Identification of bioactive compounds in Solanum incanum fruit by Thin layer Chromatography and HPTLC

2012 ◽  
Vol 2 (6) ◽  
pp. 22-25 ◽  
Author(s):  
Indhumathi.T Indhumathi.T ◽  
◽  
Dr. S. Mohandass Dr. S. Mohandass
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
Solanum Incanum ◽  
Layer Chromatography

scholarly journals Original Article BIOACTIVE COMPOUNDS FROM SPONGE SUBERITES CARNOSUS (JOHNSTON) COLLECTED FROM WEST COAST OF MUMBAI, INDIA

2021 ◽  
pp. 41-51
Author(s):  
BHADEKAR N. S ◽  
ZODAPE G. V.
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
West Coast ◽  
Acid Methyl Ester ◽  
Biological Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Ftir Analysis ◽  
Infrared Spectrometer ◽  
Layer Chromatography

Objective: Structural elucidation of bioactive compounds from marine Sponge Suberites Carnosus (Johnston) by using Thin Layer Chromatography (TLC), Gas Chromatography-Mass Spectrometry (GC-MS), Fourier Transform Infrared Spectrometer (FTIR) techniques with respect to its pharmacological and biomedical properties. Methods: The sponge Suberites carnosus (Johnston) was collected during low tides from West Coast of Mumbai. Crude extract was obtained by taking 10 gram of sponge sample in10 ml of methanol. The preparative TLC (Thin Layer Chromatography) was performed by using Toluene: Ethyl acetate: Diethylamine (7:2:1) (v/v). The isolated compounds were subjected to GC-MS and FTIR analysis. Results: From the GC-MS and FTIR analysis, total ten compounds were identified. The GC-MS spectra correlate to the mass spectra. The structures of those compounds are 6-Fluoro 2-trifluromethylbenzoic acid,2,3-dichlorophenyl ester, Eicosane 3-cyclohexyl, Phosphine imide, P,P,P,-tris (p-chlorophenyl)-N-phenyl-, Dimethylhyl hexavinyl octasilsesquioxane, Hexanoicacid, hexadecyl ester, Hexadecanoic acid,2-hydroxy1-(hydroxymethyl)ethyl ester, 9, 19-Cyclolanostan-3-ol, acetate, (3β), Tetracosane, 3-ethyl-, 11, 14-Eicosadienoic acid, methyl ester, Triacontane, 11,20-didecyl-respectively. Conclusion: The nature and biological properties of the said compounds were determined and it was found that some of them act as a skin irritant. Some of them have fatty, metabolite, and irritant property, whereas some are act as masking and perfuming agents. Some compounds are highly toxic property, whereas others have an effect on health and environmental hazard. Some are highly corrosive in nature, whereas others are Cholesterol and chemotaxonomic significance.

scholarly journals Puree and Juice of Thai Mango and Pineapple Analyzed by High-Performance Thin-Layer Chromatography Hyphenated with Effect-Directed Assays

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7683
Author(s):  
Gertrud E. Morlock ◽  
Newitchaya Wutthinithisanand ◽  
Doris Rauhut
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
High Performance ◽  
Mangifera Indica ◽  
Quality Criterion ◽  
Health Claims ◽  
Ananas Comosus ◽  
Production Chain ◽  
Layer Chromatography

The requirements for analytical tools are changing due to the global production chain, the increasing cases of adulteration, and the growing trend towards consumption of plant-based food products worldwide. The assessment of bioactivity of natural foods is currently not a quality criterion, and a paradigm shift is postulated. A non-targeted effect-directed profiling by high-performance thin-layer chromatography hyphenated with five different effect-directed assays was developed exemplarily for the puree and juice products of mango Mangifera indica L. (Anacardiaceae) and pineapple Ananas comosus (L.) Merr. (Bromeliaceae). Several bioactive compounds were detected in each sample. The additional bioactivity information obtained through effect-directed profiles improves, expands and modernizes product control. Non-target effect-directed profiling adds a new perspective to previous target analysis results that can be used not only to ensure health claims based on bioactive compounds, but also to detect unknown bioactive compounds coming from contamination or residues or changes caused by food processing.

Calophyllolide Separation from Calophyllum inophyllum Oil by Silica Gel Adsorption

2020 ◽  
Vol 988 ◽  
pp. 101-107
Author(s):  
Setiyo Gunawan ◽  
Brilian Pamungkas ◽  
Claudia Shantika Primaswari ◽  
Safrina Hapsari ◽  
Hakun Wirawasista Aparamarta
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Bioactive Compounds ◽  
Separation Method ◽  
Raw Material ◽  
Mass Ratio ◽  
Layer Chromatography ◽  
Stem Leaf

Nyamplung (Calophylluminophyllum), widely spreads in Indonesia archipelago, is known to have many advantages. It has various benefits that can be utilized from its root, stem, leaf, and seed. C inophyllum’s seed contains bioactive compounds called calophyllolide. However, the utilization of C. inphyllum’s seed is limited because it contains harmful toxins. Therefore, C. inophyllum’s seed is generally used and investigated as a raw material of biodiesel. This research aimed to find the best condition to isolate calophyllolide, to know the yield of isolated calophyllolide and to know its purity percentage from crude C. inophyllum oil. Current research on calophyllolide is to extract the substance from C. inophyllum’s shell nut without further treatment on it. In this work, calophyllolide was separated from crude C. inophyllum oil by silica gel adsorption. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze calophyllolide mixture. In this study, the best separation method (12.92% purity, 8.03% yield, and 95.02% recovery of calophyllolide) was obtained by using crude C. inophyllum to silica gel mass ratio of 1:2 (g/g).

scholarly journals Detection Progress of Selected Drugs in TLC

2014 ◽  
Vol 2014 ◽  
pp. 1-19 ◽  
Author(s):  
Alina Pyka
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
Chemical Structure ◽  
Limit Of Detection ◽  
Direct Detection ◽  
Herbal Extracts ◽  
Linearity Range ◽  
Photocatalytic Reactions ◽  
Layer Chromatography

This entry describes applications of known indicators and dyes as new visualizing reagents and various visualizing systems as well as photocatalytic reactions and bioautography method for the detection of bioactive compounds including drugs and compounds isolated from herbal extracts. Broadening index, detection index, characteristics of densitometric band, modified contrast index, limit of detection, densitometric visualizing index, and linearity range of detected compounds were used for the evaluation of visualizing effects of applied visualizing reagents. It was shown that visualizing effect depends on the chemical structure of the visualizing reagent, the structure of the substance detected, and the chromatographic adsorbent applied. The usefulness of densitometry to direct detection of some drugs was also shown. Quoted papers indicate the detection progress of selected drugs investigated by thin-layer chromatography (TLC).

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