Transcriptomic Analysis Identifies Candidate Genes Related to Intramuscular Fat Deposition and Fatty Acid Composition in the Breast Muscle of Squabs (Columba)

Intramuscular fat (IMF) and fatty acid composition are characteristics that are used as important indicators of evaluating high quality pork and contribute to the economic benefits of the pig farming industry. In this study, quantitative trait loci (QTL) fine mapping of chromosome 12 was performed in a population of F2 intercross between Yorkshire (YS) and Korean native pigs (KNPs) by adopting combined linkage and linkage disequilibrium method using high-density SNP chips. QTLs for IMF (H3GA0034813 to H3GA0034965) and oleic acid (C18:1) (ASGA0054380 to ALGA0066299) were located at 120 cM (54.112–57.610 kb) and 85 cM (36.097–38.601 kb), respectively, within chromosome 12 (Sscrofa11.1 genomic reference). In addition, 31 candidate genes present within the IMF QTL region and 28 candidate genes existing within C18:1 QTL region were chosen. In order to understand the function of these candidate genes at the molecular level, these candidate genes were functionally categorized by studying gene ontology and analyzing network and pathway. Among the 59 candidate genes within the region of IMF QTL and C18:1 QTL, five (MYH1, MYH2, MYH4, ACACA, and RPS6KB1) directly interacting candidate genes were found. Furthermore, the RPS6KB1 gene was assumed to be an important candidate gene that is involved in leptin and insulin signaling pathway and participates in controlling adipogenic differentiation, fat deposition, and fatty acid composition, which is related to obesity of pigs.

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Effect of a high dose of CLA in finishing pig diets on fat deposition and fatty acid composition in intramuscular fat and other fat depots

Meat Science ◽

10.1016/j.meatsci.2012.10.005 ◽

2013 ◽

Vol 93 (3) ◽

pp. 517-524 ◽

Cited By ~ 16

Author(s):

Núria Tous ◽

Rosil Lizardo ◽

Borja Vilà ◽

Marina Gispert ◽

Maria Font-i-Furnols ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Intramuscular Fat ◽

Fat Deposition ◽

High Dose ◽

Fat Depots ◽

Finishing Pig

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Comparative Transcriptomic Analysis Reveals Diverse Expression Pattern Underlying Fatty Acid Composition among Different Beef Cuts

Foods ◽

10.3390/foods11010117 ◽

2022 ◽

Vol 11 (1) ◽

pp. 117

Author(s):

Tianliu Zhang ◽

Qunhao Niu ◽

Tianzhen Wang ◽

Xu Zheng ◽

Haipeng Li ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Candidate Genes ◽

Acid Composition ◽

Genetic Selection ◽

Transcriptomic Analysis ◽

Functional Enrichment ◽

Ampk Signaling ◽

Gene Network Analysis ◽

Comparative Transcriptomic Analysis

Beef is an important dietary source of quality animal proteins and amino acids in human nutrition. The fatty acid composition is one of the indispensable indicators affecting nutritional value of beef. However, a comprehensive understanding of the expression changes underlying fatty acid composition in representative beef cuts is needed in cattle. This study aimed to characterize the dynamics of fatty acid composition using comparative transcriptomic analysis in five different type of beef cuts. We identified 7545 differentially expressed genes (DEGs) among 10 pair-wise comparisons. Co-expression gene network analysis identified two modules, which were significantly correlated with 2 and 20 fatty acid composition, respectively. We also identified 38 candidate genes, and functional enrichment showed that these genes were involved in fatty acid biosynthetic process and degradation, PPAR, and AMPK signaling pathway. Moreover, we observed a cluster of DEGs (e.g., SCD, LPL, FABP3, and PPARD) which were involved in the regulation of lipid metabolism and adipocyte differentiation. Our results provide some valuable insights into understanding the transcriptome regulation of candidate genes on fatty acid composition of beef cuts, and our findings may facilitate the designs of genetic selection program for beneficial fatty acid composition in beef cattle.

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Association of osteopontin gene with intramuscular fat content and fatty acid composition traits in pigs

Veterinary Integrative Sciences ◽

10.12982/vis.2021.018 ◽

2020 ◽

Vol 19 (2) ◽

pp. 197-208

Author(s):

Nanthana Pothakam ◽

◽

Worrarak Norseeda ◽

Guisheng Liu ◽

Tawatchai Teltathum ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Intramuscular Fat ◽

Fat Deposition ◽

Fa Composition ◽

Muscle Tissues ◽

Ether Extraction ◽

Pcr Rflp ◽

Imf Content

Osteopontin (OPN) is a secreted phosphoprotein that is involved in the development of skeletal muscle and fat deposition. The objectives of this study were to identify the polymorphism of the OPN gene and to analyze the association of the OPN gene with intramuscular fat (IMF) content and fatty acid (FA) composition in pigs. Longissimus thoracis (LT) muscle samples taken from the 10-11th rib were collected from a total of 328 Duroc pigs. Genomic DNA samples were extracted from LT muscle tissues using the phenol-chloroform method. IMF content was measured using the ether extraction method and FA composition was measured by gas chromatography. The porcine OPN polymorphisms were identified by DNA sequencing and were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The association analysis of the OPN gene with IMF and FA composition traits was performed using a general linear model (GLM). Two polymorphic sites (OPN g.2442-2471indel and g.3836A>G) were found in the 5´-flanking region and intron 1 of the porcine OPN gene. The OPN g.2442-2471indel polymorphism was found to be significantly associated with IMF content and ω3 FA levels (P<0.05). Moreover, OPN g.3836A>G polymorphism was significantly associated with the linolenic acid levels in the muscles of pigs (P<0.05). The results of this study indicate that the OPN gene is important to IMF content, as well as linolenic and ω3 FA levels in pigs, and could be used as a candidate gene to improve fat deposition and fatty acid composition in the muscles of pigs.

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Research Article Candidate genes expression affect intramuscular fat content and fatty acid composition in Tan sheep

Genetics and Molecular Research ◽

10.4238/gmr18550 ◽

2020 ◽

Vol 19 (4) ◽

Author(s):

X. Xu ◽

W. Chen ◽

S. Yu ◽

S. Fan ◽

W. Ma

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Candidate Genes ◽

Acid Composition ◽

Intramuscular Fat ◽

Fat Content ◽

Research Article ◽

Genes Expression ◽

Tan Sheep ◽

Intramuscular Fat Content

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Effect of slaughter season on fatty acid composition, conjugated linoleic acid isomers and nutritional value of intramuscular fat in Barrosã-PDO veal

Meat Science ◽

10.1016/j.meatsci.2006.06.013 ◽

2007 ◽

Vol 75 (1) ◽

pp. 44-52 ◽

Cited By ~ 41

Author(s):

Cristina M.M. Alfaia ◽

Matilde L.F. Castro ◽

Susana I.V. Martins ◽

Ana P.V. Portugal ◽

Susana P.A. Alves ◽

...

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Linoleic Acid ◽

Conjugated Linoleic Acid ◽

Acid Composition ◽

Nutritional Value ◽

Intramuscular Fat

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Fatty acid composition of subcutaneous and intramuscular adipose tissue in east Balkan pigs

Biotechnology in Animal Husbandry ◽

10.2298/bah1504543p ◽

2015 ◽

Vol 31 (4) ◽

pp. 543-550 ◽

Cited By ~ 2

Author(s):

T. Popova ◽

J. Nakev ◽

Y. Marchev

Keyword(s):

Fatty Acids ◽

Adipose Tissue ◽

Fatty Acid Composition ◽

Fatty Acid ◽

Polyunsaturated Fatty Acids ◽

Acid Composition ◽

Subcutaneous Fat ◽

Intramuscular Fat ◽

Longissimus Dorsi ◽

Intramuscular Adipose Tissue

The aim of this study was to provide information on the fatty acid profile of different adipose depots - subcutaneous (upper and inner backfat layers) and intramuscular (m. Longissimus dorsi) in East Balkan pigs. The animals were reared in free-range conditions and slaughtered at an average live weight of 107?1.65kg. The results of the study showed that the various adipose tissues in pigs have different lipid metabolism and hence differ in their fatty acid composition. Intramuscular fat had significantly higher content of the saturated C16:0 and C18:0 (P<0.001), as well as the C16:1 (P<0.001) than the subcutaneous fat. In regards to the content of the polyunsaturated fatty acids, the latter displayed considerably higher content of both C18:2 and C18:3 (P<0.001) in comparison to the intramuscular fat in m. Longissimus dorsi. The differences between the subcutaneous and intramuscular adipose tissue in the individual fatty acids determined the similar trend of change in the total content of saturated and polyunsaturated fatty acids. Significant differences between the backfat layers were detected for C16:1, C18:0 and C18:3 (P<0.001). Stearic acid (C18:0) displayed higher content of the inner, while both C16:1 and C18:3 had higher proportion in the outer backfat layer in the East Balkan pigs. Except for C20:2, the long chain polyunsaturated n-6 and n-3 fatty acids had significantly higher proportions in the intramuscular fat, however no differences were determined between the two backfat layers.

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