scholarly journals Effect of two cytokinins and a growth inhibitor on the in vitro tuberization of two genotypes of Solanum tuberosum L. cvs. Atlantic and Alpha

Uniciencia ◽  
2019 ◽  
Vol 33 (2) ◽  
pp. 1-12
Author(s):  
José Antonio García-García ◽  
José Bernal Azofeifa-Bolaños ◽  
Frank Solano-Campos ◽  
Rafael Orozco-Rodríguez
Keyword(s):  
Culture Medium ◽  
Seed Quality ◽  
Solanum Tuberosum L ◽  
Growth Inhibitor ◽  
Control Treatment ◽  
In Vitro Tuberization ◽  
Hormonal Imbalance ◽  
Basal Culture Medium ◽  
Significant Increment

This investigation was carried out to evaluate the effect of two cytokinins: 6-benzilaminopurine (BAP) (6.5 mg l-1) and kinetin (K) (2.5 mg l-1), as well as the growth inhibitor abscisic acid (ABA) (1.0 mg l-1) on the in vitro tuberization capacity of two potato varieties: Atlantic and Alpha. The basal culture medium MS (1962) was used as a control. The responses were different between varieties. In cv. Atlantic, the analysis of the number (NM), weight (WM) and diameter (DM) of microtubers indicated that the addition of growth regulators did not affect induction and development of microtubers. However, when BAP was used, a non-significant increment of 41 % was observed in the number of the microtubers compared to the control treatment, from 2.6 to 4.4. The addition of cytokinins and ABA to the medium did not have a significant impact on the development of microtubers. In cv. Alpha the cytokinins used without ABA increased the number of microtubers, which were larger and heavier than those of the control treatment. In this variety, ABA significantly reduced the values of the NM, WM and DM variables. The exogenous action of cytokinins in the culture medium is likely to have caused an endogenous hormonal imbalance in the Atlantic and Alpha genotypes which interfered with their innate microtuberization ability, a result that was even more evident for cv. Alpha, which showed the need to continue optimizing protocols of genotype-specific systems in potato tissue culture to increase yield and seed quality.

scholarly journals Temporary immersion biorreators: efficient technique for the propagation of the ‘Pircinque’ strawberry

2019 ◽  
Vol 41 (1) ◽  
Author(s):  
Samila Silva Camargo ◽  
Leo Rufato ◽  
Maicon Magro ◽  
André Luiz Kulkamp de Souza
Keyword(s):  
Liquid Medium ◽  
Culture Medium ◽  
Culture Media ◽  
Time Factors ◽  
Efficient Technique ◽  
Control Treatment ◽  
Solid Culture ◽  
Temporary Immersion ◽  
Solid Culture Medium

Abstract The in vitro propagation technique via temporary immersion bioreactors is a tool that, through the culture in a liquid medium, allows an increase in the efficiency of seedling production. Several researches with the strawberry crop have shown greater efficiency of the system compared to the conventional process of micropropagation in solid medium. In this sense, the objective herein was to establish a protocol of multiplication and rooting of the ‘Pircinque’ strawberry, in temporary immersion bioreactors. Two distinct and independent studies were carried out, characterized by the multiplication and rooting stages of strawberry explants, newly introduced and registered in Brazil. Two culture media (MS and KNOP) were studied and, as a control treatment, the growth of the explants in solid culture medium was evaluated with the addition of 5 g L-1 of agar. Different immersion times of the culture medium were explored: five or eight times a day, for 15 minutes. The study was composed of the culture medium and immersion time factors, as well as the control (solid) treatment. It was verified that the use of temporary immersion bioreactors system is an efficient technique for the multiplication and rooting of explants of strawberry cv. Pircinque, when compared to the conventional method of micropropagation with the use of solid culture medium, making it possible to optimize the production of seedlings in biofactories. The MS liquid medium, in contact with explants of ‘Pircinque’ strawberry five times a day, increased the growth of the aerial part and the root system.

scholarly journals Exploitation of somaclonal variability for the search of saline-tolerant potato (Solanum Tuberosum L.)

1970 ◽  
pp. 08-12
Author(s):  
BENDIF Hamdi ◽  
LAZALI Mohammed ◽  
MESSAOUDI Noura, GHADBANE Mouloud ◽  
KHENIFI Mohamed-Amine, KADRI Farida, BOUDJENIBA messaoud
Keyword(s):  
Salt Stress ◽  
Solanum Tuberosum ◽  
Culture Medium ◽  
Solanum Tuberosum L ◽  
Sensitivity Coefficient ◽  
Callus Growth ◽  
Average Growth ◽  
Somaclonal Variability ◽  
Potato Callus

The present investigation was conducted with an aim to study the effect of salt stress (NaCl) on in vitro potato callus growth and the potential for exploiting this technique in improving salinity tolerance in potato. The results in the four varieties in the culture medium showed a decrease in all studied parameters like callus growth, relative average growth, water content and salinity sensitivity coefficient upon increasing the salinity of the medium in calluses. It has also been observed that the type of callus has a large effect on the above-mentioned parameters in which better callus growth adapted to 150 mmol NaCl is obtained than unsuitable calli in the same culture medium.

scholarly journals In vitro organogenesis in some citrus species

2011 ◽  
Vol 33 (2) ◽  
pp. 526-531 ◽  
Author(s):  
Evandro Henrique Schinor ◽  
Fernando Alves de Azevedo ◽  
Francisco de Assis Alves Mourão Filho ◽  
Beatriz Madalena Januzzi Mendes
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Adventitious Shoots ◽  
Medium Composition ◽  
Naphthaleneacetic Acid ◽  
In Vitro Organogenesis ◽  
Basal Culture Medium ◽  
Medium Supplementation ◽  
Media Supplementation

In vitro organogenesis of Citrus was studied for the genotypes Citrus sinensis cv. 'Natal', C. limonia, C. volkameriana, and C. aurantium, with the use of epicotyl segments-derived explants, cultured in MT salts and vitamins medium supplemented with different concentrations of 6-benzylaminopurine (BAP - 0.0; 0.5; 1.0; 1.5 or 2.0 mg L-1). For the recalcitrant genotypes C. limonia and C. aurantium the in vitro organogenesis was also studied with internodal segments-derived explants, cultured in MT salts and vitamins medium supplemented with 0; 0.5; 1.0; 2.0, or 4.0 mg L-1 of BAP. The efficiency of culture medium supplementation with the combination of BAP (0.0; 1.0, or 2.0 mg L-1) and NAA (1-naphthaleneacetic acid - 0.0; 0.3, or 0.5 mg L-1) in the development of adventitious shoots was evaluated for C. aurantium. Culture medium supplementation with BAP is not essential for the adventitious shoots development in the four genotypes studied when epicotyl segments-derived explants are used. In general, culture media supplementation with BAP decreased the percentage of responsive explants excepted for C. sinensis cv. 'Natal' and C. limonia when the concentrations of 1.5 and 2.0 mg/L were used. The presence of cytokinin, in concentrations up to 2 mg/L, stimulated the in vitro organogenesis when internodal segments-derived explants were used for C. limonia and C. aurantium. For C. aurantium no adventitious shoots developed in explants (internodal segments) cultured in basal culture medium, without BAP supplementation. Although no statistic differences could be detected, culture media supplementation with the combination of BAP and NAA favored the development of adventitious shoots in C. aurantium. The best concentration of NAA varied according to BAP concentration. The results presented herein, show that Citrus in vitro organogenesis depends on the interaction of culture medium composition, explant differentiation level, and genotype.

Hyperhydricity reversal and clonal propagation of four-wing saltbush (Atriplex canescens, Chenopodiaceae) cultivated in vitro

2008 ◽  
Vol 56 (4) ◽  
pp. 358 ◽  
Author(s):  
Isaac Reyes-Vera ◽  
Carol Potenza ◽  
Jerry Barrow
Keyword(s):  
Culture Medium ◽  
Clonal Propagation ◽  
Inorganic Nitrogen ◽  
Basal Medium ◽  
Control Treatment ◽  
Culture Vessel ◽  
Cumulative Frequency ◽  
Atriplex Canescens ◽  
Normal Morphology

In vitro propagated shoots of four-wing saltbush (Atriplex canescens, Pursh Nutt) showed severe symptoms of hyperhydricity. We show that the reversion of hyperhydric A. canescens shoots to normal shoots was significantly affected by the presence of inorganic nitrogen in the culture vessel. When the culture vessel was vented or when ammonium nitrate was deleted from Murashige and Skoog basal medium, rates of reversion were significantly higher. Although statistically significant differences were evident when comparing vented v. non-vented treatments for each medium, the modified culture medium with vented closures was consistently the best treatment, showing a total cumulative frequency of 39.7% reversion to normal morphology, compared with a total cumulative frequency of 7.1% observed in the control treatment. Resulting normal shoots also showed significant improvements in further manipulations, including rooting in vitro, transplantation to soil and survival in native sites.

scholarly journals Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 1010
Author(s):  
Eucario Mancilla-Álvarez ◽  
Juan Antonio Pérez-Sato ◽  
Rosalía Núñez-Pastrana ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Culture Medium ◽  
Solid Medium ◽  
Survival Rates ◽  
Shoot Multiplication ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Solid Culture ◽  
Solid Culture Medium ◽  
Semi Solid

Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

scholarly journals Evaluation of different antioxidants during in vitro establishment of allspice (Pimenta dioica L. Merrill): a recalcitrant species

2020 ◽  
Author(s):  
Sugey Vásquez-Hernández ◽  
Carlos Alberto Cruz-Cruz ◽  
Maricela Santiago-Santiago ◽  
Jericó Jabín Bello-Bello
Keyword(s):  
Lipid Peroxidation ◽  
Methylene Blue ◽  
Cell Wall ◽  
Antioxidant Capacity ◽  
Culture Medium ◽  
Control Treatment ◽  
Soluble Phenols ◽  
In Vitro Establishment ◽  
Pimenta Dioica

Abstract Background: Pimenta dioica L. Merrill is a tree whose fruits are used as a spice due to their culinary and therapeutic uses. Conventional propagation techniques using seeds and cuttings do not guarantee the phytosanitary quality of this crop. Therefore, the use of Plant Tissue Culture techniques are an option for in vitro establishment. The aim of this study was to evaluate the effect of different antioxidant agents (Methylene blue, L-cysteine and silver nanoparticles) added to MS (Murashige and Skoog) culture medium at different concentrations (0, 50, 100 and 200 mg L-1) during axenic establishment of buds used as explants of P. dioica. Results: The percentage of survival, oxidation and contamination was determined, as well as the content of soluble phenols, cell wall-linked phenols, antioxidant capacity and lipid peroxidation. Results showed significant differences among the different antioxidants for the evaluated variables; the highest survival occurred in the treatments with the addition of L-cysteine with percentages greater than 40 %, while the lowest survival occurred in the control treatment, 100 and 200 mg L-1 methylene blue, with 0, 3.3 and 0% survival, respectively. The highest percentage of oxidation was observed in the control treatments, 100 and 200 mg L-1 methylene blue with 96.67% oxidation, while the lowest percentages were observed in explants treated with L-cysteine, with 30% oxidation. Treatments with 100 and 200 mg L-1 AgNPs had the lowest contamination values, with 20%. Biochemical determinations showed that L-cysteine and 50 and 100 mgL-1 AgNPs resulted in an increase in the content of soluble phenols. The highest contents of cell wall-linked phenols were obtained in treatments with 200 mg L-1 methylene blue, L-cysteine, and 200 mg L-1 AgNPs. Analysis of antioxidant capacity revealed that all treatments had a reaction of scavenging / reduction mechanisms free radicals. Regarding lipid peroxidation, the highest content of malondialdehydes was observed in the control treatment and 200 mgL-1 methylene blue. Conclusion: the addition of L-cysteine to the culture medium showed a higher survival rate, decreased oxidation, greater production of phenolic compounds, increased antioxidant capacity and decreased lipid peroxidation, this amino acid being an alternative to reduce oxidation during in vitro introduction of allspice and other species that exhibit recalcitrance in vitro during establishment.

scholarly journals Evaluation of different antioxidants during in vitro establishment of allspice (Pimenta dioica L. Merrill): a recalcitrant species

2021 ◽  
Author(s):  
Sugey Vásquez Hernández ◽  
Carlos Alberto Cruz Cruz ◽  
Maricela Santiago Santiago ◽  
Jericó Jabín Bello Bello
Keyword(s):  
Methylene Blue ◽  
Culture Medium ◽  
Control Treatment ◽  
Reduction Mechanisms ◽  
Antioxidant Agents ◽  
Randomized Experimental Design ◽  
Soluble Phenols ◽  
In Vitro Establishment ◽  
Pimenta Dioica

Objective: To evaluate the effect of different antioxidant agents during in vitro establishment of allspice (Pimenta dioica L. Merrill). Design/methodology/approach: The effect of different antioxidant agents (Methylene blue, L-cysteine, and silver nanoparticles [AnNPs]) added to Murashige and Skoog culture medium at different concentrations were studied during axenic establishment of P. dioica. A completely randomized experimental design was used. All trials were performed in triplicate. The percentage of survival, oxidation, contamination was determined, the phenols content, antioxidant capacity and lipid peroxidation. Results: The highest survival occurred with the addition of L-cysteine. The lowest percentages oxidation were observed in explants treated with L-cysteine. Treatments with 100 and 200 mg L-1 AgNPs had the lowest contamination values. L-cysteine and 50 and 100 mg L-1 AgNPs resulted in an increase in the content of soluble phenols. The highest contents of cell wall-linked phenols were obtained in treatments with 200 mg L-1 methylene blue, L-cysteine, and 200 mg L-1 AgNPs. In this study, all treatments had a reaction of scavenging/reduction mechanisms free radicals. The highest content of malondialdehydes was observed in the control treatment and 200 mg L-1 methylene blue. The highest content of malondialdehydes was observed in the control treatment and 200 mg L-1 methylene blue. Limitations on study/implications: The highest percentage of oxidation was observed in the control treatments, 100 and 200 mg L-1 methylene blue, causing cell death. Findings/conclusions: The addition of L-cysteine to the culture medium is alternative to reduce oxidation during in vitro introduction of P. dioica.

scholarly journals Elicitation of Phenylpropanoids in Maqui (Aristotelia chilensis [Mol.] Stuntz) Plants Micropropagated in Photomixotrophic Temporary Immersion Bioreactors (TIBs).

2021 ◽  
Author(s):  
Giulia E Trentini ◽  
Makarena Rojas ◽  
Daniela Gajardo ◽  
Débora Alburquenque ◽  
Evelyn Villagra ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Plants ◽  
Control Treatment ◽  
Multiplication Rate ◽  
Fresh Weight ◽  
Temporary Immersion ◽  
Air Treatment ◽  
The Third ◽  
Aristotelia Chilensis

Abstract A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m− 2s− 1. The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO2 do not differ significantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air. Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not significant differences in polyphenols and anthocyanin contents were verified between the treatments of maqui in TIBs + ABA and controls. The non-significant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no significant differences by the ORAC test from day 5 to day 15 of the study.

scholarly journals Micro propagation of four Potato Cultivars Solanum tuberosum L. in vitro

2014 ◽  
Vol 8 (4) ◽  
pp. 19-24
Author(s):  
Zainab Abduljabar Hussain AL-Hussaini ◽  
Shatha Ayed Yousif ◽  
Shymaa Abdullateef Musa ◽  
Nora Sahib Abd ◽  
Tagreed Abduljabar ◽  
...  
Keyword(s):  
Solanum Tuberosum L ◽  
Control Treatment ◽  
Significant Interaction Effect ◽  
Shoot Bud ◽  
Micro Propagation ◽  
Initiation Stage ◽  
Two Stages ◽  
Number Of Branches ◽  
Plant Length

Vegetative buds excised from tubers for four varieties of potatoes namely: Arnova, Brovento, Rivira and Bureen (after breaking the dormancy phase) which sterilized with 2% Sodium hypochlorite for 10 minutes and cultured in the MS medium supplemented with 0.5 mg/l of IAA or IBA with different concentration of BA, as well as control treatment in the initiation stage, while different concentrations of BA (0.0, 0.5, 1.0, 1.5, 2 mg/l) with 0.5 IAA mg/l in multiplication stage. All cultures incubated at a temperature of 2 ± 25º C and an illumination of 1000 lux for 16 hours/ day. Data of mean number of branches shoot per plant and their length and leaves for two stages were investigated. The results showed that BA + IAA at 0.5 mg/L for each one, gave the highest initiated percentage, shoot per plant ,length, reached 75%, 1.5 shoot / bud, 2.21cm and 3.88, leaf/ bud, respectively. Results of multiplication indicated that there was a significant interaction effect of both cultivars and BA concentration on all characterizes, Based on this interaction, Arnova cultivars at 1.5 mg/L BA showed the highest mean of shoot per plant (4.0) While Rivera cultivars at 0.5 mg/L BA gave highest mean of length, leaves per plant reached 6.45cm,7.75 leaf/plant respectively.

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