scholarly journals Evaluation of antibacterial activity of three different glass ionomer cements on streptococcus mutans: an in-vitro antimicrobial study.

2019 ◽  
Author(s):  
Chhaya Harin Khere ◽  
Hemalatha Hiremath ◽  
Sandesh N ◽  
Pooja Misar ◽  
Nishat Gorie
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Antimicrobial Property ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
High Caries Risk

Aim. Purpose of this in-vitro study was to asses and compare the antimicrobial activity of three different glass ionomer cements (GIC) against streptococcus mutans (S. Mutans) bacteria using agar plate diffusion test. Methods. Thirty blood agar plates were prepared and three wells of 4mm diameter were made on each agar plate. Three different GIC (Micron bioactive, GC Fuji IX GP Extra, Bioglass r) were mixed and filled into the wells. These plates were inoculated with S. Mutans and incubated at 37°C for 24 hours. Bacterial growth inhibition zone around each well were recorded in millimeters using Hiantibiotic Zonescale-C. Result. All the restorative material used in the study exhibited antimicrobial property against S. Mutans. GC Fuji IX GP Extra showed superior antimicrobial efficacy with 17.3±2.6 mm mean diameter of bacterial inhibition zone, followed by Micron bioactive 14.4±1.07 mm and Bioglass r 10.8 ± .91 mm inhibition zone respectively. Conclusion. Within the limitation of this study, it can be concluded that all the GIC evaluated demonstrated antibacterial activity against S. Mutans. The superior antimicrobial activity was demonstrated by GC Fuji IX GP Extra. Hence, it could be advantageous in patients with high caries risk.

Antibacterial Activity In Vitro of the Developed Glass Ionomer Cements

2011 ◽  
Vol 493-494 ◽  
pp. 375-380
Author(s):  
Joanna Karaś ◽  
Andrzej Roman Olszyna ◽  
Ewa Zaczyńska ◽  
Anna Czarny ◽  
Krzysztof Szamałek
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Initial Period ◽  
Control Culture ◽  
Fluoride Release ◽  
Glass Ionomer Cements ◽  
Glass Ionomer ◽  
Bacterial Strains ◽  
Daily Amount

The purpose of the study was to identify the influence of the amount of fluoride release from glass ionomer cements on survival of bacterial strains responsible for the development of secondary caries. All developed cements had an antibacterial impact on the strains used in the study. The study demonstrated that the cements exhibit greater antibacterial activity againstStreptococcus mutansthanStreptococcus sanguinis. The numbers of bacteria count in cultures on the developed cements were approximately 10 times lower compared to the control culture after 24 h, 48 h, 72 h and 7 days. The developed cements had initially (1-2 days) greater antibacterial impact against the strains used in the study,which corresponds to the greatest daily amount of fluoride released from the cements. Following the initial period, the antibacterial activity of all the cements remained roughly at the same level.

scholarly journals In Vitro Antimicrobial, Antiviral and Cytotoxicity Activities of Aspergillus oryzae Isolated From El-Baida Marsh in Algeria

2020 ◽  
Vol 10 (4) ◽  
pp. 191-195
Author(s):  
Nacef Houda Sara ◽  
Belhattab Rachid ◽  
Galvez Julio ◽  
Rodriguez-Sojo María Jesus ◽  
Vezza Teresa
Keyword(s):  
Antimicrobial Activity ◽  
Antiviral Activity ◽  
Aspergillus Oryzae ◽  
Antimicrobial Property ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Host Cells ◽  
Minimal Inhibitory Concentrations ◽  
Cytotoxicity Activity

This work covers the study of antimicrobial and antiviral activities of the Aspergillus oryzae strain isolated from saline soil (El-Baida marsh in Algeria). The crude extract obtained with ethyl acetate displayed antimicrobial activity against Gram-positive and Gram-negative bacteria and the yeast Candida albicans with a mean of 16.69 mm of inhibition zone and a minimal inhibitory concentrations MICs between 7.28 and 21.85 μgmL-1. We also assessed the antiviral activity against Herpes simplex-2 Virus (HSV-2), in which no inhibitory effect was exhibited. In addition, cytotoxicity activity was tested in Caco-2 and RAW 264, a human epithelial and a murine macrophage cell line, respectively, revealing a no-toxic effect of the extract. The studied isolate extract possesses an antimicrobial property and its non-toxicity to the host cells becomes very important, and can be exploited for the production of new pharmacological and biotechnological agents.        Keywords: Aspergillus oryzae, antimicrobial activity, antiviral activity, cytotoxicity, fungal extraction.

scholarly journals The effect of the addition of propolis to resin modified glass ionomer cement bracket adhesive materials on the growth inhibition zone of Streptococcus mutans

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 2105
Author(s):  
Stefani Kristanti Saputra ◽  
Darmawan Sutantyo ◽  
Cendrawasih Andusyana Farmasyanti ◽  
Ananto Ali Alhasyimi
Keyword(s):  
Growth Inhibition ◽  
Streptococcus Mutans ◽  
Glass Ionomer Cement ◽  
Agar Plate ◽  
Inhibition Zone ◽  
Glass Ionomer ◽  
Growth Inhibition Zone ◽  
Resin Modified Glass Ionomer ◽  
Ionomer Cement ◽  
Adhesive Materials

Background: Orthodontic treatments progress alongside the development of adhesive materials. The aim of the present study was to determine the antibacterial properties of propolis, a natural product, in a mixture of resin modified glass ionomer cement by observing the growth inhibition zone of Streptococcus mutans. Methods: This was an in vitro study conducted on 45 samples of adhesive material, which were divided into three groups of propolis concentrations (0%, 15%, and 25%) and duration (0, 15, and 30 days). The antibacterial effect of each sample was evaluated against S. mutans using an agar plate diffusion test. Measurement of the diameter of the growth inhibition zone of S. mutans were carried out. The data obtained were analyzed statistically by Kruskal Wallis test. Results: There was a relationship between concentration and duration of propolis to the growth inhibition zone of S. mutans (p<0.05). The addition of 25% propolis concentration inhibited the growth of S. mutans more than the addition of 15% and 0% propolis concentration. The addition of 0%, 15%, and 25% propolis concentration to resin modified glass ionomer cement for 15 days was more effective in inhibiting the growth of S. mutans. Conclusion: The addition of propolis to adhesive materials provides an inhibitory effect on the growth of S. mutans, which may be effective in the world of preventive dentistry.

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

2018 ◽  
Vol 9 (3) ◽  
pp. 336
Author(s):  
Ni Luh Budi Artaningsih ◽  
Nur Habibah ◽  
Mastra Nyoman
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Gliricidia Sepium ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone

<p><em>Streptococcus mutans</em> is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>) for <em>Streptococcus mutans</em> in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of <em>Streptococcus mutans</em> in various concentrations of ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (<em>Gliricidia sepium</em>) have strong antibacterial activity, especially to <em>Streptococcus mutans</em> bacteria.</p>

scholarly journals Antibacterial efficacy of spice extracts against Streptococcus mutans and Lactobacillus acidophilus – An in-vitro study

2021 ◽  
Vol 4 ◽  
pp. 73-77
Author(s):  
Pooja Latti ◽  
R. Subramaniam ◽  
G. M. Prashant
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Lactobacillus Acidophilus ◽  
Black Pepper ◽  
Maximum Activity ◽  
Diffusion Method ◽  
Local Market ◽  
Zone Of Inhibition

Objectives: To evaluate the antibacterial activity of black pepper, Indian bay leaf, cinnamon, and cumin against Streptococcus mutans and Lactobacillus acidophilus in-vitro and to determine their minimum inhibitory concentration (MIC). Materials and Methods: The spices (cinnamon, cumin, Indian bay leaf, and black pepper) were obtained from local market, were dried and powdered. Solvent extracts were prepared with methanol by maceration, followed by filtration and evaporation. The antimicrobial activity was assessed using cup plate diffusion method, followed by determination of MIC of the extracts. Statistical analysis was performed using one-way analysis of variance and Tukey’s post hoc test was used for pairwise comparison. P < 0.05 was considered statistically significant. Results: All the four extracts showed significant antimicrobial activity. Cinnamon demonstrated maximum activity against S. mutans (zone of inhibition of 18.1 mm ± 0.30) and L. acidophilus (zone of inhibition of 17.9 mm ± 0.44) with the least MIC against the organisms (<0.05 mg/ml). Conclusion: All the spice extracts tested demonstrated significant antibacterial activity against S. mutans and L. acidophilus. On comparison of the antibacterial activities of all the four extracts, cinnamon extract emerged as the potent agent.

scholarly journals Evaluation of the Shear Bond Strength and Antibacterial Activity of Orthodontic Adhesive Containing Silver Nanoparticle, an In-Vitro Study

Nanomaterials ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1466
Author(s):  
Ladan Eslamian ◽  
Ali Borzabadi-Farahani ◽  
Shahedeh Karimi ◽  
Sepideh Saadat ◽  
Mohammad Reza Badiee
Keyword(s):  
Stainless Steel ◽  
Antibacterial Activity ◽  
Bond Strength ◽  
Streptococcus Mutans ◽  
Shear Bond Strength ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Agar Diffusion Test ◽  
Significant Difference

This study evaluated the effect of incorporating silver nanoparticles (AgNPs) into conventional orthodontic adhesive on its antibacterial activity and the shear bond strength (SBS) to stainless steel orthodontic brackets. Thirty-four extracted premolars were randomly allocated into two groups (n = 17). Orthodontic adhesive (Transbond XT, 3M Unitek) was blended with AgNPs (50 nm, 0.3% w/w) to form a nano-adhesive. In order to bond stainless steel twin brackets (0.022-inch, American Orthodontics), Transbond XT (n = 17) and nano-adhesive (n = 17) were used in each group, respectively, after acid etching (37% phosphoric acid, 30 s) and rinsing with water (15 s). SBS and the adhesive remnant index (ARI) scores were recorded. Antibacterial activity against Streptococcus mutans in both groups after 24 h and 30 days was assessed (Disc agar diffusion test) and the inhibition zone diameter around each specimen was measured and recorded. Adding AgNPs significantly (p = 0.009) reduced the mean (SD) SBS in the nano-adhesive group [10.51(7.15) MPa] compared to Transbond XT [17.72(10.55) MPa]. The ARI scores on the Transbond XT and nano-adhesive showed no statistically significant difference (p = 0.322). Nano-adhesive with AgNPs showed significant antibacterial activity against Streptococcus mutans at 24 h and 30 days (p < 0.001). In both groups, no significant decline in the zones of inhibition was detected after 30 days (p = 0.907). The findings suggest that SBS decreased after incorporation of AgNPs [0.3% (w/w)], but was still above the recommended SBS of 5.9–7.8 MPa. The nano-adhesive showed significant antibacterial activity which did not change much after 30 days.

Effect of Acid and Fluoride Release from Four Glass Ionomer Cements on Streptococcus mutans: An In Vitro Study

2015 ◽  
Vol 1 ◽  
pp. 19-22
Author(s):  
Simpy Amit Mahuli ◽  
Amit Vasant Mahuli ◽  
R. Subramaniam ◽  
Mahesh Hiregoudar ◽  
G. M. Prashant ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
In Vitro Study ◽  
Vitro Study ◽  
Fluoride Release ◽  
Glass Ionomer Cements ◽  
Glass Ionomer

scholarly journals SYNTHESIS AND UTILITY OF NEW POLYCYCLIC COMPOUNDS AS POTENTIAL ANTIMICROBIALS BASED ON CHROMENE MOIETY

2019 ◽  
pp. 49-56
Author(s):  
MAHMOUD N. ABDELAZIZ ◽  
EMAN S. ZARIE ◽  
ALAADIN E. SARHAN
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Condensation Reaction ◽  
Ethyl Cyanoacetate ◽  
Inhibition Zone ◽  
Phenyl Isothiocyanate ◽  
Polycyclic Compounds ◽  
E Coli ◽  
Multiple Species

Objective: The present research aims to synthesize some new polycyclic compounds including chromene moiety and study their antimicrobial activity. Methods: Several new polycyclic systems including chromene scaffold incorporated with pyridine, pyrimidine, imidazopyrimidine, and imidazodiazocine were achieved via condensation reaction of chromene derivative under the proper condition with various reagents namely; cyanothioacetamide, phenyl isothiocyanate, malononitrile, carbon disulfide, benzaldehyde, triethylorthoformate, and 1,4-dichlorobutane. Moreover, a chlorodiazenyl chromene derivative was reacted with some substances possessing active–CH2-bridge such as ethyl cyanoacetate and malononitrile to end up with hydrazono compounds. Such compounds were eventually cyclized with hydrazine hydrate to form pyrazole and oxopyrazole derivatives. Moreover, compound 1 was treated with benzoyl acetone, and then followed by cyclization with malononitrile to provide the corresponding 2-amino14-(4-methoxyphenyl)-4-methy-5-phenyl-14H-benzo[5,6] chromeno[2,3H][1,6]naphthyridine-3-carbonitrile (20). Results: The results of the antimicrobial screening in vitro revealed that the inhibition zone (mm) of the synthesized compounds 1-3, 5 and 8 implied their optimum antibacterial activity, while the compounds 4, 6 and 9-13, 15 showed a moderate to weak antibacterial activity against multiple species of B. subtilis, S. aureus, E. coli and P. aeruginosa. In contrast, the compounds 1, 6, 11, 15 showed high antifungal activities against different species of A. flavinand C. albicans, while the other compounds exhibit a moderate to poor antifungal activity. Conclusion: It is remarkable that a series of chromene derivatives synthesized by a simple and available method leads to a molecule of promising antimicrobial activity. Further research is recommended to approve the importance of polycyclic systems for various applications.

scholarly journals Evaluation of the Antimicrobial Effect of Mineral Trioxide Aggregate Mixed with Fluorohydroxyapatite against E. faecalis In Vitro

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Behnam Bolhari ◽  
Aidin Sooratgar ◽  
Maryam Pourhajibagher ◽  
Nazanin Chitsaz ◽  
Iman Hamraz
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Growth Inhibition ◽  
Mineral Trioxide Aggregate ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Statistical Analyses ◽  
Biofilm Inhibition ◽  
Growth Inhibition Zone

Enterococcus faecalis is the dominant microorganism in chronic apical periodontitis. It is more resistant to local antiseptic agents than other endodontic microorganisms. Currently, mineral trioxide aggregate (MTA) is considered as an ideal material in many endodontic procedures. Some studies have shown that MTA has good antibacterial activity against E. faecalis. However, some studies have investigated the effect of incorporating some materials into MTA on its antibacterial activity against E. faecalis. No study has evaluated the effect of incorporating fluorohydroxyapatite nanoparticles (nano-FHA) on the antimicrobial activity of MTA. Therefore, the present study evaluated the antimicrobial effect of MTA mixed with nano-FHA on E. faecalis in vitro. The study was carried out on 18 samples in three groups: pure MTA, MTA mixed with 10 wt% of nano-FHA, and MTA mixed with 15 wt% of nan-FHA. The effect of nano-FHA on the antibacterial activity of MTA on E. faecalis was evaluated by evaluating the growth inhibition zone around each sample. The antimicrobial effect of samples on inhibiting E. faecalis biofilm formation and inhibiting microbial growth of E. faecalis in the planktonic phase was evaluated by disk agar diffusion (DAD), biofilm inhibition assay (BIA), and direct contact assay (DCA) tests, respectively. All the above tests were analyzed after 24 and 72 hours. Factorial designs were used for statistical analyses. Tukey tests were used for two-by-two comparisons. All the statistical analyses were carried out with SPSS 26. DAD results showed no formation of the growth inhibition zone in all the samples after 24 and 72 hours. The microbial colony counts in the BIA and DCA tests in the groups modified with FHA nanoparticles were significantly lower than the pure MTA group ( P < 0.05 ). The microbial colony counts increased in all the groups over time ( P < 0.05 ). Incorporating nano-FHA into MTA improved the antimicrobial activity of MTA against E. faecalis compared to pure MTA. The highest antimicrobial activity was achieved after incorporating 15 wt% of nano-FHA into MTA at the 72-hour interval.

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