Vitamin D and its metabolites in human milk. Changes of concentration with duration of lactation

1986 ◽  
Vol 113 (1_Suppl) ◽  
pp. S196
Author(s):  
D. STALLMANN ◽  
S. ISSA ◽  
C. KUNZ ◽  
W. BURMEISTER
Keyword(s):  
Vitamin D ◽  
Human Milk

Water-Soluble Vitamin D in Human Milk: A Myth

PEDIATRICS ◽  
1982 ◽  
Vol 70 (3) ◽  
pp. 499-499
Author(s):  
Joseph A. Little
Keyword(s):  
Vitamin D ◽  
Human Milk ◽  
Clinical Observation ◽  
Water Soluble ◽  
University Of Wisconsin ◽  
Water Soluble Vitamin ◽  
The University ◽  
Breast Fed

I was happy to read the report from Greer et al.1 This biochemically confirms the clinical observation that the majority of children who develop clinical rickets are breast-fed. During the ten years, 1970-1979, I have seen six cases of clinical rickets. All of these infants were breast-fed.2 The only exception has been the rickets of prematurity. I should like to commend, again, the report from the Department of Pediatrics and Biochemistry of the University of Wisconsin.

scholarly journals Quantitative analysis of vitamin D and its main metabolites in human milk by supercritical fluid chromatography coupled to tandem mass spectrometry

2019 ◽  
Vol 412 (2) ◽  
pp. 365-375 ◽  
Author(s):  
J. M. Oberson ◽  
S. Bénet ◽  
K. Redeuil ◽  
E. Campos-Giménez
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Tandem Mass Spectrometry ◽  
Human Milk ◽  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Multiple Reaction Monitoring ◽  
Ammonium Formate ◽  
Tandem Mass ◽  
Relative Standard

AbstractA novel method to quantitate vitamin D and its main metabolites (vitamin D3, vitamin D2, and their 25-hydroxy metabolites) in breast milk by supercritical fluid chromatography has been developed and fully validated. A small volume of sample (1 mL) is subjected to ethanolic protein precipitation and liquid-liquid extraction. Final extracts are derivatized with 4-phenyl-1,2,4-triazoline-3,5-dione and vitamin D derivatives analyzed by supercritical fluid chromatography hyphenated to tandem mass spectrometry with atmospheric pressure chemical ionization. Multiple reaction monitoring is used for quantitation. Separation conditions were optimized using a gradient of methanol-water-ammonium formate into carbon dioxide. Make-up solvent was methanol containing ammonium formate. The quantitation limit reached levels as low as 50 pmol/L, with intra- and inter-day relative standard deviations lower than 15% and 20% for all analytes. Accuracy was evaluated by spiking experiments and was well within acceptability ratios (± 15%). The method was then applied to a subset of commercially available human milk samples. The newly developed method provides opportunities to determine the nutritional status of mother-infant dyads from a non-invasive measure, or for interventional or observational studies building knowledge on the composition of human milk.

Vitamin D metabolites in human milk

1982 ◽  
Vol 100 (5) ◽  
pp. 745-748 ◽  
Author(s):  
Yosef Weisman ◽  
Joseph C. Bawnik ◽  
Zipora Eisenberg ◽  
Zvi Spirer
Keyword(s):  
Vitamin D ◽  
Human Milk ◽  
Vitamin D Metabolites

PREMATURITY AND RICKETS

PEDIATRICS ◽  
1957 ◽  
Vol 20 (1) ◽  
pp. 63-77
Author(s):  
S. Eek ◽  
L. H. Gabrielsen ◽  
S. Halvorsen
Keyword(s):  
Vitamin D ◽  
Human Milk ◽  
Premature Infants ◽  
Premature Birth ◽  
Clinical Signs ◽  
Clinical Findings ◽  
Cow’S Milk ◽  
Cow's Milk ◽  
Adequate Supply ◽  
Calcium Phosphorus

Sixty-nine premature infants receiving different dietaries and with birth weights below 2,000 gm were studied with chemical, clinical and radiologic methods. Vitamin D was given to all infants from the age of 4 days. The concentrations of calcium, phosphorus and phosphatase in the serum showed variations toward those seen in active rickets. These changes were somewhat more marked in infants fed human milk than cow's milk. Supplementation of human milk with dried skimmed cow's milk did not significantly alter these changes. Craniotabes was more frequently seen in premature infants fed human milk than cow's milk. Otherwise, no clinical signs of rickets were found. All the premature infants were born with a metaphyseal zone of rarefaction. These zones are believed to be the result of a disturbance in ossification which occured during the last weeks of pregnancy, perhaps caused by a factor which was also responsible for the premature birth. They gradually merge with the general osteoporosis developing 10 to 13 weeks after birth. Later, periosteal double contours are visible. These double contours are the first signs of an improved mineralization. There was no correlation between abnormal chemical findings and the most conspicuous radiologic or clinical findings. No case of recognizable rickets was observed among the infants studied. This risk of rickets, despite an adequate supply of vitamin D, is probably not as great as stated by previous investigators, and is probably the same whether the infants are fed human milk or cow's milk.

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