Evaluation of the Antimicrobial Effect of Mineral Trioxide Aggregate Mixed with Fluorohydroxyapatite against E. faecalis In Vitro

Enterococcus faecalis is the dominant microorganism in chronic apical periodontitis. It is more resistant to local antiseptic agents than other endodontic microorganisms. Currently, mineral trioxide aggregate (MTA) is considered as an ideal material in many endodontic procedures. Some studies have shown that MTA has good antibacterial activity against E. faecalis. However, some studies have investigated the effect of incorporating some materials into MTA on its antibacterial activity against E. faecalis. No study has evaluated the effect of incorporating fluorohydroxyapatite nanoparticles (nano-FHA) on the antimicrobial activity of MTA. Therefore, the present study evaluated the antimicrobial effect of MTA mixed with nano-FHA on E. faecalis in vitro. The study was carried out on 18 samples in three groups: pure MTA, MTA mixed with 10 wt% of nano-FHA, and MTA mixed with 15 wt% of nan-FHA. The effect of nano-FHA on the antibacterial activity of MTA on E. faecalis was evaluated by evaluating the growth inhibition zone around each sample. The antimicrobial effect of samples on inhibiting E. faecalis biofilm formation and inhibiting microbial growth of E. faecalis in the planktonic phase was evaluated by disk agar diffusion (DAD), biofilm inhibition assay (BIA), and direct contact assay (DCA) tests, respectively. All the above tests were analyzed after 24 and 72 hours. Factorial designs were used for statistical analyses. Tukey tests were used for two-by-two comparisons. All the statistical analyses were carried out with SPSS 26. DAD results showed no formation of the growth inhibition zone in all the samples after 24 and 72 hours. The microbial colony counts in the BIA and DCA tests in the groups modified with FHA nanoparticles were significantly lower than the pure MTA group ( P < 0.05 ). The microbial colony counts increased in all the groups over time ( P < 0.05 ). Incorporating nano-FHA into MTA improved the antimicrobial activity of MTA against E. faecalis compared to pure MTA. The highest antimicrobial activity was achieved after incorporating 15 wt% of nano-FHA into MTA at the 72-hour interval.

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Antimicrobial Effect of Newly Formulated Toothpastes and a Mouthrinse on Specific Microorganisms: An In Vitro Study

European Journal of Dentistry ◽

10.1055/s-0039-1695655 ◽

2019 ◽

Vol 13 (02) ◽

pp. 172-177 ◽

Cited By ~ 2

Author(s):

Yeliz Guven ◽

Nilufer Ustun ◽

Elif Bahar Tuna ◽

Oya Aktoren

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Bacterial Species ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Antimicrobial Effect ◽

Inhibition Zone ◽

Vitro Study

Abstract Objective The aim of this in vitro study was to assess the antimicrobial properties of newly formulated toothpastes (four toothpastes for adults and two toothpastes for kids/babies) and a mouthrinse. Materials and Methods Newly formulated six different toothpastes and one mouthrinse of a single brand and commercially available five toothpastes and three mouthrinse were investigated for their antimicrobial activity against two oral pathogens, Streptococcus mutans and Candida albicans, by agar well diffusion assay. After incubation, the inhibition zone diameters were measured in millimeters and statistical analyses were performed. Results All experimental adult toothpastes exhibited good antimicrobial activity against S. mutans and C. albicans except the experimental toothpaste D. Experimental toothpaste B exhibited the highest antibacterial activity against C. albicans and S. mutans. Experimental toothpaste for kids showed the best antimicrobial activity against S. mutans when kids’ toothpastes were compared. None of the tested toothpastes for kids/babies showed antibacterial effects for C. albicans. Among the mouthrinse tested, Sensodyne mouthrinse showed the best results. Experimental mouthrinse showed significantly lower antibacterial activity against S. mutans then Sensodyne, Eludril, and chlorhexidine mouthrinse. Conclusion Although experimental toothpaste and mouthrinse formulations revealed good results in terms of antimicrobial activity to some specific microorganisms, further studies involving more bacterial species or analyzing the quality and efficacy of these products by other in vitro or in vivo tests are needed.

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Antimicrobial Activity of two Mentha Species Essential Oil and its Dependence on Different Origin and Chemical Diversity

Natural Product Communications ◽

10.1177/1934578x1801300832 ◽

2018 ◽

Vol 13 (8) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Mária Pl'uchtová ◽

Teresa Gervasi ◽

Qada Benameur ◽

Vito Pellizzeri ◽

Daniela Grul'ová ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oil ◽

Inhibition Zone ◽

Chemical Diversity ◽

Diffusion Method ◽

Salmonella Spp ◽

Disk Diffusion Method ◽

Gram Positive Bacteria ◽

Growth Inhibition Zone

Genus Mentha presents group of plants which are the most studied in family Lamiaceae. Aboveground parts are used for different purposes in pharmacy, food industry or confectionery. Most important is natural product extracted from leaves - essential oil (EO). The aim of presented experiment was to demonstrate different chemotype and compare antibacterial activity of two Mentha species EO. Plant samples were obtained from various environments – from Slovakia and from Italy. Dominant compounds were determined by GC/MS. The results showed high amount of menthol and menthone in tested Slovak peppermint EO. On the other hand, carvone and 1,8-cineole were determinate as dominant compounds in Italian spearmint EO. The antimicrobial activity of the EO was investigated by disc diffusion and broth micro dilution methods. EO was evaluated for their antibacterial activity against 7 microorganisms: Enterobacter cloacae, Salmonella spp., Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes. The determination results of antibacterial activity by agar disk diffusion method ranged from 7 to 14 mm of the growth inhibition zone. MIC of tested mint EO varied from 0.625 to 2.5 μg/mL. In addition, both EO showed relatively the same antibacterial activity against the selected Gram-negative bacteria. However, there is a variation in the antibacterial activity against Gram-positive bacteria.

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KEEFEKTIFAN EKSTRAK MENGKUDU PADA BERBAGAI KONSENTRASI TERHADAP PENGHAMBATAN PERTUMBUHAN BAKTERI RALSTONIA SP. SECARA IN VITRO

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA ◽

10.23960/j.hptt.2483-88 ◽

2004 ◽

Vol 4 (2) ◽

pp. 83-88

Author(s):

Efri Efri ◽

Titik Nur Aeny

Keyword(s):

Growth Inhibition ◽

Growth Inhibitor ◽

Inhibition Zone ◽

Morinda Citrifolia ◽

In Vitro Growth ◽

Fruit Extract ◽

Bacterial Wilt Disease ◽

Growth Inhibition Zone ◽

Medicinal Properties

Effectivity of Morinda citrifolia extract on in-vitro growth inhibition of Ralstonia sp. Morinda citrifolia has been known as one of plants having numerous medicinal properties. Extract of the fruits shows antibacterial activity against several bacteria including Pseudomonas aeruginosa. The purpose of this study was to observe inhibitor effect of M. citrifolia fruit extract, and to investigate the effect of different concentrations of M. citrifolia fruit extract on in-vitro growth of Ralstonia (Pseudomonas) sp., the causal agent of banana bacterial wilt disease in Lampung. Inhibition effect of M. citrifolia was determined by the presence of inhibition zone, and concentration effect was determined by the diameter of inhibition zone . The study consisted of two experiments. The treatments of the first experiment were three different kinds of fruit extract, namely the whole fruit, the seeds, and the fleshy parts, and the second one consisted of six different levels of extract concentrations. The results showed that the whole fruit extract was the most effective as an in-vitro growth inhibitor, and the higher the concentration level the higher the growth inhibition zone formed.

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Infestation of tall fescue (Festuca arundinacea Schreb.) with Neotyphodium coenophialum and its influence on growth of chosen microorganisms in vitro

Acta Agrobotanica ◽

10.5586/aa.2005.063 ◽

2012 ◽

Vol 58 (2) ◽

pp. 369-380 ◽

Cited By ~ 4

Author(s):

Dariusz Pańka

Keyword(s):

Growth Inhibition ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Inhibition Zone ◽

Bipolaris Sorokiniana ◽

Gaeumannomyces Graminis ◽

Neotyphodium Coenophialum ◽

Mycelium Growth ◽

Growth Inhibition Zone

Occurrence of Neotyphodium coenophialum in tall fescue cultivars cultivated in Poland and determination an endophyte inhibition effect on mycelium growth of chosen microorganisms in vitro were investigated. Seventeen seed lots of 11 cultivars of tall fescue were examined. The endophyte mycelium was dyed with bengal rose and microscopically examined to detect N. coenophialum. Occurrence of endophyte was checked with PCR method. Influence of endophyte on growth of 15 microorganisms was established in the laboratory conditions on Petri dishes with PDA medium at 10, 20 and 30°C. Neotyphodium coenophialum occurred only in two seed lots, 'Barrocco' - 42% and Terros - 2%. Living mycelium of endophyte was isolated only from 'Barrocco'. The highest mycelium growth inhibition of Bipolaris sorokiniana, Fusarium avenaceum, F. equiseti, Microdochium nivale and Gaeumannomyces graminis by endophyte at 30°C was recorded. The highest width of growth inhibition zone (4mm) was detected for the last pathogen. Mycelium growth of B. sorokiniana and M. nivale was not inhibited at 10°C, and for F. avenaceum at 10 and 20°C.

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PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI REBUSAN DAUN SALAM (Syzygium polyanthum) SECARA IN VITRO

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v5i2.138 ◽

2018 ◽

Vol 5 (2) ◽

Author(s):

Nyoman Mastra

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Average Diameter ◽

Inhibition Zone ◽

Negative Control ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone ◽

Significant Difference

AbstractBackground Staphylococcus aureus is one of causes infection and this bacteria have been resistence for many antibiotic. Bay leaf have antibacterials substance, which stew leaves can be treat infection caused Staphylococcus aureus. The purpose of this study was to determine differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf.Method The method of this study is true experiment with posstest only control design, and used Kirby Bauer disk diffusion method with various concentrations of water stewed of bay leaf (20%, 40%, 60%, 80%,100%), positive control (chloramfenicol 30 μg) and negative control (sterile distilled water). Result The result showed that the average diameter of inhibition zone in concentration 20%, 40%, 60%, 80%, and 100% is 7 mm, 8,4 mm, 9,6 mm, 10,5 mm and 11,5 mm. Based on statistical analysis using oneway ANOVA available the value of p (0,000) α (0,05), so the inhibition zone is significant difference of growth inhibition zone of Staphylococcus aureus at various concentrations of stewed water bay leaf. Conclusion Water stew of bay leaf can inhibit the growth of Staphylococcus aureus, and there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of water stew of bay leaf. Keywords: stew of bay leaf; Staphylococcus aureus; inhibition zone

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Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

Jurnal Kesehatan ◽

10.26630/jk.v9i3.967 ◽

2018 ◽

Vol 9 (3) ◽

pp. 336

Author(s):

Ni Luh Budi Artaningsih ◽

Nur Habibah ◽

Mastra Nyoman

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Ethanol Extract ◽

Inhibition Zone ◽

Gliricidia Sepium ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone

Streptococcus mutans is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (Gliricidia sepium) for Streptococcus mutans in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of Streptococcus mutans in various concentrations of ethanol extract of Gamal leaf (Gliricidia sepium). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (Gliricidia sepium) have strong antibacterial activity, especially to Streptococcus mutans bacteria.

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PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

Meditory : The Journal of Medical Laboratory ◽

10.33992/m.v6i1.227 ◽

2018 ◽

Vol 6 (1) ◽

Author(s):

Nyoman Mastra

Keyword(s):

Staphylococcus Aureus ◽

Growth Inhibition ◽

Pathogenic Bacteria ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone ◽

The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

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Antimicrobial activity of medicinal plants used for urinary tract infections in pastoralist community in Ethiopia

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-021-03249-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Eshetu Gadisa ◽

Elazar Tadesse

Keyword(s):

Antimicrobial Activity ◽

Urinary Tract ◽

Medicinal Plants ◽

Urinary Tract Infections ◽

Antimicrobial Effect ◽

Inhibition Zone ◽

Resistant Bacteria ◽

Chemical Structures ◽

Tract Infections

Abstract Background Medicinal plants have wide medicament application used to prevent and management of many ailments. These plants are used for primary health care in pastoralist communities who are deprived of modern medical care. They possess extensive therapeutics bioactive coupled with varied chemical structures. However, scientific validation of efficacy and safety of plants used to treat the urinary tract infections haven’t been fully exploited. The aim of this study was to evaluate antimicrobial activity and screening phytochemicals of medicinal plants used to treat urinary tract infections. Methods In-vitro experimental study was carried out to evaluate the antimicrobial effect and screening phytochemical of Rumex abyssinicus, Cucumis pustulatus, Discopodium penninervium, Lippia adoensis, Euphorbia depauperata, and Cirsium englerianum. Against drug resistance microbes. 80% methanol was used for extraction of the plant parts. The susceptibility tests were investigated using disc diffusion and broth micro-dilution methods. Results The majority of tested extracts showed antimicrobial activity on two or more drug-resistant bacteria with MIC value (1.0–128.0 μg/ml) and 9–27 mm inhibition zone in diameter. Extracts obtained from C.englerianum and E. depauperate showed more potent antibacterial activity on MRSA and Enterococcus faecalis with IZ 25 and 27 mm respectively. E. coli and K. pneumoniae were inhibited by those extracts with IZ ranging 9–25 mm and 11–27 mm respectively. E.faecalis and K. pneumoniae were more susceptible bacteria to the respective extracts. R. abyssinicus showed promising antifungal effect with had 21 mm IZ and MIC range 16-32 μg/ml on C.albicans. Alkaloids, flavonoids, phenolic and terpenoid were common phytochemical characterized in majority of screened plants. Conclusion Tested extracts exhibited significant antibacterial and antifungal activity. Hence, further structural elucidation of bioactive that inhibited the growth of microbes aforementioned plants may be used as precursors for the synthesis of new antibiotics in the future.

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Antibacterial Effect of Hydroalcoholic Extract ofPunica granatumLinn. Petal on Common Oral Microorganisms

International Journal of Biomaterials ◽

10.1155/2016/8098943 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 3

Author(s):

Farnaz Hajifattahi ◽

Elham Moravej-Salehi ◽

Maryam Taheri ◽

Arash Mahboubi ◽

Mohammad Kamalinejad

Keyword(s):

Growth Inhibition ◽

Antibacterial Effect ◽

Brain Heart Infusion ◽

Inhibition Zone ◽

Maximum Effect ◽

Streptococcus Sobrinus ◽

Bacterial Strains ◽

Hydroalcoholic Extract ◽

Growth Inhibition Zone

Objectives. This study aimed to assess the effect of hydroalcoholic extract ofPunica granatumLinn. (P. granatum) petal onStreptococcus sanguinis,Streptococcus mutans,Streptococcus salivarius,Streptococcus sobrinus,andEnterococcus faecalis.Materials and Methods. In this in vitro study,P. granatumextract was prepared using powdered petals and water-ethanol solvent. Antibacterial effect of the extract, chlorhexidine (CHX), and ampicillin was evaluated on brain heart infusion agar (BHIA) using the cup-plate method. By assessing the diameter of the growth inhibition zone, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract were determined for the above-mentioned bacteria.Results. Hydroalcoholic extract ofP. granatumpetal had inhibitory effects on the proliferation of all five bacterial strains with maximum effect onS. mutanswith MIC and MBC of 3.9 mg/mL. The largest growth inhibition zone diameter belonged toS. sanguinisand the smallest toE. faecalis. Ampicillin and CHX had the greatest inhibitory effect onS. sanguinis.Conclusions. Hydroalcoholic extract ofP. granatumhad a significant antibacterial effect on common oral bacterial pathogens with maximum effect onS. mutans, which is the main microorganism responsible for dental plaque and caries.

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THE ANTIBACTERIAL ACTIVITY OF SELECTED PHARMACOLOGICAL AGENTS

Canadian Journal of Microbiology ◽

10.1139/m65-085 ◽

1965 ◽

Vol 11 (4) ◽

pp. 637-640

Author(s):

Alfred A. Richtarik ◽

Klaus Lindemulder ◽

B. De Boer

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antimicrobial Effect ◽

Pharmacological Agents ◽

Phenoxymethyl Penicillin ◽

In Vitro Antimicrobial Activity ◽

Phage Types ◽

Bacteriostatic Action

The in vitro antimicrobial activity of selected pharmacological agents was studied against five strains of staphylococci (phage types 7 US, 54, 71, 80, 81). At the concentrations used (maximal 0.125 μg/ml) chlorothiazide, acetazolamide, FeCl3, SnCl2, stilbamine urea, quinacrine, antimony Na thioglycollate, antimony thioglycollamide, and sulfisoxazole were found to have no antimicrobial effect against these strains of staphylococci, whereas HgCl2, AgNO3, meralluride sodium, stilbamidine, pentamidine, and propamidine did show activity. When effective agents were combined with potassium phenoxymethyl penicillin, the bacteriostatic action of the antibiotic was inhibited in some cases and markedly potentiated in others. Consideration should be given to the possible incorporation of effective agents into the structure of synthetic antibiotics as a means of combating resistant staphylococci.

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