6 ± 171.5 pg/mL in normal girls, respectively. The percentages of 20K (%20K) were 5.8 ± 2.1% and 6.0 ± 3.2% in 83 normal boys and 79 normal girls, respectively. There was no significant difference in %20K either among ages or between the prepubertal stage and the pubertal stage in normal boys and girls. The mean %20K values in basal samples of provocative tests in 12 patients with GHD, non-GHD short stature, and Turner’s syndrome were 6.5 ± 2.4%, 6.5 ± 3.8%, and 5.9 ± 3.2%, respectively. There was no significant difference in %20K among normal children and these growth disorders, and there was no significant difference in %20K throughout the hGH provocative tests and 3-h nocturnal sleep in these growth disorders. There was also no significant correlation between the percentage of 20K and the height sd score or body mass index in either normal children or subjects with these growth disorders. In conclusion, the %20K is constant, regardless of age, sex, puberty, height sd score, body mass index, and GH secretion status. The regulation of serum 20K levels remains to be established.

Evaluation of chromatin integrity of motile bovine spermatozoa capacitated in vitro

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Z. Reckova ◽  
M. Machatkova ◽  
R. Rybar ◽  
J. Horakova ◽  
P. Hulinska ◽  
...  
Keyword(s):  
Chromatin Structure ◽  
Production Efficiency ◽  
Sperm Chromatin ◽  
Percoll Gradient ◽  
Embryo Production ◽  
Before And After ◽  
Bovine Spermatozoa ◽  
Chromatin Integrity ◽  
The Mean

SummaryThe efficiency of in vitro embryo production is highly variable amongst individual sires in cattle. To eliminate that this variability is not caused by sperm chromatin damage caused by separation or capacitacion, chromatin integrity was evaluated. Seventeen of AI bulls with good NRRs but variable embryo production efficiency were used. For each bull, motile spermatozoa were separated on a Percoll gradient, resuspended in IVF–TALP medium and capacitated with or incubated without heparin for 6 h. Samples before and after separation and after 3-h and 6-h capacitacion or incubation were evaluated by the Sperm Chromatin Structure Assay (SCSA) and the proportion of sperm with intact chromatin structure was calculated. Based on changes in the non-DFI-sperm proportion, the sires were categorized as DNA-unstable (DNA-us), DNA-stable (DNA-s) and DNA-most stable (DNA-ms) bulls (n = 3, n = 5 and n = 9, respectively). In DNA-us bulls, separation produced a significant increase of the mean non-DFI-sperm proportion (p ≤ 0.01), as compared with the value before separation. Capacitacion produced a significant decrease in the mean non-DFI-sperm proportion in H+ sperm (p ≤ 0.01). In DNA-s bulls, separation significantly increased the mean non-DFI-sperm proportion (p ≤ 0.01) but during capacitacion, the mean non-DFI-sperm proportion remained almost unchanged. In DNA-ms bulls, neither separation nor capacitacion had any effect on the mean non-DFI-sperm proportion. It can be concluded that, although separation and capacitacion may produce some changes in sperm chromatin integrity, these are not associated with different in vitro fertility of the bulls involved.

scholarly journals Growth hormone secretion in Turner's syndrome and influence of oxandrolone and ethinyl oestradiol.

1989 ◽  
Vol 64 (4) ◽  
pp. 587-592 ◽  
Author(s):  
A A Massarano ◽  
C G Brook ◽  
P C Hindmarsh ◽  
P J Pringle ◽  
J D Teale ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Turner's Syndrome ◽  
Turner’S Syndrome ◽  
Ethinyl Oestradiol

Effects of acute stimulation with luteinizing hormone-releasing hormone (LRH) on biologically active and immunoreactive serum luteinizing hormone (LH) in pubertal boys

1984 ◽  
Vol 107 (3) ◽  
pp. 289-294 ◽  
Author(s):  
Vanna Montanini ◽  
Marco Francesco Celani ◽  
Gian Franco Baraghini ◽  
Cesare Carani ◽  
Paolo Marrama
Keyword(s):  
Luteinizing Hormone ◽  
Serum Levels ◽  
Biologically Active ◽  
Acute Administration ◽  
Adult Men ◽  
Serum Luteinizing Hormone ◽  
Significant Difference ◽  
The Mean ◽  
Pubertal Boys

Abstract. The responses of biologically active LH (BIO-LH) and immunoreactive LH (RIA-LH) to acute stimulation with LRH (0.1 mg iv) were studied in 8 pubertal boys (9–15 years, 2nd–4th Tanner's stage), and in 10 healthy adult men (20–46 years). Serum levels of BIO-LH were assessed by an in vitro bioassay method based upon testosterone production by mechanically dispersed mouse Leydig cell preparations. In pubertal boys the mean BIO-LH/RIA-LH (B/I) ratio of basally secreted LH was significantly lower than in adult men (1.2 ± 0.2 (sem) and 2.2 ± 0.2 respectively, P < 0.01). After acute administration of LRH the mean B/I ratio of circulating LH showed a significant increase from the basal value in pubertal boys (2.6 ± 0.2, P < 0.01 vs basal values), whereas no significant difference in LH B/I ratios were demonstrated throughout the study period in adult men (2.1 ± 0.1, P = NS vs basal values). In agreement with this finding, the mean relative maximum response for BIO-LH (BIO-LH Δ%) was higher in pubertal boys than in adult men (1702.7 ± 500.3 and 499.6 ± 65.4% respectively, P < 0.05), whereas the mean RIA-LH Δ% was similar in both groups (609.1 ± 85.1 and 534.1 ± 75.5% respectively, P = NS). No significant differences were shown in the BIO-LH Δ area between pubertal boys (4.9 ± 0.9 area units × 103) and adult men (6.7 ± 1.2 area units × 103, P = NS), whereas the mean RIA-LH Δ area was significantly lower in the former group (1.9 ± 0.4 area units × 103 vs 3.2 ± 0.5 area units × 103, P < 0.05). Our study emphasizes that the pubertal pituitary possesses a greater responsiveness for BIO-LH than the adult pituitary, and that in pubertal boys acute stimulation with LRH evokes the release of a more bioactive form of LH.

scholarly journals Comparative in vitro study of the cleaning efficacy of AirFloss ultra and I-Prox Sulcus brushes in an orthodontic phantom model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hanna Boes ◽  
Sören Brüstle ◽  
Gholamreza Danesh ◽  
Stefan Zimmer ◽  
Mozhgan Bizhang
Keyword(s):  
In Vitro Study ◽  
White Spot Lesions ◽  
Mean Values ◽  
Phantom Model ◽  
Before And After ◽  
Tooth Surfaces ◽  
The Mean ◽  
Post Hoc ◽  
Cleaning Efficacy

AbstractPreventing biofilm-related risks such as gingivitis and white spot lesions during orthodontic treatments is very challenging. The cleaning efficiencies of AirFloss Ultra and I-Prox P sulcus brushes were evaluated using an orthodontic phantom model. After attaching brackets onto black-coated maxillary KaVo teeth, a plaque substitute was applied. The evaluated tooth surfaces were divided into two areas. Cleaning was performed with an AirFloss Ultra with two (A-2) or four (A-4) sprays or an I-Prox P for two (I-2) or four (I-4) seconds. Images before and after cleaning were digitally subtracted, and the percentage of fully cleaned surfaces was determined (Adobe Photoshop CS5, ImageJ). Statistical analysis was performed by ANOVA and post hoc tests with Bonferroni correction (SPSS 25, p < 0.05). The mean values of total cleaning efficacy were 26.87% for I-2, 43.73% for I-4, 34.93%, for A-2 and 56.78% for A-4. The efficacy was significantly higher for A-4 than for A-2, I-4, and I-2. There were significant differences between the four groups. Repeated cleaning led to an improved result. Within the study limitations, the AirFloss Ultra with four sprays proved to be more efficient than the sulcus brush I-Prox P for cleaning.

Oestrogenic regulation of testicular androgen production during development in the rat

1985 ◽  
Vol 105 (2) ◽  
pp. 211-218 ◽  
Author(s):  
B. A. Keel ◽  
T. O. Abney
Keyword(s):  
Serum Testosterone ◽  
Male Rats ◽  
Serum Prolactin ◽  
Testicular Function ◽  
Intact Male ◽  
Oestrogen Treatment ◽  
Testosterone Production ◽  
Age Related ◽  
Serum Lh

ABSTRACT The influence of age on the sensitivity of the testis to oestrogens was investigated. Intact male rats at 10, 25, 40 and 53 days of age were injected s.c. with vehicle, 5 or 50 μg oestradiol or diethylstilboestrol (DES)/100 g body wt twice daily for 2 days; the animals were killed 12 h after the last injection. Subsequently, the concentrations of testicular androgens and serum LH, prolactin, testosterone and androstanediol (5α-androstane-3α, 17β-diol) were measured. Testicular androgen production was determined in vitro in the presence or absence of human chorionic gonadotrophin (hCG) or dibutyryl cyclic AMP (dbcAMP). Androgens in the serum and testes displayed an age-related alternating pattern with androstanediol being the major androgen produced at 27 days of age. As a result of oestrogen treatment, serum LH concentrations were decreased while serum prolactin was increased. Serum testosterone was decreased by 36–55% in the 12-day-old group and further reduced by 95% of control values by day 55; serum androstanediol was less sensitive to oestrogen suppression. Testicular concentrations of both testosterone and androstanediol exhibited a marked reduction in 12-day-old animals as a result of oestrogen administration. These values were reduced by 85–95% at day 27 and remained suppressed even at 55 days. Basal production of testosterone was unaffected by oestrogen treatment in 12- and 27-day-old animals but was markedly decreased by day 42. Significant suppression of basal production of androstanediol was observed as early as day 12. Oestradiol treatment caused a significant reduction in hCG responsiveness of both androgens at days 12, 42 and 55. Oestrogen administration resulted in a significant (32–59%) decline in dbcAMP-responsive testosterone production in the 42-day group and a further suppression in the 55-day group. A marked inhibition of dbcAMP-stimulated androstanediol production was also observed in the 42- and 55-day groups. Testosterone production in response to dbcAMP was not significantly altered in the 12- and 27-day groups. With few exceptions the effects of oestradiol and DES on testicular function were similar. The data presented here suggest that the inhibitory effects of oestrogens become more pronounced as the animal approaches adulthood, that oestradiol and DES are similarly effective in regulating testicular function at all ages studied and that the production of both testosterone and androstanediol are suppressed by oestrogen administration. J. Endocr. (1985) 105, 211–218

scholarly journals Prospective Pilot Evaluation of the Efficacy and Safety of Topical Ingenol Mebutate Gel for Localized Patch/Plaque Stage Mycosis Fungoides

2017 ◽  
Vol 11 (1) ◽  
pp. 98-107 ◽  
Author(s):  
Eve Lebas ◽  
Charlotte Castronovo ◽  
Jorge E. Arrese ◽  
Florence Libon ◽  
Nazli Tassoudji ◽  
...  
Keyword(s):  
Mycosis Fungoides ◽  
Topical Treatment ◽  
Lesion Size ◽  
Efficacy And Safety ◽  
Ingenol Mebutate ◽  
T Cell Lymphomas ◽  
Before And After ◽  
The Mean ◽  
Neoplastic Lymphocytes

Background: Mycosis Fungoides (MF) is the most frequent type of the primary cutaneous NK/T-cell lymphomas. Ingenol mebutate (IM) displays in vitro pro-apoptotic properties on neoplastic lymphocytes. Objectives: To evaluate the efficacy and safety of IM gel as topical treatment for MF. Materials and Methods: Ten male patients with longstanding classic type MF (n=9) and follicular MF (FMF; n=1), T2bN0M0B0, stage Ib, resistant to systemic methotrexate or acitretin therapies for at least 3 months, were included in this pilot study. In these patients, 11 target patch/plaque stage lesions with an area ≤ 25 cm2 were selected for IM therapy (0,05%, 2 weekly applications). The primary endpoint was the improvement of the CAILS scores. Biopsies were performed before and after treatment from 10 target lesions. Relapse rates were evaluated at 6 months. Results: The mean CAILS score of treated target lesions was reduced by 58.2%. The mean erythema, scaling and plaque elevation scores were improved by 73.6%, 93.9% and 97.9% (p<0.0001), respectively, while the lesion size remained unchanged (p=0.34). A complete or partial clearance of histological and immunohistochemical features was observed in 6/10 (60%) and 4/10 (40%) of the MF or FMF target lesions, respectively. Monoclonal TCR rearrangement was evidenced in 100% (7/7) of the patients and in 3/7 (43%) after treatment. The relapse rate at 6 months was 18%. All the patients experienced burning sensations, oozing and crusting. Conclusion: IM gel warrants further investigation and development as a potential topical treatment for localized patch/plaque stage MF and FMF.

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