scholarly journals p16INK4/Ki-67 Dual-Staining Expression as a Prognostic Indicator in Laryngeal Cancer

2014 ◽  
Vol 1 (3) ◽  
Author(s):  
Elisabetta Carico
Keyword(s):  
Laryngeal Cancer ◽  
Prognostic Indicator ◽  
Ki 67 ◽  
Dual Staining

scholarly journals The expression and significance of AKR1B10 in laryngeal squamous cell carcinoma

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jixuan Liu ◽  
Hongyan Ban ◽  
Yafang Liu ◽  
Jinsong Ni
Keyword(s):  
Laryngeal Cancer ◽  
Laryngeal Carcinoma ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Lipid Synthesis ◽  
Prognostic Indicator ◽  
Carcinoma Cells ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Ki 67 ◽  
Degree Of Differentiation

AbstractAldosterone reductase family 1 member B10 (AKR1B10) is a nicotinamide adenine dinucleotide phosphate (reduced coenzyme II)-dependent oxidoreductase, and its biological functions include carbonyl detoxification, hormone metabolism, osmotic adjustment, and lipid synthesis. Studies suggested that AKR1B10 is a new biomarker for cancer based on its overexpression in epithelial tumors, such as breast cancer, cervical cancer, and lung cancer. At present, studies on the expression of AKR1B10 in laryngeal cancer have not been reported. However, we found that AKR1B10 is upregulated in laryngeal carcinoma, and its expression was negatively correlated with the degree of differentiation. In addition, AKR1B10 expression was positively correlated with tumor size; lymph node metastasis; alcohol use; and Ki-67, mutant p53, and matrix metalloproteinase 2 expression. AKR1B10 was overexpressed in Hep-2 laryngeal carcinoma cells. Oleanolic acid inhibited AKR1B10 activity and expression in Hep-2 cells and suppressed Hep-2 cell proliferation, migration, and invasion. Therefore, AKR1B10 may be related to the development of laryngeal carcinoma, suggesting its use as a prognostic indicator for laryngeal cancer.

scholarly journals The expression and significance of AKR1B10 in laryngeal squamous cell carcinoma

2021 ◽  
Author(s):  
Jixuan Liu ◽  
Hongyan Ban ◽  
Yafang Liu ◽  
Jinsong Ni
Keyword(s):  
Laryngeal Cancer ◽  
Laryngeal Carcinoma ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Lipid Synthesis ◽  
Prognostic Indicator ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Ki 67 ◽  
Degree Of Differentiation

Abstract Aldosterone reductase family 1 member B10 (AKR1B10) is a nicotinamide adenine dinucleotide phosphate (NADPH) (reduced coenzyme II)-dependent oxidoreductase, and its biological functions include carbonyl detoxification, hormone metabolism, osmotic adjustment, and lipid synthesis. Studies suggested that AKR1B10 is a new biomarker for cancer based on its overexpression in epithelial tumors, such as breast cancer, cervical cancer, and lung cancer. At present, studies on the expression of AKR1B10 in laryngeal cancer have not been reported. However, we found that AKR1B10 is upregulated in laryngeal carcinoma, and its expression was negatively correlated with the degree of differentiation. In addition, AKR1B10 expression was positively correlated with tumor size; lymph node metastasis; long-term drinking; and Ki-67, mutant p53, and matrix metalloproteinase 2 expression. AKR1B10 was overexpressed in Hep-2 laryngeal carcinoma cells. Oleanolic acid inhibited AKR1B10 activity and expression in Hep-2 cells and suppressed Hep-2 cell proliferation, migration, and invasion. Therefore, AKR1B10 may be related to the development of laryngeal carcinoma, suggesting its use as a prognostic indicator for laryngeal cancer.

Interlaboratory concordance of p16/Ki‐67 dual‐staining interpretation in HPV‐positive women in a screening population

2020 ◽  
Vol 128 (5) ◽  
pp. 323-332 ◽  
Author(s):  
Maria Benevolo ◽  
Pamela Mancuso ◽  
Elena Allia ◽  
Daniela Gustinucci ◽  
Simonetta Bulletti ◽  
...  
Keyword(s):  
Ki 67 ◽  
Screening Population ◽  
Dual Staining

Role of Ancillary Techniques in Gynecologic Cytopathology Specimens

2019 ◽  
Vol 64 (1-2) ◽  
pp. 63-70
Author(s):  
Andrea D. Olivas ◽  
Julieta E. Barroeta ◽  
Ricardo R. Lastra
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Screening ◽  
High Risk ◽  
Cervical Cancer Screening ◽  
Hpv Testing ◽  
Invasive Procedures ◽  
Ki 67 ◽  
Dual Staining

The association between high-risk genotypes of human papillomavirus (hr-HPV) and cervical cancer is well established. As hr-HPV testing is rapidly becoming a part of routine cervical cancer screening, either in conjunction with cytology or as primary testing, the management of hr-HPV-positive women has to be tailored in a way that increases the detection of cervical abnormalities while decreasing unnecessary colposcopic biopsies or other invasive procedures. In this review, we discuss the overall utility and strategies of hr-HPV testing, as well as the advantages and limitations of potential triage strategies for hr-HPV-positive women, including HPV genotyping, p16/Ki-67 dual staining, and methylation assays.

The prognostic VALUE of Ki-67 in gastric cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e15172-e15172
Author(s):  
Antonio Caldeira Fradique ◽  
Ligia Bruno Da Costa ◽  
Alexandra Pupo ◽  
Luisa Quaresma ◽  
Gualdino Silva ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Carcinoma ◽  
Lymph Nodes ◽  
Cancer Mortality ◽  
Prognostic Value ◽  
Prognostic Indicator ◽  
Nutrition Status ◽  
Depth Of Invasion ◽  
Tumor Type ◽  
Ki 67

e15172 Background: Gastric carcinoma is the second cause of cancer mortality worldwide. In Portugal, a country with a high incidence of the disease, it is also the second leading cause of cancer mortality. The gravity of this situation and the advanced stage of disease at presentation warrants a search of a new prognostic indicator. We studied in patients (pts) with gastric cancer, both diffuse and intestinal types, several possible prognostic indicators, such as: weight, nutrition status, Ki-67, p53, Cerb2, and cadherin E. The results showed that Ki-67, an indicator of cell proliferation, may play an important role as a prognostic indicator because it is easily assessed by immunohistochemistry, is observed in both intestinal and in diffuse tumor types, and is related with tumor size, depth of invasion and the pattern of lymphatic spread. Methods: A total of 50 pts with gastric carcinoma (25 diffuse and 25 intestinal types) undergoing radical gastrectomy with extended lymphadenectomy (D2 and a few D3 types ) were prospectively studied by immunohistochemistry for the determination of Ki-67 in biopsied tissue and gastrectomy specimens. A relationship between the Ki-67 and various other prognostic factors, like histological tumor type, tumor location, number of involved lymph nodes, depth of invasion, sex and stage was established. Results: A total of 50 tumor specimens and an equal number of biopsies were evaluated for Ki-67 overexpression. Ten tumors (20%) presented a Ki-67 < 50%, 13 tumors (26%) showed a Ki-67 >50% < 74%, and 27 tumors (54%) had a Ki-67 > 75%. It was found that tumors with a low Ki-67 expression have a more proximal location, the histological type was either mixed or diffuse, tend to invade superficially the gastric wall, they don’t have metastases in distal lymph nodes, and are diagnosed in early stages of the disease. Conclusions: In this study, the Ki-67 overexpression showed prognostic value in both diffuse and intestinal type gastric cancer patients.

scholarly journals Triage of LSIL/ASC-US with p16/Ki-67 dual staining and human papillomavirus testing: a 2-year prospective study

Cytopathology ◽  
2016 ◽  
Vol 27 (4) ◽  
pp. 269-276 ◽  
Author(s):  
C. White ◽  
S. Bakhiet ◽  
M. Bates ◽  
H. Keegan ◽  
L. Pilkington ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Prospective Study ◽  
Ki 67 ◽  
Human Papillomavirus Testing ◽  
Dual Staining

scholarly journals Correlation of survivin, p53 and Ki-67 in laryngeal cancer Hep-2 cell proliferation and invasion

2015 ◽  
Vol 8 (8) ◽  
pp. 636-642 ◽  
Author(s):  
Shi-Geng Pei ◽  
Ju-Xiang Wang ◽  
Xue-Ling Wang ◽  
Qing-Jun Zhang ◽  
Hong Zhang
Keyword(s):  
Cell Proliferation ◽  
Laryngeal Cancer ◽  
Ki 67 ◽  
Proliferation And Invasion

scholarly journals Evaluation of HPV-Related Biomarkers in Anal Cytological Samples from HIV-Uninfected and HIV-Infected MSM

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 888
Author(s):  
Francesca Rollo ◽  
Alessandra Latini ◽  
Massimo Giuliani ◽  
Amalia Giglio ◽  
Maria Gabriella Donà ◽  
...  
Keyword(s):  
Linear Array ◽  
Anal Carcinoma ◽  
High Grade ◽  
Ki 67 ◽  
Hpv Dna ◽  
Cytological Abnormalities ◽  
Sex With Men ◽  
Anal Cancer Screening ◽  
Intraepithelial Lesions ◽  
Dual Staining

Men who have sex with men (MSM) harbor the highest risk for anal carcinoma, mainly caused by Human Papillomavirus (HPV). The use of HPV-related biomarkers in the screening for this neoplasia is still debated. We assessed the association between high-risk (hr)HPV DNA, HPV16/18 DNA, hrHPV E6/E7 mRNA, and p16/Ki-67 with cytological abnormalities (any grade) and high-grade intraepithelial lesions (HSIL) in HIV-uninfected and HIV-infected MSM. Overall, 150 cytological samples in PreservCyt (Hologic), with a negative to HSIL report, were analyzed for hrHPV DNA, hrHPV E6/E7 mRNA, and p16/Ki-67 using the Linear Array (Roche), Aptima (Hologic), and CINtec® PLUS (Roche) assays. In HIV-infected MSM, positivity for all the biomarkers significantly increased with the cytological grade. In both populations, the association of hrHPV E6/E7 mRNA and p16/Ki-67 positivity with HPV16 did not differ significantly compared to hrHPVs other than HPV16. In HIV-uninfected MSM, the odds of having an HSIL increased approximately six times for the p16/Ki-67 positive cases. In HIV-infected individuals, all the biomarkers showed a significant association with HSIL, except for hrHPV DNA, with the strongest association observed for p16/Ki-67. The odds of HSIL increased almost 21 times in those positive for this biomarker. Our results encourage further investigation on the use of p16/Ki-67 dual staining in anal cancer screening for HIV-uninfected and HIV-infected MSM.

scholarly journals Analysis of expression of molecular marker KI-67 in patients with laryngeal cancer for regional metastases and recurrences

2020 ◽  
pp. 37-44
Author(s):  
Alexander Kovtunenko ◽  
Sergey Tymchuk ◽  
Bogdan Shportko
Keyword(s):  
Molecular Markers ◽  
Molecular Marker ◽  
Laryngeal Cancer ◽  
Squamous Cell ◽  
Proliferative Activity ◽  
Tumor Metastasis ◽  
Age Dependence ◽  
Ki 67 ◽  
Cancer Metastases ◽  
Regional Metastases

Background: Laryngeal cancer is a common localization of squamous cell carcinoma of the head and neck. The main factor influencing the survival of patients is the presence of regional metastases. The malignant degeneration of a cell is always accompanied by disruption of the cell cycle, in particular a sharp increase in proliferative activity. The molecular marker Ki-67 is used to investigate the proliferative activity of tumor cells. Aim of the study: To study the informative content of immunohistochemical investigation and to determine the expression of the molecular marker Ki-67 in patients with laryngeal cancer, prior to the prediction of regional metastasis and relapse. Materials and Methods: 70 patients with cancer, stage III-IV (T3-4N0-3M0) clinical group II were under observation. Of these, 39 patients from the main group were with regional laryngeal cancer metastases, and 31 patients in the comparison group – without revealed regional metastases. Patients' age ranged from 33 to 74 years. The average age was 61.9 years. Male patients made up the absolute majority of 99% No = 69. All patients were histologically diagnosed with squamous cell carcinoma. Monoclonal antibodies to Ki67 (TermoScientific, USA) were used as the primary molecular markers. The proliferation index (PI) was rated as low in the range of 0-30% and as high in more than 30% of positive cells with an intranuclear label. Results: The study of molecular markers Ki-67 showed its expression at 69 [60.2; 77.5]% (Me [25%; 75%]) for the squamous cell laryngeal cancer group, without metastases – 60 [29; 70.5]%, for the group with metastases – 72 [67; 80]% (P <0.001). In statistical data processing, the AUC value (0.777) was set for Ki-67 for metastasis and showed an increased degree of expression for laryngeal cancer metastasis. Ki-67 has a good diagnostic value and can be used as an additional method for predicting regional laryngeal cancer metastases. However the practical use of this marker as a prognostic one for determining the volume of surgery on the lymphatic structures of the neck requires further investigation. It should be noted that this study did not reveal the link of Ki-67 AUC (0.588) with the degree of differentiation of laryngeal cancer and its recurrence, but more in-depth study of molecular markers in the future may reveal other patterns of tumor growth and be used in the prognosis and personalization of treatment. Conclusion: Expression of Ki-67 allowed to accurately predict tumor metastasis. A 1% increase in Ki-67 expression resulted in an 8.3% increase in the chance of tumor metastasis (95% CI - 1.043-1.142), and the age dependence on metastasis is not reliable. Determination of expression of Ki-67 does not accurately predict the possibility of recurrence of laryngeal cancer (p=0.27; AUC (95% CI) – 0.588 (0.414-0.763)) herewith age dependence of chance of a recurrence was not revealed.

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