scholarly journals Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study

2017 ◽  
Vol 6 (2) ◽  
pp. 65-67
Author(s):  
Neha Saini ◽  
Abhinav C Singhal ◽  
Aditi S Jain ◽  
Jayesh Tiwari ◽  
Pratik Surana
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Root Canal ◽  
Antibacterial Efficacy ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Canal System ◽  
Root Canal System ◽  
Tea Extract

ABSTRACT Introduction The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity. Aims and objectives To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis. Materials and methods Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm. Results Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract. Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala. How to cite this article Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.

scholarly journals Effectiveness of high-power diode laser associated to chemical auxiliary substance on decontamination of root canal system infected with Enterococcus faecalis – an in vitro study

2014 ◽  
Vol 18 (3) ◽  
Author(s):  
Matheus Albino Souza ◽  
Tiago Lange dos Santos ◽  
Alessandra Kuhn Dall’Magro ◽  
Fabiana Vieira Vier-Peliser ◽  
Luciana Ruschel dos Santos ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
High Power ◽  
Root Canal ◽  
In Vitro Study ◽  
Canal System ◽  
Auxiliary Substance ◽  
Root Canal System ◽  
Power Diode ◽  
High Power Diode Laser

Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy showed Reliable Efficacy against the Most Resistant Endodontic Bacteria Enterococcus faecalis

2012 ◽  
Vol 9 (2) ◽  
pp. 1-4
Author(s):  
Ibrahim Khalil ◽  
KM Mohidul Islam ◽  
Md Zahid Hossain ◽  
Arup Kumar Shah ◽  
Akashlynn Badruddoza ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Tissue Repair ◽  
Treatment Success ◽  
Canal System ◽  
Cross Sectional ◽  
Research Hypothesis ◽  
Root Canal System

Sterilization of bacteria in root canal system is one of the prominent problems. Some bacteria could remain in root canal even after using conventional medicaments. Evidences suggested that Enterococcus faecalis (E. faecalis) caused substantial root canal infections. So, elimination of such organism is important to achieve treatment success. Research hypothesis: Sterilization of root canal with Lesion Sterilization and Tissue Repair (LSTR)-3mix MP Therapy is thought to be more effective against E. faecalis comparing to single antibiotic. Methods: In-vitro cross-sectional with interventional type of study to observe the zone of inhibitions by E. faecalis culture using LSTR- 3mix (metronidazole, ciprofloxacin and minocycline) and calcium hydroxide (control). Results: The results strongly recommended that LSTR-3mix sufficiently able to inhibit E. faecalis growth. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12311 City Dental College J. Volume-9, Number-2, July-2012

scholarly journals Assessment of Antimicrobial Activity of Endodontic Sealers on Enterococcus faecalis: An in vitro Study

2012 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Anjali Kaiwar ◽  
Gururaj Nadig ◽  
Jayashree Hegde ◽  
S Lekha
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Filling Material ◽  
Canal System ◽  
Filling Materials ◽  
Root Canal System ◽  
Endodontic Sealers

ABSTRACT Microorganisms invading the root canal system of a tooth may interact with the host tissue and cause pulpo-periapical pathosis. The goals of root canal treatment are to disinfect the root canal system and to prevent subsequent reinfection. The disinfection is attempted with endodontic instruments, irrigants, and medications. Due to the complex canal anatomy, microorganisms can persist even after thorough disinfection regimens are used. After disinfection, the canal is sealed with a root filling material along with sealers. To curtail residual microorganisms, root-filling materials should ideally be bactericidal, as well as biocompatible. In addition, the root filling materials are expected to act as a physical barrier to prevent the leakage of substrate to any residual microorganisms and the ingress of additional microorganisms. The aim of the present study was to assess the antimicrobial activity of various Endodontic sealers on the Enterococcus faecalis. Enterococcus faecalis is a microorganism commonly detected in asymptomatic, persistent endodontic infections. In the changing face of dental care, continued research on E. faecalis and its elimination from the dental apparatus may well define the future of the endodontic specialty. How to cite this article Kaiwar A, Nadig G, Hegde J, Lekha S. Assessment of Antimicrobial Activity of Endodontic Sealers on Enterococcus faecalis: An in vitro Study. World J Dent 2012;3(1):26-31.

Comparative Analysis of Protaper and Waveone Systems to Reduce Enterococcus Faecalis from Root Canal System in Primary Molars – An in Vitro Study

2016 ◽  
Vol 40 (2) ◽  
pp. 124-128
Author(s):  
Sérgio Luiz Pinheiro ◽  
Carolina Pessoa ◽  
Josianne Neres da Silva ◽  
Rafael Orro Gonçalves ◽  
Danilo Antonio Duarte ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Wilcoxon Test ◽  
Primary Molars ◽  
Control Group ◽  
Canal System ◽  
Root Canal System ◽  
Group B

Objective: To assess, in vitro, the ability of the ProTaper™ and WaveOne™ systems to reduce Enterococcus faecalis contamination in primary molars. Study design: Sixty roots of primary molars were contaminated with E. faecalis. Roots were randomly allocated to one of four groups (n=20): ProTaper™, WaveOne™, control A, or control B. The files used were S1 and S2/F1 and F2 (ProTaper™ system) and 25.08 (WaveOne™ system). In control group A, the root canal was left uninstrumented, whereas in control group B, the root canal was irrigated with NaCl 0.9%. E. faecalis was sampled from the root canal system before and after instrumentation and the Wilcoxon test and Mann–Whitney U were used. Results: There were no differences in E. faecalis counts between pre-instrumentation counts in the ProTaper™ and WaveOne™ (p>0.05). The ProTaper™ system led to an 89.36% reduction in E. faecalis burden, versus 78.10% with the WaveOne™ system (p>0.05). Instrumentation time was shorter with WaveOne™ (p<0.0001). Conclusions: The ProTaper™ and WaveOne™ systems were equally effective in reducing Enterococcus faecalis in primary molars. The WaveOne™ system was associated with shorter instrumentation time.

Influence of ultrasonic activation on photodynamic therapy over root canal system infected with Enterococcus faecalis – an in vitro study

2014 ◽  
Vol 11 (4) ◽  
pp. 472-478 ◽  
Author(s):  
Guilherme Cavagnoli Ghinzelli ◽  
Matheus Albino Souza ◽  
Doglas Cecchin ◽  
Ana Paula Farina ◽  
José Antônio Poli de Figueiredo
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
In Vitro Study ◽  
Vitro Study ◽  
Canal System ◽  
Ultrasonic Activation ◽  
Root Canal System

scholarly journals Effectiveness of photodynamic therapy and sodium hypochlorite on root canal system infected with Enterococcus faecalis – An in vitro study

2016 ◽  
Vol 31 (3) ◽  
pp. 114
Author(s):  
Matheus Albino Souza ◽  
Liviu Steier ◽  
Giampiero Rossi-Fedele ◽  
Valdir Barth Júnior ◽  
Silvia Dias De Oliveira ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Negative Control ◽  
Canal Wall ◽  
Canal System ◽  
Group 4 ◽  
Root Canal System

Objective: The purpose of this study was to evaluate, in vitro, the effectiveness of photodynamic therapy and sodium hypochlorite over root canal system infected with Enterococcus faecalis.Methods: The root canals of 45 single-rooted human extracted teeth were enlarged up to a file 60, autoclaved, inoculated with Enterococcus faecalis and incubated for 30 days. The samples were divided into five groups (G1 – n=5; G2-G5 – n=10) according to the protocol of decontamination: G1 (negative control) – no procedure was performed; G2 – distilled water; G3 –  hotodynamictherapy (PDT); G4 – 2.5% sodium hypochlorite (NaOCl); and G5 – 2.5% NaOCl+PDT. Scanning electron microscopy (SEM) were performed to evaluate the effectiveness of proposed treatments. The assessment was made by images of each third at 5000x magnification for the canal wall and 10000x for the exposed tubule area. The presence of bacteria was scored by position ranks from 1 to 45, where the higher the value, the cleanliness of the sample. Data were subjected to one-way ANOVA followed by post-hoc Tukey test (α=0.05).Results: Group 4 (2.5% NaOCl) and 5 (2.5% NaOCl + PDT) had the highest mean of position ranks for all thirds of the root canal in the canal wall, which was statistically different from groups 1, 2 and 3 (p<0.05). Group 3 (PDT), group 4 (2.5% NaOCl) and 5 (2.5% NaOCl+PDT) had the highest mean of position ranks in the exposed tubule area, which was statistically different from groups 1 and 2 (p<0.05).Conclusion: the association of 2.5% sodium hypochlorite + photodynamic therapy can be considered an effective protocol for the elimination of Enterococcus faecalis.

scholarly journals ANTIBACTERIAL EFFICACY OF 6% GREEN TEA EXTRACT AND 2% CHLORHEXIDINE AGAINST ENTEROCOCCUS FAECALIS BIOFILM IN VITRO

2019 ◽  
pp. 64-66
Author(s):  
FITRI REFLAN ◽  
RATNA MEIDYAWATI ◽  
DARU INDRAWATI
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Brain Heart Infusion ◽  
Green Tea Extract ◽  
Bacterial Suspension ◽  
Antibacterial Efficacy ◽  
Bacterial Survival ◽  
Planktonic Bacteria ◽  
Tea Extract

Objective: This study aimed to compare the antibacterial efficacy of green tea extract to chlorhexidine 2% against Enterococcus faecalis biofilm usingreal-time polymerase chain reaction (RT-PCR).Methods: E. faecalis strain ATCC 29212 colonies were collected from overnight cultures grown on brain–heart infusion agar (BHIA) and resuspendedat 108 CFU/ml according to a 0.5 McFarland standard. Aliquots of bacterial suspension (50 μl) were then inoculated onto sterile nitrocellulose filterdiscs place on BHIA and incubated aerobically at 37°C for 72 h. After incubation, the discs were removed, transferred into 10-ml phosphate-bufferedsaline (PBS) to loosen planktonic bacteria, and then incubated for 10 min at 37°C in 10 ml of 6% green tea extract, 2% chlorhexidine, or sterile PBS(control). Surviving E. faecalis cells were then quantified by RT-PCR.Results: Green tea extract reduced bacterial survival compared to control but was not as effective as chlorhexidine 2%.Conclusion: Green tea extract may help reduce oral E. faecalis biofilm. Moreover, specific antimicrobial compounds in green tea extract such asepigallocatechin-3-gallate should be tested as non-toxic alternatives to chlorhexidine.

scholarly journals Murine Experimental Root Canal Infection: Cytokine Expression in Response to F. nucleatum and E. faecalis

2016 ◽  
Vol 27 (5) ◽  
pp. 578-583 ◽  
Author(s):  
Andressa Lamari Reis ◽  
◽  
Ricardo Reis Oliveira ◽  
Warley Luciano Fonseca Tavares ◽  
Thamyris Duque Silva Saldanha ◽  
...  
Keyword(s):  
Gene Expression ◽  
Statistical Analysis ◽  
Enterococcus Faecalis ◽  
Experimental Infection ◽  
Root Canal ◽  
Fusobacterium Nucleatum ◽  
High Expression ◽  
Canal System ◽  
Independent Mechanism ◽  
Root Canal System

Abstract The aim of this study was to evaluate the gene expression of proinflammatory (RANKL, TNF-a and IFN-g) and regulatory (TGF-b and IL-10) cytokines as reaction to experimental infection by mono or bi-association of Fusobacterium nucleatum (ATCC 10953) and Enterococcus faecalis (ATCC 19433). F. nucleatum and E. faecalis, either in mono- or bi-association were inoculated into the root canal system (RCS) of Balb/c mice. Animals were sacrificed at 10 and 20 days after infection and periapical tissues surrounding the root were collected. The mRNA expression of the cytokines RANKL, TNF-a, IFN- g, TGF-b and IL-10 was assessed using real-time PCR. The Kruskal-Wallis test was used for statistical analysis. F. nucleatum mono-infection induced high expression of RANKL and TNF-a, while its modulation was due to IL-10. High expression of IFN-g at day 20 was up-regulated by E. faecalis and RANKL; TNF-a was up-regulated by an independent mechanism via IL-10 and TGF-b. Bi-association (F. nucleatum and E. faecalis) stimulated high expression of RANKL, TNF-a and IFN-g, which seemed to be modulated by TGF-b 20 days later. The gene expression of proinflammatory cytokines was more prominent in the earlier periods of the experimental periapical infection, which concomitantly decreased in the later period. This expression may be regulated by IL-10 and TGF-b in an infection-specific condition

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