The (pro)renin receptor and soluble (pro)renin receptor in choriocarcinoma

Reproduction ◽  
2021 ◽  
Author(s):  
Saije K Morosin ◽  
Sarah Jane Delforce ◽  
Richard G. S. Kahl ◽  
Celine Corbisier de Meaultsart ◽  
Eugenie R Lumbers ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Invasion ◽  
Cell Analysis ◽  
Invasive Potential ◽  
Cellular Invasion ◽  
Protein Levels ◽  
Hcg Secretion ◽  
Cytotrophoblast Cells ◽  
Choriocarcinoma Cells ◽  
Analysis System

This study aimed to determine if the (pro)renin receptor (ATP6AP2) changes the cellular profile of choriocarcinomas from cytotrophoblast cells to terminally syncytialised cells and ascertain whether this impacts on the invasive potential of choriocarcinoma cells. Additionally, we aimed to confirm that FURIN and/or site 1 protease (MBTPS1) cleave soluble ATP6AP2 (sATP6AP2) in BeWo choriocarcinoma cells and determine whether sATP6AP2 levels reflect the cellular profile of choriocarcinomas. BeWo choriocarcinoma cells were treated with ATP6AP2 siRNA, FURIN siRNA, DEC-RVKR-CMK (to inhibit FURIN activity) or PF 429242 (to inhibit MBTPS1 activity). Cells were also treated with forskolin, to induce syncytialisation, or vehicle and incubated for 48h before collection of cells and supernatants. Syncytialisation was assessed by measuring hCG secretion (by ELISA) and E-cadherin protein levels (by immunoblot and immunocytochemistry). Cellular invasion was measured using the xCELLigence real-time cell analysis system and secreted sATP6AP2 levels measured by ELISA. Forskolin successfully induced syncytialisation and significantly increased both BeWo choriocarcinoma cell invasion (P<0.0001) and sATP6AP2 levels (P=0.02). Treatment with ATP6AP2 siRNA significantly inhibited syncytialisation (decreased hCG secretion (P=0.005), the percent of nuclei in syncytia (P=0.05)), forskolin-induced invasion (P=0.046) and sATP6AP2 levels (P<0.0001). FURIN siRNA and DEC-RVKR-CMK significantly decreased sATP6AP2 levels (both P<0.0001). In conclusion, ATP6AP2 is important for syncytialisation of choriocarcinoma cells and thereby limits choriocarcinoma cell invasion. We postulate that sATP6AP2 could be used as a biomarker measuring the invasive potential of choriocarcinomas. Additionally, we confirmed that FURIN, not MBTPS1, cleaves sATP6AP2 in BeWo cells, but other proteases (inhibited by DEC-RVKR-CMK) may also be involved.

scholarly journals iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines

2017 ◽  
Vol 14 (3) ◽  
pp. 1866-1870 ◽  
Author(s):  
Leyla Türker Şener ◽  
Gürcan Albeni̇z ◽  
Bi̇rcan Di̇nç ◽  
Işil Albeni̇z
Keyword(s):  
Real Time ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Cell Analysis ◽  
Analysis System

scholarly journals Real-time live-cell analysis system for screening single tumor cell clones and analyzing their colony-forming ability

2017 ◽  
Vol 25 (10) ◽  
pp. 881
Author(s):  
Chang-Zheng Liu ◽  
Xiao-Lei Jiao ◽  
Dun-Qin Gao ◽  
Long-Bin Xing ◽  
Hui Liu ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Real Time ◽  
Live Cell ◽  
Cell Analysis ◽  
Cell Clones ◽  
Analysis System ◽  
Single Tumor Cell ◽  
Single Tumor ◽  
Live Cell Analysis

Real-time LOC-based morphological cell analysis system using high-speed vision

2014 ◽  
Author(s):  
Qingyi Gu ◽  
Tadayoshi Aoyama ◽  
Takeshi Takaki ◽  
Idaku Ishii ◽  
Ayumi Takemoto ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
Cell Analysis ◽  
Analysis System

scholarly journals Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3280 ◽  
Author(s):  
Guojun Yan ◽  
Qian Du ◽  
Xuchao Wei ◽  
Jackelyn Miozzi ◽  
Chen Kang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Herbal Medicine ◽  
Real Time ◽  
Cell Analysis ◽  
Label Free ◽  
Technology Application ◽  
Chinese Herbal ◽  
Analysis System ◽  
Free Dynamic ◽  
Application Prospects

Objective: We summarized the progress of the xCELLigence real-time cell analysis (RTCA) technology application in recent years for the sake of enriching and developing the application of RTCA in the field of Chinese medicine. Background: The RTCA system is an established electronic cellular biosensor. This system uses micro-electronic biosensor technology that is confirmed for real-time, label-free, dynamic and non-offensive monitoring of cell viability, migration, growth, spreading, and proliferation. Methods: We summarized the relevant experiments and literature of RTCA technology from the principles, characteristics, applications, especially from the latest application progress. Results and conclusion: RTCA is attracting more and more attention. Now it plays an important role in drug screening, toxicology, Chinese herbal medicine and so on. It has wide application prospects in the area of modern pharmaceutical evaluation and analysis.

scholarly journals Cytotoxicity of bracket identification dyes

2018 ◽  
Vol 89 (3) ◽  
pp. 426-431
Author(s):  
Fatih Celebi ◽  
Ahmet Altun ◽  
Ali Altug Bicakci
Keyword(s):  
Statistical Analysis ◽  
Real Time ◽  
Analysis Of Variance ◽  
Alternative Methods ◽  
Cell Analysis ◽  
Fixed Appliances ◽  
Analysis System ◽  
Post Hoc ◽  
Different Levels

ABSTRACT Objectives: To investigate the potential cytotoxicity of the bracket identification dyes commonly used in orthodontic fixed appliances. Materials and Methods: Six bracket brands representing the market in various aspects were selected. Ten sets of each bracket brand were acquired, and the identification dyes on them were scraped. They were tested for cytotoxicity at three different levels of concentration (2.5 mg/mL, 5 mg/mL, and 10 mg/mL), with the aid of a real-time cell analysis system. The results were compared within and between the groups. One-way analysis of variance and Tukey's post hoc test were used for statistical analysis. Results: None of the six investigated dyes displayed cytotoxicity at the 2.5 mg/mL concentration. Of the investigated brands, three at 5 mg/mL and four at 10 mg/mL displayed cytotoxicity. Conclusions: Some of the identification dyes in this study did display cytotoxicity at the higher concentrations tested. Alternative methods for bracket identification should be considered.

Expression Level of MiRNA-126 in Serum Exosomes of Allergic Asthma Patients and Lung Tissues of Asthmatic Mice

2019 ◽  
Vol 20 (10) ◽  
pp. 799-803 ◽  
Author(s):  
Meizhen Zhao ◽  
Yu-Pei Li ◽  
Xiao-Rui Geng ◽  
Miao Zhao ◽  
Shi-Bo Ma ◽  
...  
Keyword(s):  
Allergic Asthma ◽  
Real Time ◽  
Real Time Pcr ◽  
Lavage Fluid ◽  
Asthmatic Patients ◽  
Protein Levels ◽  
Sige Level ◽  
Bronchial Lumen ◽  
Asthma Patients ◽  
Serum Exosomes

Background: To investigate MiRNA-126 amounts in serum exosomes from allergic asthma patients as well as lung tissues of asthmatic mice, evaluating the expression of its target gene DNMT1 in mouse specimens. Methods: MiRNA-126 amounts in serum exosomes from asthmatic patients were detected by real-time PCR. The mouse model of allergic asthma was established by OVA-sensitization, and allergic symptoms were recorded; serum IL-4 and sIgE level evaluation (ELISA), broncho alveolar lavage fluid (BALF) cell count and H&E staining were performed to assess airway inflammation. MiRNA-126 and DNMT1 levels in the lung of asthmatic and control mice were detected by real-time PCR; DNMT1 protein levels were detected by immunoblot. Results: MiRNA-126 amounts in peripheral blood exosomes from patients with allergic asthma were significantly higher than that of healthy volunteers (P<0.05). The frequencies of scratching of both sides of the nose and sneezing were elevated within 10 min of excitation in asthmatic rats compared with controls. Meanwhile, OVA-sIgE and IL-4 levels were significantly higher in asthmatic animals than controls (P<0.05). In the asthma group, narrowed bronchial lumen and thickened wall were observed, and bronchial and peripheral vessels showed overt inflammatory cell infiltration. Eosinophil, neutrophil and mast cell amounts in the BALF of asthmatic mice were significantly higher than control values. Furthermore, lung miRNA-126 expression in asthmatic mice was significantly higher than that of controls. Finally, DNMT1 mRNA and protein levels were significantly lower in asthmatic animals compared with controls (P < 0.01). Conclusion: MiRNA-126 is highly expressed in serum exosomes from allergic asthma patients and lung tissues of asthmatic mice, suggesting that it may be involved in the pathogenesis of bronchial asthma.

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