scholarly journals 17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules

2011 ◽  
Vol 48 (2) ◽  
pp. 89-97 ◽  
Author(s):  
Camille Bois ◽  
Christelle Delalande ◽  
Hélène Bouraïma-Lelong ◽  
Philippe Durand ◽  
Serge Carreau
Keyword(s):  
Gene Expression ◽  
Male Gamete ◽  
Cyclin B1 ◽  
Seminiferous Tubules ◽  
Cyclin A1 ◽  
Adult Rat ◽  
Germ Cell Differentiation ◽  
Estrogen Synthesis ◽  
B1 Gene ◽  
17Β Estradiol

Spermatogenesis, which is the fundamental mechanism allowing male gamete production, is controlled by several factors, and among them, estrogens are likely concerned. In order to enlighten the potential role of estrogen in rat spermatogenesis, seminiferous tubules (ST) from two groups of seminiferous epithelium stages (II–VIII and IX–I) were treated with either 17β-estradiol (E2) agonists or antagonists for estrogen receptors (ESRs). In this study, we show that cyclin A1 and cyclin B1 gene expression is controlled by E2at a concentration of 10−9 M only in stages IX–I. This effect is mimicked by a treatment with the G-protein coupled estrogen receptor (GPER) agonist G1 and is abolished by treatment with the ESR antagonist ICI 182 780. Moreover, using letrozole, a drug that blocks estrogen synthesis, we demonstrate that these genes are under the control of E2within rat ST. Thus, germ cell differentiation may be regulated by E2which acts through ESRs and GPER, expressed in adult rat ST.

scholarly journals Network Analyses Predict Small RNAs That Might Modulate Gene Expression in the Testis and Epididymis of Bos indicus Bulls

2021 ◽  
Vol 12 ◽  
Author(s):  
Andressa O. de Lima ◽  
Juliana Afonso ◽  
Janette Edson ◽  
Esteban Marcellin ◽  
Robin Palfreyman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Rnas ◽  
Molecular Mechanisms ◽  
Bos Indicus ◽  
Male Gamete ◽  
Functional Enrichment ◽  
Pairwise Comparisons ◽  
Network Analyses ◽  
Germ Cell Differentiation ◽  
Network Cluster

Spermatogenesis relies on complex molecular mechanisms, essential for the genesis and differentiation of the male gamete. Germ cell differentiation starts at the testicular parenchyma and finishes in the epididymis, which has three main regions: head, body, and tail. RNA-sequencing data of the testicular parenchyma (TP), head epididymis (HE), and tail epididymis (TE) from four bulls (three biopsies per bull: 12 samples) were subjected to differential expression analyses, functional enrichment analyses, and co-expression analyses. The aim was to investigate the co-expression and infer possible regulatory roles for transcripts involved in the spermatogenesis of Bos indicus bulls. Across the three pairwise comparisons, 3,826 differentially expressed (DE) transcripts were identified, of which 384 are small RNAs. Functional enrichment analysis pointed to gene ontology (GO) terms related to ion channel activity, detoxification of copper, neuroactive receptors, and spermatogenesis. Using the regulatory impact factor (RIF) algorithm, we detected 70 DE small RNAs likely to regulate the DE transcripts considering all pairwise comparisons among tissues. The pattern of small RNA co-expression suggested that these elements are involved in spermatogenesis regulation. The 3,826 DE transcripts (mRNAs and small RNAs) were further subjected to co-expression analyses using the partial correlation and information theory (PCIT) algorithm for network prediction. Significant correlations underpinned the co-expression network, which had 2,216 transcripts connected by 158,807 predicted interactions. The larger network cluster was enriched for male gamete generation and had 15 miRNAs with significant RIF. The miRNA bta-mir-2886 showed the highest number of connections (601) and was predicted to down-regulate ELOVL3, FEZF2, and HOXA13 (negative co-expression correlations and confirmed with TargetScan). In short, we suggest that bta-mir-2886 and other small RNAs might modulate gene expression in the testis and epididymis, in Bos indicus cattle.

scholarly journals YB-1 as a Cell Cycle-regulated Transcription Factor Facilitating Cyclin A and Cyclin B1 Gene Expression

2003 ◽  
Vol 278 (30) ◽  
pp. 27988-27996 ◽  
Author(s):  
Karsten Jürchott ◽  
Stephan Bergmann ◽  
Ulrike Stein ◽  
Wolfgang Walther ◽  
Martin Janz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Cycle ◽  
Transcription Factor ◽  
Cyclin A ◽  
Cyclin B1 ◽  
B1 Gene ◽  
A Cell

scholarly journals Mxi1 inhibits the proliferation of U87 glioma cells through down-regulation of cyclin B1 gene expression

2002 ◽  
Vol 86 (3) ◽  
pp. 477-484 ◽  
Author(s):  
I Manni ◽  
P Tunici ◽  
N Cirenei ◽  
R Albarosa ◽  
B M Colombo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Glioma Cells ◽  
Cyclin B1 ◽  
Down Regulation ◽  
B1 Gene

scholarly journals Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 80
Author(s):  
Xin Li ◽  
Chenying Duan ◽  
Ruyi Li ◽  
Dong Wang
Keyword(s):  
Gene Expression ◽  
Semen Quality ◽  
Regulation Of Gene Expression ◽  
Physiological Mechanism ◽  
Interaction Network ◽  
Seminiferous Tubules ◽  
Differential Analysis ◽  
Epididymal Sperm ◽  
Bioinformatics Analyses ◽  
Acrosome Formation

To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional ART3 gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression.

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