scholarly journals Effectiveness of photodynamic therapy and sodium hypochlorite on root canal system infected with Enterococcus faecalis – An in vitro study

2016 ◽  
Vol 31 (3) ◽  
pp. 114
Author(s):  
Matheus Albino Souza ◽  
Liviu Steier ◽  
Giampiero Rossi-Fedele ◽  
Valdir Barth Júnior ◽  
Silvia Dias De Oliveira ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Negative Control ◽  
Canal Wall ◽  
Canal System ◽  
Group 4 ◽  
Root Canal System

Objective: The purpose of this study was to evaluate, in vitro, the effectiveness of photodynamic therapy and sodium hypochlorite over root canal system infected with Enterococcus faecalis.Methods: The root canals of 45 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (G1 – n=5; G2-G5 – n=10) according to the protocol of decontamination: G1 (negative control) – no procedure was performed; G2 – distilled water; G3 –  hotodynamictherapy (PDT); G4 – 2.5% sodium hypochlorite (NaOCl); and G5 – 2.5% NaOCl+PDT. Scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments. The assessment was made by images of each third at 5000x magnification for the canal wall and 10000x for the exposed tubule area. The presence of bacteria was scored by position ranks from 1 to 45, where the higher the value, the cleanliness of the sample. Data were subjected to one-way ANOVA followed by post-hoc Tukey test (α=0.05).Results: Group 4 (2.5% NaOCl) and 5 (2.5% NaOCl + PDT) had the highest mean of position ranks for all thirds of the root canal in the canal wall, which was statistically different from groups 1, 2 and 3 (p<0.05). Group 3 (PDT), group 4 (2.5% NaOCl) and 5 (2.5% NaOCl+PDT) had the highest mean of position ranks in the exposed tubule area, which was statistically different from groups 1 and 2 (p<0.05).Conclusion: the association of 2.5% sodium hypochlorite + photodynamic therapy can be considered an effective protocol for the elimination of Enterococcus faecalis.

Influence of ultrasonic activation on photodynamic therapy over root canal system infected with Enterococcus faecalis – an in vitro study

2014 ◽  
Vol 11 (4) ◽  
pp. 472-478 ◽  
Author(s):  
Guilherme Cavagnoli Ghinzelli ◽  
Matheus Albino Souza ◽  
Doglas Cecchin ◽  
Ana Paula Farina ◽  
José Antônio Poli de Figueiredo
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Canal System ◽  
Ultrasonic Activation ◽  
Root Canal System

scholarly journals Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study

2017 ◽  
Vol 6 (2) ◽  
pp. 65-67
Author(s):  
Neha Saini ◽  
Abhinav C Singhal ◽  
Aditi S Jain ◽  
Jayesh Tiwari ◽  
Pratik Surana
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Root Canal ◽  
Antibacterial Efficacy ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Canal System ◽  
Root Canal System ◽  
Tea Extract

ABSTRACT Introduction The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity. Aims and objectives To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis. Materials and methods Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm. Results Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract. Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala. How to cite this article Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.

scholarly journals Effectiveness of high-power diode laser associated to chemical auxiliary substance on decontamination of root canal system infected with Enterococcus faecalis – an in vitro study

2014 ◽  
Vol 18 (3) ◽  
Author(s):  
Matheus Albino Souza ◽  
Tiago Lange dos Santos ◽  
Alessandra Kuhn Dall’Magro ◽  
Fabiana Vieira Vier-Peliser ◽  
Luciana Ruschel dos Santos ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
High Power ◽  
Root Canal ◽  
In Vitro Study ◽  
Canal System ◽  
Auxiliary Substance ◽  
Root Canal System ◽  
Power Diode ◽  
High Power Diode Laser

Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy showed Reliable Efficacy against the Most Resistant Endodontic Bacteria Enterococcus faecalis

2012 ◽  
Vol 9 (2) ◽  
pp. 1-4
Author(s):  
Ibrahim Khalil ◽  
KM Mohidul Islam ◽  
Md Zahid Hossain ◽  
Arup Kumar Shah ◽  
Akashlynn Badruddoza ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Tissue Repair ◽  
Treatment Success ◽  
Canal System ◽  
Cross Sectional ◽  
Research Hypothesis ◽  
Root Canal System

Sterilization of bacteria in root canal system is one of the prominent problems. Some bacteria could remain in root canal even after using conventional medicaments. Evidences suggested that Enterococcus faecalis (E. faecalis) caused substantial root canal infections. So, elimination of such organism is important to achieve treatment success. Research hypothesis: Sterilization of root canal with Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy is thought to be more effective against E. faecalis comparing to single antibiotic. Methods: In-vitro cross-sectional with interventional type of study to observe the zone of inhibitions by E. faecalis culture using LSTR- 3mix (metronidazole, ciprofloxacin and minocycline) and calcium hydroxide (control). Results: The results strongly recommended that LSTR-3mix sufficiently able to inhibit E. faecalis growth. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12311 City Dental College J. Volume-9, Number-2, July-2012

scholarly journals Comparative Evaluation of the Efficacy of Irrigation Delivery Systems on Enterococcus faecalis

2018 ◽  
Vol 25 (3) ◽  
Author(s):  
Raksha Bhat ◽  
◽  
Preethesh Shetty ◽  
Mithra N. Hegde ◽  
◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Delivery Systems ◽  
Semiconductor Diode ◽  
Health Administration ◽  
Canal System ◽  
Group I ◽  
Root Canal System

Attaining a sterile root canal system is important since microorganisms can cause persistent inflammation in the periradicular tissues. Objectives: This study sought to evaluate the efficacy of two irrigation delivery systems; the EndoVac and semiconductor diode laser irradiation with 5.25% sodium hypochlorite and 2% chlorhexidine. Methods: Fifty teeth were disinfected according to the Occupational Safety and Health Administration regulations. The teeth were then instrumented, followed by inoculation with bacterial strains of Enterococcus faecalis (ATCC 29212). The teeth were randomly assigned to five groups, Group I: Control group; Group II: Teeth irrigated with 5.25% NaOCl + Endovac; Group III: Teeth irrigated with 2% Chlorhexidine + Endovac; Group IV: Teeth irrigated with 5.25% NaOCl + laser irradiation; and Group V: Teeth irrigated with 2% Chlorhexidine laser irradiation. All samples were incubated on Muller–Hilton media plates for a period of 24 h. We determined the colony-forming units and analyzed them statistically using Fisher’s exact test. Results: Laser irradiation completely disinfected the root canal system. The EndoVac system produced significant disinfection but was comparatively less effective than laser irradiation. Conclusion: Laser irradiation had a significantly greater bactericidal effect than the EndoVac system, used in conjunction with sodium hypochlorite and chlorhexidine.

scholarly journals Assessment of Antimicrobial Activity of Endodontic Sealers on Enterococcus faecalis: An in vitro Study

2012 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Anjali Kaiwar ◽  
Gururaj Nadig ◽  
Jayashree Hegde ◽  
S Lekha
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Filling Material ◽  
Canal System ◽  
Filling Materials ◽  
Root Canal System ◽  
Endodontic Sealers

ABSTRACT Microorganisms invading the root canal system of a tooth may interact with the host tissue and cause pulpo-periapical pathosis. The goals of root canal treatment are to disinfect the root canal system and to prevent subsequent reinfection. The disinfection is attempted with endodontic instruments, irrigants, and medications. Due to the complex canal anatomy, microorganisms can persist even after thorough disinfection regimens are used. After disinfection, the canal is sealed with a root filling material along with sealers. To curtail residual microorganisms, root-filling materials should ideally be bactericidal, as well as biocompatible. In addition, the root filling materials are expected to act as a physical barrier to prevent the leakage of substrate to any residual microorganisms and the ingress of additional microorganisms. The aim of the present study was to assess the antimicrobial activity of various Endodontic sealers on the Enterococcus faecalis. Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. In the changing face of dental care, continued research on E. faecalis and its elimination from the dental apparatus may well define the future of the endodontic specialty. How to cite this article Kaiwar A, Nadig G, Hegde J, Lekha S. Assessment of Antimicrobial Activity of Endodontic Sealers on Enterococcus faecalis: An in vitro Study. World J Dent 2012;3(1):26-31.

Sign in / Sign up

Export Citation Format

Share Document