scholarly journals Sex identification of different species of wild birds using a single universal protocol to the bird sexing method based on gene polymorphism

2020 ◽  
Vol 11 (3) ◽  
pp. 399-404
Author(s):  
S. V. Rudaуа ◽  
О. О. Katerynych ◽  
M. V. Drahulian ◽  
A. B. Chaplygina ◽  
O. Y. Pakhomov
Keyword(s):  
Gene Polymorphism ◽  
Sex Determination ◽  
Bird Species ◽  
Wild Birds ◽  
White Stork ◽  
Herring Gull ◽  
Interspecific Differences ◽  
Unified Protocol ◽  
Common Pheasant ◽  
Universal Protocol

This article presents an elaboration of the protocol for the method of sexing wild birds based on the polymorphism of the CHD gene using P2/P8 primer for Common Pheasant – Phasianus colchicus (Linnaeus, 1758; Galliformes, Phasianidae); Silver Lofur or Silver Pheasant – Lophura nycthemera (Linnaeus, 1758; Galliformes, Phasianidae), Budgerigar – Melopsittacus undulatus (Shaw, 1805; Psittaciformes, Psittacidae), Herring Gull – Larus argentatus (Pontoppidan, 1763; Charadriiformes, Laridae), and White Stork – Ciconia ciconia (Linnaeus, 1758; Ciconiiformes, Ciconiidae). Blood samples were taken from Common Pheasant, Silver Pheasant and White Stork using the “drop of blood on paper” method. For the Budgerigar and the Herring Gull, DNA was isolated from the feather follicle. To isolate DNA, a commercial NeoPrep 100 DNA reagent kit (Neogen, Ukraine) was used. Primers P2/P8 were used for PCR; PCR was performed using GenPac PCR Core reagents (Neogen, Ukraine). We selected the optimal amount of Tag polymerase, the amount of DNA and primers and, according to the amount of reagents, set acceptable amplification modes and electrophoresis agarose gel percentage. Prior to PCR, additional DNA gel electrophoresis purification is proposed, which increases the percentage of positive sex determination results. It was found that the ideal mixture for the 5 bird species was an amplification mixture (total volume 20 µL, containing 1 U Tag polymerase, 100 ng DNA and 0.6 µM of each primer). The amplified CHD-Z fragment of Common and Silver pheasants is of ~340 n. p., CHD-W ~360 n. p. Herring Gull and Budgerigar have ~350 n. p. of CHD-Z length, and ~400 n. p. of CHD-W length, White Stork has its CHD-Z of ~ 370 n. p. long. It is advisable to investigate the genome of the experimental bird species using horizontal electrophoresis in agar’s gel with the concentration of 5%, which makes it possible to clearly visualize the female genotype. The universal protocol of the method of sex determination based on polymorphism of the CHD gene for the 5 studied bird species is described. These results of the study led to the conclusion that for the simultaneous sexing of several species of birds, it is advisable to develop a unified protocol for determining the status of the CHD gene, with the aim of clarifying the gender, as well as new approaches in ornithology and ecology aimed at determining interspecific differences associated with gene polymorphism. Identification of differences in fragment sizes may be useful for identifying the species in cases when birds form mixed pairings for taxonomic and phylogenetic comparisons.

scholarly journals Abundance, diversity and daily activity of terrestrial mammal and bird species in disturbed and undisturbed limestone habitats using camera trapping, Central Thailand

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
MANANYA PLA-ARD ◽  
WIPAVEE HOONHEANG ◽  
BUNYATHIPORN KAEWDEE ◽  
TARAPON PANGANTA ◽  
KHWANRUTAI CHARASPET ◽  
...  
Keyword(s):  
Daily Activity ◽  
Bird Species ◽  
Wild Birds ◽  
Camera Trapping ◽  
Mountainous Area ◽  
Mining Activities ◽  
Natural Habitats ◽  
Terrestrial Mammal ◽  
Significant Difference ◽  
Central Thailand

Abstract: Pla-ard M, Hoonheang W, Kaewdee B, Panganta T, Charaspet K, Khoiesri N, Paansri P, Kanka P, Chanachai Y, Thongbanthum J, Bangthong P, Sukmasuang R. 2021. Abundance, diversity and daily activity of terrestrial mammal and bird species in disturbed and undisturbed limestone habitats using camera trapping, Central Thailand. Biodiversitas 22: 3620-3631.  This study on the abundance, diversity and daily activity of terrestrial mammal and bird species was conducted in the limestone mountainous area of Central Thailand, located on the east of Dong Phaya Yen-Khao Yai forest complex. Camera traps were placed in both habitats disturbed by limestone mining and undisturbed habitat areas. From the study, a total of 38 species of mammals and birds from 27 families in 13 orders were recorded, including 15 species of mammals from 6 orders, 12 families and 23 species of birds from 14 families in 7 orders. Fifteen species of mammals were recorded in the undisturbed area and 11 were recorded in the disturbed area, with the Malayan Pangolin, Small Indian Civet and Grey-bellied Squirrel found in the undisturbed area. However, the number of bird species in the limestone mining area was larger than in the undisturbed area. It was also found that there was no difference in the overall abundance and diversity of mammalian species between disturbed and undisturbed areas, which is not in accordance with the hypothesis. But in the case of wild birds, the relative abundance of wild birds was found to differ significantly between areas. A high number was found in the areas with mining activities, although there was no difference in the diversity index of the two areas. However, it was found that when the combined data was analyzed, there was a significant difference in the daily activity of both mammals and wild birds in both areas. Many rare wildlife species were recorded during this study, for example, the Malayan Pangolin, Serow, Northern Pig-tailed Macaque, Rufous Limestone-babbler, Golden Jackal, Leopard Cat, Large-toothed Ferret Badger, Small Asian Mongoose, Common Palm Civet, Small Indian Civet, Malayan Porcupine. The key measure proposed is to preserve some natural habitats within the areas with mining activities, as wildlife remains in the area.

Absence of sex differences in digit ratio in nestlings of the White Stork Ciconia ciconia, a monomorphic bird species

Bird Study ◽  
2019 ◽  
Vol 66 (4) ◽  
pp. 503-509 ◽  
Author(s):  
Piotr P. Kazimirski ◽  
Mikołaj Kaczmarski ◽  
Magdalena M. Zagalska-Neubauer ◽  
Katarzyna M. Żołnierowicz ◽  
Marcin Tobółka
Keyword(s):  
Sex Differences ◽  
Bird Species ◽  
Digit Ratio ◽  
White Stork ◽  
Ciconia Ciconia

scholarly journals Arthropod Resource Partitioning Among Omnivorous Tanagers (Tangara SPP.) in Western Ecuador

The Auk ◽  
2007 ◽  
Vol 124 (1) ◽  
pp. 197-209 ◽  
Author(s):  
Kazuya Naoki
Keyword(s):  
Resource Use ◽  
Foraging Ecology ◽  
Bird Species ◽  
Sympatric Species ◽  
Factors Affecting ◽  
Use Patterns ◽  
Interspecific Differences ◽  
Fruit Species ◽  
Mixed Species Flocks ◽  
Different Characteristics

Abstract The distribution and abundance of food are primary factors affecting resource-use patterns in birds. Many bird species eat several food types, which may differ in their distribution and overall abundance. I studied foraging ecology of seven sympatric species of Tangara at Mindo, Ecuador, to determine whether the patterns of resource use differed between two food types: arthropods and fruits. Interspecific differences in arthropod-foraging were manifested in the fine segregation of microhabitat preference combined with different habitat use. By contrast, interspecific differences in fruit-foraging were manifested in preferences for different plant genera, often associated with different habitats. No evidence was found for spatial partitioning of the same fruit species. Interspecific overlap in fruit-for- aging was 3× higher than that in arthropod-foraging, and species of Tangara that frequently joined the same mixed-species flocks differed largely in arthropod-foraging but overlapped greatly in fruit-foraging. The differences in patterns between arthropod and fruit-foraging may be explained by the different characteristics of arthropods and fruits as food resources. High sympatry of species of Tangara and other omnivorous tanagers, in general, appears to be maintained not because fruits are abundant, resulting in little competition for them, but because these tanagers specialize on different microhabitats for foraging arthropods. Segregación de Artrópodos como Recurso Alimenticio de Tangaras Omnívoras (Tangara spp.) en el Oeste de Ecuador

scholarly journals A case of beak deformity in the Shiny Cowbird Molothrus bonariensis and a review on beak deformities in wild birds in Brazil

2019 ◽  
Vol 27 (3) ◽  
pp. 212-217
Author(s):  
Keila Nunes Purificação
Keyword(s):  
Literature Review ◽  
Bird Species ◽  
Bird Communities ◽  
Wild Birds ◽  
Mato Grosso ◽  
Molothrus Bonariensis ◽  
East Region ◽  
Beak Deformity

AbstractBeak deformities in wild birds are rarely reported. Here, I described a case of beak deformity in Shiny Cowbird, Molothrus bonariensis, and also provide a review on beak deformities recorded in wild birds in Brazil. In October 2016, I observed a M. bonariensis with a grossly elongated maxilla in the east region of the Mato Grosso state, Brazil. The literature review revealed 60 records of beak deformities in the Brazilian avifauna. The most common types of deformity were crossed maxillae or mandibles (38%), probable accidental injuries (27%), and elongations (15%). Deformities were reported in 35 bird species of 22 families. The most affected species was Ramphastos toco with 21 records. The number of published reports from Brazil was low overall, and are not related as an epizootic episode recorded in some bird communities.

scholarly journals Molecular Characterization and Antimicrobial Susceptibility of C. jejuni Isolates from Italian Wild Bird Populations

Pathogens ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 304 ◽  
Author(s):  
Francesca Marotta ◽  
Anna Janowicz ◽  
Lisa Di Marcantonio ◽  
Claudia Ercole ◽  
Guido Di Donato ◽  
...  
Keyword(s):  
Nalidixic Acid ◽  
Point Mutations ◽  
Bird Species ◽  
Acid Resistance ◽  
Wild Birds ◽  
23S Rrna ◽  
The Novel ◽  
Phenotypic Resistance ◽  
Bird Populations ◽  
Sequence Types

Poultry is considered a major reservoir of human campylobacteriosis. It also been reported that not only poultry, but also wild birds, are capable of carrying C. jejuni, thus demonstrating to be a risk of spreading the bacteria in the environment. To gain insight into the population structure and investigate the antimicrobial resistance genotypes and phenotypes, we analyzed a collection of 135 C. jejuni from 15 species of wild birds in Italy. MLST revealed the presence of 41 sequence types (STs) and 13 clonal complexes (CCs). ST-179 complex and the generalist ST-45 complex were the most prevalent. Core genome MLST revealed that C. jejuni from ST-45 complex clustered according to the bird species, unlike the ST-179 complex which featured 3 different species in the same cluster. Overall we found a moderate prevalence of resistance to tetracycline (12.5%), ciprofloxacin and nalidixic acid (10%). The novel ST isolated from one pigeon showed resistance to all the antibiotics tested. The ST-179 complex (33.3%) was identified with significantly higher nalidixic acid resistance relative to other tested STs. Nine AMR genes (tet(O), cmeA, cmeB, cmeC, cmeR, aad, blaOXA-61, blaOXA-184 and erm(B)) and 23S rRNA and gyrA-associated point mutations were also described, indicating a concordance level between genotypic and phenotypic resistance of 23.3%, 23.4% and of 37.5% for streptomycin, tetracycline and quinolones/fluoroquinolones, respectively. We recommend that particular attention should be given to wild birds as key sentinel animals for the ecosystem contamination surveillance.

scholarly journals Prevalence and Diversity of Avian Influenza Virus Hemagglutinin Sero-Subtypes in Poultry and Wild Birds in Bangladesh

2020 ◽  
Vol 7 (2) ◽  
pp. 73 ◽  
Author(s):  
Mohammad M. Hassan ◽  
Mohamed E. El Zowalaty ◽  
Ariful Islam ◽  
Shahneaz A. Khan ◽  
Md. K. Rahman ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Migratory Birds ◽  
Bird Species ◽  
Wild Birds ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Influenza Virus Hemagglutinin

Highly pathogenic avian influenza H5 viruses have pandemic potential, cause significant economic losses and are of veterinary and public health concerns. This study aimed to investigate the distribution and diversity of hemagglutinin (HA) subtypes of avian influenza virus (AIV) in poultry and wild birds in Bangladesh. We conducted an avian influenza sero-surveillance in wild and domestic birds in wetlands of Chattogram and Sylhet in the winter seasons 2012–2014. We tested serum samples using a competitive enzyme-linked immunosorbent assay (c-ELISA), and randomly selected positive serum samples (170 of 942) were tested using hemagglutination inhibition (HI) to detect antibodies against the 16 different HA sero-subtypes. All AIV sero–subtypes except H7, H11, H14 and H15 were identified in the present study, with H5 and H9 dominating over other subtypes, regardless of the bird species. The diversity of HA sero-subtypes within groups ranged from 3 (in household chickens) to 10 (in migratory birds). The prevalence of the H5 sero-subtype was 76.3% (29/38) in nomadic ducks, 71.4% (5/7) in household chicken, 66.7% (24/36) in resident wild birds, 65.9% (27/41) in migratory birds and 61.7% (29/47) in household ducks. Moreover, the H9 sero-subtype was common in migratory birds (56%; 23/41), followed by 38.3% (18/47) in household ducks, 36.8% (14/38) in nomadic ducks, 30.6% (11/66) in resident wild birds and 28.5% (2/7) in household chickens. H1, H4 and H6 sero-subtypes were the most common sero-subtypes (80%; 8/10, 70%; 7/10 and 70%; 7/10, respectively) in migratory birds in 2012, H9 in resident wild birds (83.3%; 5/6) and H2 in nomadic ducks (73.9%; 17/23) in 2013, and the H5 sero-subtype in all types of birds (50% to 100%) in 2014. The present study demonstrates that a high diversity of HA subtypes circulated in diverse bird species in Bangladesh, and this broad range of AIV hosts may increase the probability of AIVs’ reassortment and may enhance the emergence of novel AIV strains. A continued surveillance for AIV at targeted domestic–wild bird interfaces is recommended to understand the ecology and evolution of AIVs.

Epidemiological perspectives on West Nile virus surveillance in wild birds in Great Britain

2012 ◽  
Vol 141 (6) ◽  
pp. 1134-1142 ◽  
Author(s):  
V. A. BRUGMAN ◽  
D. L. HORTON ◽  
L. P. PHIPPS ◽  
N. JOHNSON ◽  
A. J. C. COOK ◽  
...  
Keyword(s):  
Great Britain ◽  
West Nile Virus ◽  
Bird Species ◽  
Wild Birds ◽  
West Nile ◽  
Peak Period ◽  
Dead Bird ◽  
Virus Surveillance ◽  
Vector Activity ◽  
Geographical Expansion

SUMMARYWest Nile virus (WNV) is a zoonotic arthropod-borne pathogen with continued geographical expansion in Europe. We present and evaluate data on the temporal, spatial and bird species focus of the WNV surveillance programme in dead wild birds in Great Britain (2002–2009). During this period all bird samples tested negative for WNV. Eighty-two per cent of the 2072 submissions occurred during the peak period of vector activity with 53% tested during April–July before human and equine infection would be expected. Samples were received from every county, but there was significant geographical clustering (nearest neighbour index=0·23, P<0·001). Over 240 species were represented, with surveillance more likely to detect WNV in resident bird species (92% of submissions) than migrants (8%). Evidence indicates that widespread avian mortality is not generally a reported feature of WNV in Europe and hence additional activities other than dead bird surveillance may maximize the ability to detect WNV circulation before the onset of human and equine infections.

Sex determination in ostrich (Struthio camelus) using DNA markers

2010 ◽  
Vol 90 (3) ◽  
pp. 357-360 ◽  
Author(s):  
M. Alipanah ◽  
A. Torkamanzehi ◽  
H. Taghavi
Keyword(s):  
Polymerase Chain Reaction ◽  
Sexual Dimorphism ◽  
Sex Determination ◽  
Sex Chromosome ◽  
Molecular Genetic ◽  
Bird Species ◽  
Rapid Identification ◽  
Chain Reaction ◽  
Struthio Camelus ◽  
Polymerase Chain

Production of bird species such as ostrich (Struthio camelus) has been gaining increasing importance in Iran as well as many other countries. Ostrich, similar to many other species of birds, lacks sexual dimorphism, making it difficult to differentiate between males and females, especially at an early age, which can be problematic in breeding programs. Recently developed molecular genetic methods that utilize polymerase chain reaction (PCR) based techniques can facilitate rapid identification of the bird’s sex in these species using a DNA sample, which can be easily extracted from blood or feather pulps. We successfully applied a PCR-based RFLP technique and sex chromosome primers for sex determination in a sample of 30 Ostrich chicks using DNA extracted from blood and feather pulps. Both DNA samples (blood and feather pulps) provided useful results. However, using feather pulps from 1-day-old chicks can provide an easy and inexpensive method for sex determination in ostrich. Key words: Ostrich (struthio camelus), sex determination, sexual dimorphism, polymerase chain reaction, RFLP

scholarly journals Reassortant low-pathogenic avian influenza H5N2 viruses in African wild birds

2011 ◽  
Vol 92 (5) ◽  
pp. 1172-1183 ◽  
Author(s):  
Chantal J. Snoeck ◽  
Adeniyi T. Adeyanju ◽  
Sébastien De Landtsheer ◽  
Ulf Ottosson ◽  
Shiiwua Manu ◽  
...  
Keyword(s):  
Avian Influenza ◽  
Migratory Birds ◽  
Phylogenetic Analyses ◽  
Bird Species ◽  
Sister Group ◽  
Wild Birds ◽  
Pathogenic Avian Influenza ◽  
Ns Gene ◽  
Domestic Birds ◽  
Low Pathogenic Avian Influenza

To investigate the presence and persistence of avian influenza virus in African birds, we monitored avian influenza in wild and domestic birds in two different regions in Nigeria. We found low-pathogenic avian influenza (LPAI) H5N2 viruses in three spur-winged geese (Plectropterus gambensis) in the Hadejia–Nguru wetlands. Phylogenetic analyses revealed that all of the genes, except the non-structural (NS) genes, of the LPAI H5N2 viruses were more closely related to genes recently found in wild and domestic birds in Europe. The NS genes formed a sister group to South African and Zambian NS genes. This suggested that the Nigerian LPAI H5N2 viruses found in wild birds were reassortants exhibiting an NS gene that circulated for at least 7 years in African birds and is part of the African influenza gene pool, and genes that were more recently introduced into Africa from Eurasia, most probably by intercontinental migratory birds. Interestingly the haemagglutinin and neuraminidase genes formed a sister branch to highly pathogenic avian influenza (HPAI) H5N2 strains found in the same wild bird species in the same wetland only 1 year earlier. However, they were not the closest known relatives of each other, suggesting that their presence in the wetland resulted from two separate introductions. The presence of LPAI H5N2 in wild birds in the Hadejia–Nguru wetlands, where wild birds and poultry occasionally mix, provides ample opportunity for infection across species boundaries, with the potential risk of generating HPAI viruses after extensive circulation in poultry.

scholarly journals Urban trees to attract wild birds in a tropical urban residential complex in Sentul, West Java, Indonesia

2021 ◽  
Vol 918 (1) ◽  
pp. 012003
Author(s):  
A Mardiastuti
Keyword(s):  
Bird Species ◽  
Acacia Mangium ◽  
Wild Birds ◽  
Urban Trees ◽  
Calliandra Calothyrsus ◽  
Residential Areas ◽  
West Java ◽  
Insects As Food ◽  
Nest Materials ◽  
Samanea Saman

Abstract In an urban residential complex, in addition to providing environmental services, trees also attract wild birds. The objective of this research was to identify trees planted in tropical urban residential areas that can attract wild birds. The research was conducted in Sentul City Residential Complex, Bogor, West Java, by observing wild birds that visit certain trees as part of their habitat components. Trees provide nectar, fruits, seeds, and insects as food for birds and nest materials. Trees also have important functions as birds’ cover and nesting sites. There were 103 tree species, all basically supporting the life of 51 bird species found in the study area. Examples of nectar producer trees were Spathodea campanulata, Callistemon citrinus, Erythrina crista-galli; fruit producers were Muntingia calabura, Syzygium polyanthum, Ficus benjamina; seeds producers were Leucaena leucocephala, Paraserianthes falcataria. Many trees also attracted insects for bird food, including Acacia mangium, Samanea saman. Conifers and small twigs and leaf petioles/rachis of many legumes, for example, Delonix regia, Calliandra calothyrsus) can be used as nest materials. Careful selection of trees could attract many wild birds to increase the environmental quality of the residential areas.

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