Calophyllolide Separation from Calophyllum inophyllum Oil by Silica Gel Adsorption

2020 ◽  
Vol 988 ◽  
pp. 101-107
Author(s):  
Setiyo Gunawan ◽  
Brilian Pamungkas ◽  
Claudia Shantika Primaswari ◽  
Safrina Hapsari ◽  
Hakun Wirawasista Aparamarta
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Bioactive Compounds ◽  
Separation Method ◽  
Raw Material ◽  
Mass Ratio ◽  
Layer Chromatography ◽  
Stem Leaf

Nyamplung (Calophylluminophyllum), widely spreads in Indonesia archipelago, is known to have many advantages. It has various benefits that can be utilized from its root, stem, leaf, and seed. C inophyllum’s seed contains bioactive compounds called calophyllolide. However, the utilization of C. inphyllum’s seed is limited because it contains harmful toxins. Therefore, C. inophyllum’s seed is generally used and investigated as a raw material of biodiesel. This research aimed to find the best condition to isolate calophyllolide, to know the yield of isolated calophyllolide and to know its purity percentage from crude C. inophyllum oil. Current research on calophyllolide is to extract the substance from C. inophyllum’s shell nut without further treatment on it. In this work, calophyllolide was separated from crude C. inophyllum oil by silica gel adsorption. Each fraction obtained was tested qualitatively using Thin Layer Chromatography (TLC) and quantitatively using Gas Chromatography (GC) to analyze calophyllolide mixture. In this study, the best separation method (12.92% purity, 8.03% yield, and 95.02% recovery of calophyllolide) was obtained by using crude C. inophyllum to silica gel mass ratio of 1:2 (g/g).

Thin-layer chromatography and gas chromatography-mass spectrometry examination of shoe materials from patients with shoe dermatitis

2015 ◽  
Vol 72 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sri A. Febriana ◽  
Erik Zimerson ◽  
Cecilia Svedman ◽  
Winarto Haryadi ◽  
Pieter-Jan Coenraads ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Layer Chromatography

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

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