scholarly journals Molecular detection and phylogenetic analysis of ovine herpesvirus-2 in sheep and goats of Al-Qadisiyah Province

2020 ◽  
Vol 23 (4) ◽  
pp. 424-431
Author(s):  
I. Khudhair ◽  
S. Al-Husseiny ◽  
A. Jawad
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Detection ◽  
Protein Gene ◽  
Nasal Discharge ◽  
Nested Polymerase Chain Reaction ◽  
Sheep And Goats ◽  
Phylogenetic Methods ◽  
Multiple Alignments ◽  
Healthy Sheep ◽  
Polymerase Chain

This study aimed to identify ovine herpesvirus 2 (OHV-2) infections in sheep and goats in Al-Qadisiyah Province of Iraq, using molecular and phylogenetic methods. Nasal discharge swabs were collected from 60 sheep and 60 goats from 3 different animal sale bars. The samples were subjected to semi-nested-polymerase chain reaction (PCR), sequencing, and phylogenetic tests involving OHV-2 tegument protein gene (OHV-2T). The results of the semi-nested PCR showed the presence of OHV-2 in all 60 (100%) sheep and 52 (86.6%) goats. The samples from both sheep and goats were sent for partial-gene-based sequencing to confirm the PCR results. Phylogenetic analysis was conducted and 6 PCR amplicons (10%) of positive samples from each goat and sheep were submitted for sequencing. The sequence results were reassembled and deposited in the NCBI-GenBank database under the accession numbers of MF004402.1 for sheep and MG875327.1 and MG875328.1 for goats. Multiple alignments of sequences showed close identities with some global isolates of this virus. This study not only reports new sequences from the local OHV-2 isolates that have been deposited in the NCBI GenBank, but also provides important data about the presence and shedding of OHV-2 in the nasal discharge of healthy sheep and goats, and suggests OHV-2 as the major cause of malignant catarrhal fever in cattle.

scholarly journals Application of immunofluorescence assay and nested polymerase chain reaction for query fever diagnosis in animal handlers of Puducherry, South India, and phylogenetic analysis based on IS1111 repetitive gene element

2019 ◽  
Vol 12 (11) ◽  
pp. 1769-1774 ◽  
Author(s):  
Jothimani Pradeep ◽  
Selvaraj Stephen ◽  
Balakrishnan Sangeetha ◽  
Prabakar Xavier Antony ◽  
S. Amsaveni ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Tamil Nadu ◽  
South India ◽  
Molecular Evidence ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Gene Element ◽  
Animal Handlers

Background and Aim: Diagnosis of query fever (QF) is mostly done on the basis of serological/molecular tests, due to the stringent requirement of biosafety level-3 containment facilities for isolating Coxiella burnetii in culture. QF is an important zoonosis and is considered to be an occupational hazard to livestock handlers. This report describes our study on the serological as well as molecular evidence of QF in animal handlers from Puducherry and surrounding Tamil Nadu, from where, to the best of our knowledge, no such reports are available so far. Materials and Methods: Seventy-five animal handlers were recruited, comprising veterinarians, slaughterhouse workers, butchers, and animal attendants of various government veterinary clinics from Puducherry and surrounding areas of Tamil Nadu state. QF serology was performed to identify Phase I and Phase II immunoglobulin G antibodies to C. burnetii. Nested polymerase chain reaction (N-PCR) was carried out to detect C. burnetii DNA in buffy coat samples by targeting IS1111 gene element. N-PCR-positive samples were sequenced and phylogenetic analysis was performed using MEGA software version 10.0. Results: A total of 21 animal handlers (28.1%) were positive for either serology or PCR. PCR alone was positive in 10 (13.4%), only serology was positive in 8 (10.7%), and both serology and PCR were positive in three samples (4.0%). GenBank accession numbers were obtained for 13 N-PCR-positive samples (MG548608-MG548620). Six of our study sequences showed close similarity with the reference isolates from Bengaluru, Colombia, Brazil, France, and Iran. Conclusion: A significant percentage of QF positivity in animal handlers of this part of South India, Puducherry, warrants a prospective study with follow-up of a large number of this occupational group.

scholarly journals Detection of ovine herpesvirus-2 in clinical cases of sheep-associated malignant catarrhal fever in balinese cattle and apparently healthy sheep in East Nusa Tenggara

2021 ◽  
Vol 33 ◽  
pp. 06006
Author(s):  
Agus Wiyono ◽  
Harimurti Nuradji ◽  
Maxs UE Sanam ◽  
Yohanes TRMR Simarmata ◽  
Rini Damayanti
Keyword(s):  
Fatal Outcome ◽  
Clinical Signs ◽  
Economic Losses ◽  
Malignant Catarrhal Fever ◽  
Test Results ◽  
Nested Polymerase Chain Reaction ◽  
Healthy Cattle ◽  
Healthy Sheep ◽  
Vaginal Swabs ◽  
Polymerase Chain

Malignant catarrhal fever (MCF) is a disease causing a fatal outcome in cattle and generates economic losses worldwide. This study aims to detect the cause of the disease in Balinese cattle showing clinical signs such as high fever, serous ocular mucopurulent nasal discharges, and enlargement of pre-scapularis and pre-femoralis lymphnodes. These cattle were previously housed 50 meters away from a flock of sheep which were brought from Sabu Island 3 months earlier. Samples including blood, ocular, nasal, and vaginal swabs were collected from 22 sheep, 30 goats, 33 clinically healthy cattle (22 Balinese and 11 Ongole cattle), and 3 infected Balinese cattle. Samples were processed and tested using A nested polymerase chain reaction (PCR) test. Results showed t hat 12 sheep out of 22 and 3 out of 3 infected Balinese cattle were positive MCF, suggesting a potential spread of the disease from sheep to Balinese cattle. No goats and Ongole cattle that were positive indicate that these animals are less susceptible to Ovine Herpesvirus-2 (OvHV-2) infection compared to Balinese cattle. The finding of 5 positive samples from 22 healthy Balinese cattle shows the potential of sub-clinical infection of OvHV-2.

scholarly journals Molecular detection and characterisation of mumps virus in cerebrospinal fluid in a Gauteng laboratory

2016 ◽  
Vol 31 (1) ◽  
pp. 29-31
Author(s):  
Marieke Brauer ◽  
Marianne Wolfaardt ◽  
Lynne M. Webber ◽  
Maureen B. Taylor
Keyword(s):  
Polymerase Chain Reaction ◽  
Phylogenetic Analysis ◽  
Cerebrospinal Fluid ◽  
Aseptic Meningitis ◽  
Reverse Transcription ◽  
Molecular Detection ◽  
Mumps Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

The study aimed to determine the presence of mumps virus (MuV) in cerebrospinal fluid (CSF) specimens and to genetically characterise detected MuV strains. A real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the MuV F gene, and characterisation was performed by sequencing of the SH gene. Mumps virus was detected in 1.2% (3/260) of specimens. Phylogenetic analysis of one MuV strain revealed that it clustered with the Jeryl-Lynn and RIT4385 vaccine strains. As far as the authors could ascertain this is the first study to provide viral proof that these vaccine-like strains may be associated with aseptic meningitis.

scholarly journals Molecular evidence of mixed P. vivax and P. falciparum infections in northern Islamic Republic of Iran

2004 ◽  
Vol 10 (3) ◽  
pp. 336-342 ◽  
Author(s):  
S. Zakeri ◽  
S. Mamaghani ◽  
A. A. Mehrizi ◽  
Z. Shahsavari ◽  
A. Raeisi ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Plasmodium Species ◽  
Single Species ◽  
Molecular Evidence ◽  
Islamic Republic ◽  
Mixed Species ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Islamic Republic Of Iran ◽  
Polymerase Chain

This study compared basic microscopy with molecular detection of Plasmodium species. According to thick-film microscopy, 100% of 142 malaria cases in Pars-Abad, Ardebil province, were infected with a single species, P vivax. However, nested polymerase chain reaction [PCR] detected mixed species infections of both P. vivax and P. falciparum in 7.0%. In Maz and eran province, 2/20 blood films were diagnosed with only P. falciparum and 18/20 with only P. vivax. However, nested PCR detected 17/20, 2/20 and 1/20 with P. vivax only, P. falciparum only and mixed species respectively. The unexpected presence of P. falciparum urges prompt investigation and immediate treatment of malaria cases in this region

scholarly journals Molecular detection and phylogenetic analysis of Mycoplasma Spp. isolated from awassi sheep in Al-Muthana Province, Iraq

2020 ◽  
Vol 23 (1) ◽  
pp. 21-28
Author(s):  
Q. H. KSHASH ◽  
L. A. AL-RAOW
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Detection ◽  
Molecular Study ◽  
Mycoplasma Species ◽  
Rrna Gene ◽  
High Similarity ◽  
Chain Reaction ◽  
Awassi Sheep ◽  
Polymerase Chain ◽  
Mycoplasma Spp

This study was carried out for molecular detection and phylogenetic analysis of Mycoplasma spp. in Awassi sheep in Al-Muthana province, Iraq. A total of 270 milk samples and swabs were collected from infected sheep. A polymerase chain reaction (PCR) technique was performed to detect a specific 16S rRNA gene of Mycoplasma spp. in these samples. Forty-four positive samples (16.2%) were identified, from which eight samples were selected for partial-gene sequencing. Then, alignment, com-parison with referencing isolates in GenBank, and phylogenetic tree were performed using mean (UPGMA tree) in a MEGA software. The analyses revealed high homology between the current Iraqi isolates and American and Sweden Mycoplasma strains. The present molecular study showed that the studied Iraqi Awassi sheep were infected with Mycoplasma spp. with higher detection percentage from ocular swabs than from other types of samples. The phylogenic analysis registered eight Iraqi isolates with accession numbers in the GenBank with high similarity to five referencing Mycoplasma species.

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