scholarly journals USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINS

2018 ◽  
Vol 11 (1) ◽  
pp. 64-69
Author(s):  
T. S. Kalandyrets ◽  
Keyword(s):  
Pichia Anomala ◽  
Yeast Strains ◽  
Rhodotorula Gracilis

Infl uence of Pesticides on Yeasts Colonizing Leaves

2011 ◽  
Vol 66 (11-12) ◽  
pp. 588-594 ◽  
Author(s):  
Renata Vadkertiová ◽  
Elena Sláviková
Keyword(s):  
Rhodotorula Glutinis ◽  
Fruit Trees ◽  
Pichia Anomala ◽  
Cryptococcus Laurentii ◽  
Forest Trees ◽  
Rhodotorula Mucilaginosa ◽  
Cryptococcus Albidus ◽  
Tree Leaves ◽  
Yeast Strains ◽  
Resistant Strains

The effect of nine different pesticides on the growth of yeasts isolated from the leaves of fruit and forest trees was investigated. Four insecticides (with the active ingredients: thiacloprid, deltamethrin, lambdacyhalothrin, and thiamethoxam) and five fungicides (with the effective substances: bitertanol, kresoxim-methyl, mancozeb, trifl oxystrobin, and cupric oxychloride) were tested. The concentrations of chemicals were those recommended by the manufacturers for the spraying of trees. The yeast strains isolated from the leaves of fruit trees were not sensitive to any of the insecticides. The majority of yeast strains isolated from the leaves of forest trees were either not sensitive or only to a small extent. While Rhodotorula mucilaginosa and Pichia anomala were not affected by any insecticide, the strains of Cryptococcus laurentii and Rhodotorula glutinis showed the highest sensitivity. The effects of fungicides on the growth of isolated yeasts were more substantial. The fungicide DithaneⓇ DG (mancozeb) completely inhibited the growth of all yeasts. All strains isolated from fruit tree leaves were more resistant to the tested fungicides than those isolated from the leaves of forest trees. The most resistant strains from the leaves of fruit trees belonged to the species Metschnikowia pulcherrima, Pichia anomala, and Saccharomyces cerevisiae, whereas Cryptococcus albidus and C. laurentii, originating from the leaves of forest trees, showed the highest sensitivity to fungicides

scholarly journals Pichia myanmarensis sp. nov., a novel cation-tolerant yeast isolated from palm sugar in Myanmar

2005 ◽  
Vol 55 (3) ◽  
pp. 1379-1382 ◽  
Author(s):  
Yuka Nagatsuka ◽  
Hiroko Kawasaki ◽  
Tatsuji Seki
Keyword(s):  
Dna Hybridization ◽  
The Other ◽  
Pichia Anomala ◽  
Hybridization Data ◽  
Halotolerant Yeast ◽  
Yeast Strains ◽  
D2 Domain ◽  
Palm Sugar ◽  
Polyphasic Characterization ◽  
18S Rdna Sequence

Four halotolerant yeast strains, M21T, M34-1, HS054 and D41, were isolated from various foods in South-East Asia. These isolates were most closely related to Pichia anomala, with which each strain had from zero to two differences in the 26S rDNA D1/D2 domain nucleotide sequence; for this reason, they were thought to be the same as, or sister species of, P. anomala. Of the four yeast isolates, only one strain, M21T, had an 18S rDNA sequence that differed from those of P. anomala IFO 10213T and the other three isolates, having 20 substitutions and two gaps. Strain M21T showed lower cation (Li+) tolerance (⩽0·3 M LiCl) than P. anomala IFO 10213T or the other three strains (⩽0·5 M LiCl). Furthermore, the DNA–DNA hybridization data indicated that M21T was clearly distinct from P. anomala IFO 10213T and the other three isolates. The ability of strain M21T to assimilate d-arabinose distinguished it from P. anomala IFO 10213T and the other three isolates; it also differed in that it was able to grow at 37 and 40 °C. Strain M21T grew by multilateral budding, produced persistent asci, in which between one and four hat-shaped ascospores were formed, and contained ubiquinone Q-7. On the basis of this polyphasic characterization, strain M21T represents a novel species within the Q-7-containing group of the genus Pichia, for which the name Pichia myanmarensis is proposed. The type strain is M21T (=NBRC 11090T=JCM 12922T=CBS 9786T).

scholarly journals Inhibition of bacteria contaminating alcoholic fermentations by killer yeasts

2010 ◽  
Vol 53 (5) ◽  
pp. 1043-1050 ◽  
Author(s):  
Maria Cristina Meneghin ◽  
Vanda Renata Reis ◽  
Sandra Regina Ceccato-Antonini
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Alcoholic Fermentation ◽  
Pichia Anomala ◽  
Killer Activity ◽  
Candida Sp ◽  
Gram Positive Bacteria ◽  
Crude Toxin ◽  
Yeast Strains ◽  
In Vitro Antibacterial Activity

The aim of this work was to study the in vitro antibacterial activity possessed by killer yeast strains against bacteria contaminating alcoholic fermentation (Bacillus subtilis, Lactobacillus plantarum, Lactobacillus fermentum and Leuconostoc mesenteroides), in cell X cell and cell X crude toxin preparations. The bacteria were not inhibited by any S. cerevisiae killer strains (5 out of 11). The inhibition caused by two crude toxin preparations (Trichosporon figueirae and Candida sp) against L. plantarum was surprisingly high but not in the same extent for B. subtilis, especially with three killer strains (Candida glabrata, Pichia anomala and Candida sp). L. mesenteroides and L. fermentum strains were neither inhibited in cell X cell nor crude toxin X cell tests. The results suggested that killer activity of yeasts might operate over bacteria and it could be used for the biocontrol of contaminating bacteria from alcoholic fermentation if additional tests on toxin application in fermentation shown to be successful. A wider panel of S. cerevisiae killer strains should be used to confirm that they were really unable to control the growth of these Gram-positive bacteria.

scholarly journals Isolation of Yeasts from Various Food Products and Detection of Killer Toxin Activity In vitro

2010 ◽  
Vol 2 (2) ◽  
pp. 407-411 ◽  
Author(s):  
T. Dubash ◽  
S. Gupta ◽  
P. Y. Prakash ◽  
I. Bairy
Keyword(s):  
Cryptococcus Neoformans ◽  
Killer Toxin ◽  
Food Products ◽  
Selective Advantage ◽  
Geotrichum Candidum ◽  
Pichia Anomala ◽  
Pathogenic Yeast ◽  
Yeast Strains ◽  
Candida Pintolopesii

Yeasts produce killer toxin, which gives them a selective advantage against susceptible yeasts. In this study we isolated 8 various non-pathogenic yeasts from 12 different food products and detected killer toxin activity in them against a clinical isolate of Cryptococcus neoformans by chloroform exposure method. The experiment was performed in two sets that had 3 replicates each. The first set plates and the second set plates were exposed to chloroform once and twice respectively. Among the 8 yeasts, 5 yeasts namely Sachharomyces cerevisiae, Candida pintolopesii, Candida tropicalis, Pichia anomala and Dekkera spp. showed significant amount of killer toxin activity against Cryptococcus neoformans in all the replicates, followed by candida parapsilosis and Trichosporon asahii, which showed killer toxin activity only in two of the three replicates. Geotrichum candidum showed no killer toxin activity. Killer toxin activity was observed in vitro in non-pathogenic yeast strains against medically important Cryptococcus neoformans.  Keywords: Yeasts; Killer toxin; Chloroform exposure. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i2.4159               J. Sci. Res. 2 (2), 407-411 (2010) 

Biosorption of Nickel by Yeasts in an Osmotically Unsuitable Environment

2008 ◽  
Vol 63 (11-12) ◽  
pp. 873-878 ◽  
Author(s):  
Emilia Breierová ◽  
Milan Čertík ◽  
Annamaria Kovárová ◽  
Tomaš Gregor
Keyword(s):  
Heavy Metal ◽  
Metal Ions ◽  
Heavy Metal Ions ◽  
Stress Conditions ◽  
Pichia Anomala ◽  
Adaptation To Stress ◽  
Candida Maltosa ◽  
Sensitive Species ◽  
Nickel Ions ◽  
Yeast Strains

Abstract The tolerance, sorption of nickel(II) ions, and changes in the production and composition of exopolymers of eight yeast strains grown under nickel presence with/without NaCl were studied. Strains of Pichia anomala and Candida maltosa known as the most resistant yeasts against nickel tolerated up to 3 mm Ni2+. NaCl addition decreased both the resistance of the yeast strains toward nickel ions and the sorption of metal ions into cells. All yeasts absorbed nickel predominantly into exopolymers (glycoproteins) and on the surface of cells. However, while the amount of polysaccharide moieties of exoglycoproteins of most of the resistant yeasts was induced by stress conditions, the ratio polysaccharide/protein in the exopolymers remained unchanged in the sensitive species Cystofilobasidium. The exopolymer composition might play a key role in yeast adaptation to stress conditions caused by heavy metal ions.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

Characteristics of ethanol yeast strains in production conditions under higher mash solids concentration conditions

2019 ◽  
Vol 7 (12) ◽  
pp. 121-127
Author(s):  
Taras Lysak ◽  
◽  
Serhii Oliinichuk ◽  
Olha Koval ◽  
◽  
...  
Keyword(s):  
Yeast Strains ◽  
Solids Concentration ◽  
Production Conditions

The benefits of applied research: 37 years of discoveries, adaptations and solutions

2015 ◽  
pp. 209-216 ◽  
Author(s):  
Eduardo P. Borges ◽  
Mário L. Lopes ◽  
Claudemir Bernardino ◽  
Alexandre Godoy ◽  
Fernando E. Ré ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Statistical Analysis ◽  
Applied Research ◽  
Sugar Production ◽  
Industrial Fermentation ◽  
Yeast Strains ◽  
Aconitic Acid ◽  
Key Factor ◽  
Cane Quality ◽  
Dry Substance

The authors’ work started in fermentation in 1977 and in the 1980’s into sugar production and cane quality. Statistical analysis was a key factor for the success of improving yield in ethanol and sugar production as well as cane quality. Adaption of methods for industrial laboratories also was very important in relation to yield and in reduction of sugar losses in the factory. Methodologies to measure sugar losses occurring through degradation in the factory (evaporation) using ion chromatography and dry substance content with a digital density meter were adapted. The fermentation yield improved from 75% in 1977 to 92% in 2014, which was possible by adapting methods for live bacterial counting within 20 min, and by controlling contamination using antimicrobial products through research in the laboratory and the industry. Since 1990 yeasts for industrial fermentation were selected by karyotyping analysis of the nuclear chromosomes and in the last seven years based on mitochondrial DNA. The last technique made the “Process Driven Selection” possible, i.e. one or several yeast strains which fit each distillery. Floc formation in carbonated beverages is not only due to the Indicator Value (discovery by SPRI research group) but also to aconitic acid and calcium under Brazilian conditions.

Treatment of wastewater from oil manufacturing plant by yeasts

1996 ◽  
Vol 34 (11) ◽  
pp. 51-58 ◽  
Author(s):  
K. Chigusa ◽  
T. Hasegawa ◽  
N. Yamamoto ◽  
Y. Watanabe
Keyword(s):  
Pilot Plant ◽  
Removal Rate ◽  
Oil Removal ◽  
Oxidation Activity ◽  
Single Strain ◽  
Manufacturing Plants ◽  
Yeast Strains ◽  
Hexane Extracts ◽  
One Year ◽  
Raw Wastewater

Nine strains of yeasts capable of decomposing oil were isolated in order to directly treat wastewater from oil manufacturing plants with no pretreatment. The oil decomposing ability of these yeast strains was evaluated in terms of lipase activity and β-oxidation activity. Since the mixture of the isolated yeasts was superior to any single strain in the oil removal rate, a pilot plant utilizing the mixed strains was operated at the soybean oil factory. Following a one year pilot plant operation, it was found that 10,000 mgℓ−1 of hexane extracts in the raw wastewater could be reduced by yeast treatment to a concentration of about 100 mgℓ−1. This concentration was further treated by the activated sludge process to 2 mgℓ−1. The dominant yeasts in the pilot plant were found to form mycelial or pseudomycelial pellets and have low fermenting ability.

Sign in / Sign up

Export Citation Format

Share Document