scholarly journals Reduction of body-weight gain enhances in vitro embryo production in overfed superovulated dairy heifers

Reproduction ◽  
2006 ◽  
Vol 131 (4) ◽  
pp. 783-794 ◽  
Author(s):  
S Freret ◽  
B Grimard ◽  
A A Ponter ◽  
C Joly ◽  
C Ponsart ◽  
...  

The aim of our study was to test whether a reduction in dietary intake could improve in vitro embryo production in superovulated overfed dairy heifers. Cumulus–oocyte complexes of 16 Prim’ Holstein heifers (14 ± 1 months old) were collected by ovum pick-up (OPU), every 2 weeks following superovulation treatment with 250 μg FSH, before being matured and fertilized in vitro. Embryos were cultured in Synthetic Oviduct Fluid medium for 7 days. Heifers were fed with hay, soybean meal, barley, minerals and vitamins. From OPU 1 to 4 (period 1), all heifers received individually for 8 weeks a diet formulated for a 1000 g/day live-weight gain. From OPU 5 to 8 (period 2), the heifers were allocated to one of two diets (1000 or 600 g/day) for 8 weeks. Heifers’ growth rates were monitored and plasma concentrations of metabolites, metabolic and reproductive hormones were measured each week. Mean live-weight gain observed during period 1 was 950 ± 80 g/day (n = 16). In period 2 it was 730 ± 70 (n = 8) and 1300 ± 70 g/day (n = 8) for restricted and overfed groups respectively. When comparing period 1 and period 2 within groups, significant differences were found. In the restricted group, a higher blastocyst rate, greater proportions of grade 1–3 and grade 1 embryos, associated with higher estradiol at OPU and lower glucose and β-hydroxybutyrate, were observed in period 2 compared with period 1. Moreover, after 6 weeks of dietary restriction (OPU 7), numbers of day 7 total embryos, blastocysts and grade 1–3 embryos had significantly increased. On the contrary, in the overfed group, we observed more <8 mm follicles 2 days before superovulation treatment, higher insulin and IGF-I and lower nonesterified fatty acids in period 2 compared with period 1 (no significant difference between periods for embryo production). After 6 weeks of 1300 g/day live-weight gain (OPU 7), embryo production began to decrease. Whatever the group, oocyte collection did not differ between period 1 and 2. These data suggest that following a period of overfeeding, a short-term dietary intake restriction (6 weeks in our study) may improve blastocyst production and embryo quality when they are low. However, nutritional recommendations aiming to optimize both follicular growth and embryonic development may be different.

1997 ◽  
Vol 64 (3) ◽  
pp. 541-546 ◽  
Author(s):  
A. J. Hanlon ◽  
S. M. Rhind ◽  
H. W. Reid ◽  
C. Burrells ◽  
A. B. Lawrence

AbstractAt weaning at 3 months of age (week 1), 30 red deer hind calves were housed in six groups of five animals at a stocking density of 1·5 m2 per head and maintained in these groups for 4 weeks. At the start of week 5, all calves were immunized with ovalbumin (OVA). Fifteen calves, from three groups, selected at random, were transferred to individual pens which restricted visual and tactile contact with others (ISO) while the remaining animals were kept in their groups (GP). The behaviour, food intake, live-weight gain, antibody and lymphocyte responses in vitro to OVA and lymphocyte responses in vitro to the non-specific mitogen, concanavalin A (ConA), of all calves were assessed in each of weeks 5 to 9. Isolated calves had a lower mean live-weight gain than GP calves (P < 0·001), although there were no differences in food intake. Significantly more time was spent lying (P < 0·001) but less time feeding (P < 0·05) and self-grooming (P < 0·001) by ISO than by GP calves. There was no significant difference between ISO and GP calves in the cortisol response to an ACTH challenge test (10 i.u.) at week 11. Lymphocyte responses and antibody titres to OVA were lower in GP than in ISO calves at weeks 7 (P < 0·05) and 8 (P < 0·05), respectively. In contrast, GP calves had greater lymphocyte responses to the non-specific mitogen, ConA, in weeks 7 (P < 0·05) and 10 (P < 0·001) but not in week 9 compared with ISO calves. Differences in lymphocyte stimulation were attributed to the non-specific mitogenic nature of ConA. Factors such as agonistic interactions evident in group housing may have compromised the antibody and lymphocyte responses to OVA by GP calves but conversely the lack of social contact may have also suppressed behavioural activity in ISO calves.


1979 ◽  
Vol 28 (1) ◽  
pp. 41-47 ◽  
Author(s):  
K. N. Cook ◽  
Jennifer M. Newton

ABSTRACTThe trial compared 50 purebred Canadian Holstein and 46 British Friesian steers. The cattle were reared on a grass/cereal system designed to achieve an overall target live-weight gain of 0·82 kg per day from weaning to slaughter at 18 months of age. The cattle, born i n September 1973, grazed during the summer of 1974 and were slaughtered out of yards from January to July 1975. Equal numbers of each breed were allocated at random to three slaughter weights (477, 500 and 523 kg live weight). The Meat and Livestock Commission measured and classified all carcasses. In addition, a sample was cut according to commercial specifications. There were no significant differences in slaughter age or live-weight gain between the breeds. A significant difference (P < 0·05) in killing-out percentage was found in favour of the British Friesian as was a small (4%) but highly significant difference (P < 0·001) in carcass gain.The Canadian Holstein carcasses were considerably longer (P < 0·001) with less eye-muscle area (P < 0·001). The British Friesian carcasses contained 0·7% more saleable meat (P < 0·001) and 1% less bone (P < 0·01). There were no significant differences in fat trim. There were large differences in live appearance and carcass classifications, the British Friesians being of superior beef conformation.


1997 ◽  
Vol 55 (1-2) ◽  
pp. 11-19 ◽  
Author(s):  
Lisbeth Mogensen ◽  
Christian C. Krohn ◽  
Jan Tind Sørensen ◽  
Jens Hindhede ◽  
Lone Harder Nielsen

1978 ◽  
Vol 27 (2) ◽  
pp. 161-169 ◽  
Author(s):  
N. H. Cuthbert ◽  
W. S. Thickett ◽  
P. N. Wilson ◽  
T. Brigstocke

ABSTRACTSixty-four British Friesian castrated male cattle (steers) with an average starting weight of 292 kg and an average slaughter weight of 460 kg were used to compare compound diets containing 0, 10, 20 or 30% NaOH-treated straw. A basic diet of 1·8 kg hay, 1·8 kg barley straw and sugar beet pulp, on a scale rising from 1·4 to 2·8 kg according to live weight, was given throughout the trial. Compound diets were individually offered on a scale starting at 3·8 kg rising to 5·8 kg with an average intake of 4·9 kg/day. Treatments did not differ significantly in respect of live-weight gain, which ranged from 0·94 kg/day with the control to 0·97 kg/day with the 30% NaOH-treated straw compound. The grading of the animals, based on the Meat and Livestock Classification scheme, also showed no significant difference.A second trial with a further 64 British Friesian steers tested inclusion levels of 0, 30,45 and 60% treated straw in a compound diet. At the start of the trial, the cattle averaged 328 kg live weight and were slaughtered from 450 kg live weight. Animals were yarded in store condition and were given a combination of grass silage and compound diets according to a scale, based on live weight and a predicted performance of 1 kg live-weight gain per day. There were no significant differences between treatments in live-weight gain, which ranged from 0·97 kg on the control to 1·02 kg/day on the 45% NaOH-treated straw diet. There was some refusal on the 60% NaOH-treated straw diet in respect of twice daily allocations, with a few cattle on occasions not being able to consume the allowance in the 30 min provided. However, each feed allocation was always consumed before the next meal. There were no significant differences between treatments in respect of fat class, but cattle on the 45% treated-straw diet had statistically superior conformation compared with the control.


2016 ◽  
Vol 19 (1-2) ◽  
pp. 18-23
Author(s):  
H Khatun ◽  
MN Islam ◽  
AA Bhuyan ◽  
MN Hasan ◽  
MS Islam

A total of 198 straight run day old ducklings of Bangladesh Livestock Research Institute (BLRI) developed native duck (named as Rupali and Nageswary) and local native duck genotypes were distributed among nine farmers of low laying areas of Kalihati Upazilla of Tangail district. Rupali and Nageswary ducklings were obtained from existing stock of BLRI and local native ducklings were collected from local farmers of the study area. After 12 weeks of age each farmers retained 10 female and 2 male ducks and sold away remaining ducklings. Farmers’ were instructed to supply 50g of balanced feed to their ducks in the morning and evening. Ducks were allowed to scavenge in the beel throughout the day. Data on: growth, feed intake, age at onset of lay, egg production rate, egg weight, mortality rate were recorded and cost-benefit was calculated. There found no significant difference on growth parameters, live weight gain, age at first laying or age at peak egg production among the duck genotypes. In the study highest live weight gain was found in Nageswary (1090 g) followed by Rupali (1058 g) and local native (912 g) duck at 8 weeks of age. Egg production rate of local native ducks (37.21%) was found significantly lower (P<0.001) compared to Rupali (50.67 %) and Nageswary (55.40 %). The mortality rate in local duck was significantly higher (P<0.05) compared to Rupali and Nageswary ducks. Rupali ducks produced heavier eggs (66.86g) followed by local native (62.20g) and Nageswary (57.22g) which differed significantly (P<0.05). Rupali ducks laid eggs of thicker shell (0.61mm) compared to other duck genotypes under study which was also varied statistically (P<0.05). Cost benefit analysis shows that earning both from Nageswary and Rupali was much higher than local native ducks. Higher egg production rate of Nageswary and Rupali duck has contributed for higher return. It is concluded that rearing Rupali or Nageswary ducks in the low laying rural areas with scavenging and supplementary feeding facility is more profitable than rearing local native ducks.Bangladesh J. of Livestock Res. 19(1-2): 18-23, Jan-Dec 2012


1981 ◽  
Vol 32 (2) ◽  
pp. 149-153 ◽  
Author(s):  
C. Thomas ◽  
B. G. Gibbs ◽  
J. C. Tayler

ABSTRACT1. A primary growth of perennial ryegrass was cut on 8 to 11 May (early) or on 12 June (late) for comparison with the primary growth of a tetraploid red clover, which was cut either on 1 or 2 June (early) or on 28 June (late). The crops were ensiled, after wilting for about 4h, with the addition of formic acid at 2·21/t fresh crop. The silages were given ad libitum alone or with rolled barley at 11·5 g dry matter per kg live weight to 40 British Friesian steers initially 3 months old and 108 kg live weight.2. On average there was no significant difference in digestibility between perennial ryegrass and red clover. However, the rate of decline in digestibility with time was greater with perennial ryegrass than with red clover.3. Calves given silage of red clover as the sole feed ate more dry matter and grew faster than calves given grass silage (P <0·001) but supplementation with barley reduced the intake of red clover silage to a greater extent than that of perennial ryegrass silage. Barley supplementation increased live-weight gain of calves given perennial ryegrass from 0·32 to 0·83 kg/day and that of calves given red clover from 0·63 to 0·99 kg/day (interaction P <0·05). The results did not indicate a higher net efficiency of utilization of the legume. Date of cut had no significant effect on dry-matter intake but earlier cutting resulted in an increase in live-weight gain from a mean of 0·61 to 0·77 kg/day (P <0·001).4. The results show that high rates of live-weight gain (0·74 kg/day) can be achieved by calves given silage of red clover and that earlier cutting of herbage for silage does not always result in higher intakes of dry matter. Supplementation of silages with barley can reduce markedly the difference in intake and live-weight gain apparent when the silages are given as sole feeds.


1974 ◽  
Vol 19 (2) ◽  
pp. 193-200 ◽  
Author(s):  
R. M. Tetlow ◽  
R. J. Wilkins

SUMMARYCobs and pellets which differed in particle size (modulus of fineness, MF) were prepared from dried perennial ryegrass and tall fescue and given to sheep. The MF values of the feeds were 2·45,1·09,0·81 and 0·74 for perennial ryegrass and 2·28,0·84,0·67 and 0·57 for tall fescue. Organic matter digestibility was similar for the two grass species when measured in vivo, but when measured in vitro values for perennial ryegrass were about 3 units higher than those for tall fescue. Voluntary intake and live-weight gain were higher for sheep given tall fescue, the difference in live-weight gain being just significant at P < 0·05. The response to change in particle size was similar for the two grass species. Intake was lowest and digestibility highest for the feeds with the highest MF, but differences between the three finely-ground forms of each species were not significant. There was a tendency for the intake of digestible organic matter to be lower for the feeds with lowest MF than for those with intermediate values for MF. The rate of passage of undigested particles was slower for cobs with the highest MF than for the other feeds, which did not differ significantly one from another.


1981 ◽  
Vol 97 (3) ◽  
pp. 669-676 ◽  
Author(s):  
S. E. Watson ◽  
P. C. Whiteman

SUMMARYA grazing study, comparing a naturalized and a sown pasture at three stocking rates, was conducted under a uniform stand of 65-year-old coconuts on a fertile soil in the Russell Islands. In a plot trial on this site there was no significant response to application of up to 400 kg N/ha/year over 2 years to Brachiaria decumbens. Average daily transmission of photosynthetically active radiation through the coconut canopy was 60% of full sunlight. The sown pasture consisted initially of para (Brachiaria mutica), signal (B. decumbens) and koronivia (B. humidicola) with the legumes Centrosema pubescens, Pueraria phaseoloides and Stylosanthes guianensis. The main species in the naturalized pasture were Axonopus compressus, Mimosa pudica, C. pubescens and Colopogonium mucunoides. Pastures were set stocked in two replicates over 3 years at 1·5, 2·5 and 3·5 animals/ha. There was no significant difference in live-weight gain between pastures in any year. Live-weight gain per head declined linearly with increasing stocking rate. Highest live-weight gain was 437 kg/ha/year in the 1st year at 3·5 animals/ha. The planted grasses declined from 60% at the start of grazing to 6% 8 months later. A. compressus increased from 2 to 24% over 3 years in the sown pasture, and from 12 to 34% of yield in the natural pasture. M. pudica increased from 27 to 44% in the sown, and remained approximately constant at 37% in the natural pasture. G. pubescens increased at 2·5 animals/ha, but was replaced by P. phaseoloides at 1·5 animals/ha and by A. compressus and M. pudica at 3·5 animals/ha. There were no significant effects of pasture treatments or stocking rate on copra yields. Where there is a cover of naturalized grasses and legumes under coconuts, cultivation and planting of exotic species cannot be recommended. Major improvement will come from thorough weed control and maintaining stocking rates between 1·5 and 2·5 animals/ha.


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